FastQCFastQC Report
Sat 31 Dec 2022
SRR5849521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113671
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT9360.823429018835059RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC8150.7169814640497576No Hit
AGTCCCGGGATAGTCTTAATGGCAGAAGCTCTGGAAAAGCTCTTCTTGCAAA1910.16802878482638492No Hit
TGACCAGGGAGACATGGCATACCAAAATGTATGGGATAAAGCTCAAGCAATG1880.1653895892531956No Hit
TGAATAGGGCCACAAAGATGTAATCCAAATCTATTTTTCTCACGTGTTTAAA1690.14867468395633013No Hit
AACAAAGGGGATCTGTTTGCGGGGATCTCAGCAAGGGCGATCTAGTCGTGGG1690.14867468395633013No Hit
CGTTAAGGGACCGTTATAAAACTTTTATGCTCTCATTTTAGTGGGTGTTTTA1670.14691522024087056No Hit
CATTCCGGGGCGGAGAAGGGGTGCGAGCAGCGGCGGCGGCGGAGGCTGCCAT1660.14603548838314082No Hit
ATAGGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1640.14427602466768127No Hit
CCATCTGGGTGCAAAGAAGGGGCAGCAGCCTCCAGAAGGCAGGGCTGATGGA1640.14427602466768127No Hit
CGACGGGGTGACAATTTCCATTTCATCAGTTTGTTTTGTTTTCCTTTTTCTT1630.14339629280995153No Hit
AGTTAAGGGAACCAATGTAATAAGTGAAGAAAAAAATAAGTCCCAGTGATGA1630.14339629280995153No Hit
GATACCGGGGGGCAGAGCCGGTGCGACGTTTCCCTTTCCAGCTTTGTTCTTC1630.14339629280995153No Hit
ATCAACGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTGG1610.141636829094492No Hit
TTACTAGGGAAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCA1600.14075709723676222No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1580.1389976335213027No Hit
ACTAGGGGTGGGTATGAAGCAGTGACTTGGATTCGCCTTGTTTAGGTTATAG1570.13811790166357293No Hit
CGGTCTGGGCGGGAGGGGGAAACCATACCCTGAAGTCCAGTCATTGACAAAT1550.13635843794811342No Hit
AGTAACGGGTGCGGGCTCTCATGGAGCTCAGGTCCACAGATGCATTACGAAA1550.13635843794811342No Hit
ACATTGGGGGATCCGGGGCACGGTGCTCACCTACCAGTGCGAGCCTGGCTAC1550.13635843794811342No Hit
GCAGTTGGGACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGGCC1550.13635843794811342No Hit
GCATATGGGTAAGTGGTAGCTGTTTTGATGATGTATTGCTACCGTGACAGAT1530.13459897423265388No Hit
TACTTTGGGTATCAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAG1500.1319597786594646No Hit
TTCGTGGGGAAGCACATGATGATTTTGTGGTCCTTTATGGTTTCTATTACAT1500.1319597786594646No Hit
GATTCAGGGCTTTGCTATTTTCCCAATTTACTTGGTTACAGTTTGACTGCTC1500.1319597786594646No Hit
GGTCTGGGGGCGGCTGAGGCGCGTGCTCTCGCGTGGTCGCTGGGTCTGCGTC1500.1319597786594646No Hit
TGTTGCGGAATCCAGTGACTGTCAGGTCGATCATATCCATGTTTGACCGTGA1490.13108004680173485No Hit
CGCATAGGGTCTGGTTTCCTTCACTTAGCATAATGTCTTCAATGCCCATCCA1490.13108004680173485No Hit
GCGTTTGGGATGACGTGTCAGGCAGGAATAGACTGCTGCCCATAGAAAGGTA1480.13020031494400508No Hit
