FastQCFastQC Report
Sat 31 Dec 2022
SRR5849512.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849512.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127398
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT11670.9160269391984176RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10480.8226188794172593No Hit
GGCTTAGGGGACGCTCTTCCGATCTGCGCACGGGTAGAAGAAAAAAGATAAA2240.17582693605865085No Hit
GTAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2030.15934316080315233No Hit
CTTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1790.14050456051115404No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1790.14050456051115404No Hit
ATTATTGGGCAACTTGTATTGTGACGTTAGTACCAGGAATTCCCACTGGGGA1690.1326551437228214No Hit
CTTAGAGGGAGGGCAAGCGATAAGGAGGAAAAGAATCTCTAAAAGTACATCT1620.12716055197098855No Hit
CTGGCAGCGGATTACTTTTCTCTCTTTCTTCAACACTTGATTAAAACTTGCT1550.12166596021915571No Hit
CAATGTGGGACACTTGAGACTTCTGTTGTGAAATATTATGGCAGAGTGAAAA1510.11852619350382267No Hit
GCTTCAGGGAACTCATTCTCTTCTTGCTATATGATTTAGCAAGTTCTAGAAG1490.11695631014615614No Hit
TAAAATGGGGGAGGGGTGAGAAGTTGGCCCTGGTCCCGTCTGCCTGCTCCTC1470.11538642678848962No Hit
TAATACGGGTAGTAAGAAAATGTGCTCGAAAGTCTCAGAGACCCTATGATAT1470.11538642678848962No Hit
TTCTCTGGGACAGGTACCTAACTCCACTAATGTGTCCATGTGCTCTCTTGTA1460.11460148510965636No Hit
TTCAAAGGGGAAGAGCTTCCACATCTTAAGCAACAATATTAGGGTATTTGTG1400.10989183503665677No Hit
TACACAGGGAAAGGGATACTTCACAAATCTTAAAAATGTGCTATTTTTCAAA1390.1091068933578235No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCT1350.10596712664249046No Hit
AGTCTAGGGCTTCCTGAGTCTCTGTTTTTCTATTATTTAAAATTATTTGTTG1330.10439724328482394No Hit
CGAACCGGGGCTGGAGGCCGAGGACTACCCCCTGCTTCTAGGGTCGGCGGAC1320.10361230160599068No Hit
TCGGCTGGGGAAACTCCAGCAGCAACTTAAAATTACAACAGATAACAAGGTT1320.10361230160599068No Hit
CAATCAGGGGGGCCGTTTTATAAGATGTGGGTAGGTAAAGGAAAATTACAGT1320.10361230160599068No Hit
GTAGGCGGGCTACTGAAACTATTCCAAAAAATTGAGGAGGACAGACTCCTCC1310.10282735992715741No Hit
TTAGCTGGGGGAGGGTAGGAGAGCCTTGAGCCTGCGAGAAGAGTCTCTTAGA1310.10282735992715741No Hit
ACCAAAGGGGAAACAGCAACTGGTTAAGGTTTATGAAAAATATGGTTATCAT1310.10282735992715741No Hit
TTACAAGGGTGTTTCTCTAGTTAGACTAAAGAATCCCCACTATGAAGTTGTT1300.10204241824832415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)