FastQCFastQC Report
Sat 31 Dec 2022
SRR5849510.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849510.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124776
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT11060.8863884080271847RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC10410.8342950567416811No Hit
GGCTTAGGGGACGCTCTTCCGATCTGCGCACGGGTAGAAGAAAAAAGATAAA2120.16990446880810411No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG1980.15868436237738026No Hit
GTAATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1860.14906712829390267No Hit
CTTAGAGGGAGGGCAAGCGATAAGGAGGAAAAGAATCTCTAAAAGTACATCT1580.12662691543245497No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCCTGTCTCTTATAC1580.12662691543245497No Hit
CTTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1450.11620824517535423No Hit
GCTAAAGGGAGAATTGAAATATCTATAAGACGAACTGGAGAAAGCATTAAGA1420.11380393665448482No Hit
ATCGAGGGGAGCTCACTGCAACCTCCGCCTCTTGGGTTCAAGTAATTCTCCT1400.11220106430723858No Hit
CGTCTTGGGGGTTGAGAGCTGGCACTAGAATTCTCAGAACTAAGCTCTGGAA1390.11139962813361544No Hit
CATACAGGGAGCTGTCACAGGTTTACTTGAACCAGAGAGACTAAAAAGTTTG1370.10979675578636917No Hit
TATCAAGGGATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACTCTTGAC1350.1081938834391229No Hit
CAATGTGGGACACTTGAGACTTCTGTTGTGAAATATTATGGCAGAGTGAAAA1340.10739244726549978No Hit
TCCAGAGGGTCAGAAAAAAGGAAAGAAAGTCCTCTCTCCCCACTCCTTTCAA1340.10739244726549978No Hit
ATTATTGGGCAACTTGTATTGTGACGTTAGTACCAGGAATTCCCACTGGGGA1330.10659101109187663No Hit
TTCAAAGGGGAAGAGCTTCCACATCTTAAGCAACAATATTAGGGTATTTGTG1330.10659101109187663No Hit
AGCTAGGGGCAGTGGTTTATTACATTATTGTATCCTATAAACATAAATTATT1330.10659101109187663No Hit
TTCTCTGGGACAGGTACCTAACTCCACTAATGTGTCCATGTGCTCTCTTGTA1320.10578957491825351No Hit
GAAACGGGACTGTGATCATGATGAAAATGAATGAGGATGGCCTGACGCCTGA1280.10258383022376098No Hit
ATTCGCGGGCTCTCTTTTCAATGGCAGTTGTCTTCTGATCTGTTGGCTTTAC1280.10258383022376098No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGA1270.10178239405013785No Hit
GGCATTGGGTGTAGTTGTTTTTCTTCATTTAAAATGGATCTGTTGGAGGTTA1260.1009809578765147No Hit
TTGCACGGGTGCTGTCTCAGGCACTACATGTCATTGATCTTGCCCCCAATCC1260.1009809578765147No Hit
GTGCATGGGCAAAGCTTCACCTCCAGCTTTTTACTGTCTCTCCCACCGCCAG1250.10017952170289159No Hit
CTCCATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.10017952170289159No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)