FastQCFastQC Report
Sat 31 Dec 2022
SRR5849507.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849507.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175870
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGCCGTCT13900.7903565133337124RNA PCR Primer, Index 23 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTGATCTCGTATGC12860.7312219252857224No Hit
TGTTCTGGGACCTTCATTTTTATTTTCTTTGCCTAGAATAACTATCATTCAA2300.13077841587536249No Hit
GTCACAGGGTATTCTTCACGGCAGCCAGAAGTTAATGGTGGTAGCAGCTGAG2280.12964121225905498No Hit
GTTTTTGGGCAAATGTTTTTGAGCCTGAGTTTCCTTAAATCTTCTTCAGTTG2180.12395519417751748No Hit
TAGTATGGGATAAACCTTGTGAGATAATTTGATTTTTAAAACTAGGTACTTC2170.12338659236936374No Hit
GGGCCCGGGTGGGGGTGGCAGGGCGGGACTGGGGCTCCTCCCAGGCTCGGGT2070.11770057428782624No Hit
GCCCGCCTTGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCC2070.11770057428782624No Hit
AATTATGGGACTCAAAACCCTTGCTGATTTCCTTGAACCCAAGGATAATTTT2030.11542616705521122No Hit
TTGACAGGGAACTCTTAAAATTCCACAAGAAAACAAACAACCCAATTAAAAA2030.11542616705521122No Hit
TAAGTAGGGTATCAAAGTTTTTCATTTAATGGGAGAATCTAAGAGTTTGTGT2020.1148575652470575No Hit
TTAAACGGGATAGTATATATATTTCAAGCTGAATGTTCAGAAAGAATAAGGT2020.1148575652470575No Hit
GTCAAGGGTACTATGAAGAGTCATAAATACTGTGTGAGTTCAGGAGACATAG2020.1148575652470575No Hit
ACATATGGGGTTGGATTTTCTGCAGTTCTGATTCAGTTCTAAGAATTTGGTG2010.11428896343890374No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCTAGTTG2010.11428896343890374No Hit
TGATAGGGGATGCATGCCAAATCAAAGAAGCATCGTGTCAGAAACAGGAGAA2010.11428896343890374No Hit
ATATCGGGCGGAGGAAAAGTTCGTTATGGACTGATCCCTGAGGAATTCTTCC1950.11087735258998124No Hit
TTCGCTGGGAAGTGAAAAAGAAATGACTATATACAATCAATGCTATTTATTG1940.11030875078182749No Hit
TACGAGAGGGGTTCCATGAAAATGTTTAGCATCCTTGCAGAATTACTTGAGA1930.10974014897367373No Hit
TCACAAGGGCACAACTTAATTGTAAAAGGAAGAAATCTGAAAGTTACTACAT1920.10917154716552No Hit
TAGTGCCGGGCAGCAAGTAAACATTCCTCTGCTCACCTCCCAACAAGACTAA1920.10917154716552No Hit
GCATTTGGGCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGA1890.10746574174105873No Hit
ACACAACGGGCATTTCCCATATTTATCTCATCTGGTTAGCTGCCTCTGCTTC1880.10689713993290498No Hit
TTGACTGGGATGTTGATGAGGCCTGAATACCGCAGCAAAATAGATGCCAAAT1880.10689713993290498No Hit
ATTTGTGGGGGTTTTGTTGTCATTGACACTATTCAGGGTGCATACGATAAGT1870.10632853812475122No Hit
CGTGGTGTGGGAGAATATAACAGATTACATTTGTCTGTGTGAGAAAATAATG1860.1057599363165975No Hit
GGTCTGGGGAAGACAGATTCAAAATCATATCTTCATTCCTTGTATTTAACAG1850.10519133450844374No Hit
AAGCTTGGGACTTTGACGATATTGAATTTCATATTTCATTTCTTCATCAACA1840.10462273270028999No Hit
TAAATAGGGATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATA1840.10462273270028999No Hit
TCTCATGGGCTAAAGATGAAGGGACTAGATTTAAATGACCTTGCTGACTTCC1820.10348552908398248No Hit
TCGCATGGGAGAGGGAAACAATGTTTAGATAGGAAAAAGGAAGCCGTCTGTT1810.10291692727582874No Hit
AGAAGGGGAACAGAGAATATCTAGAAAGCATACATATTTCAGAGTAAACATG1810.10291692727582874No Hit
CTAAGTGGGGAAAAGAAAATGTGATAAATAGGAAACAAATATCAATAAGAAG1810.10291692727582874No Hit
CAAATCGGGCATAAATACCCTACGTACTGGCATATTTGAAACTCTTTTTCCA1790.10177972365952123No Hit
TCTTACGGGAGGTGAATTGCTTCAGATTTTCTCTAGTACCAGAGGGTACCTC1790.10177972365952123No Hit
GCTAACGGGAATCAAGACAAACTGGGATTTGAGGATGGATCAGTTCTGAAAC1790.10177972365952123No Hit
CTTTGCGGGCTTGTGGTGGGTGGTAAGTTCTCACGAGATTTATTTATCTGAG1790.10177972365952123No Hit
TGATGAGGAGCACAACATGGTGACACAGTTAATAACAATGTATTGTAAACTT1790.10177972365952123No Hit
TATCTCGGGAACTCAAACATTGTAGACTAGCTTTGTGTTTATTCTTCAGGTC1790.10177972365952123No Hit
TTTGCAGGGGAATGTCAGGAGACACACAACCCTGCTAACTGGCCTCTTAAAA1780.10121112185136748No Hit
GCATGGGGGATTGTGGCTGAATTTTTGTGCCTATCTATAAAACACACTTTCA1780.10121112185136748No Hit
TGCAAAGGGATCATGATTATTTTTATTTGGGAAATACCAAAAAATCATAAAT1770.10064252004321372No Hit
ATCCTGGGGGTCCGCGCGCCGTCAGCGGCGCGGGTGGGGCATTGCCAGCTCG1770.10064252004321372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)