FastQCFastQC Report
Sat 31 Dec 2022
SRR5849489.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849489.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94320
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC9340.9902459711620017No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT8340.8842239185750635Illumina Paired End PCR Primer 2 (95% over 21bp)
CTAAAGCGGGAGGTTTGTGCCATAAAGTATTTTTTCAAAGACACCAAGATGT1590.16857506361323157No Hit
GCAGCTGGGGAGCCTCCGATGCTCTGTGAAATGGAAGGAATGTGCTTGCCTG1440.15267175572519084No Hit
AACCTAGGGATCTCAACCCTTGAGTATATGACTTTTTAATAGCCTCTGTGAG1400.1484308736217133No Hit
ATGAATGGGGGGCTCAAGAGGTCTGGATCTGTGGTGAGATGGGGGAAGTGGG1380.14631043256997456No Hit
GTGCTTGGGGGTCAGTGATTGATGACCTCGATATGGATTTAGATTATCAAAT1340.14206955046649702No Hit
AGTCCAGGGACGGTATTCAGACCTGGACTTTGAGGTGATGCACACCCGGAAA1320.13994910941475827No Hit
TCGTGAGGGAAGTGAATGATTCAGATAATAAATGTAGAAATCAAGACCTATT1310.1388888888888889No Hit
AACCCTGGGGCATAACCAAATCAATACTAGAGTATCGGATTTACAGCTTGAC1310.1388888888888889No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1300.1378286683630195No Hit
ACAACAGGGAAAAGCAGCCAGCATGTTGCTTAATGGGAAAACACTAAAAAAA1300.1378286683630195No Hit
GCGATAGGGATGGTTGACTAAAAGCAAAGATGATTAAGTTTCCATGTCTGCA1290.13676844783715014No Hit
CGTAACGGGAGGTGGAAATCAAGATATTAAAAAACGTGTATATACTACCATG1290.13676844783715014No Hit
GCATGGGGATCCTTCTCTTTCAATGTGTTTAATTTTGGAGTAACGTTTTCCT1280.13570822731128074No Hit
TCTGTTAGGGCTTAGTTTTATAGTCATAAATATGTACATTAAAATTACCAAT1270.1346480067854114No Hit
CCATATGGGGCCCATTTTATGGAGGAGAACGCTGGGGCACAGAGACGTTTAG1270.1346480067854114No Hit
GGATTTGGGAGAAGCTTGATTTGAATGAGAACTTTTATATGAAACTCTTCTC1270.1346480067854114No Hit
AGCGCAGGGACATCACAGAATTCATACTGGAGAGAAACCTTATGAGTGCAGT1250.1325275657336726No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1240.13146734520780323No Hit
AAAGTGGGGAGGGAAAAGTTAGTTTTTAAATAACCACTTGTACATACTACCC1220.12934690415606448No Hit
TTACGTGGGTGAACACATATGTCCACCCCAAAATTTGTACGTAAATGTTCAC1220.12934690415606448No Hit
TATGTAGGGGTTCCAAAAGCTGAGGTCATTTTTGCAAGTAAGGAAATACAGT1210.12828668363019508No Hit
ACTGTTGGGGGCCCGGAGGACAGAGCGGCCCGGTCGCCGGCATGGTTTCTCC1210.12828668363019508No Hit
CATTGTGGGATACCATATTAATACAATGAAGAGGGGAAAACCCATGTGATCA1210.12828668363019508No Hit
AATTAGGGGGTGTGTGTGTTTATGTAATGAATGTCATCAGTTTAAAATAATG1200.1272264631043257No Hit
GTTCCCGGGAGTCTAAAAAGTAAAAGGGGCGATCAGGTCCATCTCAAAGGTC1200.1272264631043257No Hit
GGATCTGGGCTGGTTTTAAGGCAGTAATTTAATTATAAAACGTTTAGGAGGT1200.1272264631043257No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1190.12616624257845632No Hit
TTATATGGGGGGTATATCCATCATTTAGAAGTCAATATGTATTACCATAGAG1180.12510602205258692No Hit
