FastQCFastQC Report
Sat 31 Dec 2022
SRR5849439.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849439.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124942
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT16491.319812392950329Illumina Paired End PCR Primer 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC15181.214963743176834No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2540.20329432856845578No Hit
GCTTTTGACCCAAAGGTCCTGACGGTTGCGTCCGGGGGAGGGGATGGAAGGG2420.1936898721006547No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC2290.18328504426053688No Hit
TCTGCGGGGGTTGGAAGACTATTATTAATATTAATTGGGGACAGGGCATTGC2230.17848281602663635No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA2130.1704791023034688No Hit
TACGTGGGGGAGTCGCCGCGGGACCGGGGCCGCTGGGGTCTGGACGGGGGTC2100.16807798818651853No Hit
GGTGCAGGGGATCTGTCACCGGGGTATATGGGGACACTGCCAGTCACCAAGG2090.16727761681420178No Hit
AAGACTGGGGTGGAGGAAAGATTATGAAAGGAACAGGCTCTTTAAAAAAGAA2090.16727761681420178No Hit
CAGCGCGGGTATAAAGAGAAACAATATGTAAATGCCCATGATTAAAATTGCT2010.16087464583566777No Hit
GCAATGGGACAGACTGGATCCTCAAGGACTACTTTATGAGCTTTTACTTTCC1990.15927390309103423No Hit
GATTGAGGGCATTTCAATAGACACAGAAAAAGCTTTTGATAAAATCCAGTAT1940.15527204622945046No Hit
ATCAGGGGAACAGAAAGAAATGAAGTGACTCAGCTTCTGGCTTCTCTGCTAC1920.15367130348481695No Hit
TCATGTGGGCCAATACTCATTTAATTAAGATTTAATCATTTGCTAATGGAAA1900.15207056074018344No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT1900.15207056074018344No Hit
GCTGTGGGGATGGAATTCATTGGAATGTGCTTAAAATGCTAAAGTGTATGTC1880.15046981799554993No Hit
GCTGCAGGGATATCCAGTGTTAAGAGTTTTAGGAGAATGTTGCTAACATTTG1870.14966944662323317No Hit
CATCATGGGAACTGGGTTTTAAAAATCTGGTTCTAACAGTAGTTTATGTCTT1850.14806870387859966No Hit
ATGCGTGGGGGATAGTTAAGGGAGGTTGTCTGAACAGGGATTATAATTAGTT1850.14806870387859966No Hit
GACAGGGAATTAACTTTCATATTAGCAGATTAAACTAGCAGAGCAGGTTCAA1830.14646796113396615No Hit
AAATTTGGGCTTGCTCAGACATTAGCCTTTGTACTATTAAGAACTTTAGCTG1830.14646796113396615No Hit
ATTTTGGGGCTGACCACGTCTATCTCTGGGGGTCGCACGTTGCGGGCAGAGC1820.1456675897616494No Hit
CGTGGCGGGATTACAAAACTGAATGCAAATGTCCTTTGTGGTGCTTGTTCCT1810.14486721838933267No Hit
AAGCCTGGGAACTCCTGAACGGCGCAAGGGCAGTTTAGCTGATGTTGTTGAC1800.1440668470170159No Hit
AAACCAGGGACAAAACATTGCTGAAAGAAATCACAGGAGAACTAAATAAATA1790.14326647564469913No Hit
