FastQCFastQC Report
Sat 31 Dec 2022
SRR5849440.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849440.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125978
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT16891.3407102827477815Illumina Paired End PCR Primer 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC15631.2406928193811617No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2670.21194176761021766No Hit
GCTTTTGACCCAAAGGTCCTGACGGTTGCGTCCGGGGGAGGGGATGGAAGGG2570.20400387369223197No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC2440.1936846115988506No Hit
CAGCGCGGGTATAAAGAGAAACAATATGTAAATGCCCATGATTAAAATTGCT2200.17463366619568496No Hit
GGTGCAGGGGATCTGTCACCGGGGTATATGGGGACACTGCCAGTCACCAAGG2140.16987092984489355No Hit
GCAAAAGGGCTTCCGATCTACTTCTGGGCACCTGTGCCAGTAATCCCCCAAC2100.16669577227769927No Hit
CATCATGGGAACTGGGTTTTAAAAATCTGGTTCTAACAGTAGTTTATGTCTT2060.163520614710505No Hit
CGTGGCGGGATTACAAAACTGAATGCAAATGTCCTTTGTGGTGCTTGTTCCT2030.1611392465351093No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT2000.1587578783597136No Hit
GACTGTGGGACTCTGAGGTCCAAATACTGTCTGTCAATGTGTAACCTGATGT1990.15796408896791503No Hit
ACACAGGGGTGATCAGAAGGATGGAAGTGCCATTGAGTGAAACTAGAAAGCT1970.1563765101843179No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA1950.15478893140072075No Hit
AACAAGGGATGTTACTAAGAGACAGTCTTTAAAGGTAATATCAAATTTCTTT1930.15320135261712362No Hit
AAGCCTGGGAACTCCTGAACGGCGCAAGGGCAGTTTAGCTGATGTTGTTGAC1920.15240756322532506No Hit
GGAACGCTGGGGAATGAATGAATGAACGAATGAATGTACTATGAGCTGATAC1920.15240756322532506No Hit
TAGCCGGGATTGTGTGCCAAGTAATCTGTGCTAAATAAAAATAGAGTATTTG1910.1516137738335265No Hit
AAATTTGGGCTTGCTCAGACATTAGCCTTTGTACTATTAAGAACTTTAGCTG1880.14923240565813078No Hit
CTTAGCGGGATATACATTTTTACTCTTGTTGCAAAAATAAACTGTTTCTTGC1880.14923240565813078No Hit
ATCAGGGGAACAGAAAGAAATGAAGTGACTCAGCTTCTGGCTTCTCTGCTAC1870.1484386162663322No Hit
GTGGCCGGGATCAAAATGTGCTTTGAAGGAAACAGGCCTAATCCTAAGGTAC1860.14764482687453365No Hit
TACGTGGGGGAGTCGCCGCGGGACCGGGGCCGCTGGGGTCTGGACGGGGGTC1860.14764482687453365No Hit
TCTAATGGGAGACTTTGAATTAAGGGAATGTTGTAGCTGGTTTGATCTTCTA1850.14685103748273506No Hit
CATCACGGGGAATATAAAATACATACCTATACAATTTGAGAAGCCACTTGAG1850.14685103748273506No Hit
TAGATAGGGCATTGTGGGAGTTGCTGATACTGGGGAAATGATGGCAGATCTG1840.1460572480909365No Hit
GCTGCAGGGATATCCAGTGTTAAGAGTTTTAGGAGAATGTTGCTAACATTTG1820.14446966930733937No Hit
AATCCAGGGATATTGATAAATGGGTTGAAGTCTATTTTTCCATTATCATCTT1790.14208830113194368No Hit
TTCAATGGGAATTCAGTTTTTCATAGCAAGTGAATAAATTAGCATTCTGGCA1790.14208830113194368No Hit
