FastQCFastQC Report
Sat 31 Dec 2022
SRR5849520.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849520.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114244
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT9250.8096705297433563RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC7680.6722453695598893No Hit
GCGTTTGGGATGACGTGTCAGGCAGGAATAGACTGCTGCCCATAGAAAGGTA1860.1628094254402857No Hit
CGGTCTGGGCGGGAGGGGGAAACCATACCCTGAAGTCCAGTCATTGACAAAT1840.16105878645705682No Hit
CATTCCGGGGCGGAGAAGGGGTGCGAGCAGCGGCGGCGGCGGAGGCTGCCAT1840.16105878645705682No Hit
TGACCAGGGAGACATGGCATACCAAAATGTATGGGATAAAGCTCAAGCAATG1830.1601834669654424No Hit
AGTCCCGGGATAGTCTTAATGGCAGAAGCTCTGGAAAAGCTCTTCTTGCAAA1810.1584328279822135No Hit
GGTCTGGGGGCGGCTGAGGCGCGTGCTCTCGCGTGGTCGCTGGGTCTGCGTC1760.15405623052414133No Hit
GCATTGGGGATAGGAAGTATTCTTTTTCTTTACGGCACTCAACTAGTATTTC1750.15318091103252687No Hit
AAATCCGGGGGGAGCGGGTAAGGATTCGGCGGGCAAGCGGGTGTAATCAGCA1730.151430272049298No Hit
CCATCTGGGTGCAAAGAAGGGGCAGCAGCCTCCAGAAGGCAGGGCTGATGGA1680.14705367459122579No Hit
TGTTGCGGAATCCAGTGACTGTCAGGTCGATCATATCCATGTTTGACCGTGA1630.14267707713315358No Hit
GCAGTTGGGACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGGCC1630.14267707713315358No Hit
GATTAGGGGATCTTCCAGATTGGTTTAACCAATTACTCGTCCCCGCCATTCC1630.14267707713315358No Hit
ACTAGGGGTGGGTATGAAGCAGTGACTTGGATTCGCCTTGTTTAGGTTATAG1620.14180175764153918No Hit
AGTTAAGGGAACCAATGTAATAAGTGAAGAAAAAAATAAGTCCCAGTGATGA1610.14092643814992473No Hit
GATACCGGGGGGCAGAGCCGGTGCGACGTTTCCCTTTCCAGCTTTGTTCTTC1600.14005111865831027No Hit
TTACTAGGGAAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCA1600.14005111865831027No Hit
CGACGGGGTGACAATTTCCATTTCATCAGTTTGTTTTGTTTTCCTTTTTCTT1590.13917579916669584No Hit
AACAAAGGGGATCTGTTTGCGGGGATCTCAGCAAGGGCGATCTAGTCGTGGG1580.13830047967508138No Hit
AGAACCAGGGACCTTTCACATTTGCAAGTTTAAAGCCCTGAGAAGTCTTACT1580.13830047967508138No Hit
AGTAACGGGTGCGGGCTCTCATGGAGCTCAGGTCCACAGATGCATTACGAAA1570.13742516018346698No Hit
TTAAAAGGGCGATCTCAGTAGGGGATGACAGATAAATCCTTAACATTTCTCT1570.13742516018346698No Hit
ACAGCTGGGATGGGGCACCAGCCTTGCTCAGAACCACCCAAACTCCACAGAG1550.1356745212002381No Hit
TATACGCGGGCGTGAAGCCAGCAATTGTTTCGCAATTCGGCATTTTGAAAAC1540.13479920170862364No Hit
CGTTAAGGGACCGTTATAAAACTTTTATGCTCTCATTTTAGTGGGTGTTTTA1540.13479920170862364No Hit
GTAGGTGGGTAATATTTTAAGTAACTTCTCAGGAGATCACCCCACAACAGAA1540.13479920170862364No Hit
GCTAACGGGATGTGATGCGAAAACTGATAGAACTACAAGGGGAAATAGATGA1540.13479920170862364No Hit
GGTACCGGGAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAG1530.1339238822170092No Hit
TGAATAGGGCCACAAAGATGTAATCCAAATCTATTTTTCTCACGTGTTTAAA1520.13304856272539478No Hit
ATATGCGGGACCACATGGCTTTTGATGATTCTGGATTCTAAACAACCCAGAA1520.13304856272539478No Hit
AAATTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1500.1312979237421659No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1500.1312979237421659No Hit
AGCGTAGGGCTGTACTCTTATTCAGTTCCTTGGTGATGCTATTGTTTTGCCA1490.13042260425055144No Hit
TCTTCATGGGACCAACAACAAACTAGCAGAAAAGAAATCAGTCTCATTTACA1480.129547284758937No Hit
TTTAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGAC1480.129547284758937No Hit
CTGAATGGGTGATTATTCCTACAAGTGAAACACTAGACTATTTGGAGTGTAT1480.129547284758937No Hit
CTGTAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1470.12867196526732258No Hit
GTGTCGGGGAGTCGTCACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCG1470.12867196526732258No Hit
ATTATGGGGATCAAATACATATTATGAATATGTGTTTCAATTTTCTGCATTT1460.12779664577570812No Hit
ATTTCTGGGAGGCAGGGACCCGATTCTTTCCTTGAGCCTCCAGAAGGGACAC1460.12779664577570812No Hit
GCATATGGGTAAGTGGTAGCTGTTTTGATGATGTATTGCTACCGTGACAGAT1450.1269213262840937No Hit
AGCCTAGGGAAGCAACACCTGGCCAGGCAGTTTAAGGCGATAAAAGCGAAAA1450.1269213262840937No Hit
TATGATGGGCAAGACATCAGTTGATCATTTTTCAAGAAAATGTAGCCTAAAT1440.12604600679247924No Hit
TTTGCTGGGATCACCAATAAAAGAACAGACTGACATCACGTGCCCCCTTATG1440.12604600679247924No Hit
GTCTTAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1440.12604600679247924No Hit
GTCCTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1440.12604600679247924No Hit
ACACCTGGGATGCCAGAAAAAGAGATGAAGACCAAATATATATTTAACAATA1440.12604600679247924No Hit
ATAGGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1430.12517068730086484No Hit
TCAACTGGGACCAAAATCAAGATAATGAGCATCTACATCACACCAAAAGTTT1430.12517068730086484No Hit
TGTTAGGGGAACTAGTCACACTGGGAGTCATGAATGTCTTTCCAATAATTCA1410.12342004831763594No Hit
CCTTGGGCCCTTACCGCCTTCCCCGCCACACCCGCTCTGTCTTCCCACTGTC1410.12342004831763594No Hit
CTTCGGGGCACCCGTGGGTCTGTCAACGATCCACGGTGGCATCCATGATGCA1410.12342004831763594No Hit
TTTAGAGGGGACAGCCTGTCGGCTGACGTGTCTGCAGTTCCTCCGCGTCTAC1400.12254472882602149No Hit
TACGTCGGGGAATTGTTGTTATAGAGTGTGTGCTTTTTCAGCTTTACCAGAT1390.12166940933440706No Hit
TACGCGCGGGACTCCAGTATATATTGAAATGAAAATAAATCCTGCTCTGACA1390.12166940933440706No Hit
GTCCTAGGGCCTAACTTTTCAATGACATTTTTTTTAACTACTATATTGATTG1390.12166940933440706No Hit
GGGCATGGGGGGATAAAGGCCAAATATATATTTCACAATATCACAATACACT1380.12079408984279262No Hit
TCTTACGGGTCCGATCTACAGGGGGGATATGTTTCTGGTTTTCAAAGTTTGC1380.12079408984279262No Hit
TTGGCACGGGCTCCTGGGCGTTTTATAGTTTCGTATTTTACATCTGGGCCTA1380.12079408984279262No Hit
AAATAAGGGTTACAAAGAAGATTTAAAACCGCTACTTAATACACTTGGGCTC1380.12079408984279262No Hit
GATACAGGGATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGA1380.12079408984279262No Hit
ACAACTGGGCTCTATGATATAATGACTCCTTTTGAAATGCTAGAAGAAGAAG1380.12079408984279262No Hit
