FastQCFastQC Report
Sat 31 Dec 2022
SRR5849519.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849519.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111981
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT9250.8260329877389915RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC8200.7322670810226735No Hit
TGACCAGGGAGACATGGCATACCAAAATGTATGGGATAAAGCTCAAGCAATG2170.19378287388039042No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1890.1687786320893723No Hit
CGGTCTGGGCGGGAGGGGGAAACCATACCCTGAAGTCCAGTCATTGACAAAT1800.16074155437083076No Hit
CAGCAGGGCAATACACCAAAAGCAGCCTAGGGATGAGCATTTCTTTGAAAGC1800.16074155437083076No Hit
CGACGGGGTGACAATTTCCATTTCATCAGTTTGTTTTGTTTTCCTTTTTCTT1740.15538350255846975No Hit
AAATTGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1650.14734642483992821No Hit
AGCGTAGGGCTGTACTCTTATTCAGTTCCTTGGTGATGCTATTGTTTTGCCA1650.14734642483992821No Hit
CGTTAAGGGACCGTTATAAAACTTTTATGCTCTCATTTTAGTGGGTGTTTTA1590.14198837302756717No Hit
GCAGTTGGGACCGAACGAAAAATTCTAGGCTATATACAACTACGCAAAGGCC1560.13930934712138668No Hit
TCTTCATGGGACCAACAACAAACTAGCAGAAAAGAAATCAGTCTCATTTACA1530.13663032121520616No Hit
TTAAAAGGGCGATCTCAGTAGGGGATGACAGATAAATCCTTAACATTTCTCT1520.13573731257981264No Hit
TGTTGCGGAATCCAGTGACTGTCAGGTCGATCATATCCATGTTTGACCGTGA1520.13573731257981264No Hit
AGCCTAGGGAAGCAACACCTGGCCAGGCAGTTTAAGGCGATAAAAGCGAAAA1520.13573731257981264No Hit
GTCCTAGGGCCTAACTTTTCAATGACATTTTTTTTAACTACTATATTGATTG1510.13484430394441912No Hit
AGTAAAGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1500.13395129530902564No Hit
GTAGGTGGGTAATATTTTAAGTAACTTCTCAGGAGATCACCCCACAACAGAA1490.13305828667363212No Hit
ACTAGGGGTGGGTATGAAGCAGTGACTTGGATTCGCCTTGTTTAGGTTATAG1480.13216527803823863No Hit
GATTAGGGGATCTTCCAGATTGGTTTAACCAATTACTCGTCCCCGCCATTCC1480.13216527803823863No Hit
TTGGCACGGGCTCCTGGGCGTTTTATAGTTTCGTATTTTACATCTGGGCCTA1480.13216527803823863No Hit
AGTCCCGGGATAGTCTTAATGGCAGAAGCTCTGGAAAAGCTCTTCTTGCAAA1480.13216527803823863No Hit
GGTCTGGGGGCGGCTGAGGCGCGTGCTCTCGCGTGGTCGCTGGGTCTGCGTC1480.13216527803823863No Hit
CCATCTGGGTGCAAAGAAGGGGCAGCAGCCTCCAGAAGGCAGGGCTGATGGA1460.13037926076745163No Hit
CATTCCGGGGCGGAGAAGGGGTGCGAGCAGCGGCGGCGGCGGAGGCTGCCAT1460.13037926076745163No Hit
GCGACAGGGCAAGACTCCGTCTAAGAAAAAAAGAAATGGTTGAAAATTTTTT1450.1294862521320581No Hit
ACAGCTGGGATGGGGCACCAGCCTTGCTCAGAACCACCCAAACTCCACAGAG1450.1294862521320581No Hit
TGAATAGGGCCACAAAGATGTAATCCAAATCTATTTTTCTCACGTGTTTAAA1440.12859324349666462No Hit
GGTACCGGGAACTCCTGAGCCAACAACTTAATATGACTAGCTTACACAATAG1440.12859324349666462No Hit
