FastQCFastQC Report
Sat 31 Dec 2022
SRR5849444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107021
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC12581.1754702348137283No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT12101.1306192242643966Illumina Paired End PCR Primer 2 (95% over 21bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG1890.17660085403799253No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1880.17566645798488148No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1700.15884732902888218No Hit
CGCTTAGGGCTTACTAATGATCCCAGCACAGTGCTTTCTGTATATTATGTCT1570.14670018033843826No Hit
GACCATGGGAAAGAGATTACACTATAATTATGTTTAAAACCTTACTTGCACT1540.14389699217910504No Hit
CGCCGGGTAACAAACTGAGAGTCAAATTTTCTAAGTTTTTAAATATGCATTT1510.1410938040197718No Hit
TACCTAGGGGCCTGAAGAGGAGTACTCTCTACTTGTTGATACAGAAGGTGTT1500.14015940796666074No Hit
AAAGGTGGGAACCAAAAATGTGAAAGATCTCTACAAGAAAAACTACAAAACA1490.1392250119135497No Hit
GGCATAGGGGCTTTGTTGAAGTTATTGTGGAATGCAAAGTAGCACTGCAAAC1490.1392250119135497No Hit
TATAATGGGGAATGCAGAGGAAAAGTTCTTAAAGGAAATTAAAAGTGTTGCT1470.13735621980732754No Hit
TTTGACGGGTGAGTAAAGATTCTTAACTTTTTAAAGCTGTCGTTAGAAGTTT1440.13455303164799431No Hit
GCCTGCGGGGTGGCAAAGATGTTCCCTTGTAGGCCCCTTTCAGGTAACCGTC1430.13361863559488324No Hit
CAATACGGGTCAGTCTTCTCTGTGACGGCTAGTTTAGACATATCTGTGGACC1420.13268423954177216No Hit
TCTTGCGGGATTCCTTCAAAACACTTCCATTTATAACTTGAAGATATAAAAA1420.13268423954177216No Hit
CCTGCGGGACTGAGGCATGTACCAAAAACATAGAATTAAGTTTTAGCCTCTT1410.1317498434886611No Hit
AGTTTCGGGAAGGGGAAAATCACATCCTCTTTGGAGATGATTATATTTTGAT1380.1289466553293279No Hit
TCTTTGGGGAAATATGAATAGGTTTATATTGATTCATGGGTAACAGGTCCAT1360.12707786322310574No Hit
TAAATAGGGAATTTAGTATTCGGTAGTTAACATTCCTATTCACTATAATAAA1350.1261434671699947No Hit
GATTCTGGGAACTCCTGCAATTCACGGCATCCAAGATTCCTTACAGCCTCCG1350.1261434671699947No Hit
GCACAAGGGCAAAAAGTTAAATGTAGACACAGAAGATATTTTGAAAGCCCAC1350.1261434671699947No Hit
ACTTCAGGGCACCTCAGTAAAAACTGAAAAAATTTAAGGTGCATGCCAATTA1340.1252090711168836No Hit
CAGTTAGGGGGCCATTGGCAATTGCGCAGTTTTAGGCTTTTCTGTTCTTCCC1330.12427467506377253No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1330.12427467506377253No Hit
TTTCGCGGGAACATAGAGATAGGAAGTCATCTGACTCTGACAGCTCAGACTC1320.12334027901066147No Hit