TTCTGCGGGGAACTGTTTTGCTGTAGACCTCTCTTCTTTGTTTTCAGGAGGT1370.11991877035117818No Hit
GCCATCGATTGGGGGTGGTTTTTGTCAAATATACTGTTGATAAATATTTATT1370.11991877035117818No Hit
GTCACTGGGACTTTAGGGACTTCCACAATTATGTTCTGATCAATCCTAGGTT1360.11904345085956373No Hit
CGCATAGGGTCTGGTTTCCTTCACTTAGCATAATGTCTTCAATGCCCATCCA1360.11904345085956373No Hit
TCTTTCGGGACAGACTTCTTGTTCTGATGTCACGTCCTTGTTCACCAAACAG1350.11816813136794929No Hit
TCACCTGGGTGGTGGGGGCAGAGGAGTGTCTGCAGGGAAACAGCTTCTCCTC1350.11816813136794929No Hit
TACTTTGGGTATCAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAG1340.11729281187633488No Hit
GAAAAGGGAATAGTGGGAGACTTTAACACCCAACTGTCAATATTAGATGGAT1340.11729281187633488No Hit
CAGCAGGGCAATACACCAAAAGCAGCCTAGGGATGAGCATTTCTTTGAAAGC1340.11729281187633488No Hit
TAGTTGGGGTAAAGACACCAAAAGTTCTACTGAGAGCTCTCAGCATACAGAT1340.11729281187633488No Hit
TGCATGGGATGTGAAAAGACCAAATCTACGTCTGACTGGTGTACCTGAAAAT1330.11641749238472043No Hit
AAGTTTGGGATCACTGGTTGATATTTTTCTACTCTGAGTTTGAGAAGCACAG1320.11554217289310598No Hit
ACACAAGGGGCTAGGGAACAAATTAGAGTTGTTGTTATTTTACACTTACCAT1320.11554217289310598No Hit
TCATGGGGGCCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGG1310.11466685340149153No Hit
CATTCCGGGAAACAAAATTTTGGAAGCTGAAAAGCAGGTAGATGAGTGGTAA1310.11466685340149153No Hit
AAACAAGGGCAGGCACCAGGCCTGGTCCAGGAGGAGCTGTGGACACAGTCGT1310.11466685340149153No Hit
GCATAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1310.11466685340149153No Hit
CTTACAGGGCGGGAAGCTTACATTGTACTTATTTTTTTATATCTGAATTTTA1310.11466685340149153No Hit
ACGCTGAGGGTAGATTGAAATGCTGTATTTCTCCAAAGTTAGGATGCTTAAA1310.11466685340149153No Hit
ATCAACGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTGG1300.11379153390987709No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1300.11379153390987709No Hit
GCACAGAGGGATATTCGGGTTTTCAGACCACCCAACTACTCTGGTACCATTG1300.11379153390987709No Hit
GTCATGGGGGTCGCCGCCGGCAGCAGCCTCCCGGCGCGTCTAGGAGCCATGT1300.11379153390987709No Hit
GCTTCCGGGATATTTGATAATAGAGAAGGGAGTTTTATGGAAGTTTCTTTGA1300.11379153390987709No Hit
TTCTCAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1300.11379153390987709No Hit
CTTACAGGGTAACGAAGTGTTTCTTCTTCTGTGAGGAATTAATGGACTTTGA1300.11379153390987709No Hit
GTATTTGGGATAGTCTTTGCTATGACTTCTGGCCAGATGTGGAACCATATCC1290.11291621441826268No Hit
TGGCATTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC1290.11291621441826268No Hit
CAGCAAGGGCGTGGATCAAAACGAGGTCTTCCAGTTCTGCGGGTCAGAAGGC1290.11291621441826268No Hit
CTCACCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1290.11291621441826268No Hit
ATATAGGGGGAGGTCTCCCAAGATAGGCTGCAAAGGCTGGTATTGTTGGAAG1290.11291621441826268No Hit
CGACATGGGCGTGGGATGGTCTTGATTCAGGGAAGCTCTACACCTTGGTCCT1280.11204089492664823No Hit
AATAAGGGAACAAGACTAGCAGGCAATGTGAATGGATATTTATATAATTTTA1280.11204089492664823No Hit
