FastQCFastQC Report
Sat 31 Dec 2022
SRR5849445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107326
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC12991.210331140636938No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT12561.1702662914857536Illumina Paired End PCR Primer 2 (95% over 21bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1950.18168943219723088No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1830.17050854406201665No Hit
GGCATAGGGGCTTTGTTGAAGTTATTGTGGAATGCAAAGTAGCACTGCAAAC1770.16491809999440954No Hit
TTTCGCGGGAACATAGAGATAGGAAGTCATCTGACTCTGACAGCTCAGACTC1730.1611911372826715No Hit
CGCCGGGTAACAAACTGAGAGTCAAATTTTCTAAGTTTTTAAATATGCATTT1730.1611911372826715No Hit
GGTTGTGGGGATATTGTCATAAGGACCGTGGGGGATTCAGATCCTTGACCGA1700.15839591524886792No Hit
GACCATGGGAAAGAGATTACACTATAATTATGTTTAAAACCTTACTTGCACT1630.1518737305033263No Hit
TATAATGGGGAATGCAGAGGAAAAGTTCTTAAAGGAAATTAAAAGTGTTGCT1600.14907850846952278No Hit
GATTCTGGGAACTCCTGCAATTCACGGCATCCAAGATTCCTTACAGCCTCCG1590.14814676779158825No Hit
TACCTAGGGGCCTGAAGAGGAGTACTCTCTACTTGTTGATACAGAAGGTGTT1590.14814676779158825No Hit
CCATGGGAGTCTTCCGGTCTGCAGCACGGAAAAGCACATAATCTTTCTTTGC1580.14721502711365372No Hit
GCCTGCGGGGTGGCAAAGATGTTCCCTTGTAGGCCCCTTTCAGGTAACCGTC1550.14441980507985017No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1520.14162458304604664No Hit
TTGGACGGGATATTTAAAAGTAATCTGTAGATTACTTACAATACCTAATACA1510.1406928423681121No Hit
CGCGCGGGGGGGTTTGAGGGAAGAGAGCTTTTCGTAGGAAGGGTGAAATCCC1500.1397611016901776No Hit
AGAGAAGGGATATATTTTAATTATATTTGACAGGTTTTGCATCTAAATTGAC1490.13882936101224308No Hit
TTTGCCGGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAAC1480.13789762033430855No Hit
GCACAAGGGCAAAAAGTTAAATGTAGACACAGAAGATATTTTGAAAGCCCAC1450.135102398300505No Hit
AAAGGTGGGAACCAAAAATGTGAAAGATCTCTACAAGAAAAACTACAAAACA1430.13323891694463597No Hit
GAGCCAGGGATTACAAATAAAGAATTGAGCCGCATACAAAGCAATTAGCTCT1430.13323891694463597No Hit
ACTGCTGGGTGGTAGAACTAGTGATAACAAAAACAGCAACGATTACTGCTAT1400.13044369491083244No Hit
GCGTTGGGGTGTCCTCAATTCTGCAGCTTTTTTCATACTTTTGAATGTTTCT1390.1295119542328979No Hit
CAGTTAGGGGGCCATTGGCAATTGCGCAGTTTTAGGCTTTTCTGTTCTTCCC1390.1295119542328979No Hit
TTCTCGGGGATGCCTACCTGGAAAATTCACCATTCTAAGACAAGGGACGTAG1380.12858021355496338No Hit
AAAGCAGGGACGTGCTCCAAACGCTGAGTAGAGATTCTTGGATTGTGATGAA1380.12858021355496338No Hit
ATTGAGGGATTGAAAATACAGCTTTTACGTGGATGCAGGACTGCTATAAGAA1370.12764847287702888No Hit
CGCTTAGGGCTTACTAATGATCCCAGCACAGTGCTTTCTGTATATTATGTCT1370.12764847287702888No Hit
TTTGACGGGTGAGTAAAGATTCTTAACTTTTTAAAGCTGTCGTTAGAAGTTT1360.12671673219909435No Hit