GCTATGGGGAGACTTGCACTTTTTTAAAAAAAGGTTTCTGCATCGTGGAAGC1310.1224058829575504No Hit
TTCTCGGGGATGCCTACCTGGAAAATTCACCATTCTAAGACAAGGGACGTAG1310.1224058829575504No Hit
CCATGGGAGTCTTCCGGTCTGCAGCACGGAAAAGCACATAATCTTTCTTTGC1310.1224058829575504No Hit
AAGCCTGGGGGGCACAGCAAGACTTTGTCTTAATAAACAGACAAACAAACAA1300.1214714869044393No Hit
GGTTGTGGGGATATTGTCATAAGGACCGTGGGGGATTCAGATCCTTGACCGA1300.1214714869044393No Hit
AAAGCAGGGACGTGCTCCAAACGCTGAGTAGAGATTCTTGGATTGTGATGAA1300.1214714869044393No Hit
CATCATGGGACTTAATCTATAGGTTTATACATGTATGAGAAATGGGAAAAAA1290.12053709085132824No Hit
TGCCACGGATATTAGTGGTGGGTTAGGAAGTGGATTTAAAAGAAAAGAAATG1290.12053709085132824No Hit
GTGCTGGGCGCTGGCTCCTGTTTCCAATTGATTGAGGGATGCCCCCAAGGAA1290.12053709085132824No Hit
ACTGCTGGGTGGTAGAACTAGTGATAACAAAAACAGCAACGATTACTGCTAT1290.12053709085132824No Hit
AATAAAGGGTATTCTCTCACCAGTAGGACTTAAACTTTGTTTCTCCAGTGGC1280.11960269479821717No Hit
ACACATGGGAACCAACACCATTACCTAATTCCAGAACATTTTTATTGCCCTC1280.11960269479821717No Hit
TAAAAGTGGGCTTTCAGGTAATTTCTCTTTCTATCTGTGTATTATAAACCAC1280.11960269479821717No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1280.11960269479821717No Hit
TTCCTAGGGGAGAAATTAATGGCTAATTAATTTGCCCAAGGACAAAGAAAGT1270.1186682987451061No Hit
CGCGCGGGGGGGTTTGAGGGAAGAGAGCTTTTCGTAGGAAGGGTGAAATCCC1270.1186682987451061No Hit
ATTGAGGGATTGAAAATACAGCTTTTACGTGGATGCAGGACTGCTATAAGAA1260.11773390269199503No Hit
TATCATGGGGTTATGTTTTTGTGCTTGTTCCCCACTTGATTCATCTGCAGAA1260.11773390269199503No Hit
CGCTTTGGGGGGGTAGCTATCCTTACATCAGACAAGCAAACTTTAAAGCAAC1260.11773390269199503No Hit
TGCGCAGGGGAAGAAAGAGCTACCTGTCACTTACCCGAGTTTTGAAATCCAG1250.11679950663888396No Hit
TCTGCTGGGGAGTTGCAGACAGCCCGGCGAACCGCGCAATGCGCTTCTTCTG1250.11679950663888396No Hit
TCGCGGGGATCTAGTCGCGGGAGCACTAAATGCCCACTGGAGAAAGCGGGAA1240.1158651105857729No Hit
TACAAGGGGTTCCTGTGAAATACTAGAAAAGTTGTCAAATCTTCATGAATTG1240.1158651105857729No Hit
GCGTTGGGGTGTCCTCAATTCTGCAGCTTTTTTCATACTTTTGAATGTTTCT1240.1158651105857729No Hit
TTCAGTGGGCTCCCAATTCCTTTGGATAACTTCATTGCCTAGTAGATTACAG1240.1158651105857729No Hit
TGACATGGGGGAAGGCATGTAACCACCTAAACCATCTCCGAGAACATCAGAG1240.1158651105857729No Hit
TATCATGGGGAATGGATGATGTCACGACTTAAGATTAGATGATCTCTACTGA1230.11493071453266182No Hit
GTAGATGGGATGTACACATACACATAAATATTATTGCAATTAAGTGATCAAG1230.11493071453266182No Hit