TCATGTGGGCCAATACTCATTTAATTAAGATTTAATCATTTGCTAATGGAAA1780.1412945117401451No Hit
CGTGCTGGGTATGTATGTATAGCTTGAGTATTGAGAGATCTCTTAGAAATGC1780.1412945117401451No Hit
AAACCAGGGACAAAACATTGCTGAAAGAAATCACAGGAGAACTAAATAAATA1770.14050072234834654No Hit
ATGCGTGGGGGATAGTTAAGGGAGGTTGTCTGAACAGGGATTATAATTAGTT1770.14050072234834654No Hit
TTAGCAAGGACTTTTCAGTTAGGTAACTGTGTGATCCAGTTCTTCCAGCTGC1730.13732556478115227No Hit
TGGATTGGGAGTGGACTATTATTTTGTGCATCAAATTCTTAAAAACGAAAAT1730.13732556478115227No Hit
ACGTTTGGTATGAAATAATGAAATTGAAATGAGATGAGATGAGATGAAATAA1700.13494419660575657No Hit
TAGTGCGGGATTCATAGTAGGAAATTTAGAAAATAGAAAATGATAAAAACAA1690.134150407213958No Hit
GCTAAAGGGACAGGATATGAATTGAATTTAAATATTTCTAGGTTATGGGAAA1690.134150407213958No Hit
GCAATGGGACAGACTGGATCCTCAAGGACTACTTTATGAGCTTTTACTTTCC1680.1333566178221594No Hit
TCTGCGGGGGTTGGAAGACTATTATTAATATTAATTGGGGACAGGGCATTGC1680.1333566178221594No Hit
GTAGTTGGGCACTGCGCTGGTCCCTGCTCAGTTTATGGAATCATTTCACTGA1670.13256282843036085No Hit
GTAAGAGGGATATAAATTTGAGGAAGGCACGATTTCACTCATAACAATCTTA1670.13256282843036085No Hit
GATTGAGGGCATTTCAATAGACACAGAAAAAGCTTTTGATAAAATCCAGTAT1660.1317690390385623No Hit
AGTTCCGGGGGTAGCCAAGGTTGAGAACCAGTACCGTACGTACAAAAAAGTT1660.1317690390385623No Hit
AGATAAGGGGTCTCTTAGGTAACTTCCTGTAAACGATTTGGAAATAGGATGT1660.1317690390385623No Hit
AAGACTGGGGTGGAGGAAAGATTATGAAAGGAACAGGCTCTTTAAAAAAGAA1650.13097524964676371No Hit
AGTTAAGGGAACTGAAATAATTCTTAAAGTGCTGATTTAAAACATTAATCTT1640.13018146025496516No Hit
AGTAAAGGGTCTGCAAGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAG1640.13018146025496516No Hit
CGTAGCGGGATACTTTGCTAATTTGATCATAGCACCTAGATGGATCCTTATG1630.1293876708631666No Hit
GCTGTGGGGATGGAATTCATTGGAATGTGCTTAAAATGCTAAAGTGTATGTC1620.12859388147136802No Hit
ATCGCTGGGATCAATTAGAGGCTCATAGACTATATTAGGGACAATCTCAGGG1620.12859388147136802No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1620.12859388147136802No Hit
ATAATAGGGGTGACCACTTTTTCAATACTGTGAGATTATGGTTATGTGAAGA1610.12780009207956944No Hit
GACAGGGAATTAACTTTCATATTAGCAGATTAAACTAGCAGAGCAGGTTCAA1600.12700630268777088No Hit
CTAGGCGGGATTTTTTACCTGTTCCTGTCAACTTCTGTTTTCCAACTCACCT1590.1262125132959723No Hit
CAAGCCGGGGATCTTTGGTGGGGTCTGCATACGGGATCTCTTCCTAGATTAT1590.1262125132959723No Hit
GTATTTGGGATTTTTCTACATGCTCTTTAGGGTTTTAAATGTCAACCTACTG1570.12462493451237516No Hit
TCAGCAGGGCTTGAGGAGTGTTAGCAAAATTTAAATTTTGACCCTGGCCATT1570.12462493451237516No Hit
ATTCGCGGGCTTTCTCTCTTTTGTTTTGTTTGTTTGTTTGTTAAAGTTCATT1570.12462493451237516No Hit
TGTCTAGGGAATTGACAAAATTATCAGGAGATCTCAATCAACAATCAATGTA1570.12462493451237516No Hit
