FastQCFastQC Report
Sat 31 Dec 2022
SRR5849438.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849438.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124713
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGCCGTCT16301.3070008740067194Illumina Paired End PCR Primer 2 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCTCGTATGC14081.1289921660131663No Hit
CTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCTTATAC2380.19083816442552098No Hit
GCTTTTGACCCAAAGGTCCTGACGGTTGCGTCCGGGGGAGGGGATGGAAGGG2260.18121607210154514No Hit
TAGCCGGGATTGTGTGCCAAGTAATCTGTGCTAAATAAAAATAGAGTATTTG2220.1780087079935532No Hit
AAGCCTGGGAACTCCTGAACGGCGCAAGGGCAGTTTAGCTGATGTTGTTGAC2140.1715939797775693No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAG2130.1707921387505713No Hit
TAGATAGGGCATTGTGGGAGTTGCTGATACTGGGGAAATGATGGCAGATCTG2040.16357556950758942No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGATCCTGTCTCT2010.16117004642659546No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGA2010.16117004642659546No Hit
AAATTTGGGCTTGCTCAGACATTAGCCTTTGTACTATTAAGAACTTTAGCTG2000.16036820539959748No Hit
CAGCGCGGGTATAAAGAGAAACAATATGTAAATGCCCATGATTAAAATTGCT1980.1587645233456015No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACAATGACAGAACTCGTATGC1970.15796268231860353No Hit
ATCAGGGGAACAGAAAGAAATGAAGTGACTCAGCTTCTGGCTTCTCTGCTAC1970.15796268231860353No Hit
GCAAAAGGGCTTCCGATCTACTTCTGGGCACCTGTGCCAGTAATCCCCCAAC1910.1531516361566156No Hit
TAGTGCGGGATTCATAGTAGGAAATTTAGAAAATAGAAAATGATAAAAACAA1900.1523497951296176No Hit
TCATGTGGGCCAATACTCATTTAATTAAGATTTAATCATTTGCTAATGGAAA1890.15154795410261962No Hit
GACAGGGAATTAACTTTCATATTAGCAGATTAAACTAGCAGAGCAGGTTCAA1890.15154795410261962No Hit
TCTGCGGGGGTTGGAAGACTATTATTAATATTAATTGGGGACAGGGCATTGC1890.15154795410261962No Hit
CATCATGGGAACTGGGTTTTAAAAATCTGGTTCTAACAGTAGTTTATGTCTT1880.15074611307562163No Hit
GCAATGGGACAGACTGGATCCTCAAGGACTACTTTATGAGCTTTTACTTTCC1860.14914243102162564No Hit
TTTGTCGGGAAGGTATGATCTCCAGAAGTATCTCTCAACTGCTTAAATTAGG1830.14673690794063168No Hit
GCTGTGGGGATGGAATTCATTGGAATGTGCTTAAAATGCTAAAGTGTATGTC1820.1459350669136337No Hit
ACACAGGGGTGATCAGAAGGATGGAAGTGCCATTGAGTGAAACTAGAAAGCT1800.14433138485963773No Hit
ATTCGCGGGCTTTCTCTCTTTTGTTTTGTTTGTTTGTTTGTTAAAGTTCATT1800.14433138485963773No Hit
GCTAAAGGGACAGGATATGAATTGAATTTAAATATTTCTAGGTTATGGGAAA1800.14433138485963773No Hit
CGTGGCGGGATTACAAAACTGAATGCAAATGTCCTTTGTGGTGCTTGTTCCT1790.14352954383263974No Hit
ATAATAGGGGTGACCACTTTTTCAATACTGTGAGATTATGGTTATGTGAAGA1770.14192586177864377No Hit
AACAAGGGATGTTACTAAGAGACAGTCTTTAAAGGTAATATCAAATTTCTTT1750.14032217972464778No Hit
TTGCATGGGGATATAATAAATACTATAAAATTGTAAAATAGGCTAATAAATA1750.14032217972464778No Hit
TAGCTCGGGGAAACGGAAGTGAGCGGCGGGGTCGACTGACGGTAACGGGGCA1740.1395203386976498No Hit
ATGCGTGGGGGATAGTTAAGGGAGGTTGTCTGAACAGGGATTATAATTAGTT1740.1395203386976498No Hit