ACTTCAGGGCACCTCAGTAAAAACTGAAAAAATTTAAGGTGCATGCCAATTA1350.12578499152115982No Hit
GTTCAGGGGATCTAGCGAAGGGATCCTTGATATTCTGACACACAAAATTTAC1350.12578499152115982No Hit
TTCCTAGGGGAGAAATTAATGGCTAATTAATTTGCCCAAGGACAAAGAAAGT1340.12485325084322532No Hit
CACAAGGGGATATACAACTTGCAGTCATTGTAAATATGCGTGAGTTAGGGGT1340.12485325084322532No Hit
TACAGGGGAATCTTTTTGATAGCCATTGCACAATGCAGACATCTGTCACATG1330.1239215101652908No Hit
AGCTGGGATCCTGAAAAATGTTAAGTAAGGAAAAAGCATGCCTCCCTGTGCT1320.12298976948735628No Hit
GCAAATGGGGCTTTCCGAGCCCGCTTGCACCTCGGCGATCCCCGACTCCCTT1310.12205802880942178No Hit
ACGTAGGGGTAATACCGTTCTAAGGAATGTTTGGGAGTGATTTGATTATTTT1300.12112628813148725No Hit
CGCTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1300.12112628813148725No Hit
ATTCTGGGGCAACAAAGAAAGACTAGAGATTTACTTAGTAACAAATGATGTG1300.12112628813148725No Hit
TTTAAAGGGCGATCTTTTACGGGGGCCCTCGAGGTGAAGGATTGGCCCTGAT1290.12019454745355272No Hit
CCTCGCGGGCTGTCTAGATCTGTGAGAACATTAGTCTTTTATAATACTTTAA1290.12019454745355272No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1290.12019454745355272No Hit
GTGCTGGGCGCTGGCTCCTGTTTCCAATTGATTGAGGGATGCCCCCAAGGAA1290.12019454745355272No Hit
AACTATGGGATCTGAAATTAGGAAGTTCAGAAATGTTAAAAATGTTAATTTT1280.11926280677561821No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1280.11926280677561821No Hit
TATCATGGGGTTATGTTTTTGTGCTTGTTCCCCACTTGATTCATCTGCAGAA1270.1183310660976837No Hit
AGTGCTGGGATTTGTGCAGGAAACCAGCCATTTAATTGTTCTAGAGTTTTAG1260.11739932541974918No Hit
TACCTAGGGCTTCTGCTGAGTACCACCGCAAAGCCCTGTGAGCGTCTACAGA1260.11739932541974918No Hit
AGACGGGAGATGGTCAGAATGCAGATAAATTGTGTGCATCTCAATCAGTAAA1260.11739932541974918No Hit
TGCCATGGGATTCCTTTAATACATATTTTTTGAGTACCTATTTTGTGCCAAA1260.11739932541974918No Hit
GCATCAGGGCGATCTCTCAGCGGGAGCTTCAGTGACCCAGGGTCTGAACTGC1250.11646758474181465No Hit
TCACACGGGTAGCGTGCACACATCTAGTTTATTCACAAAGGTATAATTTCCT1240.11553584406388015No Hit
TCATCAGGGGATCACATTTGCATTTTAGCTAGTACACTGTACCAGCAGTTAG1240.11553584406388015No Hit
GGCCTGGGGGTCATGATGTTCGTTGGCTTCCTGGGCTGCTACGGGGCCATCC1240.11553584406388015No Hit
TTCTGCGGGACCTCCTCCTGCAGCAGCGGCAGCGGCAGCAGCAGTTAGCAGC1240.11553584406388015No Hit
TCGCGGGGATCTAGTCGCGGGAGCACTAAATGCCCACTGGAGAAAGCGGGAA1230.11460410338594562No Hit
TAAATAGGGAATTTAGTATTCGGTAGTTAACATTCCTATTCACTATAATAAA1230.11460410338594562No Hit
AGTTCCGGGAGCTCCTCTGTCTGTACCACGTCGGGGGTTGGGACCAATCATT1230.11460410338594562No Hit
GTAATTGGGTAAAGGATTTGGAGATTAGGAGTGGATGTAGGGCAGAAGTCGG1230.11460410338594562No Hit
GTTATAGGGCTACATAAAGAAAGGCAAATGAATGGCTAATATTCATAAAGAA1220.1136723627080111No Hit
TCACCTGGGATATTCTAATTGTTAGCAACTCATTCAATTGGGATTTTTCTTG1220.1136723627080111No Hit