TCAGCAGGGGCCATCTAGTTCTTTTCTTCACATGACACTTTTAAACAGCCAG1790.14326647564469913No Hit
AAAAAGGGAGGGCTTACAGAAATGAATAAGTGGGTGGCACTAAATTATGGGA1780.14246610427238238No Hit
TAGCCGGGATTGTGTGCCAAGTAATCTGTGCTAAATAAAAATAGAGTATTTG1770.14166573290006562No Hit
CATCACGGGGAATATAAAATACATACCTATACAATTTGAGAAGCCACTTGAG1770.14166573290006562No Hit
AGATAAGGGGTCTCTTAGGTAACTTCCTGTAAACGATTTGGAAATAGGATGT1760.1408653615277489No Hit
TTCAATGGGAATTCAGTTTTTCATAGCAAGTGAATAAATTAGCATTCTGGCA1760.1408653615277489No Hit
GCAAAAGGGCTTCCGATCTACTTCTGGGCACCTGTGCCAGTAATCCCCCAAC1760.1408653615277489No Hit
CTCGCAGGGGTACAAATTATTTCATCACCCAGGTATTAAGCCTAGTACACAT1760.1408653615277489No Hit
CGTGCTGGGTATGTATGTATAGCTTGAGTATTGAGAGATCTCTTAGAAATGC1750.1400649901554321No Hit
AATGCTGGGCTACTGAGTGGCAGAGCCAGGGTCTGGGCCCAGCCAGTGTGGC1740.13926461878311536No Hit
TTTGTCGGGAAGGTATGATCTCCAGAAGTATCTCTCAACTGCTTAAATTAGG1730.1384642474107986No Hit
AATCCAGGGATATTGATAAATGGGTTGAAGTCTATTTTTCCATTATCATCTT1720.13766387603848185No Hit
CAATATGGGTCTGAAGCGGGCTGTAACAACTAGTTTTAATGAGTAAAAAGTC1720.13766387603848185No Hit
AACAAGGGATGTTACTAAGAGACAGTCTTTAAAGGTAATATCAAATTTCTTT1720.13766387603848185No Hit
CAAAAGGGGCCGATCTTGGTAGGGGAACAAAAGAAGTAAAAGATCTCTATAC1720.13766387603848185No Hit
GTGGCCGGGATCAAAATGTGCTTTGAAGGAAACAGGCCTAATCCTAAGGTAC1680.13446239054921483No Hit
TAGTGCGGGACGTGGACAGTTCTTATGCCTATCATGTCAAGACCTAGCTCCT1680.13446239054921483No Hit
TAGATAGGGCATTGTGGGAGTTGCTGATACTGGGGAAATGATGGCAGATCTG1670.13366201917689807No Hit
GTATTTGGGATTTTTCTACATGCTCTTTAGGGTTTTAAATGTCAACCTACTG1650.13206127643226456No Hit
AGCTCAGGGATTGAAGGATAATTTCTTAGGGTACAGAATTCTTGGTTGGTGT1650.13206127643226456No Hit
TAAATAGGGATCCCATTTGTCAATTTTGGCTTTTGTTGCCATTGCTTTTGAT1650.13206127643226456No Hit
AGCCGAGGGGGCTGTTGGAGTTGCTTGTGGCATGTGCCTGGGCTGGAGCCTT1640.1312609050599478No Hit
TAGCCAGGAATGAAATTCCTTTCAAAACCCCAGCAAGATTTTTTTGTAGATA1630.13046053368763105No Hit
ACAGCAGGGAGAGAAGCTTCATCTATGATGAGCCTAGCAAAGATACTCTCTC1630.13046053368763105No Hit
AGTTAAGGGAACTGAAATAATTCTTAAAGTGCTGATTTAAAACATTAATCTT1620.1296601623153143No Hit
GCTAAAGGGACAGGATATGAATTGAATTTAAATATTTCTAGGTTATGGGAAA1620.1296601623153143No Hit
TCAGCAGGGCTTGAGGAGTGTTAGCAAAATTTAAATTTTGACCCTGGCCATT1610.12885979094299757No Hit
ATCGCTGGGATCAATTAGAGGCTCATAGACTATATTAGGGACAATCTCAGGG1600.1280594195706808No Hit