AGCGTAGGGCTGTACTCTTATTCAGTTCCTTGGTGATGCTATTGTTTTGCCA1470.1293205830862753No Hit
GCTTCCGGGATATTTGATAATAGAGAAGGGAGTTTTATGGAAGTTTCTTTGA1470.1293205830862753No Hit
GCTAACGGGATGTGATGCGAAAACTGATAGAACTACAAGGGGAAATAGATGA1470.1293205830862753No Hit
CGACATGGGCGTGGGATGGTCTTGATTCAGGGAAGCTCTACACCTTGGTCCT1460.12844085122854554No Hit
TACGTCGGGGAATTGTTGTTATAGAGTGTGTGCTTTTTCAGCTTTACCAGAT1460.12844085122854554No Hit
TTCTTCGGGGTGTGTTGTGCATTTGTATGTTTGTTCAATGAGTCACTTACCC1460.12844085122854554No Hit
CAGCAAGGGCGTGGATCAAAACGAGGTCTTCCAGTTCTGCGGGTCAGAAGGC1460.12844085122854554No Hit
GCATAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1460.12844085122854554No Hit
GTATTTGGGATAGTCTTTGCTATGACTTCTGGCCAGATGTGGAACCATATCC1450.12756111937081577No Hit
ATAAAGGGGATCCTGCCCCCTAAAAACAAAAGGTTTTGATCACAAGGGGAAA1450.12756111937081577No Hit
ATTGCAGGGAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTT1450.12756111937081577No Hit
ATTTCTGGGAGGCAGGGACCCGATTCTTTCCTTGAGCCTCCAGAAGGGACAC1450.12756111937081577No Hit
CCTTGGGCCCTTACCGCCTTCCCCGCCACACCCGCTCTGTCTTCCCACTGTC1430.12580165565535623No Hit
AAATTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1390.12228272822443718No Hit
ACACCTGGGATGCCAGAAAAAGAGATGAAGACCAAATATATATTTAACAATA1390.12228272822443718No Hit
GTGTCGGGGAGTCGTCACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCG1390.12228272822443718No Hit
GCATTTGGGGAAGTCTCAGTTGCAGCTATTTGTCAAAATTAATATCCATTTC1380.12140299636670743No Hit
GCTCATGGGAGTTCAGCCAGTTTCGGAAGAGGGAAGAAAATCTAAATCCAAA1370.12052326450897766No Hit
TCTTACGGGTCCGATCTACAGGGGGGATATGTTTCTGGTTTTCAAAGTTTGC1370.12052326450897766No Hit
AGGCATGGGGGGGGAAGTGTAGTGAGGAGGAGCCCAGAGGACCCCAGGGGAG1370.12052326450897766No Hit
AGTAAAGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1370.12052326450897766No Hit
GTCCTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1360.1196435326512479No Hit
CTACTAGGGATTTCTTTAAAAGGTAATCATTTGTCAAGAGTAAAACCCAGAA1350.11876380079351813No Hit
ATAGGTGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTA1350.11876380079351813No Hit
TCAACTGGGACCAAAATCAAGATAATGAGCATCTACATCACACCAAAAGTTT1350.11876380079351813No Hit
GGTACCGGGAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAG1350.11876380079351813No Hit
TAGGCCGGGGTGAAGTGACTTGGCCAATGGCACATACCATGTTAGTGACAAT1350.11876380079351813No Hit
GCATTGGGGATAGGAAGTATTCTTTTTCTTTACGGCACTCAACTAGTATTTC1350.11876380079351813No Hit
GATACAGGGATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGA1350.11876380079351813No Hit
GAGGTGGGAGGATTGCTTGAGCCCAGGAGGCAGAGGTTGCAGTGAGCCAAGG1340.11788406893578837No Hit