CAAAAGGGGCCGATCTTGGTAGGGGAACAAAAGAAGTAAAAGATCTCTATAC1730.13871849767065184No Hit
AGTAAAGGGTCTGCAAGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAG1730.13871849767065184No Hit
AAACCAGGGACAAAACATTGCTGAAAGAAATCACAGGAGAACTAAATAAATA1730.13871849767065184No Hit
CAATATGGGTCTGAAGCGGGCTGTAACAACTAGTTTTAATGAGTAAAAAGTC1720.13791665664365382No Hit
GGAACGCTGGGGAATGAATGAATGAACGAATGAATGTACTATGAGCTGATAC1700.13631297458965785No Hit
AATTATGGGATTGACTCTGAGCAACACAAATGTGGTCTGTTTCAAAGTGAGG1690.13551113356265987No Hit
AATCCAGGGATATTGATAAATGGGTTGAAGTCTATTTTTCCATTATCATCTT1680.13470929253566188No Hit
TGGATTGGGAGTGGACTATTATTTTGTGCATCAAATTCTTAAAAACGAAAAT1680.13470929253566188No Hit
ATCAGGGGATAATAGAGAAGGGAGTTTTATGGAAGTTTCTTTGAAGATTTTT1680.13470929253566188No Hit
TCTAATGGGAGACTTTGAATTAAGGGAATGTTGTAGCTGGTTTGATCTTCTA1670.1339074515086639No Hit
TAGCTTGGGGAGCTGTCCTATTAAAAGACAGAGACTGTCAGATTAGATTTTT1660.1331056104816659No Hit
TGTCTAGGGAATTGACAAAATTATCAGGAGATCTCAATCAACAATCAATGTA1660.1331056104816659No Hit
TAAATAGGGATCCCATTTGTCAATTTTGGCTTTTGTTGCCATTGCTTTTGAT1650.13230376945466793No Hit
TTCACCGGGATGGGAATGCTCTTTAGAATTCATTTTGTTCAGCCCCTTTGTT1650.13230376945466793No Hit
AAAAAGGGAGGGCTTACAGAAATGAATAAGTGGGTGGCACTAAATTATGGGA1650.13230376945466793No Hit
GTGTTCGGGATATTGGACTTGTTTTTTCCTGCTGAGGAAATCTAATTTATGA1640.13150192842766995No Hit
AGGTAAGGGAGTTCTTACACCTTGTTGGAGAAACTACAAAAGAAAGTTGTCC1640.13150192842766995No Hit
CATCACGGGGAATATAAAATACATACCTATACAATTTGAGAAGCCACTTGAG1640.13150192842766995No Hit
TCTGGAGCGGGAAGTGATTTCAGTCAAAACTGGAAAACAACTAAGATGTAGT1630.13070008740067193No Hit
CATCAGGGGGACACTGACGTTTTGTCTCTTGTTCCCCAGCCCCCAGCCCATG1630.13070008740067193No Hit
ACATCTGGGATACTAAACAGGCTTGGGTTTGGCAGGACTCATGCTGCCTTCC1620.12989824637367395No Hit
CTTAGCGGGATATACATTTTTACTCTTGTTGCAAAAATAAACTGTTTCTTGC1620.12989824637367395No Hit
GGGCACGGGAGAACTAGAAATCCCATTTGACCCAGCAATCCCATTACTGGAT1620.12989824637367395No Hit
GGTGCAGGGGATCTGTCACCGGGGTATATGGGGACACTGCCAGTCACCAAGG1610.12909640534667596No Hit
ATCGCTGGGATCAATTAGAGGCTCATAGACTATATTAGGGACAATCTCAGGG1610.12909640534667596No Hit
AATGTGGGGGGGGGAGTGGAGAGGGGTTACATAACAGTATTGTAGAAAACAA1610.12909640534667596No Hit
GATTGAGGGCATTTCAATAGACACAGAAAAAGCTTTTGATAAAATCCAGTAT1600.12829456431967798No Hit
TTAGCAAGGACTTTTCAGTTAGGTAACTGTGTGATCCAGTTCTTCCAGCTGC1600.12829456431967798No Hit
GCGCTTGGGAGTTCCTTAGTTTACATATGTTTGTGCAATTATTTTCTGTACT1600.12829456431967798No Hit
AACGTAGGGACTGGTACCCCAGGAGGATGCCTTGTAACTGCTCTTTGGTCAT1590.12749272329268No Hit
GTAGTTGGGCACTGCGCTGGTCCCTGCTCAGTTTATGGAATCATTTCACTGA1580.126690882265682No Hit
TACGTGGGGGAGTCGCCGCGGGACCGGGGCCGCTGGGGTCTGGACGGGGGTC1580.126690882265682No Hit
AAAAGCGGGGGTATTTTTTCATTAATAAAACCATCTTTGAAACATTCAACAT1570.125889041238684No Hit
AGTGCTGGGATTTTCTAATTGCCATCAAATGTCTATGACCCTCAGAAAGCAT1560.12508720021168604No Hit
GCCGACGGGAGTTGAAAAACTGAAGACGCTGCCTAAAATACAAGCTGAGTTA1560.12508720021168604No Hit