TCAAACGGGAACCTGGGTTAGGAAAGATGGATAGAGATAAAAGAGAAAAGAA1560.12383114512057661No Hit
GTGTTCGGGATATTGGACTTGTTTTTTCCTGCTGAGGAAATCTAATTTATGA1560.12383114512057661No Hit
AGGTAAGGGAGTTCTTACACCTTGTTGGAGAAACTACAAAAGAAAGTTGTCC1560.12383114512057661No Hit
TTTGTCGGGAAGGTATGATCTCCAGAAGTATCTCTCAACTGCTTAAATTAGG1550.12303735572877805No Hit
CTAACAGGGGCAAATCCCTAGTTTGTATCCTGTGGATTTAATTTCTAACAAT1550.12303735572877805No Hit
CAATATGGGTCTGAAGCGGGCTGTAACAACTAGTTTTAATGAGTAAAAAGTC1540.12224356633697947No Hit
TCGACTGGGTCCCCATCAAAAGACATATATTAGTGGATTGTATTAAAAAATA1540.12224356633697947No Hit
ATTGCTGGGAGATCAAAGACTTAAGTCTAAGACCTGAAACATAAAGATTCTA1530.1214497769451809No Hit
TAAATAGGGATCCCATTTGTCAATTTTGGCTTTTGTTGCCATTGCTTTTGAT1530.1214497769451809No Hit
AATTATGGGATTGACTCTGAGCAACACAAATGTGGTCTGTTTCAAAGTGAGG1520.12065598755338235No Hit
CATCAGGGGGACACTGACGTTTTGTCTCTTGTTCCCCAGCCCCCAGCCCATG1510.11986219816158376No Hit
ATCTGCGGGCCCAGCCAGCATTTCAGTCCCTCTCCCCGACTCTTAATCATGA1500.11906840876978521No Hit
TATTAAGGGATTGCTTTCCATAAAATAAAGTGTCCTTTTTGTCTCGTATAGC1500.11906840876978521No Hit
AGCTCAGGGATTGAAGGATAATTTCTTAGGGTACAGAATTCTTGGTTGGTGT1500.11906840876978521No Hit
ATTTAGGGGACATGGGATAGTTTATGTGATTCCTCTATAAGCCAGGACCTAA1500.11906840876978521No Hit
TAGCTCGGGGAAACGGAAGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCA1490.11827461937798664No Hit
TAGTGCGGGACGTGGACAGTTCTTATGCCTATCATGTCAAGACCTAGCTCCT1490.11827461937798664No Hit
AATGCTGGGCTACTGAGTGGCAGAGCCAGGGTCTGGGCCCAGCCAGTGTGGC1490.11827461937798664No Hit
TCTGGAGCGGGAAGTGATTTCAGTCAAAACTGGAAAACAACTAAGATGTAGT1480.11748082998618806No Hit
GACACTGGGATGTTTGGTTGATGCGAGCAGCTGCACTGCTCATGCAGTTAGC1480.11748082998618806No Hit
TAGCTTGGGGAGCTGTCCTATTAAAAGACAGAGACTGTCAGATTAGATTTTT1470.1166870405943895No Hit
GCTTTTGGGAACTAAGCTAATGATTCTTCCTTTTCTACTTTAGGAGAAAACT1460.11589325120259092No Hit
TTAACTGGGAACGTGTGACTGAACTAGAATTTCTTTTGTTGGAGGGGGATTG1460.11589325120259092No Hit
ATTTTGGGGATATTTTGTCCCTGAGACTGTGAAGTGAGTTGAATCTGTATAA1450.11509946181079236No Hit
GCTTGAGGGGACTCAATTCAGTATCACCTCGTGGTTTTATCTCATAGTAATG1450.11509946181079236No Hit
GCCGACGGGAGTTGAAAAACTGAAGACGCTGCCTAAAATACAAGCTGAGTTA1450.11509946181079236No Hit
TAGCCAGGAATGAAATTCCTTTCAAAACCCCAGCAAGATTTTTTTGTAGATA1440.1143056724189938No Hit
CAGTATGGGAGGAGTTCAAGATGCAGAGATGGCAAGATTCCATTTCTACAAA1440.1143056724189938No Hit
AGACTGGGGGTCTGATACCGTTGTTATAACAAAACAAATTTTTTTACTATAG1440.1143056724189938No Hit
AAAACGGGGCCACTATGAAGAACAGTTCGGAGGTTCCTCAAAAGGCTGAAAA1430.11351188302719521No Hit
CTATGCGGGTATAGGTCAAGGACTAGTTTCCCAGTGAATTGATTTAAAGAAC1430.11351188302719521No Hit
