FastQCFastQC Report
Sat 31 Dec 2022
SRR5849463.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849463.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120291
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC10490.8720519407104438No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT8660.719920858584599Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1750.14548054301651828No Hit
TACAGTTATTACTTCCATTTAATTTATTTAAAAAATAAATTTATAAGCCCAC1680.13966132129585754No Hit
TTTCTGGGTACTGGCAGTATTGATTAAAAATATATATACACAGAAATTTTAA1650.1371673691298601No Hit
TTTCATGGGACTTCCAAAATGATATGATTTTCAGTCCTACTCTACCTCGAAC1620.13467341696386262No Hit
AACGTTGGGAGACCCAGAAGTCAGACTCCAAATTTACTCGATCATTTTGCAT1620.13467341696386262No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1560.12968551263186773No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1540.1280228778545361No Hit
TTTGGCGGGACTTTAGAATGCCCTTAGTTGAGAGGAGGGGTCCATTCAGACG1500.12469760829987281No Hit
TAACATGGGACAGATTGAACTCCTCGTTCTACTCATTGCTGTCTTCTCACTG1490.12386629091120699No Hit
AGTATGGGGTAGTATGAACAATTAAGAGGCTCTCCCCCTCTTGTCTTTAAGC1450.12054102135654371No Hit
ACAGACGGGATCAAAACAAATGTTGATCTCTGTGAGTTGAATGCAAACATCC1450.12054102135654371No Hit
TTGTGCGGGATTGATTTGAGATATAGGCATTTAAAGCTGTACATTTTTTCCT1440.1197097039678779No Hit
TGTCAAGGGAACCAAAGGGGTGACCAGCCCTCAGATGTGCTTATTGGATTCC1440.1197097039678779No Hit
ACAAGCGGGACTGAACAACACAGATACCATTGTGAAAACAGACACAGTCCTT1430.11887838657921208No Hit
CGCTTTGGGATTCCCTCTCTGAAGGGAAGTTAAATAAACAACATTTTTAAAA1430.11887838657921208No Hit
TTAAAAGGGGATCGCGGGATGAACTAGTTGTTTTGTTTTTTAAATGGACAAA1430.11887838657921208No Hit
GATGCTGGGATTACAGGCATGAGCCACCATGCCCGGCCTGTGTTTTCTTTTA1410.11721575180188043No Hit
CTCATGGGAGCCTTAATGAATTTAATTCTTTCACAAAAATGATTTCAAATTA1390.1155531170245488No Hit
TTTTGGGGGATTGCAGCTTTGCTTTCTTCTTCCTGTTGGAAATATATTTTCA1390.1155531170245488No Hit
TTCTATGGGTGGTATTTCGAAGATTGTTGCACACTATAGAAATTTTTAAGGA1380.114721799635883No Hit
TACAAGGGGATTACAGGGTAGACACGTTTTAACTGAAATCAATCAAGATAAC1350.11222784746988552No Hit
TTAACAGGGCTGTAAGCATCATCTCTTTCTATTTTCACAGTTTATTAAACTG1350.11222784746988552No Hit
AGAACTGGGATCAAGGGAGGGGCCTGCTCTATCCCCAGAAGGATCAGGATCA1340.11139653008121972No Hit
GATACAGGGATCTAAGCCCAATCTAAAATTGTGCTTTTCTCACTTTTCTCAC1330.11056521269255389No Hit
TGCCACGGGGCAGTGCATCCTGGGTTGGCGTAGCCATGGCGTCTCGTGTCCT1330.11056521269255389No Hit
CAAAATGGGACATGCTGTGCTCTTTGAAGAGATTTCAACAAGAATATTGTAT1320.10973389530388807No Hit
CGACGGGTGACATTGTGGACATCGGATACCCAAGGAGACGAAGCTGAAGCAG1300.10807126052655644No Hit
GACTAGGGGGTGATCAGGAAGACTAGGAGCTATTTCTGACTGTAAATGAATT1300.10807126052655644No Hit
GTAGGGATATTTATGGAGTACAACCTGATGTTTTGATATATGTATGCATTAT1290.10723994313789062No Hit
AAACTTGGGATGTGAGGATAGTGTTGGTGTTTGGGGTTTTTCATCTGTACTG1280.10640862574922479No Hit
TGCGCTGGGATATTATTTTGTTTTAGGTTTGCCATAAACTGGAACTTGCAGA1280.10640862574922479No Hit
CTAATTGGGTTGCTCCGAATGTCAAAAACAAATGCGACAAACAATGGCACTT1270.10557730836055898No Hit
TGCGATTGGGCTGTATGTTAAGCTCTATTCTGCCAATCAGGAAACTGAGAGT1270.10557730836055898No Hit
ACATTAGGGATATTGCCTTCTTGCCTTATCTCTGATTCCATTGCTGTCTATC1270.10557730836055898No Hit
ATGCAAGGGACCACTTGTCCCATCAAATCCTGAAAAAATGAGAGCAGATGTT1270.10557730836055898No Hit
TCTCCTGGGATGGCTGGACAGCAAACATGGAGAGAATCATGAAAGCTCAAGC1260.10474599097189316No Hit
TGGCGGGATGATTCATATTGCATTACCTTGAAATATGAAGTGCCATTTGAAT1250.10391467358322735No Hit
TTATCAGGGAACATGGGAGAGACAGCACAGTTTGATAATTATTTGTCACAGG1250.10391467358322735No Hit
GTCGGGGAGAGTTAGTAAACTAATATATTTTGTACATTTTGTTTTACAAGTC1230.1022520388058957No Hit
TTACACGGGACTGCAGAGGCAATGGCAAAAATATTTTCAGTTGCTCTTGGAG1230.1022520388058957No Hit
GTTGTTGGGAAACTTGGAAGAGGGTCAAGTGGGCAACCTGAGAGATTCAAGT1220.10142072141722988No Hit
GAGTGTGGGCTAGTAGCGGGAATAGGAATTGCATTAGTCCAAGGCCTTTTTC1220.10142072141722988No Hit
GGCACAGGGGTGTTGTGCCAATGGTGGAGAAGAAAACTTCGGTTCGCTCCCA1210.10058940402856408No Hit
TCTATAGGGAGAAATACAAATTTTTTAAATACTTTTTTTTATTCTCGGTTTT1210.10058940402856408No Hit
CCTAGAGGGAGAGAATAAATTAAAATCAGCAGATTGAGTTTTCAACATTGCA1210.10058940402856408No Hit
GCTCTTGGGATAAGTGCACATTTACATGTACACGCATGTACAAAACCAAGAC1210.10058940402856408No Hit
TATGTTGGGAACCCAAGTTTTCTAATGGACAAAGAACTTGAATAGACATTTC1210.10058940402856408No Hit
GACAACAGGGACATGGGAACTCTGTAATGTTTCTTCAGTTTTGCTATAAACC1210.10058940402856408No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)