FastQCFastQC Report
Sat 31 Dec 2022
SRR5849490.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849490.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93898
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC9721.0351658182282901No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT8910.9489020000425994Illumina Paired End PCR Primer 2 (95% over 21bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1580.16826769473258216No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1510.16081279686468297No Hit
TATCTAGGGGAAAATGTTAAATTGAGAAACCTGTTAACTTACATTTTATGAA1490.1586828260452832No Hit
GCAGCTGGGGAGCCTCCGATGCTCTGTGAAATGGAAGGAATGTGCTTGCCTG1420.15122792817738398No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1410.15016294276768408No Hit
ACTGTTGGGGGCCCGGAGGACAGAGCGGCCCGGTCGCCGGCATGGTTTCTCC1410.15016294276768408No Hit
TTGAGAGGGGCAATAAGACTGTTTTACTTTCTTATCATTGGTGTGTTCACTG1410.15016294276768408No Hit
AGTATCGGGCAGAACTCTGCCTGGAGCACAGAGTCTCCAAGGACGGCAACGG1400.1490979573579842No Hit
AGTCCAGGGACGGTATTCAGACCTGGACTTTGAGGTGATGCACACCCGGAAA1370.14590300112888455No Hit
GCTTACGGGCAGAGACTGAAGCAGTTGGTGGAAAGGTGTCTCCTCGAGCTGA1350.14377303030948474No Hit
AGCGCAGGGACATCACAGAATTCATACTGGAGAGAAACCTTATGAGTGCAGT1330.141643059490085No Hit
AATTAGGGGGTGTGTGTGTTTATGTAATGAATGTCATCAGTTTAAAATAATG1330.141643059490085No Hit
AACCGTGGAAACCCAATTTGCATTGTGAACCCTAGCTGCAAGGGAGTCTGAG1310.1395130886706852No Hit
ACAATAGGGAACGCGGCACATTTTCTGCAGGTCAGCAGTAAGGTTTCAAGGT1310.1395130886706852No Hit
AAAGTGGGGAGGGAAAAGTTAGTTTTTAAATAACCACTTGTACATACTACCC1310.1395130886706852No Hit
TAGCTCGGGCGATCTGTCTGCGGGTCTTGCCGACTGAACAGTGCTCTTAGGA1300.13844810326098533No Hit
GCTATAGGGCAGCACGGGAAGTTCCAAACAGCTGACTTACAGATAAGCATAT1280.13631813244158555No Hit
GTTCTTGGGCATTGTTTTTGTTTTTGTTTTTTGTTTTTTTGTGTGAATATGC1280.13631813244158555No Hit
GCATGGGGATCCTTCTCTTTCAATGTGTTTAATTTTGGAGTAACGTTTTCCT1270.13525314703188568No Hit
ACAACAGGGAAAAGCAGCCAGCATGTTGCTTAATGGGAAAACACTAAAAAAA1270.13525314703188568No Hit
CTAAAGCGGGAGGTTTGTGCCATAAAGTATTTTTTCAAAGACACCAAGATGT1260.13418816162218578No Hit
GTGCTTGGGGGTCAGTGATTGATGACCTCGATATGGATTTAGATTATCAAAT1260.13418816162218578No Hit
TCTGTTAGGGCTTAGTTTTATAGTCATAAATATGTACATTAAAATTACCAAT1250.1331231762124859No Hit
ATGAATGGGGGGCTCAAGAGGTCTGGATCTGTGGTGAGATGGGGGAAGTGGG1250.1331231762124859No Hit
CGAAATGGGCCACAAAGCTGTTCTTCAGAAATGAAGGAGAGGTAAATACTTT1250.1331231762124859No Hit
GGATCTGGGCTGGTTTTAAGGCAGTAATTTAATTATAAAACGTTTAGGAGGT1250.1331231762124859No Hit
GCTAAGGGCTGTTAATGTTAATCCCAATCACCTGGCTTAAGGTAGTGTTTGC1240.132058190802786No Hit