CTTACAGGGCGGGAAGCTTACATTGTACTTATTTTTTTATATCTGAATTTTA1330.11700433707805862No Hit
TGCCTTGGGGAATATTAGGCCGGAGGTCAGCCGGCTGGCAAGACAGTGCAGG1320.11612460522032884No Hit
TCACCTGGGTGGTGGGGGCAGAGGAGTGTCTGCAGGGAAACAGCTTCTCCTC1320.11612460522032884No Hit
GCATAGGGGCAGGTTTGCGCCTCCGCTGGTGATCAAGGAGGATGAGCTTCGA1320.11612460522032884No Hit
ATTATGGGGATCAAATACATATTATGAATATGTGTTTCAATTTTCTGCATTT1310.11524487336259909No Hit
ATAGCTGGGCCTTAATAACCAGTAAAGTTATATTTACACTGGACATTTTAAG1310.11524487336259909No Hit
ACATACGGGTAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTAT1310.11524487336259909No Hit
TCCATAGGGAGTGGATTATATAACCACACATTATAAGGGGCCAAGAATAGTG1300.11436514150486932No Hit
AGCCGAGGGTTCCTTTCCAAATGGGAATTAGAACTGTGGGGCAGCAACAGTC1300.11436514150486932No Hit
ACGCTGAGGGTAGATTGAAATGCTGTATTTCTCCAAAGTTAGGATGCTTAAA1300.11436514150486932No Hit
TTGGCACGGGCTCCTGGGCGTTTTATAGTTTCGTATTTTACATCTGGGCCTA1290.11348540964713956No Hit
CTTCGGGGCACCCGTGGGTCTGTCAACGATCCACGGTGGCATCCATGATGCA1290.11348540964713956No Hit
AAATCCGGGGGGAGCGGGTAAGGATTCGGCGGGCAAGCGGGTGTAATCAGCA1290.11348540964713956No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCT1290.11348540964713956No Hit
ACAGCTGGGATGGGGCACCAGCCTTGCTCAGAACCACCCAAACTCCACAGAG1290.11348540964713956No Hit
CTGAATGGGTGATTATTCCTACAAGTGAAACACTAGACTATTTGGAGTGTAT1290.11348540964713956No Hit
AATCCAGGGATGGAGAGGCTGACTCTTGCTTGTGGTGGGGTAGCCCTGAATT1280.11260567778940979No Hit
GATTAGGGGATCTTCCAGATTGGTTTAACCAATTACTCGTCCCCGCCATTCC1280.11260567778940979No Hit
TCGATAGGGCTTATTCCAGCACTGCAGTGTTTTGACTAAAAATTTTTCATGT1280.11260567778940979No Hit
GACTTCGGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGG1280.11260567778940979No Hit
ATTGCAGGGGGTATTTGATTGTCACAACTAGAGGATATAACAGGAGGTCAGT1280.11260567778940979No Hit
GACCTGGGCGTTGCCAGCAGTTGATTTACAGTGGTCACAGGGCCCAATTCTA1280.11260567778940979No Hit
GTAGTAGGGCCAGTCTTTGTTTTTCTGTTACCATAATGTAAGAGAAGGTCCT1270.11172594593168003No Hit
CTGTAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1270.11172594593168003No Hit
AAGTTTGGGATCACTGGTTGATATTTTTCTACTCTGAGTTTGAGAAGCACAG1270.11172594593168003No Hit
TCCACCGGGATATAATAACTATGATCAAAAAGCTATTTGGAATAGTTTGATT1270.11172594593168003No Hit
TTCGAAGGGAACAGCTCAAAATAATATTTTTTAATTCAGTAGAACTAATAGC1270.11172594593168003No Hit
CAGCAGGGCAATACACCAAAAGCAGCCTAGGGATGAGCATTTCTTTGAAAGC1270.11172594593168003No Hit
TAATGCGGGGTAGCAAGGGTCAGAGAAGACAATAGGTGACATGTTTTTGTAC1270.11172594593168003No Hit
TGCATGGGATGTGAAAAGACCAAATCTACGTCTGACTGGTGTACCTGAAAAT1270.11172594593168003No Hit
GTAGGTGGGTAATATTTTAAGTAACTTCTCAGGAGATCACCCCACAACAGAA1270.11172594593168003No Hit
