FastQCFastQC Report
Sat 31 Dec 2022
SRR5849443.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849443.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94138
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC14571.5477278038624147No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT14241.5126728844887294Illumina Paired End PCR Primer 2 (95% over 21bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2560.2719411927170749No Hit
ATAACTGGGGATTCCTCCAGCAGTCAAGCATTTTCCAAAGGGCCCTGTCTTT1950.20714270538995944No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1890.20076908368565297No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1790.19014638084514224No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1700.18058594828868257No Hit
TTGAAAGGGCTGTCATGCCTATTGCCTAGCTGCCAGATTAACCTTGCCTTGA1680.17846140772058042No Hit
GCTATTGGGAAGTATGGTCAAGGAGTGAACTATTTTCTTTTGGAAAAACTTT1650.17527459686842722No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1610.1710255157322229No Hit
TAGGTCAGGGGTTATTGGGATTTTGATCAGTTATTATAACTGCCATAGGTGG1590.16890097516412075No Hit
TCAGCCGGGGCTTCTCTCTTAGAATAAATGACATTAACATATGAAAAAACAG1560.16571416431196753No Hit
TTGCTTGGGGCGTCCTGTCTGTCTCCCTATAAGGACAGGGCTTGGCTTTTTG1520.16146508317576325No Hit
CAGTCAGGGCGATCTTAGGCTGGGAACTGCTCTGAGTATGCATTTGTTTTCA1490.15827827232361003No Hit
ACTCTTGGGGGACTGGCGGCAGGCTCGCCGCGGCGCGGAGTCCCGGCTGCGG1480.15721600203955896No Hit
GAGCAAGGGCTCGAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAAT1470.15615373175550787No Hit
AGCCTGGGAAGTCACATTATGCACAGATATCTTATACATGTAGAATTACGCA1470.15615373175550787No Hit
TGCTTACGGGACTAGTGACTAGTTTACTAACTAGGTCATTCAAGGAAGTCAA1440.15296692090335465No Hit
CACTGTGGGAATTCAAATTGTTACTATATTCATGACAAAGTAAGACAATTTA1410.14978011005120143No Hit
CACTTCGGGTACAGGTTTTTGTGTGGACATAAATGTTCAACTTTTTCAGTAT1410.14978011005120143No Hit
GTCACAGGGCAAGAGGCTTACAATTATTCTGGAATTTGTGTTTGCTTATTTG1410.14978011005120143No Hit
CGTTTTGGGGAAGTTTTGTATATATGTCCAAAGACTTAAAAGGGAATTATGT1400.14871783976715036No Hit
GCGCATGGGATGAGTCAAAATTTTATGGATTGTAAACTAGGCTTTATCTGGT1380.1465932991990482No Hit
TGTTTGGGGTACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAA1380.1465932991990482No Hit
CAAACCGGGATTACTCTGCATTATAATGAAGCCAACAGTCATATCTTCTGAT1370.14553102891499714No Hit
GTCATAGGGATTTTAACAGAGATCAGCACTTGGCAACTTGACATGTTGGGCA1350.14340648834689498No Hit
GTTAAGGGCAGGCAACATGAATCTGGGGATTCAGTTTCCAATACATGAAATT1350.14340648834689498No Hit
