FastQCFastQC Report
Sat 31 Dec 2022
SRR5849441.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849441.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93390
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC13371.4316307955883927No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT12921.3834457650712066Illumina Paired End PCR Primer 2 (95% over 21bp)
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2190.23450048185030517No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC1890.20237712817218118No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1800.19274012206874397No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1700.1820323375093693No Hit
TTGAAAGGGCTGTCATGCCTATTGCCTAGCTGCCAGATTAACCTTGCCTTGA1700.1820323375093693No Hit
ATAACTGGGGATTCCTCCAGCAGTCAAGCATTTTCCAAAGGGCCCTGTCTTT1700.1820323375093693No Hit
TCATAAGGGTAAATGTTAACGGCTATTTTAAATTCTATTCATTGTAATGGCA1690.18096155905343184No Hit
TTGCTTGGGGCGTCCTGTCTGTCTCCCTATAAGGACAGGGCTTGGCTTTTTG1680.1798907805974944No Hit
CGTTTTGGGGAAGTTTTGTATATATGTCCAAAGACTTAAAAGGGAATTATGT1670.1788200021415569No Hit
TCAGCCGGGGCTTCTCTCTTAGAATAAATGACATTAACATATGAAAAAACAG1600.17132455294999466No Hit
TAGGTCAGGGGTTATTGGGATTTTGATCAGTTATTATAACTGCCATAGGTGG1600.17132455294999466No Hit
AGCCTGGGAAGTCACATTATGCACAGATATCTTATACATGTAGAATTACGCA1600.17132455294999466No Hit
GCTATTGGGAAGTATGGTCAAGGAGTGAACTATTTTCTTTTGGAAAAACTTT1570.16811221758218226No Hit
AAAACTGGGAACACCTCCTCTGCTTATCGTATTTTATCAACAGCACTCTACC1480.15847521147874505No Hit
ATACTTTTGGGTAAACATGTTGTTATTGCATTATGCTTCTAGAGGAGACATC1450.15526287611093265No Hit
CACTTCGGGTACAGGTTTTTGTGTGGACATAAATGTTCAACTTTTTCAGTAT1440.15419209765499517No Hit
CATATCGGGATATCTTGAAGTTGTTGTTTTTTTTTTTTTTTTTTTTTTTTTT1430.15312131919905772No Hit
AAATGAGAGGAGTGATATCATTTGCAGCACAAAGTTGCTAATTTTGATGCAG1370.14669664846343292No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1370.14669664846343292No Hit
GATATGGGAGGACAGCTAAGAAATTAATATTAATATCAAAATTATTGATAAT1360.14562587000749547No Hit
TAGTAAGGGAACAGAAAACCAGCATATTTAATCAAAGCAAGAAGTAATCGCT1360.14562587000749547No Hit
TACCTTGGGATTTATGATATATTACTACTTATAATACTGTGGCTAGTCTCTA1360.14562587000749547No Hit
TTTCAGGGATGTCAATGATAGCACGTAAGTCAGAAGGATGAATGGAGTTTCT1350.144555091551558No Hit
GGCTTAGGGCATGCTCTGGTCTTTGAAAGATTAATAAAAGGGGGGAAATTTT1340.1434843130956205No Hit
GGTTACGGGCATTCTAGACTCTTCCAGGCAGCTTTCTGTTTGTTCTCTAACA1340.1434843130956205No Hit
ATTTTGGGGAGATAAATAGATGTCTCCTTTATAAGTAGCAGAAGGATATTCG1300.13920119927187066No Hit
