FastQCFastQC Report
Sat 31 Dec 2022
SRR5849485.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849485.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65572
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC5950.9073994997864941No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT3600.5490148234002318Illumina Paired End PCR Primer 2 (95% over 21bp)
ATACGCGGGAAGATTAAATCCGGCATACAGAATACAGCCATGCTTCTCAAAG970.14792899408284024No Hit
TTATTTGGGGAGGGGGCGCGGGGACACAGCCAGGCGCCCCTGCCCGCCGCGG880.13420362349783446No Hit
ATCAGAGGGATCCTTTGTCACTTGCCCTCTGGCTTGGGCTCCTTTTTCTGGC820.1250533764411639No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC820.1250533764411639No Hit
ATCATGGGGAGTGAGGCTGAAAGGGGGGGCTATGGAAGAGCGGTAGGGAGTC780.11895321173671689No Hit
TCAATGGGGGTGATGGACAGTAATGACCGGGAGCGGGTCCAAGAATCTGCTG770.11742817056060513No Hit
ACACTGGGTACTTCTTGTGCTTTACTCTGAATTTCCAGTGTGGTCTTGTTTA770.11742817056060513No Hit
AGCCATGGGATGGCATCCATCGTGTCTGAGATCATGATGTATGTGCTCATTG760.11590312938449339No Hit
ACACAGAGAGAGAAGTTAACAGATGAAGAAGTTGATGAAATGATCAGGGAAG760.11590312938449339No Hit
AAGCATGGGATACATATATTTTACAACCTGACACACATACACACATGCACAT760.11590312938449339No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT750.11437808820838162No Hit
GAAGCTGGGGCCTCTGGAGCTTTTTCCCTTTATAGCACCATTGAATCCCAGT740.11285304703226988No Hit
CATAGGGGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGC740.11285304703226988No Hit
CTTGGAGGCTTGAGGAAACTGAAACAGCTTGTCATTTTCTCTGTTTTGTTTT740.11285304703226988No Hit
TTTGCTAGGGAGTGTGGACTGGAGCCGAGATGGAGGCCTCATTTGTACCTCC740.11285304703226988No Hit
TTTACTGGGACAGACCACCTTTCACAAAACGAAATGCTATGACTGTATCTTG730.11132800585615811No Hit
ATTGTCGGGATGGCCAAGTGTTAAAATCTGTGTTTGGAAAATAGCGAATTAA730.11132800585615811No Hit
AATCGCGGGATCCTTCCTTTTAAATTTATATAGATCTAATGAACATTGCCAA730.11132800585615811No Hit
GGTAAGGGTTGCCATAAGAAACTGCTGAGTACTTGGCAAACCCTTTCATAGT730.11132800585615811No Hit
GCAACTGGGAAGAATGTCAGTATACAAGAAGGAATAGAAAACTGATACTGTT720.10980296468004636No Hit
GGCGCAGGGAAGAATGGGGACGGCACCATTGACTTCCGAGAGTTCATCTGCG720.10980296468004636No Hit
AAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAA720.10980296468004636No Hit
AAGTATGGGATCAAAGAAAAGGGCAGCAACCTAAAGGAAAAATGGATGTAAA720.10980296468004636No Hit
GGACAAGGGCACGTGTAAAAATTCATCAGAAGAGCTGTGAAAGGCAAAAGGC720.10980296468004636No Hit
TCATATGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGCTAAC710.10827792350393461No Hit
CTAATCACGGGGTTCCCGACCCGGAGACGCGGGGCCACAGCCCCCAGCTGAC710.10827792350393461No Hit
GAAAGTGGGGAAAACAAAAAGCCAGAATCTTCCTTTTTTTGTTTCATTGGGG700.10675288232782285No Hit
ACAACAGGGGCCGGCGTCGGGCGGGGCCGGCGGGGTCTTCAGGGTACCGGGC700.10675288232782285No Hit
CATCGCCGGGAGATGTATGCATATATTCCACATTTTGAAAAAGTCAAATACA690.1052278411517111No Hit
ACTTGGGAGGCTGAGGCAGAAGAATGGCTCAGGAGGTGGAGGTTGCAGTGAG690.1052278411517111No Hit
ACAGTAGGGGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTC690.1052278411517111No Hit
ACACCTGGGGGCGACGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTC680.10370279997559934No Hit
GGTAGGGGGGAGAAGGCCATTGAGAAGGTGGATGACCTGCTGGAGAGTTACA670.10217775879948758No Hit
GTAGTTGGGAGGGAGCCCCAAGGGGGATTTTGTGGGACCGCTGCCCACAGAT670.10217775879948758No Hit
ATATGTGGGCCTGAAGCTTCAACTTCATGTCGAAGATTCACTGGGACCCAAT660.10065271762337584No Hit
GTGGCCGGGAGAGATCTCAAACTCCGATGCCCCTGGGGCCACGTATGTTGTG660.10065271762337584No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)