AATTATGGGATTGACTCTGAGCAACACAAATGTGGTCTGTTTCAAAGTGAGG1590.12725904819836403No Hit
ATCAGGGGATAATAGAGAAGGGAGTTTTATGGAAGTTTCTTTGAAGATTTTT1590.12725904819836403No Hit
CATCAGGGGGACACTGACGTTTTGTCTCTTGTTCCCCAGCCCCCAGCCCATG1590.12725904819836403No Hit
TAGTGCGGGATTCATAGTAGGAAATTTAGAAAATAGAAAATGATAAAAACAA1580.12645867682604728No Hit
GCCGACGGGAGTTGAAAAACTGAAGACGCTGCCTAAAATACAAGCTGAGTTA1580.12645867682604728No Hit
TTACTGGGATCAGAGCAGAAACAAATGAAATTAAAGTGAAAAAAAAGTGCAA1580.12645867682604728No Hit
CTTAGCGGGATATACATTTTTACTCTTGTTGCAAAAATAAACTGTTTCTTGC1580.12645867682604728No Hit
AAAATGGGGCTGCTGATTTTGCTTGTGGCCCTTAGCATCAGATGGCCTTGAG1570.12565830545373052No Hit
ACACAGGGGTGATCAGAAGGATGGAAGTGCCATTGAGTGAAACTAGAAAGCT1570.12565830545373052No Hit
TAGCTTGGGGAGCTGTCCTATTAAAAGACAGAGACTGTCAGATTAGATTTTT1570.12565830545373052No Hit
TCTAGTGGGAGTCTTTTAAAATTGAGAGTCCTAGGCCTACAATTGTCTTTCT1570.12565830545373052No Hit
ATTTAGGGGACATGGGATAGTTTATGTGATTCCTCTATAAGCCAGGACCTAA1570.12565830545373052No Hit
GTAGTTGGGCACTGCGCTGGTCCCTGCTCAGTTTATGGAATCATTTCACTGA1560.12485793408141378No Hit
TGGATTGGGAGTGGACTATTATTTTGTGCATCAAATTCTTAAAAACGAAAAT1560.12485793408141378No Hit
GGAACGCTGGGGAATGAATGAATGAACGAATGAATGTACTATGAGCTGATAC1560.12485793408141378No Hit
TCTAATGGGAGACTTTGAATTAAGGGAATGTTGTAGCTGGTTTGATCTTCTA1550.12405756270909701No Hit
GACTGTGGGACTCTGAGGTCCAAATACTGTCTGTCAATGTGTAACCTGATGT1540.12325719133678026No Hit
TAGCTCGGGGAAACGGAAGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCA1520.12165644859214676No Hit
CTCTTTGGGGTTGGTGGGTGCATTTTAATTTTTTCATTCCCTGGACTATGGG1520.12165644859214676No Hit
GTAAGAGGGATATAAATTTGAGGAAGGCACGATTTCACTCATAACAATCTTA1520.12165644859214676No Hit
TTAGTAGGGACAGTGATTTATATGTTACTTTTTAATATTCATTTTTTGCTTT1510.12085607721983001No Hit
GGATACGGAGTGATGAGAAGTGATTGATCCCTAGTTCCTGGTCATCAGCCTA1510.12085607721983001No Hit
GTAGATGGGCATTTCTTTATGAGTGATGATTATTTATTCTAGCATCTCCTCT1500.12005570584751324No Hit
GTGAATGGGATAAAACAAGGACAAATGTACACAGTGTGTAAAAATGAATAAT1500.12005570584751324No Hit
AGTGCTGGGATTTTCTAATTGCCATCAAATGTCTATGACCCTCAGAAAGCAT1480.11845496310287974No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1480.11845496310287974No Hit
TGTCTAGGGAATTGACAAAATTATCAGGAGATCTCAATCAACAATCAATGTA1480.11845496310287974No Hit