AAAGCTGGGCCAGATCTTTTCTAATATGGTATTACAATGAAAAGAATAAAGA1260.11084621407395026No Hit
TGCTCTGGGATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTGGG1260.11084621407395026No Hit
AACCTTGGGAAGGCTAAAGCTTAAGGATAATTTTAGACTGTAAATTTGCAGT1260.11084621407395026No Hit
TCTATTGGGGGGGAGCTGAGGGAGGGGAGAGAGAAAAAGAGTGAGAGGGAAA1260.11084621407395026No Hit
GCGACAGGGCAAGACTCCGTCTAAGAAAAAAAGAAATGGTTGAAAATTTTTT1260.11084621407395026No Hit
GAAAAGGGAATAGTGGGAGACTTTAACACCCAACTGTCAATATTAGATGGAT1260.11084621407395026No Hit
TCGCCCGGGACCAAAGATGGCATTGACCACAGTAACATTGGCAACAAGATGC1260.11084621407395026No Hit
GAGATAGGGCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGC1250.1099664822162205No Hit
TTAAAAGGGCGATCTCAGTAGGGGATGACAGATAAATCCTTAACATTTCTCT1250.1099664822162205No Hit
GTCTTAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1250.1099664822162205No Hit
GAAACGGGGCGAACTCCTGACCTTGTGATCTGCCTATCTCGGCCTCCCAAAG1250.1099664822162205No Hit
AGCCTAGGGAAGCAACACCTGGCCAGGCAGTTTAAGGCGATAAAAGCGAAAA1250.1099664822162205No Hit
ATATAGGGGGAGGTCTCCCAAGATAGGCTGCAAAGGCTGGTATTGTTGGAAG1240.10908675035849075No Hit
TCGTACGGGGCTATCTTTTGGGTTCATTCCAAATAGTTTTGTGCCATTGAAA1230.10820701850076096No Hit
CAGTATGGGATAATAGCTCTGCTGCAGTTCTGTGACTTCCAAGCCAGGAATT1230.10820701850076096No Hit
CATCTTGGGTAATGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCT1230.10820701850076096No Hit
AGCAGGGGGGTACAGGGATTTCTGCAGCTAAGCTGTACAAATCAATAAAATT1230.10820701850076096No Hit
TAGTTGGGGTAAAGACACCAAAAGTTCTACTGAGAGCTCTCAGCATACAGAT1230.10820701850076096No Hit
AGAACCAGGGACCTTTCACATTTGCAAGTTTAAAGCCCTGAGAAGTCTTACT1230.10820701850076096No Hit
TCATAGGGGCAGCTATAATCTTAAGGTAAATGGGAAATAGACATAAGTGCAT1220.10732728664303119No Hit
CCACAGGGGCGATCTTCTAGAGGGGCACTCTAGCCTTAGTCCAATATTGATT1220.10732728664303119No Hit
TCTTCATGGGACCAACAACAAACTAGCAGAAAAGAAATCAGTCTCATTTACA1220.10732728664303119No Hit
ATGTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1220.10732728664303119No Hit
TATTGTGGGAAGCCAGACTTGAAGCATGTCAAAAAGGTGTACAGCTGTGACC1220.10732728664303119No Hit
TCTTAGGGTATCCCATGAGGCTAGGAGACAGGAGTCCTGGGTTTGAGCCCTG1220.10732728664303119No Hit
TACGCGCGGGACTCCAGTATATATTGAAATGAAAATAAATCCTGCTCTGACA1220.10732728664303119No Hit
TAAACAGGGATTTCTAGAAATTCTGTTTTTCAAATATGACTTTTTTCTGTTT1220.10732728664303119No Hit
GCACAGAGGGATATTCGGGTTTTCAGACCACCCAACTACTCTGGTACCATTG1210.10644755478530143No Hit
TTCTCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1210.10644755478530143No Hit
TTGTCTGGGATTAATCTAGCATTAATTCCCCCCAGAGGGAATGATATTTTTG1200.10556782292757166No Hit
CGCTCAGGGGCAGCCTTGTGATGTTTGGAGCATAGGTTGCATTCTTATTGAA1200.10556782292757166No Hit