TTTGCTGGGATCACCAATAAAAGAACAGACTGACATCACGTGCCCCCTTATG1430.1277002348612711No Hit
GAAAAGGGAATAGTGGGAGACTTTAACACCCAACTGTCAATATTAGATGGAT1430.1277002348612711No Hit
CTTACAGGGCGGGAAGCTTACATTGTACTTATTTTTTTATATCTGAATTTTA1420.1268072262258776No Hit
GATACAGGGATCCTCTGATCAGGGTGAGCATCAAACTCAAACTACGCCCTGA1410.1259142175904841No Hit
TTCGAAGGGAACAGCTCAAAATAATATTTTTTAATTCAGTAGAACTAATAGC1390.1241282003196971No Hit
TCTTACGGGTCCGATCTACAGGGGGGATATGTTTCTGGTTTTCAAAGTTTGC1390.1241282003196971No Hit
TTCGTGGGGAAGCACATGATGATTTTGTGGTCCTTTATGGTTTCTATTACAT1380.1232351916843036No Hit
ATAGGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1370.12234218304891008No Hit
TACTTTGGGTATCAGCTTCAGTGACCCAGGGTCTGAACTGCCTCCATCCTAG1370.12234218304891008No Hit
ATGTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1370.12234218304891008No Hit
GCATTGGGGATAGGAAGTATTCTTTTTCTTTACGGCACTCAACTAGTATTTC1370.12234218304891008No Hit
ATATGCGGGACCACATGGCTTTTGATGATTCTGGATTCTAAACAACCCAGAA1360.12144917441351659No Hit
GCATAAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1350.12055616577812307No Hit
GCTTCCGGGATATTTGATAATAGAGAAGGGAGTTTTATGGAAGTTTCTTTGA1350.12055616577812307No Hit
AACAAAGGGGATCTGTTTGCGGGGATCTCAGCAAGGGCGATCTAGTCGTGGG1340.11966315714272957No Hit
TCAAAAGGGATATGTAAGCTGAATACAAGCTAGGAAGCAAAATAAAAATTAC1340.11966315714272957No Hit
AGTAACGGGTGCGGGCTCTCATGGAGCTCAGGTCCACAGATGCATTACGAAA1330.11877014850733605No Hit
AAATCCGGGGGGAGCGGGTAAGGATTCGGCGGGCAAGCGGGTGTAATCAGCA1330.11877014850733605No Hit
CGCATAGGGTCTGGTTTCCTTCACTTAGCATAATGTCTTCAATGCCCATCCA1330.11877014850733605No Hit
TAGAAAGGGCAAATACTTTTGCATTAAAAAAGTATGTATTTCTGACTATAAT1320.11787713987194257No Hit
GTCTTAGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1320.11787713987194257No Hit
TCAACTGGGACCAAAATCAAGATAATGAGCATCTACATCACACCAAAAGTTT1320.11787713987194257No Hit
TAAACAGGGATTTCTAGAAATTCTGTTTTTCAAATATGACTTTTTTCTGTTT1320.11787713987194257No Hit
TGGCATTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGC1310.11698413123654905No Hit
TGCATGGGATGTGAAAAGACCAAATCTACGTCTGACTGGTGTACCTGAAAAT1310.11698413123654905No Hit
GCGTTTGGGATGACGTGTCAGGCAGGAATAGACTGCTGCCCATAGAAAGGTA1310.11698413123654905No Hit
GCATATGGGTAAGTGGTAGCTGTTTTGATGATGTATTGCTACCGTGACAGAT1300.11609112260115556No Hit
AGTATAGGGAAACATCTTGTGGGGAAAAGTGACTTTAGATTATGAACTCAAT1300.11609112260115556No Hit
TTCTTCGGGGTGTGTTGTGCATTTGTATGTTTGTTCAATGAGTCACTTACCC1300.11609112260115556No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1290.11519811396576206No Hit
