FastQCFastQC Report
Sat 31 Dec 2022
SRR5849446.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849446.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107806
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC12901.196593881602137No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT12851.1919559208207335Illumina Paired End PCR Primer 2 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1860.17253214106821513No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1800.16696658813053078No Hit
AAAGGTGGGAACCAAAAATGTGAAAGATCTCTACAAGAAAAACTACAAAACA1660.1539802979426006No Hit
GGTTGTGGGGATATTGTCATAAGGACCGTGGGGGATTCAGATCCTTGACCGA1660.1539802979426006No Hit
TATAATGGGGAATGCAGAGGAAAAGTTCTTAAAGGAAATTAAAAGTGTTGCT1640.15212511363003914No Hit
TTTCGCGGGAACATAGAGATAGGAAGTCATCTGACTCTGACAGCTCAGACTC1580.14655956069235476No Hit
GGCATAGGGGCTTTGTTGAAGTTATTGTGGAATGCAAAGTAGCACTGCAAAC1560.14470437637979333No Hit
CCATGGGAGTCTTCCGGTCTGCAGCACGGAAAAGCACATAATCTTTCTTTGC1550.1437767842235126No Hit
TACCTAGGGGCCTGAAGAGGAGTACTCTCTACTTGTTGATACAGAAGGTGTT1550.1437767842235126No Hit
GACCATGGGAAAGAGATTACACTATAATTATGTTTAAAACCTTACTTGCACT1510.1400664155983897No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1500.13913882344210896No Hit
TTCCTAGGGGAGAAATTAATGGCTAATTAATTTGCCCAAGGACAAAGAAAGT1490.13821123128582824No Hit
GCCTGCGGGGTGGCAAAGATGTTCCCTTGTAGGCCCCTTTCAGGTAACCGTC1470.13635604697326678No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1460.13542845481698607No Hit
GCGTTGGGGTGTCCTCAATTCTGCAGCTTTTTTCATACTTTTGAATGTTTCT1440.1335732705044246No Hit
ACGCGGGGTGGATGTTTGAATTATCTATGTATTTGGCCAAGGTTCATTATAT1430.1326456783481439No Hit
ACTTCAGGGCACCTCAGTAAAAACTGAAAAAATTTAAGGTGCATGCCAATTA1430.1326456783481439No Hit
AAAGCAGGGACGTGCTCCAAACGCTGAGTAGAGATTCTTGGATTGTGATGAA1430.1326456783481439No Hit
GATTCTGGGAACTCCTGCAATTCACGGCATCCAAGATTCCTTACAGCCTCCG1410.13079049403558243No Hit
TTAGCCGGGCTGGCGTTGGGGTTGCAGGTGCAAAGTGACTTTGTTTATTCTT1410.13079049403558243No Hit
CGCCGGGTAACAAACTGAGAGTCAAATTTTCTAAGTTTTTAAATATGCATTT1410.13079049403558243No Hit
TAAATAGGGAATTTAGTATTCGGTAGTTAACATTCCTATTCACTATAATAAA1400.1298629018793017No Hit
TCACCTGGGATATTCTAATTGTTAGCAACTCATTCAATTGGGATTTTTCTTG1390.12893530972302097No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1380.12800771756674026No Hit
CGCGCGGGGGGGTTTGAGGGAAGAGAGCTTTTCGTAGGAAGGGTGAAATCCC1370.12708012541045952No Hit
CACAAGGGGATATACAACTTGCAGTCATTGTAAATATGCGTGAGTTAGGGGT1370.12708012541045952No Hit
TCTGCTGGGGAGTTGCAGACAGCCCGGCGAACCGCGCAATGCGCTTCTTCTG1350.12522494109789808No Hit
