FastQCFastQC Report
Sat 31 Dec 2022
SRR5849491.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849491.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94544
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC9871.043958368590286No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT8500.8990522931122017Illumina Paired End PCR Primer 2 (95% over 21bp)
ACAACAGGGAAAAGCAGCCAGCATGTTGCTTAATGGGAAAACACTAAAAAAA1600.16923337282112033No Hit
AACCTAGGGATCTCAACCCTTGAGTATATGACTTTTTAATAGCCTCTGTGAG1490.1575985784396683No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1480.1565408698595363No Hit
AACTAGGGGTATTTAAAATTGAATATTTTATGTTGACTTCTTTCATACTAGC1470.15548316127940429No Hit
GTGCTTGGGGGTCAGTGATTGATGACCTCGATATGGATTTAGATTATCAAAT1430.1512523269588763No Hit
ATGAATGGGGGGCTCAAGAGGTCTGGATCTGTGGTGAGATGGGGGAAGTGGG1420.1501946183787443No Hit
GGATTTGGGAGAAGCTTGATTTGAATGAGAACTTTTATATGAAACTCTTCTC1350.14279065831782028No Hit
AAAGTGGGGAGGGAAAAGTTAGTTTTTAAATAACCACTTGTACATACTACCC1330.14067524115755628No Hit
TTAGCGGGGAGCCCCATAAGATCTTTAAAAAGCCTCCAATCATTTAAAGGAA1330.14067524115755628No Hit
TTGAGAGGGGCAATAAGACTGTTTTACTTTCTTATCATTGGTGTGTTCACTG1330.14067524115755628No Hit
GTTCTTGGGCATTGTTTTTGTTTTTGTTTTTTGTTTTTTTGTGTGAATATGC1330.14067524115755628No Hit
AACCGTGGAAACCCAATTTGCATTGTGAACCCTAGCTGCAAGGGAGTCTGAG1290.13644440683702824No Hit
AATTAGGGGCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT1280.13538669825689625No Hit
GCTTACGGGCAGAGACTGAAGCAGTTGGTGGAAAGGTGTCTCCTCGAGCTGA1280.13538669825689625No Hit
AATTCTGGGTCATCATGTTGGCAAGGCTGGTTTCGAACTCCTGACCTTGTGA1280.13538669825689625No Hit
CGCAACGGGTCTGGAATCCAGTCCCAAGCTCTGAAGACCAAGATGATATTAA1270.13432898967676427No Hit
AGTATCGGGCAGAACTCTGCCTGGAGCACAGAGTCTCCAAGGACGGCAACGG1270.13432898967676427No Hit
ACTGTTGGGGGCCCGGAGGACAGAGCGGCCCGGTCGCCGGCATGGTTTCTCC1260.13327128109663225No Hit
AGTCCAGGGACGGTATTCAGACCTGGACTTTGAGGTGATGCACACCCGGAAA1250.13221357251650026No Hit
GCAGCTGGGGAGCCTCCGATGCTCTGTGAAATGGAAGGAATGTGCTTGCCTG1230.13009815535623626No Hit
CTAAAGCGGGAGGTTTGTGCCATAAAGTATTTTTTCAAAGACACCAAGATGT1230.13009815535623626No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1220.12904044677610427No Hit
TCTGTTAGGGCTTAGTTTTATAGTCATAAATATGTACATTAAAATTACCAAT1210.12798273819597225No Hit
AAAGTAGGAGGGAGATTTTCTCTATCTTTATTTAAAATAACCAAGAAAAGCT1200.12692502961584023No Hit
AAAGGTGGGTACTGACTTACTAGGAAGTTGATAGTGAAATGTGAAGGGTTCA1190.12586732103570822No Hit
