FastQCFastQC Report
Sat 31 Dec 2022
SRR5849459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136834
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC16571.2109563412602131No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT15571.1378750895245335Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC2440.17831825423505854No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC2120.15493225367964103No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2100.15347062864492744No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT2010.14689331598871624No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1770.1293538155721531No Hit
CCTAAAGGGAGAGAACACTTTGTTTAACTAATGAATCTATCGTTATTTCTCC1640.11985325284651475No Hit
AAGTTATGGGACAAAAATGAAGTGGCATAATGACTGCATAGTTTATTTTATT1600.11693000277708757No Hit
TCGGCTGGGATCTCTGTTTGTAGAGAGCTAACGTTTGATAGTTCTAATTTAA1590.11619919025973077No Hit
GTTACAGGGAAATGGAAACCATTTCTACTTTAAGGAAAACTAATGCGTTACA1570.11473756522501719No Hit
TTATCCGGGACACGACGCTTTTCCGATCTTGTGAAGGGGTTATACAGAATTG1490.10889106508616281No Hit
ACTTGTGGGAAAAAGAACAGCAGCTATTACATGATAAACAGATGGAAGAAGA1420.10377537746466521No Hit
TCTTGTGGGACTGCTCATCCCTGATAGCACCCAGTGATTACTGCAGTATCAA1400.10231375242995162No Hit
TATAGTGGGCTTCCGATCTGGCAAAGGGGGGAAGAGACGAGTGCGGTAACAC1400.10231375242995162No Hit
TCATGTGGGGGGGCCCTGCCCACCACAAGTGAGTGAGCCCCACAGTCTGCCC1370.10012131487788123No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)