AGTTAAGGGAACCAATGTAATAAGTGAAGAAAAAAATAAGTCCCAGTGATGA1290.11519811396576206No Hit
TTTACAGGGACTACCAGATTTACTTCCATCGTTCGTCTTTGTTCTATAGATT1290.11519811396576206No Hit
GGGCATGGGGGGATAAAGGCCAAATATATATTTCACAATATCACAATACACT1280.11430510533036854No Hit
ATCAACGGGAAGTGTGTACTCGCAAAAGAATTTCTGTAGCACAGCATTAGAG1280.11430510533036854No Hit
GATACCGGGGGGCAGAGCCGGTGCGACGTTTCCCTTTCCAGCTTTGTTCTTC1280.11430510533036854No Hit
TAAAGGGGATCTTATTCAGATGATCTTTTAAGGTTTAACTGGTTTTGCTTTT1280.11430510533036854No Hit
TCATGGGGGCCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTAGG1270.11341209669497505No Hit
GTCACTGGGACTTTAGGGACTTCCACAATTATGTTCTGATCAATCCTAGGTT1270.11341209669497505No Hit
GACCATGGGAATAAAGATACCGAGCACATGTTGTTGTGTTAGTTTAAAACGA1270.11341209669497505No Hit
ATTTCTGGGAGGCAGGGACCCGATTCTTTCCTTGAGCCTCCAGAAGGGACAC1270.11341209669497505No Hit
AGAACCAGGGACCTTTCACATTTGCAAGTTTAAAGCCCTGAGAAGTCTTACT1270.11341209669497505No Hit
CATCTTGGGTAATGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCT1260.11251908805958154No Hit
GTGTCGGGGAGTCGTCACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCG1260.11251908805958154No Hit
ATCAACGGGCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTTGG1250.11162607942418803No Hit
TGACATGGGGTACTTTATCTGTAATAAACTTTGTAGATCCTGTGAACCATTA1250.11162607942418803No Hit
CATTCCGGGAAACAAAATTTTGGAAGCTGAAAAGCAGGTAGATGAGTGGTAA1250.11162607942418803No Hit
TCTATTGGGGGGGAGCTGAGGGAGGGGAGAGAGAAAAAGAGTGAGAGGGAAA1250.11162607942418803No Hit
AGGCATGGGGGGGGAAGTGTAGTGAGGAGGAGCCCAGAGGACCCCAGGGGAG1250.11162607942418803No Hit
TTACTAGGGAAGAAAAAAGTGACTTTCAACTCTTCTTCCATCATTTTTATCA1250.11162607942418803No Hit
AAGTTTGGGATCACTGGTTGATATTTTTCTACTCTGAGTTTGAGAAGCACAG1240.11073307078879452No Hit
TGTTAGGGGAACTAGTCACACTGGGAGTCATGAATGTCTTTCCAATAATTCA1240.11073307078879452No Hit
CTACTAGGGATTTCTTTAAAAGGTAATCATTTGTCAAGAGTAAAACCCAGAA1240.11073307078879452No Hit
TTTAGAGGGGACAGCCTGTCGGCTGACGTGTCTGCAGTTCCTCCGCGTCTAC1240.11073307078879452No Hit
GCATTTGGGATAGTTTGGTGAAATCATCTTAAATCAAATTTAAATATCACAT1240.11073307078879452No Hit
TGGCTTGGGAATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATT1240.11073307078879452No Hit
AAATAAGGGTTACAAAGAAGATTTAAAACCGCTACTTAATACACTTGGGCTC1240.11073307078879452No Hit
GCCGGCGGCAACGGGAGGAACAGCAGCAGAGGCAGCAGGAGCAGGAGGAGCG1240.11073307078879452No Hit
CTGTAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1230.10984006215340103No Hit
TGCTCTGGGATGCATTGGAGGAAAAACATGTTGGCTTTTCTCTTGACGTGGG1230.10984006215340103No Hit
GCAATAGGGCCCCCGCCCGCAGTGCGGCTCGGCGGAGTACATGGCCCCGGAG1230.10984006215340103No Hit