AGCGCAGGGACATCACAGAATTCATACTGGAGAGAAACCTTATGAGTGCAGT1180.12480961245557624No Hit
TACGCATGGGGTACAAAACAGGCCCTCCTTTCAGGAACTGGTCTCGAGAGAG1180.12480961245557624No Hit
CGACTAGGGAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGGCA1180.12480961245557624No Hit
CGAAATGGGCCACAAAGCTGTTCTTCAGAAATGAAGGAGAGGTAAATACTTT1180.12480961245557624No Hit
AATTAGGGGGTGTGTGTGTTTATGTAATGAATGTCATCAGTTTAAAATAATG1170.12375190387544424No Hit
GTTAATGGGCAGGCAACTCATCAGCCAGATTGGTAATGCTATGGGCTATGTA1170.12375190387544424No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1170.12375190387544424No Hit
GGATCTGGGCTGGTTTTAAGGCAGTAATTTAATTATAAAACGTTTAGGAGGT1160.12269419529531223No Hit
TTATTAGGGAAGAGCAAATGGGTCTGAATGAAGATGGTTTATTTTTCACTAA1140.12057877813504825No Hit
CGAAATGGGACTTAAGAATAAACTCAGTCAATGAGGAAAAAGACTTGTATAC1140.12057877813504825No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1140.12057877813504825No Hit
GCATGGGGATCCTTCTCTTTCAATGTGTTTAATTTTGGAGTAACGTTTTCCT1140.12057877813504825No Hit
ACACTTGGGCTGAAGCAAATTCTGTGGTCAGGAGGATGAAGTGTTGGGATTG1140.12057877813504825No Hit
TCCAGCGGGAGTGCATTCCTTTGATGAGAAAACAAGGAAGATTTCCTTTCGT1130.11952106955491623No Hit
CCGCAATGGGAATTATCCTTATGAATAAATACAATGGATTCTATACCTTTTG1130.11952106955491623No Hit
CGTAGCGGGCTTAGGATTAAGGGTCCAAAAATGCTGATCTAAGGGGTTGCCA1120.11846336097478423No Hit
TATGTAGGGGTTCCAAAAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGT1110.11740565239465221No Hit
TGGAATGGGATACAGATTAAATGAAAGAAAATATTAGAATACAGTACCTGGT1110.11740565239465221No Hit
TTATATGGGGGGTATATCCATCATTTAGAAGTCAATATGTATTACCATAGAG1110.11740565239465221No Hit
CTTTGTGGGGGAAAAGAACTGATGAAGTGCAGAGTCTCTCCAGACAATGGTA1110.11740565239465221No Hit
AGCATTGGGGACACACAAACGCTGCCCAGGATTACCCGCCAGCTCACGCCGC1110.11740565239465221No Hit
GCGATAGGGATGGTTGACTAAAAGCAAAGATGATTAAGTTTCCATGTCTGCA1100.11634794381452022No Hit
GAACTCGGGCTTTCTAAATTCTTTGCTAGTCATTTCTTCCTGGGAATACAGA1100.11634794381452022No Hit
TATCTAGGGGAAAATGTTAAATTGAGAAACCTGTTAACTTACATTTTATGAA1100.11634794381452022No Hit
CATTGTGGGATACCATATTAATACAATGAAGAGGGGAAAACCCATGTGATCA1100.11634794381452022No Hit
AACCCTGGGGCATAACCAAATCAATACTAGAGTATCGGATTTACAGCTTGAC1100.11634794381452022No Hit
AATTCAGGGTCCAGAGTTGATTTTCAAATGAAAGAGCTATAAGTTATATCTA1090.11529023523438822No Hit
GTTCCCGGGAGTCTAAAAAGTAAAAGGGGCGATCAGGTCCATCTCAAAGGTC1090.11529023523438822No Hit