AACTATGGGATCTGAAATTAGGAAGTTCAGAAATGTTAAAAATGTTAATTTT1230.11493071453266182No Hit
CACAAGGGGATATACAACTTGCAGTCATTGTAAATATGCGTGAGTTAGGGGT1230.11493071453266182No Hit
ATCTTTGGGACATAATAATGATCCATCCTGCTTTGCTGAAAGCATTTCTCTA1220.11399631847955073No Hit
GCCATGGGGGGCTACCGTGTCAACGAGGCTGCTTTACAACAGCTTTATTTAT1210.11306192242643968No Hit
ACTACAGGGTGCAACACAATGTTTCCAACTCTCTAACATGTTTAACCCTCAA1200.1121275263733286No Hit
TTAGCCGGGCTGGCGTTGGGGTTGCAGGTGCAAAGTGACTTTGTTTATTCTT1200.1121275263733286No Hit
TGACCTGGGATAGATGCAAGAAAAAAATGTTTATGAATTGCATTTGTATATT1190.11119313032021752No Hit
GTCAGGGGGAAGGCAATAAAGAAAGACTTTCTCTTTGACGCTTGTGAATAAT1190.11119313032021752No Hit
ACAGTGGGGTTCGTAGGCTGCAAGGCTATATGGTACAGCCTGTTGTTCGTAG1180.11025873426710646No Hit
CAAAGTGGGACCTGCCATTCACTAATCCTTAAACCATGTCTATACATTTATT1180.11025873426710646No Hit
CCTATTGGGAGGATAAATTAGTCATAGATAATAATATAAAATTCATCAGTGG1180.11025873426710646No Hit
ACGTAGGGGTAATACCGTTCTAAGGAATGTTTGGGAGTGATTTGATTATTTT1170.10932433821399538No Hit
GTTCAGGGGATCTAGCGAAGGGATCCTTGATATTCTGACACACAAAATTTAC1170.10932433821399538No Hit
CACGCAGGGACCAATAAAAAGATAGAAATTATTAAAAAGAACCAAATGAAAA1170.10932433821399538No Hit
AATTCAGGGAACTCAAGATGGATTAAGGACTTAAATGTAAAACCTAAAATTA1160.10838994216088431No Hit
TGCTGCGGGGTGGCCCAGGGGTTGGGGACCCCTGCCTTACACCACATGGCGT1160.10838994216088431No Hit
TTGGACGGGATATTTAAAAGTAATCTGTAGATTACTTACAATACCTAATACA1160.10838994216088431No Hit
AGACGGGAGATGGTCAGAATGCAGATAAATTGTGTGCATCTCAATCAGTAAA1160.10838994216088431No Hit
ACGCGGGGTGGATGTTTGAATTATCTATGTATTTGGCCAAGGTTCATTATAT1160.10838994216088431No Hit
AGCTGGGATCCTGAAAAATGTTAAGTAAGGAAAAAGCATGCCTCCCTGTGCT1160.10838994216088431No Hit
ATCATTGGGGGGGCTGACAGTGGGATTTCCTTTCCTGTTCTTTATGGGATGG1160.10838994216088431No Hit
TTAACAGGGGTAATGTTACACAAGTGAAAGAAATACATTTTTAAAACTTTTT1160.10838994216088431No Hit
CGTGGCGGGAAGTCAATGAATGAATAAACAGGGATTAGCACCAGTCTCAGCA1150.10745554610777325No Hit
GTTATAGGGCTACATAAAGAAAGGCAAATGAATGGCTAATATTCATAAAGAA1150.10745554610777325No Hit
GTAATTGGGTAAAGGATTTGGAGATTAGGAGTGGATGTAGGGCAGAAGTCGG1150.10745554610777325No Hit
AGAGAAGGGATATATTTTAATTATATTTGACAGGTTTTGCATCTAAATTGAC1150.10745554610777325No Hit
ACATAGGGGCTGTATTTATTATATTCTGTATGCTAGAATTATTTATTGCATG1150.10745554610777325No Hit
TCAAGCGGAGAACTCCTTTGAAAATATTTTATTTCTGTTTGTAAAGGAACTT1140.10652115005466217No Hit
