FastQCFastQC Report
Sat 31 Dec 2022
SRR5849462.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849462.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120156
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC10100.8405739205699256No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT7850.6533173541063284Illumina Paired End PCR Primer 2 (95% over 21bp)
TTCTATGGGTGGTATTTCGAAGATTGTTGCACACTATAGAAATTTTTAAGGA1770.14730849895136322No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1730.14397949332534374No Hit
CTCATGGGAGCCTTAATGAATTTAATTCTTTCACAAAAATGATTTCAAATTA1600.13316022504078032No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1600.13316022504078032No Hit
CATAAAGGGAAATTCAGTATGATTAACAGGATGAAAGAACAACATGCTTAAA1560.1298312194147608No Hit
CGACGGGTGACATTGTGGACATCGGATACCCAAGGAGACGAAGCTGAAGCAG1500.12483771097573154No Hit
TGTCAAGGGAACCAAAGGGGTGACCAGCCCTCAGATGTGCTTATTGGATTCC1480.12317320816272179No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1460.12150870534971205No Hit
TTTCTGGGTACTGGCAGTATTGATTAAAAATATATATACACAGAAATTTTAA1440.11984420253670228No Hit
TTTGGCGGGACTTTAGAATGCCCTTAGTTGAGAGGAGGGGTCCATTCAGACG1440.11984420253670228No Hit
ACAGACGGGATCAAAACAAATGTTGATCTCTGTGAGTTGAATGCAAACATCC1400.1165151969106828No Hit
CTTCGAGGGATAGACATAAAGGATAAAAGGAAAATAACCGTCTGCCGATGGT1390.1156829455041779No Hit
TACAGTTATTACTTCCATTTAATTTATTTAAAAAATAAATTTATAAGCCCAC1380.11485069409767303No Hit
AACGTTGGGAGACCCAGAAGTCAGACTCCAAATTTACTCGATCATTTTGCAT1380.11485069409767303No Hit
TGCGCTGGGATATTATTTTGTTTTAGGTTTGCCATAAACTGGAACTTGCAGA1370.11401844269116813No Hit
GGCACAGGGGTGTTGTGCCAATGGTGGAGAAGAAAACTTCGGTTCGCTCCCA1340.11152168847165352No Hit
GACTAGGGGGTGATCAGGAAGACTAGGAGCTATTTCTGACTGTAAATGAATT1340.11152168847165352No Hit
ATGCAAGGGACCACTTGTCCCATCAAATCCTGAAAAAATGAGAGCAGATGTT1340.11152168847165352No Hit
TTTCATGGGACTTCCAAAATGATATGATTTTCAGTCCTACTCTACCTCGAAC1320.10985718565864376No Hit
TGGCGGGATGATTCATATTGCATTACCTTGAAATATGAAGTGCCATTTGAAT1320.10985718565864376No Hit
GTTGCAGGGATAATTTCAAATATGCTGACAGAGAAAACTAGTAACCAAGTTC1320.10985718565864376No Hit
TCAACTGGGATGGTCATTGTAGAAAATTTAGGAGAAAATGCAGAAAATTATA1310.10902493425213888No Hit
GATGCTGGGATTACAGGCATGAGCCACCATGCCCGGCCTGTGTTTTCTTTTA1310.10902493425213888No Hit
TATGTTGGGAACCCAAGTTTTCTAATGGACAAAGAACTTGAATAGACATTTC1310.10902493425213888No Hit
TGCGATTGGGCTGTATGTTAAGCTCTATTCTGCCAATCAGGAAACTGAGAGT1300.10819268284563402No Hit
GATACAGGGATCTAAGCCCAATCTAAAATTGTGCTTTTCTCACTTTTCTCAC1290.10736043143912913No Hit
GATGGGGACCTGGAAACCAGAATGATTTTGACATACAGGAGAGCTGCAGTTG1290.10736043143912913No Hit
GTCGGGGAGAGTTAGTAAACTAATATATTTTGTACATTTTGTTTTACAAGTC1290.10736043143912913No Hit
TTGTGCGGGATTGATTTGAGATATAGGCATTTAAAGCTGTACATTTTTTCCT1280.10652818003262425No Hit
AGAACTGGGATCAAGGGAGGGGCCTGCTCTATCCCCAGAAGGATCAGGATCA1280.10652818003262425No Hit
ACAAGCGGGACTGAACAACACAGATACCATTGTGAAAACAGACACAGTCCTT1270.10569592862611937No Hit
ACTCAGGGATGTTCGTAAATACCAAAGTCGGCGGGAAAGTGAAGTTGTCTCC1270.10569592862611937No Hit
GCATTTGGGCTTAAAAAACAAGACCAAAATATGGGACCATAATTTATGCAGC1260.10486367721961451No Hit
AACGAAGGGAGTGATAAGAAGAAGGATATGTCTCCACCCTTTATTTGTGAGG1250.10403142581310962No Hit
CGACAGGGACTCTAAGTAAATTTTCTAATTTCACTAAGTACGTTTTTAGGCT1240.10319917440660474No Hit
GCGTGAGGGATATTGTGGAATTTCTTAGTTTTGCCTCTCCTTCAGTTTATTG1240.10319917440660474No Hit
CGCTTTGGGATTCCCTCTCTGAAGGGAAGTTAAATAAACAACATTTTTAAAA1230.10236692300009988No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1230.10236692300009988No Hit
GTTTATGGGAGATTTGGGATGGACATTTGCATGGGATTAAGAGATTAAGAAA1220.101534671593595No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)