TATCGAAGGGATGACAAAATTATTTATGATAATTGGATGGCTGCAATTGAGG1330.14128194777879285No Hit
CATATCGGGATATCTTGAAGTTGTTGTTTTTTTTTTTTTTTTTTTTTTTTTT1320.14021967749474176No Hit
ATACTTTTGGGTAAACATGTTGTTATTGCATTATGCTTCTAGAGGAGACATC1310.1391574072106907No Hit
TCATAAGGGTAAATGTTAACGGCTATTTTAAATTCTATTCATTGTAATGGCA1300.1380951369266396No Hit
TCGAATGGAACAAAAAACATTATCTGTAAATATGAAATTAAACTATAAGAAT1290.13703286664258854No Hit
GATATGGGAGGACAGCTAAGAAATTAATATTAATATCAAAATTATTGATAAT1290.13703286664258854No Hit
TCTTTAGGGCTGATAATTTGAATCCTGCCATGAAATTTCCCAAGTTCACTTT1290.13703286664258854No Hit
AAATGAGAGGAGTGATATCATTTGCAGCACAAAGTTGCTAATTTTGATGCAG1290.13703286664258854No Hit
AAAACTGGGAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACC1280.13597059635853745No Hit
GGTTACGGGCATTCTAGACTCTTCCAGGCAGCTTTCTGTTTGTTCTCTAACA1280.13597059635853745No Hit
GTTGTTGGGATTGATGGATATTTAGCTTAATTCCATATCTTGGTTATTGTGA1280.13597059635853745No Hit
TCAATAGGGGACATGCTTTTTATTCACTGACTAGTATTCACTTTTTTACAAC1280.13597059635853745No Hit
ATGTACGGGGAGGTACTGGGAGTTAGGACTTCAACAAACAAGTTTGGGATGG1270.13490832607448638No Hit
CCAAAAGGGACTGTTTAGTGGGTAAAGAGTTTCAGTTTTTCAAGGGAAAGAT1270.13490832607448638No Hit
ATTTTGGGGAGATAAATAGATGTCTCCTTTATAAGTAGCAGAAGGATATTCG1260.13384605579043532No Hit
GGTCTAGGGAGAACAAAGGATTGACATCTTTGACAATAATTAGACTGGCATA1260.13384605579043532No Hit
TTTCCCGGGAAGTTCTGAAGAGTATGCATTTAAAAGTAAAAGTTTTCCCTTA1260.13384605579043532No Hit
CTCAGCGGGAGATCTCATACACCAGAATTCAGATCATGAATGACTGACAGAA1260.13384605579043532No Hit
AAATCTGGGATGTCTTGTGTGCGTGTCCTTAAACTTCCAATCTTACTTTGTC1250.13278378550638426No Hit
TGTCAGGGCAAGAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTGC1250.13278378550638426No Hit
CTTGGTGGGCTTACTGACTAGATTCATTTTGCATTAGCAGGGGATGGCGAAG1240.13172151522233316No Hit
CTTTGAGGGTAAGTAATTAGTAAAAGAGGATCACTCCCTTCACCAGGGAGAA1220.12959697465423103No Hit
TTCCTGGGGAGCTTTAAGTCTAAATACAAAGCTGTTCTGTGTGAGAATTTTT1200.12747243408612888No Hit
ACGCTTGGGCTGGTCAGCAGTGTCACCTGCCCCAGAGAGGCCAAGAGTGGGA1200.12747243408612888No Hit
TCTAGCGGGACCTTGAAGAAGTATGACGGTCGAACCAAACCCATTCCAAAGA1200.12747243408612888No Hit
CGTAAAGGGAAATACAACATGTACACAGGTTTTCATAGCATTATTCATAACA1180.12534789351802675No Hit
AAAGCGGGGATAAGGAGAAATGTCTGTTTTGAGGAAAGGGCTTTGAATTCCC1180.12534789351802675No Hit
ACTAATTGAGGGTCTATTTGATTCAAATGGTATGTTTTTTCACATTTCTCAT1180.12534789351802675No Hit
CTTATGGGGTCAGGATCATGACCAAATTTCTCCCAACCAAAAACATTTCCTT1180.12534789351802675No Hit