AGACGGGAGATGGTCAGAATGCAGATAAATTGTGTGCATCTCAATCAGTAAA1340.12429734894161734No Hit
ACTGCTGGGTGGTAGAACTAGTGATAACAAAAACAGCAACGATTACTGCTAT1340.12429734894161734No Hit
AATAAAGGGTATTCTCTCACCAGTAGGACTTAAACTTTGTTTCTCCAGTGGC1330.12336975678533663No Hit
TATCATGGGGAATGGATGATGTCACGACTTAAGATTAGATGATCTCTACTGA1330.12336975678533663No Hit
CGCTTAGGGCTTACTAATGATCCCAGCACAGTGCTTTCTGTATATTATGTCT1330.12336975678533663No Hit
CGCAACGGGCTAGGTAGGGGAACTTCTTTAGTGTTGCACTGGCAATAATGTA1320.12244216462905588No Hit
TCATCAGGGGATCACATTTGCATTTTAGCTAGTACACTGTACCAGCAGTTAG1310.12151457247277517No Hit
AGTTTCGGGAAGGGGAAAATCACATCCTCTTTGGAGATGATTATATTTTGAT1310.12151457247277517No Hit
ATTCTGGGGCAACAAAGAAAGACTAGAGATTTACTTAGTAACAAATGATGTG1310.12151457247277517No Hit
CAACTGGGAAGAGTATTAGGTTGCCGTCATCAGCCTTTGTAAATAAGTTAAA1300.12058698031649445No Hit
AGCTGGGATCCTGAAAAATGTTAAGTAAGGAAAAAGCATGCCTCCCTGTGCT1300.12058698031649445No Hit
TGCATAGGGAATGAATGAATACAGAGTTCCTGAACTGAATGTTCAAAATGGA1290.11965938816021371No Hit
AATTCAGGGAACTCAAGATGGATTAAGGACTTAAATGTAAAACCTAAAATTA1280.118731796003933No Hit
TGCCATGGGATTCCTTTAATACATATTTTTTGAGTACCTATTTTGTGCCAAA1270.11780420384765225No Hit
TTTGACGGGTGAGTAAAGATTCTTAACTTTTTAAAGCTGTCGTTAGAAGTTT1260.11687661169137153No Hit
TAGAGGGAAGTGTTAAAAAAAAGTCTACATAAGAGTCTAGAAGTTTGTTGCC1260.11687661169137153No Hit
CAGTTAGGGGGCCATTGGCAATTGCGCAGTTTTAGGCTTTTCTGTTCTTCCC1250.11594901953509082No Hit
CAATACGGGTCAGTCTTCTCTGTGACGGCTAGTTTAGACATATCTGTGGACC1240.11502142737881008No Hit
GAGCCAGGGATTACAAATAAAGAATTGAGCCGCATACAAAGCAATTAGCTCT1240.11502142737881008No Hit
GCACAAGGGCAAAAAGTTAAATGTAGACACAGAAGATATTTTGAAAGCCCAC1240.11502142737881008No Hit
GCATCAGGGCGATCTCTCAGCGGGAGCTTCAGTGACCCAGGGTCTGAACTGC1240.11502142737881008No Hit
TGCTGCGGGGTGGCCCAGGGGTTGGGGACCCCTGCCTTACACCACATGGCGT1230.11409383522252936No Hit
TATCATGGGGTTATGTTTTTGTGCTTGTTCCCCACTTGATTCATCTGCAGAA1230.11409383522252936No Hit
TTTGCCGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC1230.11409383522252936No Hit
ACCGCCGGGGCATACTGAAAGGTCCGAAAAGGGCATCCACGGCAGCTTGAGC1230.11409383522252936No Hit
GTCAGGGGGAAGGCAATAAAGAAAGACTTTCTCTTTGACGCTTGTGAATAAT1230.11409383522252936No Hit
CATCATGGGACTTAATCTATAGGTTTATACATGTATGAGAAATGGGAAAAAA1220.11316624306624862No Hit
TTCAGTGGGCTCCCAATTCCTTTGGATAACTTCATTGCCTAGTAGATTACAG1220.11316624306624862No Hit
AACTATGGGATCTGAAATTAGGAAGTTCAGAAATGTTAAAAATGTTAATTTT1220.11316624306624862No Hit
GAGCAAGGGTTAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA1210.1122386509099679No Hit
TTTAAAGGGCGATCTTTTACGGGGGCCCTCGAGGTGAAGGATTGGCCCTGAT1210.1122386509099679No Hit
TTATGCGGGAGGGCGCAGCTGCGCCCGCTTCAGCGCCTCCCGGGTCTGCGTG1210.1122386509099679No Hit