AACTAGGGGTATTTAAAATTGAATATTTTATGTTGACTTCTTTCATACTAGC1240.132058190802786No Hit
AATTCTGGGTCATCATGTTGGCAAGGCTGGTTTCGAACTCCTGACCTTGTGA1240.132058190802786No Hit
CCGCAATGGGAATTATCCTTATGAATAAATACAATGGATTCTATACCTTTTG1220.12992821998338622No Hit
CGTATAGGGAACTTAATAAGACAATTGCTAATGATAAGACCAGGTAATTTGT1220.12992821998338622No Hit
CGACTAGGGAGCCTCAGCTCCAGGCTATCTGGCAATAACAAAGAAAGTGGCA1220.12992821998338622No Hit
AATTAGGGGCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT1210.12886323457368634No Hit
GCGATAGGGATGGTTGACTAAAAGCAAAGATGATTAAGTTTCCATGTCTGCA1200.12779824916398644No Hit
AGCATTGGGGACACACAAACGCTGCCCAGGATTACCCGCCAGCTCACGCCGC1190.12673326375428656No Hit
TTAGCGGGGAGCCCCATAAGATCTTTAAAAAGCCTCCAATCATTTAAAGGAA1190.12673326375428656No Hit
GGATTTGGGAGAAGCTTGATTTGAATGAGAACTTTTATATGAAACTCTTCTC1190.12673326375428656No Hit
AACCTAGGGATCTCAACCCTTGAGTATATGACTTTTTAATAGCCTCTGTGAG1170.12460329293488678No Hit
GTTCCCGGGAGTCTAAAAAGTAAAAGGGGCGATCAGGTCCATCTCAAAGGTC1170.12460329293488678No Hit
TCGTTAGGGATTGGGTTCATACATAATCAGAGGCAATATCGCCATTTCAGGG1160.12353830752518691No Hit
CATTGTGGGATACCATATTAATACAATGAAGAGGGGAAAACCCATGTGATCA1150.12247332211548702No Hit
TACGACGGGGGTTACTCTTCCCAGTGATACCTAGATTTTAAGAGTAGAGAAC1140.12140833670578711No Hit
TCGCACTGGGGTGAAATCTACAAACCAAAACAAGAACTTTTAAATCTGGGAT1140.12140833670578711No Hit
ATATGGGTGAGTAATCCACTGAATATGCTTTTGCCTTTACTACAAACAGTTT1140.12140833670578711No Hit
AAAGTAGGAGGGAGATTTTCTCTATCTTTATTTAAAATAACCAAGAAAAGCT1130.12034335129608724No Hit
TACGCATGGGGTACAAAACAGGCCCTCCTTTCAGGAACTGGTCTCGAGAGAG1130.12034335129608724No Hit
TGGAATGGGATACAGATTAAATGAAAGAAAATATTAGAATACAGTACCTGGT1110.11821338047668746No Hit
ATGACAGGGTACAAGCCAATATCAGCTTCCCATTGTGTAATAACTTGGGTGT1110.11821338047668746No Hit
TCATAAGGGGTTCTAGCAAGTCTAAGGAGAATACTATACAATGACCTTTTCT1110.11821338047668746No Hit
ATGTTAGGGGACACTTGGAATAAGTATTTTGTACCGCAAGCCCAATGGTACA1110.11821338047668746No Hit
TAGTGCGGGCATGGTGACAAGATCTATGCTTCTGCTTGAGTTGGACAGTAGA1110.11821338047668746No Hit
TGTGGCGGGCCATCAAATGTCTATGACCCTCAGAAAGCATGATGGTTAACAA1110.11821338047668746No Hit
CGTAGCGGGCTTAGGATTAAGGGTCCAAAAATGCTGATCTAAGGGGTTGCCA1110.11821338047668746No Hit
AAAGGTGGGTACTGACTTACTAGGAAGTTGATAGTGAAATGTGAAGGGTTCA1100.11714839506698758No Hit
GTTATTGGGAACTCATTTGTTTCCCTAGTCTCAGTTATAGTATTGTATTTTT1090.1160834096572877No Hit
AACCCTGGGGCATAACCAAATCAATACTAGAGTATCGGATTTACAGCTTGAC1090.1160834096572877No Hit
ACATACGGGGTTTTAAGTAAAGAATTGGGCCATTTTAGTTACCTTAGTTAAC1080.11501842424758779No Hit
AGTTGTGGGATTGTGATTGTGTTTATTGATAGAGTGGATGTGGTGTTGACAT1080.11501842424758779No Hit
CTTTGTGGGGGAAAAGAACTGATGAAGTGCAGAGTCTCTCCAGACAATGGTA1080.11501842424758779No Hit