ATCTAAGGGATAATCACCAAGTTTAAATAATCAGTGTGGCATTTTACAAATG1170.12428562323397564No Hit
TCCAACCGGGCATGCGGAAAGTGACAAAGTTGGCCATGAACAAAAAGTTTAA1170.12428562323397564No Hit
AGCAGTGGGATTTTCATGAAGTTTTTAAGAAATATTTAATAGAAGGTGCTGT1170.12428562323397564No Hit
TGTAATGGGATGAGTTCCTGAGACATGCTCTTTTGGGGGCTGGGGCTTTAGG1160.12322335294992458No Hit
AATGACGGGAAGAGGCCCTAAGACTGGTAGAAATTAAAAGGATTTCAAAAAC1160.12322335294992458No Hit
AATAAAGGGATTCATATGAGAAACAAAACCCCAAAAGCACAACTTTAAATAA1150.12216108266587351No Hit
ACTTTGGGGCCCAGAGCCAAGGACTTGTACCCTGTGTAATGTTTGTGCTGAA1150.12216108266587351No Hit
TACCTTGGGATTTATGATATATTACTACTTATAATACTGTGGCTAGTCTCTA1150.12216108266587351No Hit
TTTTAAGGGGATGAGACATAATTAAGGGTCATCTACAATGGACAATTTTCAA1140.12109881238182242No Hit
GGCTTAGGGCATGCTCTGGTCTTTGAAAGATTAATAAAAGGGGGGAAATTTT1140.12109881238182242No Hit
GACGCCGGAAGGCTATTTTGAACTTCTGAATAGAATTACACTTACAATAAAA1140.12109881238182242No Hit
GATTCAGGGCTGTGTCGCAGCTGGAGAGGCAGGGTCAAGCACGCACGAGAAG1140.12109881238182242No Hit
GCCAAGGGGAATTTATACCAGTGGTAAAATGGTACCACTTAGTCTGATACTT1140.12109881238182242No Hit
ATATGTGGGATTCACATTGACCGCAACTCACACATTCCCCCACCAGCTGCAG1130.12003654209777136No Hit
ACTTTAGGGTTTTAGAAAAGGTGTCAGGCTTACTCAGCAGGACAGAGGCTGA1120.11897427181372028No Hit
TCTGCCGGGATGATTAGTACTGAGTGTCAACTTGATTGGATTGAAGGATGCA1120.11897427181372028No Hit
GTAGTAGGGGGATGGTGACTTTTATTTCCAAGCACAGCATAATCCACAATAA1110.11791200152966921No Hit
TAATTGGGGGTCCAATTTAAAAATGGAACTAGACATACATAGAGGCATTAAC1100.11684973124561812No Hit
GCATAGGGGACCATTTTAAAACTATAGCTGGATCTAATGACAAATAAATTAT1100.11684973124561812No Hit
GCTACAGGGACTAATGATGCATTATATACACTTGATTATTAATTTTGCTTTT1100.11684973124561812No Hit
TTTCAGGGATGTCAATGATAGCACGTAAGTCAGAAGGATGAATGGAGTTTCT1100.11684973124561812No Hit
CAACGGGGGATAAATTGTCGGTACATTCAGCTCTTCTAGGTGAGCAATACTG1100.11684973124561812No Hit
ACTTGTGGGAGGGACAGAACTCAATTTATATTATTAATAGCTTGCACTTAAA1090.11578746096156706No Hit
GCTTTGGGGATCCTACAACCAGCCAAATGCACTTTTGTGAAAATGGGGTGTG1090.11578746096156706No Hit
GTTTCTGGGGTTTGATGAAAAGAGATATTTTGCTTAATTTACTTGAATTTAT1090.11578746096156706No Hit
TACTGCGGGACAGCATTTAATAACTGCATTGCCTATTTTCAACTCAGGTTAC1090.11578746096156706No Hit
AAGCTGGGGATTTTAGTATCTGGTAGAGCTAATATTTCCATCATCTTTATTC1090.11578746096156706No Hit
AGCCAGGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTG1090.11578746096156706No Hit
TGAAAAGGGCTTGACAAAATGTGTTCTGCCTCACATGATCGAGAGGAAGCAA1080.11472519067751599No Hit