TCGAATGGAACAAAAAACATTATCTGTAAATATGAAATTAAACTATAAGAAT1300.13920119927187066No Hit
CAGTCAGGGCGATCTTAGGCTGGGAACTGCTCTGAGTATGCATTTGTTTTCA1300.13920119927187066No Hit
TTCCTGGGGAGCTTTAAGTCTAAATACAAAGCTGTTCTGTGTGAGAATTTTT1300.13920119927187066No Hit
TGCATGGGAATATCAGATGCCTTGTAAATTATGTCTTTAACGTTTTCTTATA1300.13920119927187066No Hit
ACTTTAGGGTTTTAGAAAAGGTGTCAGGCTTACTCAGCAGGACAGAGGCTGA1290.13813042081593319No Hit
CTTGGTGGGCTTACTGACTAGATTCATTTTGCATTAGCAGGGGATGGCGAAG1290.13813042081593319No Hit
CTTTGAGGGTAAGTAATTAGTAAAAGAGGATCACTCCCTTCACCAGGGAGAA1280.1370596423599957No Hit
GATCTAAATTAGGGGTTGGGAAATATGTTTTGGTACTGGGAAAGGAGTTCTA1270.13598886390405823No Hit
GCTGTGGGGAAATAAAAGAGGACACAAACAAATGGAAGAACATTCCATGCTC1270.13598886390405823No Hit
AAGCTGGGGATTTTAGTATCTGGTAGAGCTAATATTTCCATCATCTTTATTC1270.13598886390405823No Hit
GCGCATGGGATGAGTCAAAATTTTATGGATTGTAAACTAGGCTTTATCTGGT1250.13384730699218333No Hit
TCTAGCGGGACCTTGAAGAAGTATGACGGTCGAACCAAACCCATTCCAAAGA1250.13384730699218333No Hit
GATTCAGGGCTGTGTCGCAGCTGGAGAGGCAGGGTCAAGCACGCACGAGAAG1250.13384730699218333No Hit
TGCTTACGGGACTAGTGACTAGTTTACTAACTAGGTCATTCAAGGAAGTCAA1240.13277652853624586No Hit
ACGCTTGGGCTGGTCAGCAGTGTCACCTGCCCCAGAGAGGCCAAGAGTGGGA1240.13277652853624586No Hit
AGGCCTGGGGAACGTGGGCCCTCATCTCAGATCTGCCCCCAGTATGTTTAGG1240.13277652853624586No Hit
TCCAACCGGGCATGCGGAAAGTGACAAAGTTGGCCATGAACAAAAAGTTTAA1240.13277652853624586No Hit
CACTGTGGGAATTCAAATTGTTACTATATTCATGACAAAGTAAGACAATTTA1230.13170575008030838No Hit
AAAGCGGGGATAAGGAGAAATGTCTGTTTTGAGGAAAGGGCTTTGAATTCCC1230.13170575008030838No Hit
TAATTGGGGGTCCAATTTAAAAATGGAACTAGACATACATAGAGGCATTAAC1220.1306349716243709No Hit
TACTGCGGGACAGCATTTAATAACTGCATTGCCTATTTTCAACTCAGGTTAC1220.1306349716243709No Hit
CAAACCGGGATTACTCTGCATTATAATGAAGCCAACAGTCATATCTTCTGAT1220.1306349716243709No Hit
GTCACAGGGCAAGAGGCTTACAATTATTCTGGAATTTGTGTTTGCTTATTTG1220.1306349716243709No Hit
ACTCTTGGGGGACTGGCGGCAGGCTCGCCGCGGCGCGGAGTCCCGGCTGCGG1210.12956419316843346No Hit
TGTCAGGGCAAGAATTTCCCGAAATTGAGTCCCTGGCACCTGGAGAATCTGC1200.128493414712496No Hit
TTTTACGGGCCCAGATTGTAGGTAGAAGTGTGTTTGCAAAGGCCAGAGGAGC1190.12742263625655853No Hit
GTTGTTGGGATTGATGGATATTTAGCTTAATTCCATATCTTGGTTATTGTGA1190.12742263625655853No Hit
TCTGCCGGGATGATTAGTACTGAGTGTCAACTTGATTGGATTGAAGGATGCA1190.12742263625655853No Hit
TTCAGTGGGATGTGGGTTATGCATCCATAGATTCAACCGACTACACATCAAA1180.12635185780062105No Hit
GAGCAAGGGCTCGAGGCCACAGTCATGCCCTACCTTCAGGTGTTATCAGAAT1180.12635185780062105No Hit
AGTACGGGATCCTTGGTGGCCCTTAATCCAGAATGCATGCCATGATTACACT1180.12635185780062105No Hit