ATCTACGGGACTCAAAATAATTTGATACTAAAAACTGAATTCCTGTAGCACA1430.11351188302719521No Hit
AACGTAGGGACTGGTACCCCAGGAGGATGCCTTGTAACTGCTCTTTGGTCAT1430.11351188302719521No Hit
ATACACGGGGGCAAGAGGATCATTTGAGCCCAGGAGTTTGAGACCAGCCTGG1430.11351188302719521No Hit
ATTTTGGGGCTGACCACGTCTATCTCTGGGGGTCGCACGTTGCGGGCAGAGC1420.11271809363539666No Hit
GCAATCGGGGATGTTACACTAGAATCAGGTTGGAATTTGGTGTCTTATTGCT1420.11271809363539666No Hit
AAAAGCGGGGGTATTTTTTCATTAATAAAACCATCTTTGAAACATTCAACAT1410.1119243042435981No Hit
CTACCTGGGGAAATGAAACTTAAGCACTCATTTGCCTTTGTCAGTGTACGCA1410.1119243042435981No Hit
AAAAAGGGAGGGCTTACAGAAATGAATAAGTGGGTGGCACTAAATTATGGGA1410.1119243042435981No Hit
AAAGTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1400.11113051485179952No Hit
TTGCATGGGGATATAATAAATACTATAAAATTGTAAAATAGGCTAATAAATA1400.11113051485179952No Hit
CAAGCCGGGATAGAAGATAGTTTTGTTGAAAATGATTGTAGGTTTATACTTT1390.11033672546000095No Hit
TCTAGTGGGAGTCTTTTAAAATTGAGAGTCCTAGGCCTACAATTGTCTTTCT1390.11033672546000095No Hit
GTCAACGGGAGCCTGATTGCGGACATATTTTAAGAAAGGAGGGAGGGAAGAA1390.11033672546000095No Hit
TGAAAAGGGATGGGTCAGTTGTCAAGAATGTTCTTTGGAACACATTGTATAA1380.1095429360682024No Hit
AATGTGGGGGGGGGAGTGGAGAGGGGTTACATAACAGTATTGTAGAAAACAA1380.1095429360682024No Hit
GCTGCGGGATATACAATCAATGCTATTTATTGTACCTCTGGGCCTACTCTTC1380.1095429360682024No Hit
GGGTCAGGGAAGAGGAGCAGTGAAATGTATATAGTTGATGTTCAGTATTTCC1370.10874914667640381No Hit
GCGCTAGGGGTGTGGGTAACAAACTTTGATCGTGTATGAACAATAATATTAC1370.10874914667640381No Hit
ACAGCAGGGAGAGAAGCTTCATCTATGATGAGCCTAGCAAAGATACTCTCTC1370.10874914667640381No Hit
GAATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1360.10795535728460526No Hit
TAGCAGGGGAGAATTTTAGGGACCAGAAGCAAACTGGTAATTCTCTGCATTT1360.10795535728460526No Hit
TATTCCGGGGTATTTTCTTTTTCATGATTATCGGTGACTGGTCAGTGTACTC1350.10716156789280668No Hit
CAAAAGGGGCCGATCTTGGTAGGGGAACAAAAGAAGTAAAAGATCTCTATAC1350.10716156789280668No Hit
TTTGCTGGGAAGATGGAGGAAGGCGGGAACCTAGGAGGCCTGATTAAGATGG1350.10716156789280668No Hit
TATCAAGGGATGTTAGCTAAGGTTTTTTTACAGTTAATTTTGTCTTTCTTCT1340.1063677785010081No Hit
TTACTGGGATCAGAGCAGAAACAAATGAAATTAAAGTGAAAAAAAAGTGCAA1340.1063677785010081No Hit
AACGCAGGGATGCCATATATACAGACTCCAGTGTGGTTATTTTCTCTTCTTA1340.1063677785010081No Hit
ATTGGGGATATCACAGCATAACCCCACCCTTTACATTTTGTGCAGTGATTAT1340.1063677785010081No Hit
GGGCACGGGAGAACTAGAAATCCCATTTGACCCAGCAATCCCATTACTGGAT1330.10557398910920955No Hit
GGATACGGAGTGATGAGAAGTGATTGATCCCTAGTTCCTGGTCATCAGCCTA1330.10557398910920955No Hit
CTAGATGGGTCAGCCAAAAAATTATTTCCCAGCAGAAGAATCTTTTCCTACC1320.10478019971741097No Hit
