FastQCFastQC Report
Sat 31 Dec 2022
SRR5849508.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849508.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences180859
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT14180.7840361828827982RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC13030.7204507378676206No Hit
TGTTCTGGGACCTTCATTTTTATTTTCTTTGCCTAGAATAACTATCATTCAA2460.13601756064116247No Hit
GTTTTTGGGCAAATGTTTTTGAGCCTGAGTTTCCTTAAATCTTCTTCAGTTG2340.12938255768305695No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG2310.1277238069435306No Hit
GCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCC2310.1277238069435306No Hit
AAGCTTGGGACTTTGACGATATTGAATTTCATATTTCATTTCTTCATCAACA2230.12330047163812694No Hit
AATTATGGGACTCAAAACCCTTGCTGATTTCCTTGAACCCAAGGATAATTTT2180.12053588707224966No Hit
GTCACAGGGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCTGAG2170.1199829701590742No Hit
GCTGTCGGGGGGGAATTGCACATTTTACCTTTTGGACAGTAATTTCTATCAC2130.11777130250637236No Hit
TGCAAAGGGATCATGATTATTTTTATTTGGGAAATACCAAAAAATCATAAAT2130.11777130250637236No Hit
AATACAGGGTCATGGATTTATCTACCTTTGGTCTTTGATGTTGGTGGCCTTC2120.1172183855931969No Hit
TTTTCAGGGGGAGATACAAGGGAAGTGGCTATCGCCAGAGTCGGATTCGCCG2120.1172183855931969No Hit
TCAAGTGGGGTAATATGCAGACATTCATTAATCTTTGACTCACCTACTGATT2080.11500671794049508No Hit
ACATCAGGGACTACCTCGTTGCTTATGTAGGATAAGTTATTTAAACACAGTG2080.11500671794049508No Hit
TCAGAGGGGAATCAAGCAATTAATCCCAAGTTGCTTCAGTTGGTCGAAGACA2070.11445380102731963No Hit
TAAGTGGGGCGATCTAATACGGGGAATTAATGTTGGCAAATATGAGAACAAC2060.11390088411414416No Hit
TCTCATGGGCTAAAGATGAAGGGACTAGATTTAAATGACCTTGCTGACTTCC2040.11279505028779326No Hit
CGCAGTGGGAGAACACACTGTTCCTTCTCTCTGGAATGTCCTTCCTAATCCG2040.11279505028779326No Hit
TTGACTGGGATGTTGATGAGGCCTGAATACCGCAGCAAAATAGATGCCAAAT2030.1122421333746178No Hit
TACGAGAGGGGTTCCATGAAAATGTTTAGCATCCTTGCAGAATTACTTGAGA2030.1122421333746178No Hit
ATCTCTGGGGGGTCAAGGCAGCCATCTCGCCGTGAGACAGCAAGTGTCGGAT2020.11168921646144234No Hit
TAGTATGGGATAAACCTTGTGAGATAATTTGATTTTTAAAACTAGGTACTTC1990.11003046572191597No Hit
TCAAAGGGGACCCTCAGTAATGCTGCTGATTTACTCTCAGATGTCTCAAGTC1970.10892463189556506No Hit
CAAGCCGGTGGTCATTAAAGGACAGGAGCTAAGCTAGCAAAGCAAAACATCT1970.10892463189556506No Hit
ATATCGGGCGGAGGAAAAGTTCGTTATGGACTGATCCCTGAGGAATTCTTCC1960.1083717149823896No Hit
TGACTGGGGGTTTTTCAGCCTTCAGAAATGAAAGTTAAGGATTTTAGCACTA1960.1083717149823896No Hit
ACATATGGGGTTGGATTTTCTGCAGTTCTGATTCAGTTCTAAGAATTTGGTG1940.10726588115603868No Hit
GGTCCTGGGGTGGCTCGAGTGGGCTCTGCGGCTGCAGGACACACAGGACAAG1930.10671296424286322No Hit
TCTATTGGGGGCTCCGCTGAATCCGCCCGCGCGTCGCCGCCGTCGTCGCCGC1910.1056071304165123No Hit
GGACATGGGGAGAGAAACCCTATACATGTAAAGAATGTGGCAAAGCCTTTAG1910.1056071304165123No Hit
TCGCATGGGAGAGGGAAACAATGTTTAGATAGGAAAAAGGAAGCCGTCTGTT1900.10505421350333687No Hit
GCTCCTGGGGTTGTCATTGGTAAATCTGTCACTGACTTAAGCGAGGTATAAA1890.1045012965901614No Hit
TTGACAGGGAACTCTTAAAATTCCACAAGAAAACAAACAACCCAATTAAAAA1880.10394837967698595No Hit
GCATTTGGGCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGA1880.10394837967698595No Hit
AACCTTGGGTATTTTATATAGGATGTAATTGGAAAATCGATGAATCAAGCAA1870.10339546276381048No Hit
AGAAGGGGAACAGAGAATATCTAGAAAGCATACATATTTCAGAGTAAACATG1870.10339546276381048No Hit
AATATGGGGGACCCGTCGACATCCGCCCCCACGTGACCCAAACAGATCCGGC1860.10284254585063501No Hit
GTAACAGGGGATCTGGCGCTGGGCATGAAGAAAAGCTTCTTTGATAAATGTG1850.10228962893745956No Hit
GAGTGTGGGACTTTGTAGGAAGCCACTCTTCTCCCCTAAACAACTTCTGCCA1840.10173671202428412No Hit
ATATCGGGGATGTAAGAGAAAGGGAAAAATAGATAAAAATGGATGTCATGGG1840.10173671202428412No Hit
TAGTGCCGGGCAGCAAGTAAACATTCCTCTGCTCACCTCCCAACAAGACTAA1840.10173671202428412No Hit
CTAAGTGGGGAAAAGAAAATGTGATAAATAGGAAACAAATATCAATAAGAAG1840.10173671202428412No Hit
CGAGATGGGAATGTTTTGAAGTTATGTTTATGTTCTGATGTTATTAAAAACA1840.10173671202428412No Hit
TTAACTGGGAATGGTTCCCTCCCACCCTTGTATCCCTCTCTTGTATGGTCTC1830.10118379511110866No Hit
GCATGGGGGATTGTGGCTGAATTTTTGTGCCTATCTATAAAACACACTTTCA1830.10118379511110866No Hit
TGATAGGGGATGCATGCCAAATCAAAGAAGCATCGTGTCAGAAACAGGAGAA1820.1006308781979332No Hit
TATTGGGGGTATTGGGTATGTAAATTCTAAGTATTTGGCAAAAATGCTTGCT1810.10007796128475774No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)