TTTCGCGGATTGAAAGTAGTGCTGTAAATATTCTGCCATAGGAATACTGTCT1190.10468809106984191No Hit
AAACAAGGGCAGGCACCAGGCCTGGTCCAGGAGGAGCTGTGGACACAGTCGT1190.10468809106984191No Hit
CGTGCAGGGGTACATATATTTCTTAAATATTGCAATTGCCTAAATGTTGTAT1190.10468809106984191No Hit
TCATGGGGGCCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGG1180.10380835921211214No Hit
TAATTTGGGAGCACAAACTTGCTAGCTAATTAGAGTTTATCTTTTTAGAAAG1180.10380835921211214No Hit
GGTCAGGGGATGGCAGGGTGTGGTGGCTCAAGCCTGTAATCCCAGCACTTTG1180.10380835921211214No Hit
GACTTGGGGGATATGCCTGTGTGTTTAGCCTGAAGAATCCCACTTTTCTCTG1170.10292862735438238No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1170.10292862735438238No Hit
AGTATAGGGAAACATCTTGTGGGGAAAAGTGACTTTAGATTATGAACTCAAT1170.10292862735438238No Hit
GACCATGGGAATAAAGATACCGAGCACATGTTGTTGTGTTAGTTTAAAACGA1170.10292862735438238No Hit
ATGAAAGGGATAGAAAAAAACATTCTATCTACTGATTTGGGCTGAATGTATG1170.10292862735438238No Hit
TAGCTGGGGACTAGGCGTTGCCGTAAACGTCAGAAAGAGCATTTACGACGGC1170.10292862735438238No Hit
TTTAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGAC1170.10292862735438238No Hit
GAACGCGGGCCAGGATAGCTTTATGAATTGCTCTGACAGCATCATCAATGGT1160.10204889549665262No Hit
GTTTTGGGGATATTAGTTGAATCGTTGGAATAGTATTGTCCCACTAAGAGAA1160.10204889549665262No Hit
GTCATGGGGGTCGCCGCCGGCAGCAGCCTCCCGGCGCGTCTAGGAGCCATGT1160.10204889549665262No Hit
TTTAGAGGGGACAGCCTGTCGGCTGACGTGTCTGCAGTTCCTCCGCGTCTAC1160.10204889549665262No Hit
GAGTGAGGGCGATCTCATTTCGGGACGACAGGTGCTATGAGAACTGTGGCAA1160.10204889549665262No Hit
TAGAAAGGGCAAATACTTTTGCATTAAAAAAGTATGTATTTCTGACTATAAT1150.10116916363892285No Hit
GTCACTGGGACTTTAGGGACTTCCACAATTATGTTCTGATCAATCCTAGGTT1150.10116916363892285No Hit
GCCATCGATTGGGGGTGGTTTTTGTCAAATATACTGTTGATAAATATTTATT1150.10116916363892285No Hit
TGTTAGGGGAACTAGTCACACTGGGAGTCATGAATGTCTTTCCAATAATTCA1140.1002894317811931No Hit
TTTGCTGGGATCACCAATAAAAGAACAGACTGACATCACGTGCCCCCTTATG1140.1002894317811931No Hit
ACACAAGGGGCTAGGGAACAAATTAGAGTTGTTGTTATTTTACACTTACCAT1140.1002894317811931No Hit
CATTCCGGGAAACAAAATTTTGGAAGCTGAAAAGCAGGTAGATGAGTGGTAA1140.1002894317811931No Hit
GCAATAGGGCCCCCGCCCGCAGTGCGGCTCGGCGGAGTACATGGCCCCGGAG1140.1002894317811931No Hit
GTCCTAGGGCCTAACTTTTCAATGACATTTTTTTTAACTACTATATTGATTG1140.1002894317811931No Hit
TGGCTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1140.1002894317811931No Hit
CTTAGCGGGAGGAGCCGTCCTGGGTGCATAGGACCAGTTTCTGTGACTTTTC1140.1002894317811931No Hit
TCAAAAGGGATATGTAAGCTGAATACAAGCTAGGAAGCAAAATAAAAATTAC1140.1002894317811931No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)