FastQCFastQC Report
Sat 31 Dec 2022
SRR5849509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179480
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT14680.8179184310229551RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC12930.720414530866949No Hit
GTTTTTGGGCAAATGTTTTTGAGCCTGAGTTTCCTTAAATCTTCTTCAGTTG2400.1337196344996657No Hit
TGTTCTGGGACCTTCATTTTTATTTTCTTTGCCTAGAATAACTATCATTCAA2270.1264764876309338No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG2250.1253621573434366No Hit
TCACAAGGGCACAACTTAATTGTAAAAGGAAGAAATCTGAAAGTTACTACAT2240.12480499219968799No Hit
GAGTGTGGGACTTTGTAGGAAGCCACTCTTCTCCCCTAAACAACTTCTGCCA2170.12090483619344773No Hit
GCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCC2150.11979050590595053No Hit
AATACAGGGTCATGGATTTATCTACCTTTGGTCTTTGATGTTGGTGGCCTTC2090.11644751504345888No Hit
ACACAACGGGCATTTCCCATATTTATCTCATCTGGTTAGCTGCCTCTGCTTC2050.11421885446846446No Hit
GGACATGGGGAGAGAAACCCTATACATGTAAAGAATGTGGCAAAGCCTTTAG2040.11366168932471585No Hit
TCAGAGGGGAATCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACA2030.11310452418096724No Hit
CGTGGTGTGGGAGAATATAACAGATTACATTTGTCTGTGTGAGAAAATAATG1980.1103186984622242No Hit
CGAGATGGGAATGTTTTGAAGTTATGTTTATGTTCTGATGTTATTAAAAACA1960.10920436817472699No Hit
TAAGTGGGGCGATCTAATACGGGGAATTAATGTTGGCAAATATGAGAACAAC1950.10864720303097837No Hit
CGCAGTGGGAGAACACACTGTTCCTTCTCTCTGGAATGTCCTTCCTAATCCG1940.10809003788722978No Hit
TTAACTGGGAATGGTTCCCTCCCACCCTTGTATCCCTCTCTTGTATGGTCTC1940.10809003788722978No Hit
TTGACAGGGAACTCTTAAAATTCCACAAGAAAACAAACAACCCAATTAAAAA1940.10809003788722978No Hit
GTCACAGGGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCTGAG1940.10809003788722978No Hit
GTAACAGGGGATCTGGCGCTGGGCATGAAGAAAAGCTTCTTTGATAAATGTG1930.10753287274348118No Hit
TAGTATGGGATAAACCTTGTGAGATAATTTGATTTTTAAAACTAGGTACTTC1930.10753287274348118No Hit
ACATATGGGGTTGGATTTTCTGCAGTTCTGATTCAGTTCTAAGAATTTGGTG1920.10697570759973256No Hit
TGCAAAGGGATCATGATTATTTTTATTTGGGAAATACCAAAAAATCATAAAT1920.10697570759973256No Hit
CTAAGTGGGGAAAAGAAAATGTGATAAATAGGAAACAAATATCAATAAGAAG1920.10697570759973256No Hit
CAAATCGGGCATAAATACCCTACGTACTGGCATATTTGAAACTCTTTTTCCA1910.10641854245598396No Hit
GCATGGGGGATTGTGGCTGAATTTTTGTGCCTATCTATAAAACACACTTTCA1910.10641854245598396No Hit
ATCTCTGGGGGGTCAAGGCAGCCATCTCGCCGTGAGACAGCAAGTGTCGGAT1910.10641854245598396No Hit
GTATACCGGGGAGACTTCGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAG1900.10586137731223534No Hit
GGTCCTGGGGTGGCTCGAGTGGGCTCTGCGGCTGCAGGACACACAGGACAAG1890.10530421216848673No Hit
GCTGTCGGGGGGGAATTGCACATTTTACCTTTTGGACAGTAATTTCTATCAC1870.10418988188098952No Hit
ATTTGTGGGGGTTTTGTTGTCATTGACACTATTCAGGGTGCATACGATAAGT1870.10418988188098952No Hit
AGAAGGGGAACAGAGAATATCTAGAAAGCATACATATTTCAGAGTAAACATG1860.10363271673724092No Hit
CTGCTCGGGAGGTGGAATGGCTATTTTAATATGCCCAGTGTGGATAAAATGT1860.10363271673724092No Hit
ATATCGGGCGGAGGAAAAGTTCGTTATGGACTGATCCCTGAGGAATTCTTCC1860.10363271673724092No Hit
AAGCTTGGGACTTTGACGATATTGAATTTCATATTTCATTTCTTCATCAACA1850.10307555159349233No Hit
TCGCATGGGAGAGGGAAACAATGTTTAGATAGGAAAAAGGAAGCCGTCTGTT1850.10307555159349233No Hit
TTGACTGGGATGTTGATGAGGCCTGAATACCGCAGCAAAATAGATGCCAAAT1850.10307555159349233No Hit
TACGAGAGGGGTTCCATGAAAATGTTTAGCATCCTTGCAGAATTACTTGAGA1850.10307555159349233No Hit
ATGTTCGGGAGTTACCAAGCTGAAGCACTTCCAGCTCCGGTAAGCGGGACTT1850.10307555159349233No Hit
CAAGCCGGTGGTCATTAAAGGACAGGAGCTAAGCTAGCAAAGCAAAACATCT1840.1025183864497437No Hit
AATTATGGGACTCAAAACCCTTGCTGATTTCCTTGAACCCAAGGATAATTTT1820.10140405616224649No Hit
TCTCATGGGCTAAAGATGAAGGGACTAGATTTAAATGACCTTGCTGACTTCC1800.10028972587474928No Hit
GGGCCCGGGTGGGGGTGGCAGGGCGGGACTGGGGCTCCTCCCAGGCTCGGGT1800.10028972587474928No Hit
GAATAAGGGGTCCTCTATTTTTAGTACAATAGAAAATTGGATGATGGCTCTT1800.10028972587474928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)