FastQCFastQC Report
Sat 31 Dec 2022
SRR5849500.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849500.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108729
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT11611.0677924012912838Illumina Paired End PCR Primer 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC10290.9463896476560991No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1750.16095062034967672No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1330.1223224714657543No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1150.10576755051550184No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)