FastQCFastQC Report
Sat 31 Dec 2022
SRR5849484.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849484.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65568
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC5990.9135553928745729No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT3690.5627745241581259Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC900.13726207906295754No Hit
CTGCGAGGGATCATATGTAACATTTACTCCTGTTTCTGCTGATTGTTTTTTT850.12963640800390436No Hit
TTTGCTAGGGAGTGTGGACTGGAGCCGAGATGGAGGCCTCATTTGTACCTCC840.1281112737920937No Hit
GAAGCTGGGGCCTCTGGAGCTTTTTCCCTTTATAGCACCATTGAATCCCAGT820.12506100536847242No Hit
CTGGTGGGGGGTAACCGGTAGCGCTTGCAGCATGGCTGACCAACTGACTGAA820.12506100536847242No Hit
TATAAAGGGGGGGCCAAGGCGGCTGTTCTCGCTCCAGCTCGATGCTGCCTCC790.12048560273304051No Hit
CAAGCTGGGCGATCTCTGTGTGGGAACCTGTTGCATCATCAAAAGTGTACCT780.11896046852122988No Hit
ATACGCGGGAAGATTAAATCCGGCATACAGAATACAGCCATGCTTCTCAAAG780.11896046852122988No Hit
TGGTATGGGAGTGCTTAATATGTGTAAGGACTTCCTCTTTAAATTTGGTACC770.11743533430941923No Hit
TGTCGCGGGAGGGTCCTCAACTGTAAGATAGGAAGGGTGTCTGACCTCTAAG770.11743533430941923No Hit
GGCGCAGGGAAGAATGGGGACGGCACCATTGACTTCCGAGAGTTCATCTGCG750.11438506588579796No Hit
TTATTTGGGGAGGGGGCGCGGGGACACAGCCAGGCGCCCCTGCCCGCCGCGG750.11438506588579796No Hit
TCATATGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGCTAAC740.11285993167398731No Hit
CTTGGAGGCTTGAGGAAACTGAAACAGCTTGTCATTTTCTCTGTTTTGTTTT740.11285993167398731No Hit
CGCTACGGCAGATTTTACCATGGAGAACTTTTTTTTTAGTTTTTACCTTTTC730.11133479746217667No Hit
TAGTTTGGGCTTTGCTGTTTTGTCAGAACTCAGTAGCAGAACTTTTCTGATA730.11133479746217667No Hit
ACACCTGGGGGCGACGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTC730.11133479746217667No Hit
CTAGTTGGGTATTTTTGTGATTTACTTTTTCTGTAAGCCTTTTGGGGTTTAC720.10980966325036604No Hit
AAGTATGGGATCAAAGAAAAGGGCAGCAACCTAAAGGAAAAATGGATGTAAA720.10980966325036604No Hit
AATCGCGGGATCCTTCCTTTTAAATTTATATAGATCTAATGAACATTGCCAA710.1082845290385554No Hit
CATAGGGGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGC700.10675939482674476No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT700.10675939482674476No Hit
GGTAAGGGTTGCCATAAGAAACTGCTGAGTACTTGGCAAACCCTTTCATAGT700.10675939482674476No Hit
TATCGAGGGAGACTAGAGGCAAGCATTTAGATCAGGAGGCAAAGTAATTTCT690.10523426061493411No Hit
GGACCAGGGTCTTCAGACGGGACTGGTTCATAGAATATGTGCATATTCACCT680.10370912640312348No Hit
TCAGCAGGGGAAGTGTGCCTCTCACAGATGGAAGGTGCACACGCTCCTGTCT680.10370912640312348No Hit
ACAGTAGGGGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTC680.10370912640312348No Hit
GCAACTGGGAAGAATGTCAGTATACAAGAAGGAATAGAAAACTGATACTGTT670.10218399219131284No Hit
AGCCATGGGATGGCATCCATCGTGTCTGAGATCATGATGTATGTGCTCATTG660.10065885797950219No Hit
TTTGATGGGCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGATCAG660.10065885797950219No Hit
ACATAGGGGACACTTTAAAGGCAACAGGTCATACAGTTCTTTAAATCTGATC660.10065885797950219No Hit
CTCAAGGGGAGGCAATAAATAAATGGGCACAGCTGTGTTCCAATAATACTTT660.10065885797950219No Hit
ACTTGGGAGGCTGAGGCAGAAGAATGGCTCAGGAGGTGGAGGTTGCAGTGAG660.10065885797950219No Hit
CGTACCGGGGCCAGGCGCCCGTCCAGGGACACACACAGTCTTCACTGTGCTG660.10065885797950219No Hit
CTTCAAGGGGAAACTCCTGCTGGGGAAGGACTTCCCTGCCTCCCCACCCAAG660.10065885797950219No Hit
TTATAAAGGGATGCATTTCACTGCTTGCCACTGAACTTGTATTTGAATGACT660.10065885797950219No Hit
GACATCGGGGAATGCCACCACAATACATTTTAATCTCACGTTTCAAAATACA660.10065885797950219No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)