TGCCACGGATATTAGTGGTGGGTTAGGAAGTGGATTTAAAAGAAAAGAAATG1210.1122386509099679No Hit
GTGCTGGGCGCTGGCTCCTGTTTCCAATTGATTGAGGGATGCCCCCAAGGAA1210.1122386509099679No Hit
ATTGAGGGATTGAAAATACAGCTTTTACGTGGATGCAGGACTGCTATAAGAA1200.11131105875368719No Hit
GTTATAGGGCTACATAAAGAAAGGCAAATGAATGGCTAATATTCATAAAGAA1200.11131105875368719No Hit
GCAAATGGGGCTTTCCGAGCCCGCTTGCACCTCGGCGATCCCCGACTCCCTT1200.11131105875368719No Hit
CTTATAGGGACTCTTTCTGGTAGAGGATTTCCAGTTTTCATCAGATTTCCAA1200.11131105875368719No Hit
CCTGCGGGACTGAGGCATGTACCAAAAACATAGAATTAAGTTTTAGCCTCTT1200.11131105875368719No Hit
ATCTTTGGGACATAATAATGATCCATCCTGCTTTGCTGAAAGCATTTCTCTA1200.11131105875368719No Hit
TATCAGGGGGTACAAAGACCAATGACACTATGAAGAAACTGCATCAACTAGT1200.11131105875368719No Hit
ACAACCGGGCAACAAAAGTTACATATGCTACTAAGCTCAACAAAAAAGGAAC1200.11131105875368719No Hit
ACATAGGGGCTGTATTTATTATATTCTGTATGCTAGAATTATTTATTGCATG1200.11131105875368719No Hit
ATAGTGGGGAAGTAATCCTCCCACCATGGCCTCCCAAAGTGCTGGGATTACA1190.11038346659740644No Hit
AATTATGGGATACTTTAAGCTTTGCCTATTGATTTTCTACTGGAGGATGGTA1190.11038346659740644No Hit
TTGTTTGGGTGTCTAATTTAATTTAAAGATCAATAGACAAATGAAACGTAAA1190.11038346659740644No Hit
ATCATTGGGGGGGCTGACAGTGGGATTTCCTTTCCTGTTCTTTATGGGATGG1190.11038346659740644No Hit
ATTTATGGGAAGTGAGGCTGCACTTGATTATATTTGATTCTTTGTTCGTAAT1180.10945587444112573No Hit
ATGGTTGGGACAGCAATTCGAGAAATTAAAATTCTCCGGCAGCTTACCCATC1180.10945587444112573No Hit
GCTAAAGGGTCTAAAATATGTAGAGCAGGTATATAAATTCCACTTTCCTTTG1180.10945587444112573No Hit
TTCTCGGGGATGCCTACCTGGAAAATTCACCATTCTAAGACAAGGGACGTAG1180.10945587444112573No Hit
GTAATTGGGTAAAGGATTTGGAGATTAGGAGTGGATGTAGGGCAGAAGTCGG1170.10852828228484501No Hit
CGCTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1160.10760069012856427No Hit
AATAACGGGATCTGTTTTTGCATCTGTATAATGAAAGTATAATAACCTGTCT1160.10760069012856427No Hit
AGTTCCGGGAGCTCCTCTGTCTGTACCACGTCGGGGGTTGGGACCAATCATT1150.10667309797228355No Hit
TGACCTGGGATAGATGCAAGAAAAAAATGTTTATGAATTGCATTTGTATATT1150.10667309797228355No Hit
GCTTCGGGGAAGTATGTAAAGGAAACATATTTTTAAAGAAGCTTAACAGTAA1150.10667309797228355No Hit
CCTATTGGGAGGATAAATTAGTCATAGATAATAATATAAAATTCATCAGTGG1150.10667309797228355No Hit
TCTTGCGGGATTCCTTCAAAACACTTCCATTTATAACTTGAAGATATAAAAA1150.10667309797228355No Hit
TCACACGGGTAGCGTGCACACATCTAGTTTATTCACAAAGGTATAATTTCCT1140.10574550581600281No Hit
ATCAAGGGGAATATTCTAAGATACAATGTTTTGAATAATGTTTATTTCTCAT1140.10574550581600281No Hit
ACAGTGGGGTTCGTAGGCTGCAAGGCTATATGGTACAGCCTGTTGTTCGTAG1140.10574550581600281No Hit
TCGCGGGGATCTAGTCGCGGGAGCACTAAATGCCCACTGGAGAAAGCGGGAA1130.1048179136597221No Hit