AATTCTGGGTCATCATGTTGGCAAGGCTGGTTTCGAACTCCTGACCTTGTGA1180.12510602205258692No Hit
CCGCAATGGGAATTATCCTTATGAATAAATACAATGGATTCTATACCTTTTG1180.12510602205258692No Hit
TGGAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1180.12510602205258692No Hit
TTGAGAGGGGCAATAAGACTGTTTTACTTTCTTATCATTGGTGTGTTCACTG1180.12510602205258692No Hit
TCCAGCGGGAGTGCATTCCTTTGATGAGAAAACAAGGAAGATTTCCTTTCGT1170.12404580152671756No Hit
AATTAGGGGCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCT1150.1219253604749788No Hit
CACTTTGGGAATTGAAGAAAATAAAATATGTGTATGCACCTTATCTTTCAAA1150.1219253604749788No Hit
ATGTTAGGGGACACTTGGAATAAGTATTTTGTACCGCAAGCCCAATGGTACA1150.1219253604749788No Hit
AACTAGGGGTATTTAAAATTGAATATTTTATGTTGACTTCTTTCATACTAGC1150.1219253604749788No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1150.1219253604749788No Hit
AGGTAGGGGGATCACTTCTTTTTTAAGGAATACACATTGATCTTATTTCACC1140.12086513994910941No Hit
GTTAATGGGCAGGCAACTCATCAGCCAGATTGGTAATGCTATGGGCTATGTA1140.12086513994910941No Hit
GTTCTTGGGCATTGTTTTTGTTTTTGTTTTTTGTTTTTTTGTGTGAATATGC1140.12086513994910941No Hit
CTAAAGGGTACAAAGCAAGGATGTAGAATTGACCTCTATGAAAGGGGAGGTT1120.11874469889737065No Hit
TAGTGCGGGCATGGTGACAAGATCTATGCTTCTGCTTGAGTTGGACAGTAGA1120.11874469889737065No Hit
AAATGGGGGACCTCCCACAAGGTCCCTCCTACGACTTGTGGGGATTATGCAA1120.11874469889737065No Hit
GTAGTTGGGGGGGTACTTTGACAGTTAATTTCAGATTTCCTAGATGAACTGA1110.11768447837150126No Hit
TACGCATGGGGTACAAAACAGGCCCTCCTTTCAGGAACTGGTCTCGAGAGAG1110.11768447837150126No Hit
CTATCTAGGGATATGGAGTACATGCACTTGGGCATTTAATATATACCACTGG1110.11768447837150126No Hit
TCGTTAGGGATTGGGTTCATACATAATCAGAGGCAATATCGCCATTTCAGGG1110.11768447837150126No Hit
CGAAATGGGCCACAAAGCTGTTCTTCAGAAATGAAGGAGAGGTAAATACTTT1110.11768447837150126No Hit
ACAATAGGGAACGCGGCACATTTTCTGCAGGTCAGCAGTAAGGTTTCAAGGT1100.11662425784563189No Hit
AAAGGTGGGTACTGACTTACTAGGAAGTTGATAGTGAAATGTGAAGGGTTCA1100.11662425784563189No Hit
CGTAGCGGGCTTAGGATTAAGGGTCCAAAAATGCTGATCTAAGGGGTTGCCA1100.11662425784563189No Hit
TGGAATGGGATACAGATTAAATGAAAGAAAATATTAGAATACAGTACCTGGT1090.1155640373197625No Hit
ATATGGGTGAGTAATCCACTGAATATGCTTTTGCCTTTACTACAAACAGTTT1090.1155640373197625No Hit
TATACAGGGATCGTTGCATTTGTTTTAATTTGTGGAAAAGTATTGTATCTAA1090.1155640373197625No Hit
CGTATAGGGAACTTAATAAGACAATTGCTAATGATAAGACCAGGTAATTTGT1090.1155640373197625No Hit
CGCAACGGGTCTGGAATCCAGTCCCAAGCTCTGAAGACCAAGATGATATTAA1080.11450381679389314No Hit
AAAGTAGGAGGGAGATTTTCTCTATCTTTATTTAAAATAACCAAGAAAAGCT1080.11450381679389314No Hit
TGTACCGGGGCAAACAGGAATTAAAAAGCTGGAGTGGCTATTTCACAAAATA1080.11450381679389314No Hit