ACCTGAGGGGATCTCTGCTGGAGGTTGTCAAAAGAACACAGCCAAGCACACA1180.12635185780062105No Hit
CTGTATGGGGTAATTTGTTAAATTTGAAATGAACTATTACCTTGAAATATAG1180.12635185780062105No Hit
AATAAAGGGATTCATATGAGAAACAAAACCCCAAAAGCACAACTTTAAATAA1170.12528107934468358No Hit
TCGAAAGGGGATTGTTGTGTGATTTCTGAAGTTTTAATTTAATGAAATCTTT1170.12528107934468358No Hit
CTCAGCGGGAGATCTCATACACCAGAATTCAGATCATGAATGACTGACAGAA1170.12528107934468358No Hit
GTGTCCGGGACATACACAGCCTTCCAGCTTCTATTATGATGCATTGTACTGT1160.12421030088874611No Hit
TGTTTGGGGTACTTCACCTCTGAGCCTCCGATGCTCTGTGAAATGGAAGGAA1160.12421030088874611No Hit
CAGCAAGGGATGGATGTCTGGGCAATCATAGCACTTGCCATTTAAAAACATG1160.12421030088874611No Hit
ATGTACGGGGAGGTACTGGGAGTTAGGACTTCAACAAACAAGTTTGGGATGG1160.12421030088874611No Hit
GCTTTGGGGATCCTACAACCAGCCAAATGCACTTTTGTGAAAATGGGGTGTG1130.12099796552093373No Hit
TCTTTAGGGCTGATAATTTGAATCCTGCCATGAAATTTCCCAAGTTCACTTT1130.12099796552093373No Hit
TTTCCCGGGAAGTTCTGAAGAGTATGCATTTAAAAGTAAAAGTTTTCCCTTA1130.12099796552093373No Hit
GTTAAGGGCAGGCAACATGAATCTGGGGATTCAGTTTCCAATACATGAAATT1130.12099796552093373No Hit
GGTAAGGGGAAGAAATAACTGAAATATGATGGTATTTACATTAATTTTTGAA1130.12099796552093373No Hit
CATTGAGGGCTTGGTCTGCAGTCTGGAAGCTTGTCTGCTCTATAGAAATGAA1130.12099796552093373No Hit
CCAACAGGGGTTACTGTCTGTTTATTGATAACAATCTCTGTGTTATAGATGA1120.11992718706499625No Hit
CGTAAAGGGAAATACAACATGTACACAGGTTTTCATAGCATTATTCATAACA1110.11885640860905879No Hit
GTAGTAGGGGGATGGTGACTTTTATTTCCAAGCACAGCATAATCCACAATAA1110.11885640860905879No Hit
ATGCTCGGGAGCCCCCATCGCTGGGTCAATAGTACTTGCCGCAGTACTCTTA1100.11778563015312131No Hit
GTATATGGGAGAAAAACAAATTGTGTTTTCAAAAGGTGCTAACAAAATATCT1100.11778563015312131No Hit
ACAAAAGGGAAAGACGTCAGTTCTAAGTTGGTTGCAGGGTTCTGGGCAAAGG1100.11778563015312131No Hit
GAGTAACGGGGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAGAAA1100.11778563015312131No Hit
CTTATGGGGTCAGGATCATGACCAAATTTCTCCCAACCAAAAACATTTCCTT1100.11778563015312131No Hit
GGAAGCGGGCATCTCTGGAGGAAGGGATGGGAGCACCCTTCCGACCAGTTTC1090.11671485169718386No Hit
AAATAGGGATTTCTGTTTAGTTTTCTCCAAAAATAAAACACATTTCATATTT1090.11671485169718386No Hit
TCAATAGGGGACATGCTTTTTATTCACTGACTAGTATTCACTTTTTTACAAC1090.11671485169718386No Hit
ACAATTGGGGAGGAGAATTCATCTTCCATCTTTCCTTATGGAGAGACCTTTC1090.11671485169718386No Hit
TTACATGGGGTCTGCACCAGCTAAGTACATGCCTTACATGCATTGTTAATTC1090.11671485169718386No Hit
AGCAGTGGGATTTTCATGAAGTTTTTAAGAAATATTTAATAGAAGGTGCTGT1090.11671485169718386No Hit
GTCATAGGGATTTTAACAGAGATCAGCACTTGGCAACTTGACATGTTGGGCA1080.1156440732412464No Hit
TGTAATGGGATGAGTTCCTGAGACATGCTCTTTTGGGGGCTGGGGCTTTAGG1080.1156440732412464No Hit
