FastQCFastQC Report
Sat 31 Dec 2022
SRR5849511.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849511.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129073
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT11460.8878696551563844RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10590.8204659378801142No Hit
GGCTTAGGGGACGCTCTTCCGATCTGCGCACGGGTAGAAGAAAAAAGATAAA2350.18206751218302822No Hit
GTAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2110.16347338327922958No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1850.14332974363344775No Hit
ATCGAGGGGAGCTCACTGCAACCTCCGCCTCTTGGGTTCAAGTAATTCTCCT1650.12783463621361554No Hit
CTTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1640.12705988084262393No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1590.1231861039876659No Hit
CAATGTGGGACACTTGAGACTTCTGTTGTGAAATATTATGGCAGAGTGAAAA1590.1231861039876659No Hit
ATTATTGGGCAACTTGTATTGTGACGTTAGTACCAGGAATTCCCACTGGGGA1580.1224113486166743No Hit
CTGGCAGCGGATTACTTTTCTCTCTTTCTTCAACACTTGATTAAAACTTGCT1520.11776281639072463No Hit
CTTAGAGGGAGGGCAAGCGATAAGGAGGAAAAGAATCTCTAAAAGTACATCT1520.11776281639072463No Hit
CGTCTTGGGGGTTGAGAGCTGGCACTAGAATTCTCAGAACTAAGCTCTGGAA1500.1162133056487414No Hit
GATTAAGGGCCAGCCCAGGTGGAACTCAGAGGAGTTTGCAATGGCCTCTGGC1490.1154385502777498No Hit
TATCAAGGGATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACTCTTGAC1490.1154385502777498No Hit
GCTTCAGGGAACTCATTCTCTTCTTGCTATATGATTTAGCAAGTTCTAGAAG1440.11156477342279177No Hit
ATAGCAGGGGTCACTCCTGAGATGCAGAGAGTCAAACGCAATCAAGAAAACA1440.11156477342279177No Hit
GAATAGGGGGCTGCCAAGTGTTCCTGAGAGGTGTTTAGGCACAGCAGAGGGG1440.11156477342279177No Hit
AGCCATGGGGAGGAATGGAGTCACATCACATGAATGGCTCTTTAGGCTCAGG1430.11079001805180014No Hit
GAAACGGGACTGTGATCATGATGAAAATGAATGAGGATGGCCTGACGCCTGA1420.11001526268080854No Hit
GTACCTGGGCCTGACCTGGTGACAGAGAGTGCCCTGCAGAAGTGAGTCTATT1410.10924050730981694No Hit
GCAAATGGGGGCCCGCCTTCGGGGCCCGGATCTCAAACAGTCGGGAAGAAGC1410.10924050730981694No Hit
TTCTCTGGGACAGGTACCTAACTCCACTAATGTGTCCATGTGCTCTCTTGTA1400.10846575193882532No Hit
ATAACTGGGATGGTGCCTTCCCTGATTCACACAGTTACAAATGATTACCCTT1400.10846575193882532No Hit
ATAAAGGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAG1390.10769099656783371No Hit
ATTATAGGGATGGGATAATGCTGCTTCCTTGGTCCAACTTTTTCATACAACA1370.10614148582585049No Hit
TCGGCTGGGGAAACTCCAGCAGCAACTTAAAATTACAACAGATAACAAGGTT1360.10536673045485886No Hit
GCGATAGGGGTGAGGAAGAGGCGTTTAAAATGCTCAAGTTTCTGATGTTTGA1340.10381721971287566No Hit
TAATACGGGTAGTAAGAAAATGTGCTCGAAAGTCTCAGAGACCCTATGATAT1330.10304246434188404No Hit
CTCCGCGGGATATATTTCTATGACATTATATCTTAGATGTGTAATTTATGAA1310.10149295359990083No Hit
TTCAAAGGGGAAGAGCTTCCACATCTTAAGCAACAATATTAGGGTATTTGTG1310.10149295359990083No Hit
TCCAGAGGGTCAGAAAAAAGGAAAGAAAGTCCTCTCTCCCCACTCCTTTCAA1300.10071819822890923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)