FastQCFastQC Report
Sat 31 Dec 2022
SRR5849483.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849483.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65018
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC5940.9135931588175583No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT3420.5260081823495032Illumina Paired End PCR Primer 2 (95% over 21bp)
GCAACTGGGAAGAATGTCAGTATACAAGAAGGAATAGAAAACTGATACTGTT870.13380909901873325No Hit
ATCATGGGGAGTGAGGCTGAAAGGGGGGGCTATGGAAGAGCGGTAGGGAGTC850.1307330277769233No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC840.12919499215601835No Hit
TTATTTGGGGAGGGGGCGCGGGGACACAGCCAGGCGCCCCTGCCCGCCGCGG810.12458088529330338No Hit
CTGCGAGGGATCATATGTAACATTTACTCCTGTTTCTGCTGATTGTTTTTTT780.11996677843058845No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC780.11996677843058845No Hit
TCAAGGAGGGTCTGCACTTGGGATGTCCCAGAAACACATCTTCCACCTCAGC780.11996677843058845No Hit
GTGTGTGGGCACCAGACTCGAGGACACCCTGGCCCTGCTGAGGCATACAGAG770.11842874280968346No Hit
GAAATTGGGCAGCAAACAAAGTACTTCTTCAGGGAAACCTGAAATTTCTAAT770.11842874280968346No Hit
ATACGCGGGAAGATTAAATCCGGCATACAGAATACAGCCATGCTTCTCAAAG750.1153526715678735No Hit
GAAAACGGGCGAGAGAAAACTCAGACAGGGAGGGCGCAGGGCCAGAACGGCT740.11381463594696854No Hit
CATCGCCGGGAGATGTATGCATATATTCCACATTTTGAAAAAGTCAAATACA740.11381463594696854No Hit
CAGAACGGGATTTGCCTACAAGGTACAGCCTCGGAACTGGCTTCTGTTTGCA730.11227660032606356No Hit
GAAGCTGGGGCCTCTGGAGCTTTTTCCCTTTATAGCACCATTGAATCCCAGT730.11227660032606356No Hit
GTTAGCGGGACATAAAGAAGCTCTAGTTCTTATAGAAATAACAAAGCAAATA720.11073856470515857No Hit
GTGGCCGGGAGAGATCTCAAACTCCGATGCCCCTGGGGCCACGTATGTTGTG720.11073856470515857No Hit
CAGTTGGGGAAAGCACTATAAGGCTGAATAGGCACAAGCGATTAAAAGTAGA720.11073856470515857No Hit
GGCGCAGGGAAGAATGGGGACGGCACCATTGACTTCCGAGAGTTCATCTGCG710.1092005290842536No Hit
ACAGTAGGGGGGCCCTGGGCTTCGAACCCGGCTGCTGATCACAGGCCTGTTC710.1092005290842536No Hit
CTGGTGGGGGGTAACCGGTAGCGCTTGCAGCATGGCTGACCAACTGACTGAA700.10766249346334861No Hit
ATTGTCGGGATGGCCAAGTGTTAAAATCTGTGTTTGGAAAATAGCGAATTAA700.10766249346334861No Hit
ACAACAGGGGCCGGCGTCGGGCGGGGCCGGCGGGGTCTTCAGGGTACCGGGC690.10612445784244362No Hit
TTTGCTAGGGAGTGTGGACTGGAGCCGAGATGGAGGCCTCATTTGTACCTCC690.10612445784244362No Hit
AACTCTGGATTCACGTGTAACTTTTAAGTTTTCAGTACAGTGCTTTTATACC680.10458642222153866No Hit
TTATAAAGGGATGCATTTCACTGCTTGCCACTGAACTTGTATTTGAATGACT670.10304838660063367No Hit
CGAAGCGGGATCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAA670.10304838660063367No Hit
TGGTATGGGAGTGCTTAATATGTGTAAGGACTTCCTCTTTAAATTTGGTACC670.10304838660063367No Hit
ATTTGCGGGGGTGGAGAGAGCGCGGAGACAAGCGCAGAGCGCAGCGCACGGC670.10304838660063367No Hit
TCATATGGGGCTGTAGTGGCTTCGTCTTCGGTTTTTCTCTTCCTTCGCTAAC660.10151035097972869No Hit
ATGCGCGGGGTTCCCAGGACGGGCGCGTCTTCAGGGTGGAAGCCTGGCGCAC660.10151035097972869No Hit
GGTAAGGGTTGCCATAAGAAACTGCTGAGTACTTGGCAAACCCTTTCATAGT660.10151035097972869No Hit
AAGCGAGGGAGGGAAGAGACAAATGACAATATTCAAATTGTTCTTGCAATAT660.10151035097972869No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)