ACTTGAGGGGGGGTCAGAATATCACTAATTTTGATTATTATTACAAAGGATT1080.1156440732412464No Hit
ATATATGGGAAAGTTGAACAATTGCTAGCTGATATTATGTGGAAGTTACAGG1080.1156440732412464No Hit
CAAGATGGGATGTATTTTTAGCAAGATAACTTGGGTAATCTTCTAAAAAAGA1070.11457329478530892No Hit
AAAGGGGGACATATTTTGAAACGGCCCTGCAAAGCTGTCCTTTGCGGGAGAA1070.11457329478530892No Hit
TCAACTGGGCTCTTATCACTAAGAAAGAAAGGAAAGAAGGAGATGAATAGAA1070.11457329478530892No Hit
ACTTTGGGGCCCAGAGCCAAGGACTTGTACCCTGTGTAATGTTTGTGCTGAA1070.11457329478530892No Hit
TATCGAAGGGATGACAAAATTATTTATGATAATTGGATGGCTGCAATTGAGG1070.11457329478530892No Hit
AGCATCGGGGGTGTCTTTCGGCAACTTACACACATTGAAAATGAGCTATTGT1060.11350251632937144No Hit
GGTCTAGGGAGAACAAAGGATTGACATCTTTGACAATAATTAGACTGGCATA1050.11243173787343398No Hit
GTTTCTGGGGTTTGATGAAAAGAGATATTTTGCTTAATTTACTTGAATTTAT1050.11243173787343398No Hit
GCATAGGGGACCATTTTAAAACTATAGCTGGATCTAATGACAAATAAATTAT1050.11243173787343398No Hit
GTTGCCGGGGCAGACCATCTTATTGTTATCCTGATTGGATTTCCAGAGCAAT1050.11243173787343398No Hit
AATTGTGGGGAGCTTTTCTGTACAGCAAAATAAACTATCAACAGATTAAACA1050.11243173787343398No Hit
GATTTAGGGAAAGTGGAAACTGATAGCTTCTCCAGATCATACTTGCTCATTC1040.11136095941749653No Hit
TTACCTGGGGCTTTTCGATCCGCCATCTGCGGTGGAGCCGCCACCAAAATGC1040.11136095941749653No Hit
ACTAATTGAGGGTCTATTTGATTCAAATGGTATGTTTTTTCACATTTCTCAT1040.11136095941749653No Hit
TGTTATGGGAGTTTATGTAAGATGTGTGCTATTTCTTCCTTAAGCATTTGGA1040.11136095941749653No Hit
TAAACCGGGTGCTTAATTCAAACTAAAGGGCAAAGGAGTATGGATGGATATC1040.11136095941749653No Hit
ACAGATGGGGAAGCCCAAATTAAAAGTCACAGTTGCGGCCAGGTGTGGTGGC1040.11136095941749653No Hit
TGGTCCGGGAGTAGAACAGAGCGAAGGGTGGCATTTCATTCTTGGTTCTGTT1030.11029018096155906No Hit
GCTTCTGGGAGAAGATATTATGTTATGCTCAGTAAATAATTAAGAGATGGCA1030.11029018096155906No Hit
GCTGCTGGGAACGTGGCACTGGTAGGTTGGGACACCAGGGAAGAAGATCAAC1030.11029018096155906No Hit
AACTGTTTGGGAGATGTGTCCATATATGTCCATATTTACATTTTGATAGCCA1030.11029018096155906No Hit
GACGAAGGGATGAAAACAGCACTGGGAAAATCCACCCCCAATGGGTCTATCC1020.1092194025056216No Hit
GTTTTGGGCACCATAGCAAGAAGGAAATTATTGATTTATGTCCCCAACATCA1020.1092194025056216No Hit
AGTGAAGGGGATGGGAAAGATAAATCAGAACTTAGCTTTCTATGTTAGTCTT1020.1092194025056216No Hit
ACGCGGGCTACCTCAAAGGGACACTTAGTATGCCTAAAATTTATTCACTTAG1020.1092194025056216No Hit
TGAATAGGGACATGCAGTCTCAGAGACTATTTAGACAAAGTTCAAGTTAGGA1020.1092194025056216No Hit
GCTGCTGGGGGAACCAGGTCCCACCTCCCCACAAGGCAAATCTAGACCCATT1020.1092194025056216No Hit
GTCTACGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1020.1092194025056216No Hit
GGATTAGGGCCGCCTCGGCCTCCCAAAGTGCTGGGATTACAGGCGTGAGCCA1010.10814862404968412No Hit