TTAGCGGGGAGCCCCATAAGATCTTTAAAAAGCCTCCAATCATTTAAAGGAA1080.11450381679389314No Hit
AAAAAAGGGGAGAAGCTCCGCTCTAAATACAGATGCAACTCCATTTGAAAAG1060.11238337574215437No Hit
AACCGTGGAAACCCAATTTGCATTGTGAACCCTAGCTGCAAGGGAGTCTGAG1060.11238337574215437No Hit
GGGCTCGGGGATTGTTTTTATTTTATGGTTACATATGATGATAACCTGCTAT1060.11238337574215437No Hit
GCTTATGGGAGGCATAGCCTGGACTCAACAATTCTGTTCTGTGTATGTGTTT1060.11238337574215437No Hit
GCTTACGGGCAGAGACTGAAGCAGTTGGTGGAAAGGTGTCTCCTCGAGCTGA1060.11238337574215437No Hit
TATGAGGGGGTTCTGCAAACAAATGAGCATATTAGTGTAACATAGTATCAAT1060.11238337574215437No Hit
TAAATTGGGGTTTCTGCAAGCTGTATCCCAAGTGCACTGTCCTTCTGTCCTG1060.11238337574215437No Hit
GAACTCGGGCTTTCTAAATTCTTTGCTAGTCATTTCTTCCTGGGAATACAGA1060.11238337574215437No Hit
TATCTAGGGGAAAATGTTAAATTGAGAAACCTGTTAACTTACATTTTATGAA1060.11238337574215437No Hit
AATAAAGGGGGGTGCTTGGCTTTAAGTACAGTTCTTAGATTCAGCTCCTCTA1060.11238337574215437No Hit
AACGTCGGGATAGTAGGAGGTATGTGCCATCACTGAAGCATGTCTGCCTTTT1060.11238337574215437No Hit
TATACTGGGAAGATCATTTTGTTGATATCCCTTTTTACTCTATGAACATTAC1050.11132315521628498No Hit
GCTTGGCGGGAATGAAGAGAGTTGAGTTTGTAGACTCAGTTATTGGGTACAA1050.11132315521628498No Hit
ACACTTGGGCTGAAGCAAATTCTGTGGTCAGGAGGATGAAGTGTTGGGATTG1050.11132315521628498No Hit
TACGACGGGGGTTACTCTTCCCAGTGATACCTAGATTTTAAGAGTAGAGAAC1040.11026293469041562No Hit
TGCACAGGGGCGGGTCCCCACATGCACACAGAACTCTGACACAGGATCAGCT1040.11026293469041562No Hit
ACTTTGGGGATTCTTGCCGTATTCTCATGATCCCAGATAAGTGTGTGTTTTA1040.11026293469041562No Hit
TACTTGGGCGACAGTGCAACCTTCAGTCTGTGGTCGAAGGTCCAAGAGTCCA1030.10920271416454623No Hit
CTTTGTGGGGGAAAAGAACTGATGAAGTGCAGAGTCTCTCCAGACAATGGTA1030.10920271416454623No Hit
TCGAACGGGTCCAATTCTGATGGGTATGTCATTCATTTATTCATTCATTCAT1030.10920271416454623No Hit
GCAACAGGGCCATTGACTCTCAAATATTCTTATGAGTTATTTATACTAAATG1030.10920271416454623No Hit
TTCATAGGGACCCATGTAAACCACATGTTAGATAAATTTTTGCAAGTAGAGG1020.10814249363867684No Hit
TGTTCTGGGATAGAAAAATAATTTTAAAAATTTGAATGGAAGGGGGTAAAGG1020.10814249363867684No Hit
TTAAATGGGGAGAGAGTTGACTTCCGGCGGCTTGTGGGAGTGCTGGTTCTGT1020.10814249363867684No Hit
GTTTAGGGGACCGCTTCCTAAACCATTTTGCAGAAATGTAAGGGTGTTCGGT1020.10814249363867684No Hit
ATATGTGGGCGATCTTAGCACCGGGAGGAGGTGGAATCACTCATATTTTGTT1010.10708227311280746No Hit
CTTATAGGGAGCATCTAAAAGGTAATCATATGTTTCATTGGTTATTTTTTTC1010.10708227311280746No Hit
CGAAATGGGACTTAAGAATAAACTCAGTCAATGAGGAAAAAGACTTGTATAC1010.10708227311280746No Hit
AGTATCGGGCAGAACTCTGCCTGGAGCACAGAGTCTCCAAGGACGGCAACGG1000.10602205258693807No Hit
TACACAGGGCATTCTATTAAACAGCACATGGAACTTTCTCCAAGCTAGACCA1000.10602205258693807No Hit
GCACAGGGAATCCATTGACTGTGGAGAGAACTCTCCAGAAGTTTTAAATTTC1000.10602205258693807No Hit
