FastQCFastQC Report
Sat 31 Dec 2022
SRR5849461.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849461.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139764
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC18861.3494175896511262No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT16221.1605277467731319Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC2500.17887295727082797No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2470.17672648178357803No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC2200.15740820239832862No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1920.13737443118399587No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1680.1202026272859964No Hit
CCTAAAGGGAGAGAACACTTTGTTTAACTAATGAATCTATCGTTATTTCTCC1630.11662516814057984No Hit
ACTTGTGGGAAAAAGAACAGCAGCTATTACATGATAAACAGATGGAAGAAGA1550.11090123350791334No Hit
TCGGCTGGGATCTCTGTTTGTAGAGAGCTAACGTTTGATAGTTCTAATTTAA1510.1080392661915801No Hit
CTTGTCTAGGGATCCCAGTCAATATGTGAGTTCACACTGGAGGTGGGAAGCA1460.10446180704616354No Hit
ATTTCCGGGAGCGAGGGACTCAACACGCTGATTCTCCTCCTGCCTCTCCCGT1410.10088434790074696No Hit
TCGAGTGGGCTAGCTTACAGTCTCCAGGTGTTGAATAATTTTCCTCAGTGTA1410.10088434790074696No Hit
AAGTTATGGGACAAAAATGAAGTGGCATAATGACTGCATAGTTTATTTTATT1400.10016885607166366No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)