CTCATAGGGATTCTCAAGGAAACACCATGTTGTCCCACAAAATTAGTCTCAA1320.10478019971741097No Hit
GAATAGGGAATACATAATTCAAAAATTGTGAAAGTTTATTAAAATGTAATGC1320.10478019971741097No Hit
CGTGCTGGGGTTTGTAGAGTGCAGGAATAGTATTATACATATGAAAACACAA1310.10398641032561241No Hit
TTGTGAGGGATGACTAAAGAGTAATCTTGTTTTGTTCTTTTTCATAGATATT1310.10398641032561241No Hit
ATATAAGGGGATCTGTGTTAGGGGTGTGTTAAGGAGGAATTACAACGTACTT1310.10398641032561241No Hit
CTCGCAGGGGTACAAATTATTTCATCACCCAGGTATTAAGCCTAGTACACAT1310.10398641032561241No Hit
TGACATGGGATATCAGATTATAGAATAAAGAACTCTGAGAACTCAGTAATAA1300.10319262093381384No Hit
AAAATGGGGCTGCTGATTTTGCTTGTGGCCCTTAGCATCAGATGGCCTTGAG1300.10319262093381384No Hit
CGGACCGGGCGATCTTTGTAAGGGGTGAACAGTTTCTGAACAGAACACTTTG1300.10319262093381384No Hit
TCGCTTGGGGTGATGATGGTGCGGTCATATTTTATAGGGATAAAACAGAAGA1300.10319262093381384No Hit
GTAGATGGGCATTTCTTTATGAGTGATGATTATTTATTCTAGCATCTCCTCT1300.10319262093381384No Hit
CTAACGGGGTCTTAGGAAGGCCCATAATACTTGTTTTTCTTACATGTGACTA1300.10319262093381384No Hit
GAGGGATACCCAAAGGACTATAAATCATGCTGCTATAAAGACACTTGTACAC1290.10239883154201526No Hit
TTAGTAGGGACAGTGATTTATATGTTACTTTTTAATATTCATTTTTTGCTTT1290.10239883154201526No Hit
CTCATTGGGATCCAGTAGCTAACATTAGATCTAGCTTATTATTTCAGAAATT1290.10239883154201526No Hit
CTTCTAGGGAGGACTATCTGGTGATGAATCTATCCACTAGAAGCTGGTAAGT1290.10239883154201526No Hit
GCGCTTGGGAGTTCCTTAGTTTACATATGTTTGTGCAATTATTTTCTGTACT1290.10239883154201526No Hit
TCTGCCGGGGAGTTTTTCTATGCAGGGAGTCATTCACACATCCAGCAAGGAA1290.10239883154201526No Hit
TTAGTAGGGCTACGCAGGGGGCCATGAGTGGGGGTGCACCGTATTACTTTGC1280.1016050421502167No Hit
TGTAATGGGTCTCTTCTGGACTATATAAATTACATATGTAGTTAATGACAAA1280.1016050421502167No Hit
CAATTGGGATAGCAGAACTAGAACTTGAAAATTGACTCTTTGCTTATTCATG1280.1016050421502167No Hit
ATTGCAGGGTATTGAGGGATTTTTTATTTTCAAATGTTGCAATTTTATATAA1280.1016050421502167No Hit
GTGGTGGGCAGGCCACATACTGGGAGAAAATATTTCCAAACCACATATCCAA1270.10081125275841815No Hit
ACATTTGGGTAGGAAGGAGAAAACTCAGGAGGGAGGAGACCAACTTCAAAGA1270.10081125275841815No Hit
TTGTCTGGGGTTAGAATTTGGGCAACATTTTGATTATAATGACAACTTCATT1270.10081125275841815No Hit
TTCACCGGGATGGGAATGCTCTTTAGAATTCATTTTGTTCAGCCCCTTTGTT1270.10081125275841815No Hit
TCAGCAGGGGCCATCTAGTTCTTTTCTTCACATGACACTTTTAAACAGCCAG1270.10081125275841815No Hit
TAAGCCGGGACTGTTATTCTTTTCTGTACCAATTCCATTTTAACTTCTACAG1270.10081125275841815No Hit
GTATAAGGGATCACAAAAAAGGGAGACTTTAATTTTGGAGGAAATATGGGAT1260.10001746336661957No Hit
GCCAAAGGGATGGTCATTTTAGATATTGATTTCTTCTTAAATATAATGAAAA1260.10001746336661957No Hit
AAAACTGGGACCAAGTTCATGGTGATGTTGACAGTATCTGGCAAGTAATATC1260.10001746336661957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)