FastQCFastQC Report
Sat 31 Dec 2022
SRR5849460.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849460.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138480
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC18011.3005488157134604No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT16461.1886192952050838Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC2330.16825534373194684No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC2070.1494800693240901No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2050.1480358174465627No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1900.1372039283651069No Hit
CCTAAAGGGAGAGAACACTTTGTTTAACTAATGAATCTATCGTTATTTCTCC1640.11842865395725014No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1630.11770652801848641No Hit
ACTTGTGGGAAAAAGAACAGCAGCTATTACATGATAAACAGATGGAAGAAGA1620.1169844020797227No Hit
TTATCCGGGACACGACGCTTTTCCGATCTTGTGAAGGGGTTATACAGAATTG1540.11120739456961294No Hit
GCTAACGGGAGGAAAAAATATATCTACTCCATCTTCCCAGAAGAAGTTACTA1540.11120739456961294No Hit
TTCGCGGGGCAGCTGGCTCAAGTCAATGTGCCTGTCTCAAAATGATAAAGAT1520.1097631426920855No Hit
GTTACAGGGAAATGGAAACCATTTCTACTTTAAGGAAAACTAATGCGTTACA1510.10904101675332177No Hit
AGCTTAGGGACAACTAATCCAGTGGTTAAATTTTTAAAACCCAAAAGTAAAG1500.10831889081455806No Hit
GCTTATGGGGCATCAGATTTTAGAACTAAATTTAAATTCACTTTTAACATTC1460.10543038705950318No Hit
TCGGCTGGGATCTCTGTTTGTAGAGAGCTAACGTTTGATAGTTCTAATTTAA1440.10398613518197573No Hit
AAGTTATGGGACAAAAATGAAGTGGCATAATGACTGCATAGTTTATTTTATT1390.10037550548815713No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)