AAACATGGGAGCTCTGGGAATGGAATGTGACCCTTGTGGAGAGCTATAAATG1080.11423252665425622No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1080.11423252665425622No Hit
AAAAAAGGGGAGAAGCTCCGCTCTAAATACAGATGCAACTCCATTTGAAAAG1070.11317481807412422No Hit
CTAAAGGGTACAAAGCAAGGATGTAGAATTGACCTCTATGAAAGGGGAGGTT1070.11317481807412422No Hit
ACAATAGGGAACGCGGCACATTTTCTGCAGGTCAGCAGTAAGGTTTCAAGGT1070.11317481807412422No Hit
TACGACGGGGGTTACTCTTCCCAGTGATACCTAGATTTTAAGAGTAGAGAAC1060.11211710949399223No Hit
TATACGGGGATTTTAGCAAATTGAATAGTAGACCAAGTGGGATTTATTCCTA1060.11211710949399223No Hit
TAGTGCGGGCATGGTGACAAGATCTATGCTTCTGCTTGAGTTGGACAGTAGA1060.11211710949399223No Hit
ACTGGTGGGAGCCTAATATATTTTGATTGAAAGTCCTTATGACATCATAATT1060.11211710949399223No Hit
TAGCTCGGGCGATCTGTCTGCGGGTCTTGCCGACTGAACAGTGCTCTTAGGA1060.11211710949399223No Hit
AACGCGGGGAACTCGGTTTAATGGAGTTGAGCTTATGGTGTAAGATCTTGCA1060.11211710949399223No Hit
TTCATAGGGACCCATGTAAACCACATGTTAGATAAATTTTTGCAAGTAGAGG1050.11105940091386021No Hit
ATATGGGTGAGTAATCCACTGAATATGCTTTTGCCTTTACTACAAACAGTTT1050.11105940091386021No Hit
ACATACGGGGTTTTAAGTAAAGAATTGGGCCATTTTAGTTACCTTAGTTAAC1050.11105940091386021No Hit
TGTAAAGGGGTTTGACTACTTAATTGTCATTGATTTTGAATCGACATGCTGG1050.11105940091386021No Hit
ATGTTAGGGGACACTTGGAATAAGTATTTTGTACCGCAAGCCCAATGGTACA1050.11105940091386021No Hit
CCATATGGGGCCCATTTTATGGAGGAGAACGCTGGGGCACAGAGACGTTTAG1050.11105940091386021No Hit
CACTTTGGGAATTGAAGAAAATAAAATATGTGTATGCACCTTATCTTTCAAA1040.11000169233372822No Hit
TAAATTGGGGGCATCGGCCCTCACTGCCTGTATCTTACTGTCCTGTTTTGTT1040.11000169233372822No Hit
CATACCGGGGAAATGATGACATGAAATGATCAGATGAGATGAGATGAGATGA1040.11000169233372822No Hit
GCGGCTGGGACCATCTCTTCATACCAGCACATTAGTGACTAGGTTTCAATAT1040.11000169233372822No Hit
CTTTCCGGGATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTTCT1040.11000169233372822No Hit
GTAGTTGGGGGGGTACTTTGACAGTTAATTTCAGATTTCCTAGATGAACTGA1030.10894398375359621No Hit
ACTGCGGGCCGATCTAAGACTGGGTCAGGTGGTCCCCAACCATAAGCTAGGT1030.10894398375359621No Hit
TCTATTGGGGGAGCAGTTCTAATAATGCAGAATAAATACATGTTGCCTTAAA1030.10894398375359621No Hit
TGGAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1030.10894398375359621No Hit
CACTTTGGGGTCATTGCCGTTCTTTTCCTTACACGCAAAGATTTTTTAACTA1030.10894398375359621No Hit
AGTTGTGGGATTGTGATTGTGTTTATTGATAGAGTGGATGTGGTGTTGACAT1020.1078862751734642No Hit
GCAACAGGGCCATTGACTCTCAAATATTCTTATGAGTTATTTATACTAAATG1010.10682856659333222No Hit