TTACTTGGGATCTGAAGCGTCTCTGAAATCTACCCTTGCAAGCTTCAGACAA1070.11395343883788792No Hit
CGCAACGGGTCTGGAATCCAGTCCCAAGCTCTGAAGACCAAGATGATATTAA1060.11288845342818804No Hit
AAACATGGGAGCTCTGGGAATGGAATGTGACCCTTGTGGAGAGCTATAAATG1060.11288845342818804No Hit
GCTATCGGGAGGCATACTTTTTTTAAAAAAAGAGTGTCTATTCTTTAATGAG1060.11288845342818804No Hit
GCTCAAGGGACACGTTTCTGTTATTAAAGTATTTGATTACTAATTCAAATCA1060.11288845342818804No Hit
ATTAACGGGACTTCCCCTATAGTACAATAAATAATTATTTAAAAGTCATAGC1060.11288845342818804No Hit
AATTCAGGGTCCAGAGTTGATTTTCAAATGAAAGAGCTATAAGTTATATCTA1060.11288845342818804No Hit
TTCATAGGGACCCATGTAAACCACATGTTAGATAAATTTTTGCAAGTAGAGG1050.11182346801848814No Hit
TCCAGCGGGAGTGCATTCCTTTGATGAGAAAACAAGGAAGATTTCCTTTCGT1050.11182346801848814No Hit
GTGTCTGGGTCAGAACAGCATTGACGACAAACTGTTGGTAGAGAAAATCTTT1050.11182346801848814No Hit
TATACTGGGAAGATCATTTTGTTGATATCCCTTTTTACTCTATGAACATTAC1050.11182346801848814No Hit
TCGAACGGGTCCAATTCTGATGGGTATGTCATTCATTTATTCATTCATTCAT1050.11182346801848814No Hit
CTAAAGGGTACAAAGCAAGGATGTAGAATTGACCTCTATGAAAGGGGAGGTT1040.11075848260878826No Hit
TCGTGAGGGAAGTGAATGATTCAGATAATAAATGTAGAAATCAAGACCTATT1040.11075848260878826No Hit
TTAAATGGGGAGAGAGTTGACTTCCGGCGGCTTGTGGGAGTGCTGGTTCTGT1040.11075848260878826No Hit
AACGTCGGGATAGTAGGAGGTATGTGCCATCACTGAAGCATGTCTGCCTTTT1040.11075848260878826No Hit
TATGTAGGGGTTCCAAAAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGT1030.10969349719908837No Hit
TACCATGGGAGAGTAATTAGAGACAGAGTACAACCATCTCATGTGAGACGTT1030.10969349719908837No Hit
CGTAACGGGAGGTGGAAATCAAGATATTAAAAAACGTGTATATACTACCATG1030.10969349719908837No Hit
TGTACCGGGGCAAACAGGAATTAAAAAGCTGGAGTGGCTATTTCACAAAATA1030.10969349719908837No Hit
TTATATGGGGGGTATATCCATCATTTAGAAGTCAATATGTATTACCATAGAG1030.10969349719908837No Hit
TACGCGAGGAAAGAGAAGTATTATTGCATTGTAGGTTTTTGCATTGATGTGT1030.10969349719908837No Hit
TTCGCAGGGTTACCACCCATATGGAAACATTTGATGTGATAAATCATGCATT1030.10969349719908837No Hit
TTATTAGGGAAGAGCAAATGGGTCTGAATGAAGATGGTTTATTTTTCACTAA1020.1086285117893885No Hit
GCGGCTGGGACCATCTCTTCATACCAGCACATTAGTGACTAGGTTTCAATAT1020.1086285117893885No Hit
CTCACTGGGCGGCTCACTGCAACCTCCATCTCTTGGGTTCAAGCAATTCTCC1010.10756352637968859No Hit
CGAAATGGGACTTAAGAATAAACTCAGTCAATGAGGAAAAAGACTTGTATAC1010.10756352637968859No Hit
CTTCCGGGATAAATAAAGTATTTATTTTATGCCTCAGTATATTATTATTTAA1010.10756352637968859No Hit
AAATGTGGGGAACGTCTGAAATGGAGGCCCAGGGCTGCCACGATGGCTCTGT1000.10649854096998872No Hit
AAAAAAGGGGAGAAGCTCCGCTCTAAATACAGATGCAACTCCATTTGAAAAG1000.10649854096998872No Hit
AAACAGTGGGGGAAAACAAAAGAAAGTGCCAGAGAGAGATGAGAATGGTGTA1000.10649854096998872No Hit