TGCCACGGATATTAGTGGTGGGTTAGGAAGTGGATTTAAAAGAAAAGAAATG1220.1136723627080111No Hit
GTCAGGGGGAAGGCAATAAAGAAAGACTTTCTCTTTGACGCTTGTGAATAAT1220.1136723627080111No Hit
GCTTCGGGGAAGTATGTAAAGGAAACATATTTTTAAAGAAGCTTAACAGTAA1210.11274062203007659No Hit
TTAGCCGGGCTGGCGTTGGGGTTGCAGGTGCAAAGTGACTTTGTTTATTCTT1210.11274062203007659No Hit
CAATACGGGTCAGTCTTCTCTGTGACGGCTAGTTTAGACATATCTGTGGACC1200.11180888135214208No Hit
ACGCGGGGTGGATGTTTGAATTATCTATGTATTTGGCCAAGGTTCATTATAT1200.11180888135214208No Hit
ATAATTGGGACTCAAGAGATTCTCCCCTAAATATTGATCTTTCTAAGTTTAC1200.11180888135214208No Hit
GTTAGGGGAACCTGCCATTATGTAATTTGGTTTCATTTTATTCTACCTGTTG1190.11087714067420755No Hit
TATAAAGGGGAATGGGATACGGACCAATGGAAGATTGAGAACTACATTAATG1190.11087714067420755No Hit
CAACCGGGGTATTTTCCAAAGATGGTCACAATAGAAGCCATTCCACATGCTC1180.10994539999627305No Hit
CAACTGGGAAGAGTATTAGGTTGCCGTCATCAGCCTTTGTAAATAAGTTAAA1180.10994539999627305No Hit
GGTCAAGGGGCTTTCTGCTCCGTGACGCACGGAGGCTTGGCAGCCACTCTTA1180.10994539999627305No Hit
TAAAAGTGGGCTTTCAGGTAATTTCTCTTTCTATCTGTGTATTATAAACCAC1180.10994539999627305No Hit
TGCGCAGGGGAAGAAAGAGCTACCTGTCACTTACCCGAGTTTTGAAATCCAG1170.10901365931833852No Hit
GAGCAAGGGTTAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA1170.10901365931833852No Hit
TCTGCTGGGGAGTTGCAGACAGCCCGGCGAACCGCGCAATGCGCTTCTTCTG1170.10901365931833852No Hit
TGCATAGGGAGTAGAACGGAGTGACCATTTTTTTGTATTGGTGTTCCCATGC1170.10901365931833852No Hit
ACAACCGGGCAACAAAAGTTACATATGCTACTAAGCTCAACAAAAAAGGAAC1170.10901365931833852No Hit
TCTTGCGGGATTCCTTCAAAACACTTCCATTTATAACTTGAAGATATAAAAA1170.10901365931833852No Hit
CATCATGGGACTTAATCTATAGGTTTATACATGTATGAGAAATGGGAAAAAA1160.108081918640404No Hit
CCTATTGGGAGGATAAATTAGTCATAGATAATAATATAAAATTCATCAGTGG1160.108081918640404No Hit
ACAGTGGGGTTCGTAGGCTGCAAGGCTATATGGTACAGCCTGTTGTTCGTAG1150.10715017796246948No Hit
TATCATGGGGAATGGATGATGTCACGACTTAAGATTAGATGATCTCTACTGA1150.10715017796246948No Hit
TTGTTTGGGTGTCTAATTTAATTTAAAGATCAATAGACAAATGAAACGTAAA1150.10715017796246948No Hit
TTATGCGGGAGGGCGCAGCTGCGCCCGCTTCAGCGCCTCCCGGGTCTGCGTG1150.10715017796246948No Hit
AGAGGGGATCCTCACCTTCATCCACAAAGGTAAACACTAGCCTGACTTCTAT1140.10621843728453498No Hit
TGCTTGGGGACCAGCTGTGTCCCTTGACAACAGGTCATGGTTCCTCTGAGGG1140.10621843728453498No Hit
CCTGCGGGACTGAGGCATGTACCAAAAACATAGAATTAAGTTTTAGCCTCTT1140.10621843728453498No Hit
GCTAATGGGAGTAAGGTCAGCTAAATAAGCTATCGGGCCCATACCCCGAAAA1140.10621843728453498No Hit
AGCAGGGGGGTATTAGTCCATTCTCACACTGATATAAAGATACTACCCAAGA1140.10621843728453498No Hit
ATTTTAGGGAAGCTGTGGCCAGCTCACGCCTGCTTCCTCCCTCCCTGCCCTG1130.10528669660660045No Hit
CACCTTGGGATTCTGCAGCTGTTGAATGAAATGTTCTGTTAATGTTTGTTAG1130.10528669660660045No Hit