ATACTGGGATTCCTGTCCCAAATCAAAGAGCATGGTTCTCAGGAAAACCAGT990.10496183206106871No Hit
GGCAGGTAAAGTAGAGGGGCCAGGTAGAGATCCTGCTGACCTGGCAAGCACA990.10496183206106871No Hit
TGCGCTGGGGGAATTTATATATACCTGATTTCAGCCTTCCAGCTTACTCAGT990.10496183206106871No Hit
GCTATCGGGAGGCATACTTTTTTTAAAAAAAGAGTGTCTATTCTTTAATGAG990.10496183206106871No Hit
GCTGCTGGTGAGACTGGGTTTCTGTTTACACATTTACTGGCCATTTGGATTT990.10496183206106871No Hit
TTCGCAGGGTTACCACCCATATGGAAACATTTGATGTGATAAATCATGCATT990.10496183206106871No Hit
GACCTAGGGGTAGTAGATGCTTAAAGTGGAAAATAGTGTTATGATGACTTAT990.10496183206106871No Hit
AACGCGGGGAACTCGGTTTAATGGAGTTGAGCTTATGGTGTAAGATCTTGCA990.10496183206106871No Hit
ATGTCTGGGAGGACTTGTTCATTAGGTTGGCAGCAGAGGGGCAGAAGGAATT980.10390161153519932No Hit
TAGTCGGGGGCTGACTAGATTAGAATGAAATGTAAGATATTACATAAATTAG980.10390161153519932No Hit
TTAAGTGGGGATCTTATACCGGGGTTTATAAATTACCAGTCTAAGATATTTT970.10284139100932994No Hit
TCATAAGGGGTTCTAGCAAGTCTAAGGAGAATACTATACAATGACCTTTTCT970.10284139100932994No Hit
AAGAATGGGCTCACAAACTTCTTTACGAAGAGTGAAATATGTTTTTGTACCT970.10284139100932994No Hit
GAATTAGGGGAGACTGGCTTTGTGGCCTAGCATATGGTCTATTTGGGAGAAA970.10284139100932994No Hit
AGCATTGGGGACACACAAACGCTGCCCAGGATTACCCGCCAGCTCACGCCGC970.10284139100932994No Hit
TTACCAGGGAGCTAACATAATCATGCCTCTTATTCTGCTGTCATGATATCAC970.10284139100932994No Hit
AAACAGTGGGGGAAAACAAAAGAAAGTGCCAGAGAGAGATGAGAATGGTGTA960.10178117048346055No Hit
CATTGAGGGCGATCTTGCGGCGGGGAGGAAAAATAATTTTCTGTTTTTGGAA960.10178117048346055No Hit
GCTAAGGGCTGTTAATGTTAATCCCAATCACCTGGCTTAAGGTAGTGTTTGC960.10178117048346055No Hit
TCTATTGGGGGAGCAGTTCTAATAATGCAGAATAAATACATGTTGCCTTAAA960.10178117048346055No Hit
ATAACTGGGGAAGGACTGGAGCAAGGTCGTCCTGGCCTATGAGCCTGTGTGG960.10178117048346055No Hit
AACTGTGGGAACCAAAAGTTTGTACCTTTTTACTAACATTTCCCCATTGATC960.10178117048346055No Hit
TTGTGTGGGACAACACTACAAGTCTTGAGTTAAGAAGGAAAGAGGAAAAAAA960.10178117048346055No Hit
AAATGTGGGGAACGTCTGAAATGGAGGCCCAGGGCTGCCACGATGGCTCTGT950.10072094995759116No Hit
CCAAAAGGGACTCCAATTTCTTTATTTTCTATGTAAATTGTGTTGATGGATC950.10072094995759116No Hit
AGTCACGGGATCTGGAAGGATTACATCTACCCTAATATACATATGCATTCAA950.10072094995759116No Hit
TCTGTGTGGGCATACCAATCTTTCCCTTTATAGTTGCTACTTTTGCTATCAT950.10072094995759116No Hit
GTGTCTGGGTCAGAACAGCATTGACGACAAACTGTTGGTAGAGAAAATCTTT950.10072094995759116No Hit
GTTCAAGGGAGGACCACCCTTTATTCTTAAATTTGTGTGTGTCCAACAGTTG950.10072094995759116No Hit
GTGCCAGGGAATTTTAACATTTAAGCACGTTATATACAGAAAATCAGCAAGC950.10072094995759116No Hit
TACGCGAGGAAAGAGAAGTATTATTGCATTGTAGGTTTTTGCATTGATGTGT950.10072094995759116No Hit
CTTTCCGGGATCCTAGCTGACAAATTATTGATTAATAAGAACTTGAATTTCT950.10072094995759116No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)