GACCATGGGAATAAAGATACCGAGCACATGTTGTTGTGTTAGTTTAAAACGA1280.11204089492664823No Hit
TAAACAGGGATTTCTAGAAATTCTGTTTTTCAAATATGACTTTTTTCTGTTT1280.11204089492664823No Hit
TCCACCGGGATATAATAACTATGATCAAAAAGCTATTTGGAATAGTTTGATT1270.11116557543503379No Hit
AACCTTGGGAAGGCTAAAGCTTAAGGATAATTTTAGACTGTAAATTTGCAGT1270.11116557543503379No Hit
GCAACTGGGTGGAGGGTCATTAGCAGCATTGAGCAGAAAACCATGGCTGATG1270.11116557543503379No Hit
TCGCCCGGGACCAAAGATGGCATTGACCACAGTAACATTGGCAACAAGATGC1270.11116557543503379No Hit
TAGAAAGGGCAAATACTTTTGCATTAAAAAAGTATGTATTTCTGACTATAAT1260.11029025594341935No Hit
TCAGCAGGGACGGAGGCGCCAGTCCTCAACATTCAGTTCAGGTATGGGGGCA1260.11029025594341935No Hit
GAACGCGGGCCAGGATAGCTTTATGAATTGCTCTGACAGCATCATCAATGGT1260.11029025594341935No Hit
ATGAAAGGGATAGAAAAAAACATTCTATCTACTGATTTGGGCTGAATGTATG1260.11029025594341935No Hit
TAATTTGGGAGCACAAACTTGCTAGCTAATTAGAGTTTATCTTTTTAGAAAG1250.1094149364518049No Hit
ATAAAGGGGATCCTGCCCCCTAAAAACAAAAGGTTTTGATCACAAGGGGAAA1250.1094149364518049No Hit
ATTGCAGGGAACTTAGTATTATACCCACACCCACCCAAGAACAGGGTTTGTT1250.1094149364518049No Hit
ATAGCTGGGCCTTAATAACCAGTAAAGTTATATTTACACTGGACATTTTAAG1250.1094149364518049No Hit
TTCTTCGGGGTGTGTTGTGCATTTGTATGTTTGTTCAATGAGTCACTTACCC1250.1094149364518049No Hit
AGAACGGGATTGACTCATTTATGTCTTAGTCTTTTTTATTACAAATTATTTA1250.1094149364518049No Hit
GAGATAGGGCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTGC1240.10853961696019047No Hit
GCGACAGGGCAAGACTCCGTCTAAGAAAAAAAGAAATGGTTGAAAATTTTTT1240.10853961696019047No Hit
ATTGCAGGGGGTATTTGATTGTCACAACTAGAGGATATAACAGGAGGTCAGT1240.10853961696019047No Hit
TTCGTGGGGAAGCACATGATGATTTTGTGGTCCTTTATGGTTTCTATTACAT1230.10766429746857603No Hit
AGTAAAGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1230.10766429746857603No Hit
CAGTATGGGATAATAGCTCTGCTGCAGTTCTGTGACTTCCAAGCCAGGAATT1220.10678897797696159No Hit
TTCGAAGGGAACAGCTCAAAATAATATTTTTTAATTCAGTAGAACTAATAGC1220.10678897797696159No Hit
GAGCTTGGGATCCAAATTCGAATGGTTCAAGCAGCCGTGAAATCGCTCTTCA1220.10678897797696159No Hit
TAGTGGGGGTGAAGGAAAGGAAATAAATGTTTTCCATATTTCGTCTTGATTT1220.10678897797696159No Hit
AATCCTGGGACTAGTATATCGCTCACACCTCATATCCTCCCTACTATGCCTA1210.10591365848534715No Hit
GTTATGGGGAAACTTGGCTCACTGCAACCTCTGCCCCCTGGGTTCAAGCGAT1210.10591365848534715No Hit
ATCAACGGGAAGTGTGTACTCGCAAAAGAATTTCTGTAGCACAGCATTAGAG1210.10591365848534715No Hit
ATAGGTGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTA1210.10591365848534715No Hit
AACTATGGGTGGATGCTTCAAGAGGTATAATCCTATTTTATTAGCACAAACT1210.10591365848534715No Hit
AGGCATGGGGGGGGAAGTGTAGTGAGGAGGAGCCCAGAGGACCCCAGGGGAG1210.10591365848534715No Hit
TAATGCGGGGTAGCAAGGGTCAGAGAAGACAATAGGTGACATGTTTTTGTAC1210.10591365848534715No Hit
TAGGCCGGGGTGAAGTGACTTGGCCAATGGCACATACCATGTTAGTGACAAT1210.10591365848534715No Hit