ACACATGGGAACCAACACCATTACCTAATTCCAGAACATTTTTATTGCCCTC1130.1048179136597221No Hit
TTATAGGGGAACATGTTAATCATAGTTTTAAAAAGTTCCCTGTCTGATAATT1120.10389032150344138No Hit
GGGACGGGGAAGAGACATACATGACAAACCCACAGCAAACATACTGAATGGG1120.10389032150344138No Hit
TTCTGCGGGACCTCCTCCTGCAGCAGCGGCAGCGGCAGCAGCAGTTAGCAGC1120.10389032150344138No Hit
TTTACTCGGGATTGTAAACACAGCTGCATGGGGAGGTCAGCTTGAGGTAAGT1120.10389032150344138No Hit
TACCTAGGGCTTCTGCTGAGTACCACCGCAAAGCCCTGTGAGCGTCTACAGA1110.10296272934716064No Hit
CTCTAAGGGAAGGTTTTTATGAGTAACCAAGGAACTGGTGTTCGAGAGTGGC1110.10296272934716064No Hit
TATACCGGGGAAAGTTTGATTGTGTTAATATATGTAAAATACTTTAAAATAG1110.10296272934716064No Hit
ACTCGCGGGGACGCTCTTCCGATCTATTCTAGGGGAAGTTGCAGTTGTTGAC1110.10296272934716064No Hit
CCTCGCGGGCTGTCTAGATCTGTGAGAACATTAGTCTTTTATAATACTTTAA1110.10296272934716064No Hit
TTTACCGGGAACACCAAGAAAGCTGAAAAGACATTATTTGGACCCATCAAAT1110.10296272934716064No Hit
GCATGTGGGGGATAGATTTACATTTCTAGGCATACTCTCAGGTCCTCTATGT1100.10203513719087992No Hit
ACTACAGGGTGCAACACAATGTTTCCAACTCTCTAACATGTTTAACCCTCAA1100.10203513719087992No Hit
GGTCAAGGGGCTTTCTGCTCCGTGACGCACGGAGGCTTGGCAGCCACTCTTA1100.10203513719087992No Hit
GCGTTTGGGGAAATGTGGGCTTATCTGGACATAACCCATCATTAAGTTTAGG1100.10203513719087992No Hit
ATAATTGGGACTCAAGAGATTCTCCCCTAAATATTGATCTTTCTAAGTTTAC1100.10203513719087992No Hit
GCCATGGGGGGCTACCGTGTCAACGAGGCTGCTTTACAACAGCTTTATTTAT1090.10110754503459918No Hit
TTTTCTGGGCTGCGAGTGGGGTGTACAGTCTGCATTAGCTTAGAATGGAATT1090.10110754503459918No Hit
AACAGTGGGGAAGTAAAAAAACAATAATGCATATTTCCTCATATCATAAAAA1090.10110754503459918No Hit
AGTGCTGGGATTTGTGCAGGAAACCAGCCATTTAATTGTTCTAGAGTTTTAG1090.10110754503459918No Hit
AGAGGGGATCCTCACCTTCATCCACAAAGGTAAACACTAGCCTGACTTCTAT1090.10110754503459918No Hit
TAAATTGGGCTCAGAAAGACTGCATTTCCCAGCCTTTACCACTTCTCAACCT1090.10110754503459918No Hit
TTGCTTCGGGAACTCATGTAGGTTTTTATTGTGTGCTAACAAAATTAGGCCT1090.10110754503459918No Hit
ACAATGGGGTACAAAAGAATACCTAGTGAGGCCAGGCGCAGTGGCTCACATC1090.10110754503459918No Hit
TGCTTGGGGACCAGCTGTGTCCCTTGACAACAGGTCATGGTTCCTCTGAGGG1090.10110754503459918No Hit
AGAGAAGGGATATATTTTAATTATATTTGACAGGTTTTGCATCTAAATTGAC1090.10110754503459918No Hit
AAATACGGGAGAAACTGTCATTACCTGCAGCAATTTCCAACTCATTCCTACA1080.10017995287831846No Hit
TCCTAGGGGGAGTACCTGTTTTGTAAACAGCTTTATTGAGACATAATTTACA1080.10017995287831846No Hit
GTAGATGGGATGTACACATACACATAAATATTATTGCAATTAAGTGATCAAG1080.10017995287831846No Hit
AAGTTAGGGGTGGTGGGAGAATGGGCATGCACATGCACACACATGAAAAATA1080.10017995287831846No Hit
TAGCTGGGGACTAATTAAAACAACAACAACAAATAGAATTCAGGATATAAGG1080.10017995287831846No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)