GCTAACGGGATGTGATGCGAAAACTGATAGAACTACAAGGGGAAATAGATGA1230.10984006215340103No Hit
GTATTTGGGATAGTCTTTGCTATGACTTCTGGCCAGATGTGGAACCATATCC1220.10894705351800751No Hit
TCGTACGGGGCTATCTTTTGGGTTCATTCCAAATAGTTTTGTGCCATTGAAA1220.10894705351800751No Hit
ATAAAGGGGATCCTGCCCCCTAAAAACAAAAGGTTTTGATCACAAGGGGAAA1220.10894705351800751No Hit
GTCATGGGGGTCGCCGCCGGCAGCAGCCTCCCGGCGCGTCTAGGAGCCATGT1220.10894705351800751No Hit
AGCAGGGGGGTACAGGGATTTCTGCAGCTAAGCTGTACAAATCAATAAAATT1220.10894705351800751No Hit
ACTACCGGGGTATCAAAGCCCAACATGACAGGAGCTCAGCGTAGAAGCAGTT1220.10894705351800751No Hit
GCATAGGGGCAGGTTTGCGCCTCCGCTGGTGATCAAGGAGGATGAGCTTCGA1220.10894705351800751No Hit
TTTAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGAC1220.10894705351800751No Hit
CGTGCAGGGGTACATATATTTCTTAAATATTGCAATTGCCTAAATGTTGTAT1210.10805404488261401No Hit
TCGCCCGGGACCAAAGATGGCATTGACCACAGTAACATTGGCAACAAGATGC1210.10805404488261401No Hit
GAGTGAGGGCGATCTCATTTCGGGACGACAGGTGCTATGAGAACTGTGGCAA1210.10805404488261401No Hit
GAGGTGGGAGGATTGCTTGAGCCCAGGAGGCAGAGGTTGCAGTGAGCCAAGG1200.10716103624722052No Hit
AACCTTGGGAAGGCTAAAGCTTAAGGATAATTTTAGACTGTAAATTTGCAGT1200.10716103624722052No Hit
TTCTGCGGGGAACTGTTTTGCTGTAGACCTCTCTTCTTTGTTTTCAGGAGGT1200.10716103624722052No Hit
GCTCAGGGCATCCAAAGAGATAAGGATGAAGACAGTAAAGAACAGAACTCCA1200.10716103624722052No Hit
ACATACGGGTAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCATAATTAT1200.10716103624722052No Hit
ACTCCTGGGACTCTTGCCAAAAGAGAATGGCATTATTACATCCAAGGGAAAT1190.106268027611827No Hit
ACACAAGGGGCTAGGGAACAAATTAGAGTTGTTGTTATTTTACACTTACCAT1190.106268027611827No Hit
ATAGGTGGGGAGAGTGTGGTCAGGCGGCTCGGACTGAGCAGGACTTTCCTTA1190.106268027611827No Hit
GTTTTGGGGATATTAGTTGAATCGTTGGAATAGTATTGTCCCACTAAGAGAA1190.106268027611827No Hit
TACGTCGGGGAATTGTTGTTATAGAGTGTGTGCTTTTTCAGCTTTACCAGAT1190.106268027611827No Hit
CATACAGGGATTTTTGTAGATTAATTGTTGTAACATTGTCTTTCTTTTTTTT1190.106268027611827No Hit
ATTGCAGGGGGTATTTGATTGTCACAACTAGAGGATATAACAGGAGGTCAGT1190.106268027611827No Hit
CAGCTTGGGACAGGAGAGCTGCAGTTGTGAAAGCACCATCATCATAGAGGAT1190.106268027611827No Hit
TCCATAGGGAGTGGATTATATAACCACACATTATAAGGGGCCAAGAATAGTG1180.1053750189764335No Hit
AACTATGGGTGGATGCTTCAAGAGGTATAATCCTATTTTATTAGCACAAACT1180.1053750189764335No Hit
ACACCTGGGATGCCAGAAAAAGAGATGAAGACCAAATATATATTTAACAATA1180.1053750189764335No Hit
TGAAAAGGGGTCCTAGTCGGAGCTTAGGAGGGGCGGAGACGCTCACATCGTC1180.1053750189764335No Hit
CTTGTAGGGAATGCTTGTCACTGTGGAGAAAGAGTTTTGTATATGTCTGATA1180.1053750189764335No Hit