CCAACAGGGGTTACTGTCTGTTTATTGATAACAATCTCTGTGTTATAGATGA1080.11472519067751599No Hit
TTTTACGGGCCCAGATTGTAGGTAGAAGTGTGTTTGCAAAGGCCAGAGGAGC1080.11472519067751599No Hit
AGGCTGGGGCAAACATCCTTGAGCGCACTCACACAACCTGGAGGATGTAGCT1080.11472519067751599No Hit
AATTGTGGGGAGCTTTTCTGTACAGCAAAATAAACTATCAACAGATTAAACA1080.11472519067751599No Hit
GCTGTGGGGAAATAAAAGAGGACACAAACAAATGGAAGAACATTCCATGCTC1080.11472519067751599No Hit
CATTGAGGGCTTGGTCTGCAGTCTGGAAGCTTGTCTGCTCTATAGAAATGAA1080.11472519067751599No Hit
TGGACTTTGTGGAACAGAAATGTGAAGTTTTTGATGATGAAGAAGAAAGCAA1070.11366292039346493No Hit
GCTGCTGGGAACGTGGCACTGGTAGGTTGGGACACCAGGGAAGAAGATCAAC1070.11366292039346493No Hit
TTTAAAGGGCGGTATTCCTGGTCGAAGCCCAGGAGCCTTCCCTTTTAACAAG1060.11260065010941384No Hit
CAGCAAGGGATGGATGTCTGGGCAATCATAGCACTTGCCATTTAAAAACATG1060.11260065010941384No Hit
TTGCCCGGGATTGAGCAGAATGACACTTAAGCCTTAGATTTTGTGTAAATGC1060.11260065010941384No Hit
CCACTTTGGTGAGCAGGGGAGCATAAAATTCATGGGATAGGGACTTTGTTTA1060.11260065010941384No Hit
ACAGATGGGGAAGCCCAAATTAAAAGTCACAGTTGCGGCCAGGTGTGGTGGC1060.11260065010941384No Hit
AGCATCGGGGGTGTCTTTCGGCAACTTACACACATTGAAAATGAGCTATTGT1050.11153837982536277No Hit
GTTTTGGGCACCATAGCAAGAAGGAAATTATTGATTTATGTCCCCAACATCA1050.11153837982536277No Hit
AAATAGGGATTTCTGTTTAGTTTTCTCCAAAAATAAAACACATTTCATATTT1050.11153837982536277No Hit
GCTTAGGGAGACTTAAAACCATTTACTCCCAAATTTTTGAGTATGCCCTCAC1050.11153837982536277No Hit
TTGCTCGGGAAGTTTTTCTATTAAGCAAGTTCTAGGTGATGTAGCAGAGGCA1050.11153837982536277No Hit
GTATATGGGAGAAAAACAAATTGTGTTTTCAAAAGGTGCTAACAAAATATCT1040.11047610954131169No Hit
TGTCCCGGGGGGGCGCCCACGTCCTCTGTCTCACCAACAGACACAGACATTT1040.11047610954131169No Hit
TTCAGTGGGATGTGGGTTATGCATCCATAGATTCAACCGACTACACATCAAA1030.10941383925726061No Hit
ACAAAAGGGAAAGACGTCAGTTCTAAGTTGGTTGCAGGGTTCTGGGCAAAGG1030.10941383925726061No Hit
AGCAAAGGGAAAGTTGAAATGCTAACACTACGTTATTTGAAGACTTTATTAT1030.10941383925726061No Hit
TGTCGGTGGGATATAGGTCATTGTTAACAAACAAACATCCCTGAAAACCTCT1020.10835156897320954No Hit
AAACTTGGGATCACTGGTAAACCATGGTAAACAGTTTTGTAGTTTTCTAAAT1020.10835156897320954No Hit
AAAGGGGGACATATTTTGAAACGGCCCTGCAAAGCTGTCCTTTGCGGGAGAA1020.10835156897320954No Hit
GTCCCAGGGAATAAGACAAATGCAGTCTACATTTCAAAATTGGTCATTTTTG1020.10835156897320954No Hit
ACCTGAGGGGATCTCTGCTGGAGGTTGTCAAAAGAACACAGCCAAGCACACA1020.10835156897320954No Hit
AGGCCTGGGGAACGTGGGCCCTCATCTCAGATCTGCCCCCAGTATGTTTAGG1020.10835156897320954No Hit
TTACATGGGGTCTGCACCAGCTAAGTACATGCCTTACATGCATTGTTAATTC1020.10835156897320954No Hit
