FastQCFastQC Report
Sat 31 Dec 2022
SRR5849464.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849464.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120802
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC10800.8940249333620304No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT8210.6796245095279879Illumina Paired End PCR Primer 2 (95% over 21bp)
TTTCTGGGTACTGGCAGTATTGATTAAAAATATATATACACAGAAATTTTAA1960.16224896938792405No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1800.1490041555603384No Hit
CGACGGGTGACATTGTGGACATCGGATACCCAAGGAGACGAAGCTGAAGCAG1680.13907054518964918No Hit
AACGTTGGGAGACCCAGAAGTCAGACTCCAAATTTACTCGATCATTTTGCAT1640.13575934173275275No Hit
TTCTATGGGTGGTATTTCGAAGATTGTTGCACACTATAGAAATTTTTAAGGA1550.12830913395473584No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1510.12499793049783943No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1500.12417012963361533No Hit
CTCATGGGAGCCTTAATGAATTTAATTCTTTCACAAAAATGATTTCAAATTA1480.12251452790516713No Hit
CATAAAGGGAAATTCAGTATGATTAACAGGATGAAAGAACAACATGCTTAAA1480.12251452790516713No Hit
GGCACAGGGGTGTTGTGCCAATGGTGGAGAAGAAAACTTCGGTTCGCTCCCA1460.12085892617671892No Hit
TACAAGGGGATTACAGGGTAGACACGTTTTAACTGAAATCAATCAAGATAAC1430.11837552358404661No Hit
TGTCAAGGGAACCAAAGGGGTGACCAGCCCTCAGATGTGCTTATTGGATTCC1420.11754772271982251No Hit
TTTGGCGGGACTTTAGAATGCCCTTAGTTGAGAGGAGGGGTCCATTCAGACG1410.11671992185559842No Hit
AAACTTGGGATGTGAGGATAGTGTTGGTGTTTGGGGTTTTTCATCTGTACTG1370.11340871839870201No Hit
TGCCACGGGGCAGTGCATCCTGGGTTGGCGTAGCCATGGCGTCTCGTGTCCT1360.11258091753447791No Hit
TCTCCTGGGATGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGC1350.1117531166702538No Hit
TTTATAGGGATCAGTAGTGGAGCTGGTATGTTGTTTCATAGGATGGAAACAT1340.1109253158060297No Hit
TACAGTTATTACTTCCATTTAATTTATTTAAAAAATAAATTTATAAGCCCAC1340.1109253158060297No Hit
ACAGACGGGATCAAAACAAATGTTGATCTCTGTGAGTTGAATGCAAACATCC1340.1109253158060297No Hit
GATGCTGGGATTACAGGCATGAGCCACCATGCCCGGCCTGTGTTTTCTTTTA1320.1092697140775815No Hit
TTTCATGGGACTTCCAAAATGATATGATTTTCAGTCCTACTCTACCTCGAAC1320.1092697140775815No Hit
GTCGGGGAGAGTTAGTAAACTAATATATTTTGTACATTTTGTTTTACAAGTC1320.1092697140775815No Hit
ACAAGCGGGACTGAACAACACAGATACCATTGTGAAAACAGACACAGTCCTT1310.10844191321335739No Hit
TAACATGGGACAGATTGAACTCCTCGTTCTACTCATTGCTGTCTTCTCACTG1300.10761411234913329No Hit
CCTAGAGGGAGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCA1280.10595851062068509No Hit
CTTCGAGGGATAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGT1280.10595851062068509No Hit
CGCTTTGGGATTCCCTCTCTGAAGGGAAGTTAAATAAACAACATTTTTAAAA1270.10513070975646098No Hit
GACTAGGGGGTGATCAGGAAGACTAGGAGCTATTTCTGACTGTAAATGAATT1270.10513070975646098No Hit
AGAACTGGGATCAAGGGAGGGGCCTGCTCTATCCCCAGAAGGATCAGGATCA1260.10430290889223688No Hit
ATGGCCGGGATAGTCTCTATGTAGAATTACCTTGTTTGGTAATACTCACTTT1250.10347510802801278No Hit
TACCCTGGGACCTCTTCCCAGCCCTAGTACTCCCATGTGAAATCCTCCTGTA1240.10264730716378868No Hit
TCGACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1240.10264730716378868No Hit
TGGGATGGGAATATTTAGGAACCAAGATCTGAGAAATAGACATGTTTTTTGT1230.10181950629956457No Hit
GATACAGGGATCTAAGCCCAATCTAAAATTGTGCTTTTCTCACTTTTCTCAC1230.10181950629956457No Hit
TGCGCTGGGATATTATTTTGTTTTAGGTTTGCCATAAACTGGAACTTGCAGA1230.10181950629956457No Hit
GTAGGGATATTTATGGAGTACAACCTGATGTTTTGATATATGTATGCATTAT1220.10099170543534047No Hit
TGCGATTGGGCTGTATGTTAAGCTCTATTCTGCCAATCAGGAAACTGAGAGT1210.10016390457111637No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)