GACTTGGGGGATATGCCTGTGTGTTTAGCCTGAAGAATCCCACTTTTCTCTG1170.10448201034103999No Hit
AATCCAGGGATGGAGAGGCTGACTCTTGCTTGTGGTGGGGTAGCCCTGAATT1170.10448201034103999No Hit
ATTATGGGGATCAAATACATATTATGAATATGTGTTTCAATTTTCTGCATTT1170.10448201034103999No Hit
ACGGCAGGGGAGCCGCAGGAGGCACAGGCCTGTCCTGGGTCCCCGCAGGTCT1170.10448201034103999No Hit
CAGTATGGGATAATAGCTCTGCTGCAGTTCTGTGACTTCCAAGCCAGGAATT1170.10448201034103999No Hit
TACGCTGGGCTTAATTAAAGAAAAACTATTTAAAGGTAAAGGATATTTTGTT1170.10448201034103999No Hit
CAGCAAGGGCGTGGATCAAAACGAGGTCTTCCAGTTCTGCGGGTCAGAAGGC1170.10448201034103999No Hit
GTATGTGGGAGAGAATTTATGAATCACTGTTTCAAGAACCAGATTGGAAAGA1170.10448201034103999No Hit
AAACGAGGGCGCTGATTGCCATTTGCATTGTCTACTGAAAAGCTGTGGCAGC1160.1035890017056465No Hit
AACGTAGGGACTGCTCTCCTACAAGGAAGGAAAGATCTGCACGTGTAAAACA1160.1035890017056465No Hit
GTCCTAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1160.1035890017056465No Hit
CCTTGGGCCCTTACCGCCTTCCCCGCCACACCCGCTCTGTCTTCCCACTGTC1160.1035890017056465No Hit
TCTTAGGGTATCCCATGAGGCTAGGAGACAGGAGTCCTGGGTTTGAGCCCTG1160.1035890017056465No Hit
CTTCGGGGCACCCGTGGGTCTGTCAACGATCCACGGTGGCATCCATGATGCA1160.1035890017056465No Hit
CTCACCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1160.1035890017056465No Hit
TAGAAAGGGCAAGGAAATAGATTGGAAGCAAAAGAAGGAGGGAAAAGAGTAG1150.10269599307025298No Hit
TCTAGAGGGCGATCTTCAAGAGGGATCTTTTAACCAACTGAACAATACACCA1140.1018029844348595No Hit
TATACGCGGGCGTGAAGCCAGCAATTGTTTCGCAATTCGGCATTTTGAAAAC1140.1018029844348595No Hit
TCCACCGGGATATAATAACTATGATCAAAAAGCTATTTGGAATAGTTTGATT1140.1018029844348595No Hit
GAACGCGGGCCAGGATAGCTTTATGAATTGCTCTGACAGCATCATCAATGGT1140.1018029844348595No Hit
AGAACGGGATTGACTCATTTATGTCTTAGTCTTTTTTATTACAAATTATTTA1140.1018029844348595No Hit
GGTAGTGGGGTATAATATTGTGAATATTGGAAGTTTTGAGTGAGTGCTGTCT1140.1018029844348595No Hit
CGCTCAGGGGCAGCCTTGTGATGTTTGGAGCATAGGTTGCATTCTTATTGAA1140.1018029844348595No Hit
ATGAAAGGGATAGAAAAAAACATTCTATCTACTGATTTGGGCTGAATGTATG1130.10090997579946598No Hit
ACGCTGAGGGTAGATTGAAATGCTGTATTTCTCCAAAGTTAGGATGCTTAAA1130.10090997579946598No Hit
CTATTAGGGTTAAAGGCTAAACTCAAAATGGGATCTCCGAAGACTTTGTAAA1120.10001696716407248No Hit
ATCTAAAAAGTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAA1120.10001696716407248No Hit
GCACAGAGGGATATTCGGGTTTTCAGACCACCCAACTACTCTGGTACCATTG1120.10001696716407248No Hit
CAAGTAGGGGTCTTGCTACCAGAATCCATTCCTTTCTTAGCAGAGTTGATGG1120.10001696716407248No Hit
CTGAATGGGTGATTATTCCTACAAGTGAAACACTAGACTATTTGGAGTGTAT1120.10001696716407248No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)