TTCCAGGGGTAAGCAAAGAAATATTAAGGAACATTTTTTTTATGTTTTTGTA1130.10528669660660045No Hit
CTTATAGGGACTCTTTCTGGTAGAGGATTTCCAGTTTTCATCAGATTTCCAA1130.10528669660660045No Hit
AATAACGGGATCTGTTTTTGCATCTGTATAATGAAAGTATAATAACCTGTCT1130.10528669660660045No Hit
TCTTTGGGGAAATATGAATAGGTTTATATTGATTCATGGGTAACAGGTCCAT1130.10528669660660045No Hit
TGCATAGGGAATGAATGAATACAGAGTTCCTGAACTGAATGTTCAAAATGGA1130.10528669660660045No Hit
TATCAGGGGGTACAAAGACCAATGACACTATGAAGAAACTGCATCAACTAGT1130.10528669660660045No Hit
TATCGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.10435495592866592No Hit
ACACAGGGATGGATAGGGCCCAAAGCACATTTAACAGAAGAGAAACCTGATG1120.10435495592866592No Hit
GGGACGGGGAAGAGACATACATGACAAACCCACAGCAAACATACTGAATGGG1120.10435495592866592No Hit
GAAAACGGGATGGCTTTTAGTAATGCTTTGCAATTGGCTACGCAGATATAAA1120.10435495592866592No Hit
AATTCAGGGAACTCAAGATGGATTAAGGACTTAAATGTAAAACCTAAAATTA1110.10342321525073142No Hit
ACGACTGGGATCTTTTAAACTTATGTATTCACCAAGCCCAAAAATAGATTGT1110.10342321525073142No Hit
AACAGTGGGGAAGTAAAAAAACAATAATGCATATTTCCTCATATCATAAAAA1110.10342321525073142No Hit
AATAAAGGGTATTCTCTCACCAGTAGGACTTAAACTTTGTTTCTCCAGTGGC1110.10342321525073142No Hit
GTAGATGGGATGTACACATACACATAAATATTATTGCAATTAAGTGATCAAG1110.10342321525073142No Hit
ATAGTGGGGAAGTAATCCTCCCACCATGGCCTCCCAAAGTGCTGGGATTACA1100.10249147457279689No Hit
GGATTAGGAGTCATGACATTTCCCCATGAGAGTTCTAACAATTCTCTTCCAC1100.10249147457279689No Hit
TAAATTGGGCTCAGAAAGACTGCATTTCCCAGCCTTTACCACTTCTCAACCT1100.10249147457279689No Hit
CACGCAGGGACCAATAAAAAGATAGAAATTATTAAAAAGAACCAAATGAAAA1100.10249147457279689No Hit
GTAAAAGGGGTTCAGCTACGTCTTATTGAGCACTTCGTCCGTGCTGTACAAA1090.10155973389486238No Hit
TGAGGGGAGCATATATTTGAATGAATATAGATTGAAGAAATGTAATGTCACT1090.10155973389486238No Hit
CTACTAGGGTGGTGGGAGAAAAAGCCCTTCTCTGAGGATGTGAACTGGGGGT1090.10155973389486238No Hit
ATTATGGGGGAGGTGGGGGGCATGGCATCTTTCACTCAAATCATGATTTTGA1090.10155973389486238No Hit
TTTACTCGGGATTGTAAACACAGCTGCATGGGGAGGTCAGCTTGAGGTAAGT1090.10155973389486238No Hit
AACTAAGGGGGAATTTACAACTCAGAAAAATCTTCAAATCTGTCTGTAACAA1080.10062799321692788No Hit
AAATACGGGAGAAACTGTCATTACCTGCAGCAATTTCCAACTCATTCCTACA1080.10062799321692788No Hit
TAGAGGGAAGTGTTAAAAAAAAGTCTACATAAGAGTCTAGAAGTTTGTTGCC1080.10062799321692788No Hit
AGTTTCGGGAAGGGGAAAATCACATCCTCTTTGGAGATGATTATATTTTGAT1080.10062799321692788No Hit
CTTACCGGGCCTTCATGAATTGATTAATGTCATTATCATGAGAGTGGGTTGT1080.10062799321692788No Hit
TAGCTGGGGACTAATTAAAACAACAACAACAAATAGAATTCAGGATATAAGG1080.10062799321692788No Hit
ACATAGGGGCTGTATTTATTATATTCTGTATGCTAGAATTATTTATTGCATG1080.10062799321692788No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)