AAACAGTGGGGGAAAACAAAAGAAAGTGCCAGAGAGAGATGAGAATGGTGTA1000.1057708580132002No Hit
TTTCTGGGGATAGTAGCCTGGTAAAAGACAAATTCTGTGTTATCTAGAGAAT1000.1057708580132002No Hit
ATTAACGGGACTTCCCCTATAGTACAATAAATAATTATTTAAAAGTCATAGC1000.1057708580132002No Hit
GCTAAGGGCTGTTAATGTTAATCCCAATCACCTGGCTTAAGGTAGTGTTTGC1000.1057708580132002No Hit
AGGTAGGGGGATCACTTCTTTTTTAAGGAATACACATTGATCTTATTTCACC990.1047131494330682No Hit
GTGCCAGGGAATTTTAACATTTAAGCACGTTATATACAGAAAATCAGCAAGC990.1047131494330682No Hit
GCATAAAGGGAGACTTTAACCTTTAATTTTAATTGCTAGTTTACTTTTATGT990.1047131494330682No Hit
GCTTATGGGAGGCATAGCCTGGACTCAACAATTCTGTTCTGTGTATGTGTTT980.1036554408529362No Hit
GTTATTGGGAACTCATTTGTTTCCCTAGTCTCAGTTATAGTATTGTATTTTT980.1036554408529362No Hit
CAACTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC970.10259773227280419No Hit
TGCACAGGGGCGGGTCCCCACATGCACACAGAACTCTGACACAGGATCAGCT970.10259773227280419No Hit
GTGTCTGGGTCAGAACAGCATTGACGACAAACTGTTGGTAGAGAAAATCTTT970.10259773227280419No Hit
GCTATCGGGAGGCATACTTTTTTTAAAAAAAGAGTGTCTATTCTTTAATGAG970.10259773227280419No Hit
TGTACCGGGGCAAACAGGAATTAAAAAGCTGGAGTGGCTATTTCACAAAATA970.10259773227280419No Hit
ATAACTGGGGAAGGACTGGAGCAAGGTCGTCCTGGCCTATGAGCCTGTGTGG970.10259773227280419No Hit
AAAGCTGGGCAGGATTGCCTTCTGTGTGACTTTTTCTTGCAGGATCTGACAT970.10259773227280419No Hit
TGATGCGGGATTCGACTGGGAGATGTTCTTGTAAGGTAAGAAACAAATAAAT960.1015400236926722No Hit
CCAAATGGGAGTGTCAGTTTTTATGCTTCACTAAATGATAAGGCCTCTGTTT960.1015400236926722No Hit
TACACAGGGCATTCTATTAAACAGCACATGGAACTTTCTCCAAGCTAGACCA960.1015400236926722No Hit
CAGCATGGGACATTACTGTAATGGTGCTGGTTGAATCACTGTATTATTATAG960.1015400236926722No Hit
TTCGCAGGGTTACCACCCATATGGAAACATTTGATGTGATAAATCATGCATT960.1015400236926722No Hit
TTAGCAGGGATTATCTTTGGAGTTATAGGGCCACAGGGTTTTAAAAGGAGGT960.1015400236926722No Hit
TATACAGGGATCGTTGCATTTGTTTTAATTTGTGGAAAAGTATTGTATCTAA960.1015400236926722No Hit
TTAAATGGGGAGAGAGTTGACTTCCGGCGGCTTGTGGGAGTGCTGGTTCTGT960.1015400236926722No Hit
AAATGGGGGACCTCCCACAAGGTCCCTCCTACGACTTGTGGGGATTATGCAA960.1015400236926722No Hit
ATCCTTGGGGATTGTAGAATGGTATTGAATTGGTCTGTGGAAAATTGCATTG950.10048231511254019No Hit
TTAAGTGGGGATCTTATACCGGGGTTTATAAATTACCAGTCTAAGATATTTT950.10048231511254019No Hit
TCGTGAGGGAAGTGAATGATTCAGATAATAAATGTAGAAATCAAGACCTATT950.10048231511254019No Hit
TAAATTGGGGTTTCTGCAAGCTGTATCCCAAGTGCACTGTCCTTCTGTCCTG950.10048231511254019No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)