ATTTATGGGCGACGCTCTTCCGATCTAAAGTAGGGAAAGTGTGGAAAGGAAG1010.10814862404968412No Hit
CCACTTTGGTGAGCAGGGGAGCATAAAATTCATGGGATAGGGACTTTGTTTA1010.10814862404968412No Hit
AAGTTTGGGAGGTACCGTTTGTGTAACATTACTCATTTCTGTATATTCCTTT1010.10814862404968412No Hit
AATGCTGGGGACAGATAATTAGTTTATACAGTTTAAATTACCATACCTATCT1000.10707784559374665No Hit
ATCACAGGGAAGTTCAAGTGTTCTGTTTGGTTAACAACCTTGTGTTGTATAT990.10600706713780918No Hit
AATTCTGGGATTTTCTGACATGTTCATATACATTAATTTTTTAAAAAATTCA990.10600706713780918No Hit
ATCAAGCGGGAAGTACCACTGTAAATAATCTTCAGTAGTGGTAAATCACATT990.10600706713780918No Hit
GCAACCGGGATAGGGAGGTGGTGACATTCTGGATACTTCCACCATGGGGCGT990.10600706713780918No Hit
GAACCAGGGGAGAGGATAAATTCACCCTCCTCAAATAGTTGTAAATAGTCCA980.10493628868187173No Hit
GCTTAGGGAGACTTAAAACCATTTACTCCCAAATTTTTGAGTATGCCCTCAC980.10493628868187173No Hit
TATTATGGGCACATATGACTTGTGAATAATTTACATGGGGTATCTATACCTT980.10493628868187173No Hit
GAATGAGGGATCTTCTAAACTGGAATCATTACTTAATTTTAACTAACCAACA980.10493628868187173No Hit
CAGCAAGGGTAGGACTAAAACTAAAAATTAGTGATCGAACAAAATGTGTATG980.10493628868187173No Hit
ATCTAAGGGATAATCACCAAGTTTAAATAATCAGTGTGGCATTTTACAAATG980.10493628868187173No Hit
TTTCTAGGGATCCTTGGATGTACAAAAAATTCAGAAAATGATCTCTGTAGAT980.10493628868187173No Hit
CAGTGCGGGAATTGTATTGGTTTTTAAAAATAATTAAAATGGCAACACTTTG980.10493628868187173No Hit
GTGCCGGGGTATTTTACAAAGAAATTTGAACAAATTTAATTGAATCTTTTGT970.10386551022593425No Hit
CAACGGGGGATAAATTGTCGGTACATTCAGCTCTTCTAGGTGAGCAATACTG970.10386551022593425No Hit
CTGATCGGGATCAATGTCAATATCATATAGTACAATATGATATTATAGTTGC960.10279473176999679No Hit
TGCGGTGGGGTCTGAGAATACGGTGGCGTTTTCCATTGCTAAAAAGAAATGC960.10279473176999679No Hit
TGCATCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.10279473176999679No Hit
GCCAAGGGGAATTTATACCAGTGGTAAAATGGTACCACTTAGTCTGATACTT960.10279473176999679No Hit
ACTTGTGGGAGGGACAGAACTCAATTTATATTATTAATAGCTTGCACTTAAA950.10172395331405931No Hit
ATGTGCGGGATTGTCTCTAGAGGCTAGTCTTTTGAAAACATACTTTTACCCT950.10172395331405931No Hit
GTCCCAGGGAATAAGACAAATGCAGTCTACATTTCAAAATTGGTCATTTTTG950.10172395331405931No Hit
CCATACGGGAGGGATTTCGTGTGTACACTCATTCCATCTTCTCATGTGGCTC950.10172395331405931No Hit
GAGTACAGGGGAGTTTTGAGAGTGTTCTTCCATGTTTTAGAAGCATCTTCAG940.10065317485812185No Hit
GCTAAAGGGGGTTAATAGGTGCACTAATTAATTAATAGATGTATTAATTTTT940.10065317485812185No Hit
GACGCCGGAAGGCTATTTTGAACTTCTGAATAGAATTACACTTACAATAAAA940.10065317485812185No Hit
CCAAAAGGGACTGTTTAGTGGGTAAAGAGTTTCAGTTTTTCAAGGGAAAGAT940.10065317485812185No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)