GATCTAAATTAGGGGTTGGGAAATATGTTTTGGTACTGGGAAAGGAGTTCTA1010.10728929868915847No Hit
ATCACAGGGCCTCTTCCACACATGTATGTGATAAAGGATCTTGTTCCCGATT1010.10728929868915847No Hit
GTGCCGGGGTATTTTACAAAGAAATTTGAACAAATTTAATTGAATCTTTTGT1010.10728929868915847No Hit
ATGCTCGGGAGCCCCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTA1010.10728929868915847No Hit
CAGAATGGGATGGGACTGGTATATAATTAGTTATAGTTGTGGAAAACAAAAT1010.10728929868915847No Hit
GCTGCTGGGGGAACCAGGTCCCACCTCCCCACAAGGCAAATCTAGACCCATT1010.10728929868915847No Hit
GCAACCGGGATAGGGAGGTGGTGACATTCTGGATACTTCCACCATGGGGCGT1000.10622702840510741No Hit
CAGTGCGGGAATTGTATTGGTTTTTAAAAATAATTAAAATGGCAACACTTTG1000.10622702840510741No Hit
GTCAAAGGGAGATTTGGATAAAATTTTAACTCAACATCTTGATTTGGAGCCT1000.10622702840510741No Hit
GTGTCCGGGACATACACAGCCTTCCAGCTTCTATTATGATGCATTGTACTGT990.10516475812105631No Hit
TAGCAAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC990.10516475812105631No Hit
CTGCACATGGGGAGAAGGATTTGTAAACCCCGGAGCGAGGTTCTGCTTACCC990.10516475812105631No Hit
CGGGGTGGGATATCCTGGCAGTTCGGCCACATCCTATGTGGTCTACATTTAG990.10516475812105631No Hit
TAACTGGGGGGATGGTGACAGTTCTACAACAGATGCTTCTCAACTAGGAATC990.10516475812105631No Hit
ACGCGGGCTACCTCAAAGGGACACTTAGTATGCCTAAAATTTATTCACTTAG990.10516475812105631No Hit
AAGTTTGGGAGGTACCGTTTGTGTAACATTACTCATTTCTGTATATTCCTTT990.10516475812105631No Hit
CTGATCGGGATCAATGTCAATATCATATAGTACAATATGATATTATAGTTGC980.10410248783700525No Hit
ATTTATGGGATTCAGACCTCAGGTAGAGAAAGCTGTGCATGAGTTTTTGGCC980.10410248783700525No Hit
TCAACTGGGCTCTTATCACTAAGAAAGAAAGGAAAGAAGGAGATGAATAGAA980.10410248783700525No Hit
GACGCAGGGATTGGTGCCCAGGGAGAACCCCTTGGATATCGCCAGGATGATC980.10410248783700525No Hit
TTACCTGGGGCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC970.10304021755295417No Hit
GGTACTGGGCACTAGCTGTTAGTAGGTTAGTTTTTGAAGATTTTAAAGAATT970.10304021755295417No Hit
GCCACTGGGTTGTTTATTTATGTGAATCAGTCCTTTGCTCCTTCCCCAGACC960.10197794726890311No Hit
CGCTGCGGGGAGTTGATTAGCAGGAAAGTCAAAAGTAGAGCTCATCTCTTGG960.10197794726890311No Hit
GAGTAACGGGGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAGAAA960.10197794726890311No Hit
GCTAAAGGGGGTTAATAGGTGCACTAATTAATTAATAGATGTATTAATTTTT950.10091567698485202No Hit
TGATATGGGAAACTTGATCTTGTCCAGGAAAGCTTTGTACCAAACAGTGAAG950.10091567698485202No Hit
ATGCCAGGGCTTTCAAAGGCAGGACATACTTATCATTGACTTTTAAGTTGCA950.10091567698485202No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)