ATTTTGGGGATATTTTGTCCCTGAGACTGTGAAGTGAGTTGAATCTGTATAA1470.11765459173056299No Hit
ACGTTTGGTATGAAATAATGAAATTGAAATGAGATGAGATGAGATGAAATAA1470.11765459173056299No Hit
AAAGTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1470.11765459173056299No Hit
TTGCATGGGGATATAATAAATACTATAAAATTGTAAAATAGGCTAATAAATA1470.11765459173056299No Hit
TAGCAGGGGAGAATTTTAGGGACCAGAAGCAAACTGGTAATTCTCTGCATTT1470.11765459173056299No Hit
TGTCACGGGCAGCTGCCCTCTCCCCCAACTATGACCATGTGGTCCTGGGCGG1460.11685422035824623No Hit
AACGTAGGGACTGGTACCCCAGGAGGATGCCTTGTAACTGCTCTTTGGTCAT1460.11685422035824623No Hit
ACCAGCCTGGTCTGAGGCGTGCTCTGTCCTTAGAGAAGGCGCGGTGGCCGGG1460.11685422035824623No Hit
TTCTTAGGGAGGCAATTTCAATATAATGTAGAAGTAGTAGTGTGCCCTGAAA1450.11605384898592946No Hit
AACGCAGGGATGCCATATATACAGACTCCAGTGTGGTTATTTTCTCTTCTTA1450.11605384898592946No Hit
AGTAAAGGGTCTGCAAGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAG1450.11605384898592946No Hit
GTGTTCGGGATATTGGACTTGTTTTTTCCTGCTGAGGAAATCTAATTTATGA1450.11605384898592946No Hit
TCGCTTGGGGTGATGATGGTGCGGTCATATTTTATAGGGATAAAACAGAAGA1440.11525347761361271No Hit
ATACACGGGGGCAAGAGGATCATTTGAGCCCAGGAGTTTGAGACCAGCCTGG1440.11525347761361271No Hit
CTAGATGGGTCAGCCAAAAAATTATTTCCCAGCAGAAGAATCTTTTCCTACC1430.11445310624129597No Hit
GCTTGAGGGGACTCAATTCAGTATCACCTCGTGGTTTTATCTCATAGTAATG1430.11445310624129597No Hit
TCAAACGGGAACCTGGGTTAGGAAAGATGGATAGAGATAAAAGAGAAAAGAA1430.11445310624129597No Hit
ATTCGCGGGCTTTCTCTCTTTTGTTTTGTTTGTTTGTTTGTTAAAGTTCATT1430.11445310624129597No Hit
TATTCCGGGGTATTTTCTTTTTCATGATTATCGGTGACTGGTCAGTGTACTC1420.11365273486897921No Hit
TCTGGAGCGGGAAGTGATTTCAGTCAAAACTGGAAAACAACTAAGATGTAGT1420.11365273486897921No Hit
CTAACAGGGGCAAATCCCTAGTTTGTATCCTGTGGATTTAATTTCTAACAAT1420.11365273486897921No Hit
TTGTCTGGGGTTAGAATTTGGGCAACATTTTGATTATAATGACAACTTCATT1420.11365273486897921No Hit
AGTTCCGGGGGTAGCCAAGGTTGAGAACCAGTACCGTACGTACAAAAAAGTT1410.11285236349666246No Hit
TTCACCGGGATGGGAATGCTCTTTAGAATTCATTTTGTTCAGCCCCTTTGTT1410.11285236349666246No Hit
CAATGCGGGCGATCTAAGTACGGGACCTGTTCAAATATACATGTAACTATCT1400.11205199212434569No Hit
ACATTTGGGTAGGAAGGAGAAAACTCAGGAGGGAGGAGACCAACTTCAAAGA1400.11205199212434569No Hit
AAAAGCGGGGGTATTTTTTCATTAATAAAACCATCTTTGAAACATTCAACAT1390.11125162075202893No Hit
CGTAGCGGGATACTTTGCTAATTTGATCATAGCACCTAGATGGATCCTTATG1390.11125162075202893No Hit