TTCTGCGGGACCTCCTCCTGCAGCAGCGGCAGCGGCAGCAGCAGTTAGCAGC1140.10652115005466217No Hit
GCTAAAGGGTCTAAAATATGTAGAGCAGGTATATAAATTCCACTTTCCTTTG1130.10558675400155108No Hit
ATAAGCGGGGGATCATTAAAGCCAGTTTCATCAAACAGTTCAACAGAGAGCA1130.10558675400155108No Hit
TATCAGGGGGTACAAAGACCAATGACACTATGAAGAAACTGCATCAACTAGT1130.10558675400155108No Hit
ATTTTAGGGAAGCTGTGGCCAGCTCACGCCTGCTTCCTCCCTCCCTGCCCTG1120.10465235794844002No Hit
GGTCAAGGGGCTTTCTGCTCCGTGACGCACGGAGGCTTGGCAGCCACTCTTA1120.10465235794844002No Hit
AGTGCTGGGATTTGTGCAGGAAACCAGCCATTTAATTGTTCTAGAGTTTTAG1110.10371796189532895No Hit
AGAGGGGATCCTCACCTTCATCCACAAAGGTAAACACTAGCCTGACTTCTAT1110.10371796189532895No Hit
ACAATGGGGTACAAAAGAATACCTAGTGAGGCCAGGCGCAGTGGCTCACATC1110.10371796189532895No Hit
ATTTCAGGGACAAAATAGCCCTTGAAAATTCCTTTGCTGCCCATAAAATAGC1110.10371796189532895No Hit
GCATGTGGGGGATAGATTTACATTTCTAGGCATACTCTCAGGTCCTCTATGT1100.10278356584221789No Hit
GAAAACGGGATGGCTTTTAGTAATGCTTTGCAATTGGCTACGCAGATATAAA1100.10278356584221789No Hit
TAGCTGGGGACTAATTAAAACAACAACAACAAATAGAATTCAGGATATAAGG1100.10278356584221789No Hit
AATCCTGGGGGCCCTTTACAGAAAAAGTTTGCTGGCCTTTATCTAATGTGTG1100.10278356584221789No Hit
ACGACTGGGATCTTTTAAACTTATGTATTCACCAAGCCCAAAAATAGATTGT1090.10184916978910681No Hit
TACGGCGGGAAGGTCATGATAATTTTCTTCTGTGCTACCTTCTAGAAGCTTT1090.10184916978910681No Hit
TTGCTTCGGGAACTCATGTAGGTTTTTATTGTGTGCTAACAAAATTAGGCCT1090.10184916978910681No Hit
TATACCGGGGAAAGTTTGATTGTGTTAATATATGTAAAATACTTTAAAATAG1090.10184916978910681No Hit
ACTCGCGGGGACGCTCTTCCGATCTATTCTAGGGGAAGTTGCAGTTGTTGAC1090.10184916978910681No Hit
TGCTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1090.10184916978910681No Hit
ACCGCCGGGGCATACTGAAAGGTCCGAAAAGGGCATCCACGGCAGCTTGAGC1090.10184916978910681No Hit
GCATCAGGGCGATCTCTCAGCGGGAGCTTCAGTGACCCAGGGTCTGAACTGC1090.10184916978910681No Hit
GAGCAAGGGTTAGATAGATAGATAGATAGATAGATAGATAGATAGATAGATA1080.10091477373599574No Hit
TCTTAAGGGTAGTACTTAAAAACTAGTTTTGTATACAGAAATACAAAAGAAA1080.10091477373599574No Hit
GGCCTGGGGGTCATGATGTTCGTTGGCTTCCTGGGCTGCTACGGGGCCATCC1080.10091477373599574No Hit
GCTTCGGGGAAGTATGTAAAGGAAACATATTTTTAAAGAAGCTTAACAGTAA1080.10091477373599574No Hit
GGTTGCGGGAGCTTGATAGAAGAATGGAAATGACAGAACAAATAGTTCATAA1080.10091477373599574No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)