AGGCTTGGGTGGATGATAGCGATTGGGTAAATTAACTCTACATTGATATTCA1210.10591365848534715No Hit
CATATGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATT1200.10503833899373272No Hit
ATGTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1200.10503833899373272No Hit
GACCTGGGCGTTGCCAGCAGTTGATTTACAGTGGTCACAGGGCCCAATTCTA1200.10503833899373272No Hit
TCACTTGGGCTTTTTTTCTTCCCCCTCTTTCTTTAAAGAACTGCTCAATATT1190.10416301950211827No Hit
TAGAAAGGGCAAGGAAATAGATTGGAAGCAAAAGAAGGAGGGAAAAGAGTAG1190.10416301950211827No Hit
TAAAGGGGATCTTATTCAGATGATCTTTTAAGGTTTAACTGGTTTTGCTTTT1190.10416301950211827No Hit
AGCAGGGGGGTACAGGGATTTCTGCAGCTAAGCTGTACAAATCAATAAAATT1190.10416301950211827No Hit
ATGGCCGGGCTTACACCTAGCAGGTGTCTCCTCTATCTTAGGGGCCATCAAT1180.10328770001050383No Hit
GCTCATGGGAGTTCAGCCAGTTTCGGAAGAGGGAAGAAAATCTAAATCCAAA1180.10328770001050383No Hit
TTTCGAGGGACACACAGACATGAATTTTTTCCTGTTTGCCAATGCATTATAT1180.10328770001050383No Hit
GATTCAGGGCTTTGCTATTTTCCCAATTTACTTGGTTACAGTTTGACTGCTC1180.10328770001050383No Hit
TTGTCTGGGATTAATCTAGCATTAATTCCCCCCAGAGGGAATGATATTTTTG1180.10328770001050383No Hit
CAAGTAGGGGTCTTGCTACCAGAATCCATTCCTTTCTTAGCAGAGTTGATGG1180.10328770001050383No Hit
GCAATAGGGCCCCCGCCCGCAGTGCGGCTCGGCGGAGTACATGGCCCCGGAG1180.10328770001050383No Hit
GAAAATGGGCTGTCACTTCGCTGCCTTTTTAAAATAGTGTGGTATTTCAAAT1170.10241238051888939No Hit
GAAATTGGGAGAGATGAGACGATTGAGGCCAGAGGGGTGTGGTAACTTGCCT1170.10241238051888939No Hit
TCTAGAGGGCGATCTTCAAGAGGGATCTTTTAACCAACTGAACAATACACCA1170.10241238051888939No Hit
TTTACAGGGACTACCAGATTTACTTCCATCGTTCGTCTTTGTTCTATAGATT1170.10241238051888939No Hit
CTAAATGGGCGACATCCAGAAATGAGCTGATGTCAATGCTATGGACTGCACA1170.10241238051888939No Hit
TATATGGGGTGCGGCGGGGTCTGTCAGTGGGGTTTGCATGGCCATTTTGCAT1170.10241238051888939No Hit
CCACAGGGGCGATCTTCTAGAGGGGCACTCTAGCCTTAGTCCAATATTGATT1160.10153706102727494No Hit
ACCACTGGGATCTCTAAATCTGCAGACATCCTGAGACTGTGCAAATACAGGG1160.10153706102727494No Hit
AAAGCTGGGCCAGATCTTTTCTAATATGGTATTACAATGAAAAGAATAAAGA1160.10153706102727494No Hit
ATTATTGGGAGTCTTCTCTGGAGAGAAAATACAGTTTCCCCTTCCTCTTTCT1160.10153706102727494No Hit
CATCTTGGGTAATGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCT1160.10153706102727494No Hit
CGTGCAGGGGTACATATATTTCTTAAATATTGCAATTGCCTAAATGTTGTAT1160.10153706102727494No Hit
GAAACGGGGCGAACTCCTGACCTTGTGATCTGCCTATCTCGGCCTCCCAAAG1160.10153706102727494No Hit
TGCCTTGGGGAATATTAGGCCGGAGGTCAGCCGGCTGGCAAGACAGTGCAGG1150.10066174153566053No Hit
CTACTAGGGATTTCTTTAAAAGGTAATCATTTGTCAAGAGTAAAACCCAGAA1150.10066174153566053No Hit
TCTCATGGGATGAGTTGTGCCTAAAAGTTAAAACTGTTGACTGTATTATGTA1150.10066174153566053No Hit
TTACGAGGGCAGCTCTGCCTGGCTGGTGTTCAGGCCCTCAGTCACTTTGGAA1150.10066174153566053No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)