CTCATTGGGATCCAGTAGCTAACATTAGATCTAGCTTATTATTTCAGAAATT1390.11125162075202893No Hit
TTTGCTGGGAAGATGGAGGAAGGCGGGAACCTAGGAGGCCTGATTAAGATGG1390.11125162075202893No Hit
ATTGGGGATATCACAGCATAACCCCACCCTTTACATTTTGTGCAGTGATTAT1390.11125162075202893No Hit
TCTGCCGGGGAGTTTTTCTATGCAGGGAGTCATTCACACATCCAGCAAGGAA1390.11125162075202893No Hit
CGTGCTGGGGTTTGTAGAGTGCAGGAATAGTATTATACATATGAAAACACAA1380.1104512493797122No Hit
CTAGGCGGGATTTTTTACCTGTTCCTGTCAACTTCTGTTTTCCAACTCACCT1380.1104512493797122No Hit
GTATTTGGGGACTGAGGCCCTGCATTCCCCATCCCACCCCCGGCTGGACTGC1370.10965087800739544No Hit
GTTTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1370.10965087800739544No Hit
AGGTAAGGGAGTTCTTACACCTTGTTGGAGAAACTACAAAAGAAAGTTGTCC1370.10965087800739544No Hit
GACACTGGGATGTTTGGTTGATGCGAGCAGCTGCACTGCTCATGCAGTTAGC1370.10965087800739544No Hit
TCGACTGGGTCCCCATCAAAAGACATATATTAGTGGATTGTATTAAAAAATA1370.10965087800739544No Hit
TCACCAAGGGGTGCAGCCTTGTGGTTTATGCTTAAATGTACATTTTCCTGCT1370.10965087800739544No Hit
ATTGCTGGGAGATCAAAGACTTAAGTCTAAGACCTGAAACATAAAGATTCTA1360.10885050663507868No Hit
GGGTCAGGGAAGAGGAGCAGTGAAATGTATATAGTTGATGTTCAGTATTTCC1360.10885050663507868No Hit
TCATGAGGGCAATGATTACACAAAACCTTTTAAAATTTTAAAAAATTATTTC1360.10885050663507868No Hit
ATCTACGGGACTCAAAATAATTTGATACTAAAAACTGAATTCCTGTAGCACA1360.10885050663507868No Hit
CTACCTGGGGAAATGAAACTTAAGCACTCATTTGCCTTTGTCAGTGTACGCA1360.10885050663507868No Hit
TAATCTGGGGTAGCACAGCACACAAAGGAATGGTTAGAAATGATCAATACCC1360.10885050663507868No Hit
ATGCGAGGGATGTGTTGGGGACTGTGCCAAGTTCTGGGGACACAGACGTGAA1360.10885050663507868No Hit
ATAATAGGGGTGACCACTTTTTCAATACTGTGAGATTATGGTTATGTGAAGA1360.10885050663507868No Hit
GGGCACGGGAGAACTAGAAATCCCATTTGACCCAGCAATCCCATTACTGGAT1360.10885050663507868No Hit
TGAAAAGGGATGGGTCAGTTGTCAAGAATGTTCTTTGGAACACATTGTATAA1350.10805013526276191No Hit
AATGTGGGGGGGGGAGTGGAGAGGGGTTACATAACAGTATTGTAGAAAACAA1350.10805013526276191No Hit
TTAACTGGGAACGTGTGACTGAACTAGAATTTCTTTTGTTGGAGGGGGATTG1350.10805013526276191No Hit
GTACCCGGGACCATTTGACCCAGCCATCCTATTGCTGGGTATATACCCAAAG1330.10644939251812842No Hit
AGCTGTGGGCAGATTACAACGCCTGGCTAATTTTTGTATTTTTGGTAGAGAC1330.10644939251812842No Hit
CTAAGCGGGTGCTTAAGGGTAGGCATAGTGGGTGGCTCTACATTTGAGAGTT1330.10644939251812842No Hit
ACACATGGGGTTAACATGGTGGTAGACCATGGAGAATGGGTAGATGGAGCTT1330.10644939251812842No Hit
