FastQCFastQC Report
Sat 31 Dec 2022
SRR5849498.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849498.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107519
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT11011.0240050595708665Illumina Paired End PCR Primer 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC10310.9589002873910658No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1730.16090179410150762No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1390.1292794761856044No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1310.12183893079362718No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)