GTAGTTGGGGGGGTACTTTGACAGTTAATTTCAGATTTCCTAGATGAACTGA1000.10649854096998872No Hit
CATTACGGGAGGGCATAGTTTGCAAATATTTTCTCCCATTCTGTAGGATGTC1000.10649854096998872No Hit
CTAAGTGGGAATTAAAATGTTTTGATACCTATAGGAGCTAGAAATCTGTGCT1000.10649854096998872No Hit
GGGCCTGGGGTCAAGACCTTTGTAATTGAGGGTGCCTTGGCTGGGTCCAGGA1000.10649854096998872No Hit
CAAATTGGGCCGATCTACCTAGGGGATTGGCATTCCTTGACTAAAAAGGACC990.10543355556028883No Hit
GTTAATGGGCAGGCAACTCATCAGCCAGATTGGTAATGCTATGGGCTATGTA990.10543355556028883No Hit
CACTTTGGGAATTGAAGAAAATAAAATATGTGTATGCACCTTATCTTTCAAA990.10543355556028883No Hit
TGATGCGGGATTCGACTGGGAGATGTTCTTGTAAGGTAAGAAACAAATAAAT990.10543355556028883No Hit
GTTCAAGGGAGGACCACCCTTTATTCTTAAATTTGTGTGTGTCCAACAGTTG990.10543355556028883No Hit
GTAACTGGGAGAGTAAAAGCTAATAGCATTTTTGTTATACCAAAACTGAAAG990.10543355556028883No Hit
CCATATGGGGCCCATTTTATGGAGGAGAACGCTGGGGCACAGAGACGTTTAG990.10543355556028883No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA990.10543355556028883No Hit
AAAGCTGGGCAGGATTGCCTTCTGTGTGACTTTTTCTTGCAGGATCTGACAT990.10543355556028883No Hit
ACGACGGGGCTTCCGATCTGCCAAGGGGAGAGCTTTTGGGGAAGGAAAGTAT980.10436857015058894No Hit
TTAAGTGGGGATCTTATACCGGGGTTTATAAATTACCAGTCTAAGATATTTT980.10436857015058894No Hit
CTATCTAGGGATATGGAGTACATGCACTTGGGCATTTAATATATACCACTGG980.10436857015058894No Hit
CCACTTGGGGCCTGGTTGATGTTCTTAAAGGAAACGAATTATTAAAACACTA980.10436857015058894No Hit
TGTAAAGGGGTTTGACTACTTAATTGTCATTGATTTTGAATCGACATGCTGG970.10330358474088905No Hit
TATACGGGGATTTTAGCAAATTGAATAGTAGACCAAGTGGGATTTATTCCTA970.10330358474088905No Hit
GCTGCTGGTGAGACTGGGTTTCTGTTTACACATTTACTGGCCATTTGGATTT970.10330358474088905No Hit
CCATATGGGATTATTCCTCATGCATCATTTGCCATTTAACTGTATTTTTTTC970.10330358474088905No Hit
TGCATAGGGCTCCATCAGACACTCCATGAAAGGTCACAATTTCTCTTGATAT970.10330358474088905No Hit
TTACGTGGGTGAACACATATGTCCACCCCAAAATTTGTACGTAAATGTTCAC960.10223859933118917No Hit
GGGTTAGGGGCCATTGGCTAAGTCATCTTGCACACAGCAGATGTTTCTCTTT950.10117361392148927No Hit
TCTCCAGGGGTTCACTGTTGGCATATAGATATGCTACTGATATTGATTGATT950.10117361392148927No Hit
AAATGGGGGACCTCCCACAAGGTCCCTCCTACGACTTGTGGGGATTATGCAA950.10117361392148927No Hit
CGGTCTGGGGGAAATCTTAAATGAAAAACGCATCTCATTGTAGGCATTTTTG940.1001086285117894No Hit
AATATTGGGAAGATTTTTCTTCTGTAGATCAATCTCCAAGTTCAGCAAGGAG940.1001086285117894No Hit
CATCTAGGGCAAATGCAGATTGCATATTGTTATATATATAGTACTTTGTGTT940.1001086285117894No Hit
ACTGGTGGGAGCCTAATATATTTTGATTGAAAGTCCTTATGACATCATAATT940.1001086285117894No Hit
GGGCTCCTTCTGGCCTTCAGATATGTCATGGGTATTGCTGGACTTAGGTTCT940.1001086285117894No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)