TACGTAGGGGTATTTCATGAATATTTATGTATACTCCCTGTACTTTCACCCT1330.10644939251812842No Hit
AGACTGGGGGTCTGATACCGTTGTTATAACAAAACAAATTTTTTTACTATAG1330.10644939251812842No Hit
GCTTTTGGGAACTAAGCTAATGATTCTTCCTTTTCTACTTTAGGAGAAAACT1320.10564902114581166No Hit
TTAGCAAGGACTTTTCAGTTAGGTAACTGTGTGATCCAGTTCTTCCAGCTGC1320.10564902114581166No Hit
ATTCTGGGAAGGAAGTTTCAAAGAAAATTTAAAAAAAGAATACTTTGAACTA1320.10564902114581166No Hit
ACATCTGGGATACTAAACAGGCTTGGGTTTGGCAGGACTCATGCTGCCTTCC1300.10404827840117814No Hit
TTGTTTGGGGGAGATAAGGGATGCACTTGGCATCCTTTTCAAACTCTATTTC1300.10404827840117814No Hit
GCTATAGGGGATCAAAAATTAGCTAATCAGATTAATGATCTCCGCCAAAGTG1300.10404827840117814No Hit
CTAAAAGGGCAGGTCTCACATGTAAGTAGTCCATGATCCTTTTTGAGTTAAT1300.10404827840117814No Hit
TGTTACGGGATGACAAAGAGAAATTAGTAGAAGCAGTATGGGCGAATATTAG1300.10404827840117814No Hit
TGACATGGGATATCAGATTATAGAATAAAGAACTCTGAGAACTCAGTAATAA1290.10324790702886139No Hit
ATCGCAGGAGTCTGCACCATCTTATAGCTGCCCAGCACACTCGACTGTTCAT1290.10324790702886139No Hit
TTTATTGGGATATTGCTATGGATATAGAAGAACTAAAAGTAAATTCTTTGCA1280.10244753565654464No Hit
GCTGCGGGATATACAATCAATGCTATTTATTGTACCTCTGGGCCTACTCTTC1280.10244753565654464No Hit
CTAACGGGGTCTTAGGAAGGCCCATAATACTTGTTTTTCTTACATGTGACTA1280.10244753565654464No Hit
GCGCTTGGGAGTTCCTTAGTTTACATATGTTTGTGCAATTATTTTCTGTACT1280.10244753565654464No Hit
CAATTCGGGGTAAAATAAAATGTATATGTAATATCTCATGTTGTTTCCTTTC1280.10244753565654464No Hit
GCAATCGGGGATGTTACACTAGAATCAGGTTGGAATTTGGTGTCTTATTGCT1270.10164716428422789No Hit
CTTCTAGGGAGGACTATCTGGTGATGAATCTATCCACTAGAAGCTGGTAAGT1270.10164716428422789No Hit
TGTAATGGGATATTTCAAATACAAAGAAAAGTAAGAAATGAGTTTTCGTATT1270.10164716428422789No Hit
ATTAAGGGGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAA1270.10164716428422789No Hit
GTATAAGGGATCACAAAAAAGGGAGACTTTAATTTTGGAGGAAATATGGGAT1260.10084679291191112No Hit
CCGAATGGGATAATCACAATTTTAAAACACACAGTCTTCTCTACACCTTCAC1260.10084679291191112No Hit
GGCTTGGAGGGAGCTAATCTAACATCACCTGACTCTTACCATTGTACTTACC1250.10004642153959437No Hit
ATAGCTGGGCAGTCTTGGATGATCTGAAAACTTTTAATTATCTTCTTTTTTA1250.10004642153959437No Hit
ACCAGGGGGCCTTTTTTATGATGGCGAATGAGGCAAAGAGGGCTAAGCCGAC1250.10004642153959437No Hit
TAATGGGATCCATATGTTTTGGACAAAAGGCTGGTTCCATAAATATACTGGA1250.10004642153959437No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)