CGTGCTGGGGTTTGTAGAGTGCAGGAATAGTATTATACATATGAAAACACAA1540.12348351815769006No Hit
GTGGTGGGCAGGCCACATACTGGGAGAAAATATTTCCAAACCACATATCCAA1540.12348351815769006No Hit
CGTGCTGGGTATGTATGTATAGCTTGAGTATTGAGAGATCTCTTAGAAATGC1540.12348351815769006No Hit
GTGGCCGGGATCAAAATGTGCTTTGAAGGAAACAGGCCTAATCCTAAGGTAC1520.12187983610369409No Hit
AGTTCCGGGGGTAGCCAAGGTTGAGAACCAGTACCGTACGTACAAAAAAGTT1520.12187983610369409No Hit
TATTCCGGGGTATTTTCTTTTTCATGATTATCGGTGACTGGTCAGTGTACTC1510.1210779950766961No Hit
GTATTTGGGATTTTTCTACATGCTCTTTAGGGTTTTAAATGTCAACCTACTG1510.1210779950766961No Hit
ACAGCAGGGAGAGAAGCTTCATCTATGATGAGCCTAGCAAAGATACTCTCTC1510.1210779950766961No Hit
TCAAACGGGAACCTGGGTTAGGAAAGATGGATAGAGATAAAAGAGAAAAGAA1510.1210779950766961No Hit
TATTAAGGGATTGCTTTCCATAAAATAAAGTGTCCTTTTTGTCTCGTATAGC1510.1210779950766961No Hit
AGCTCAGGGATTGAAGGATAATTTCTTAGGGTACAGAATTCTTGGTTGGTGT1510.1210779950766961No Hit
TCGACTGGGTCCCCATCAAAAGACATATATTAGTGGATTGTATTAAAAAATA1510.1210779950766961No Hit
TAATGGGATCCATATGTTTTGGACAAAAGGCTGGTTCCATAAATATACTGGA1510.1210779950766961No Hit
CTCATTGGGATCCAGTAGCTAACATTAGATCTAGCTTATTATTTCAGAAATT1490.11947431302270012No Hit
CTAGATGGGTCAGCCAAAAAATTATTTCCCAGCAGAAGAATCTTTTCCTACC1480.11867247199570215No Hit
TGACATGGGATATCAGATTATAGAATAAAGAACTCTGAGAACTCAGTAATAA1480.11867247199570215No Hit
CTAACAGGGGCAAATCCCTAGTTTGTATCCTGTGGATTTAATTTCTAACAAT1480.11867247199570215No Hit
CTAGGCGGGATTTTTTACCTGTTCCTGTCAACTTCTGTTTTCCAACTCACCT1470.11787063096870415No Hit
TCAGCAGGGCTTGAGGAGTGTTAGCAAAATTTAAATTTTGACCCTGGCCATT1470.11787063096870415No Hit
AACGCAGGGATGCCATATATACAGACTCCAGTGTGGTTATTTTCTCTTCTTA1470.11787063096870415No Hit
AATGCTGGGCTACTGAGTGGCAGAGCCAGGGTCTGGGCCCAGCCAGTGTGGC1470.11787063096870415No Hit
AAGACTGGGGTGGAGGAAAGATTATGAAAGGAACAGGCTCTTTAAAAAAGAA1470.11787063096870415No Hit
GTGAATGGGATAAAACAAGGACAAATGTACACAGTGTGTAAAAATGAATAAT1460.11706878994170615No Hit
TAGCCAGGAATGAAATTCCTTTCAAAACCCCAGCAAGATTTTTTTGTAGATA1450.11626694891470817No Hit
ACGTTTGGTATGAAATAATGAAATTGAAATGAGATGAGATGAGATGAAATAA1450.11626694891470817No Hit
TTAGTAGGGACAGTGATTTATATGTTACTTTTTAATATTCATTTTTTGCTTT1450.11626694891470817No Hit
TCGCTTGGGGTGATGATGGTGCGGTCATATTTTATAGGGATAAAACAGAAGA1450.11626694891470817No Hit
TTACTGGGATCAGAGCAGAAACAAATGAAATTAAAGTGAAAAAAAAGTGCAA1440.1154651078877102No Hit
CTCGCAGGGGTACAAATTATTTCATCACCCAGGTATTAAGCCTAGTACACAT1440.1154651078877102No Hit
ACTTGCGGGTGAGATACAGAACTTTATTTTCTTTTTAAGCAAACATGAGCTA1430.1146632668607122No Hit
GCTGCAGGGATATCCAGTGTTAAGAGTTTTAGGAGAATGTTGCTAACATTTG1430.1146632668607122No Hit
GGGTCAGGGAAGAGGAGCAGTGAAATGTATATAGTTGATGTTCAGTATTTCC1420.11386142583371421No Hit
ACATTTGGGTAGGAAGGAGAAAACTCAGGAGGGAGGAGACCAACTTCAAAGA1410.11305958480671621No Hit
ATTTAGGGGACATGGGATAGTTTATGTGATTCCTCTATAAGCCAGGACCTAA1410.11305958480671621No Hit
ATTCTGGGAAGGAAGTTTCAAAGAAAATTTAAAAAAAGAATACTTTGAACTA1410.11305958480671621No Hit
TTCAATGGGAATTCAGTTTTTCATAGCAAGTGAATAAATTAGCATTCTGGCA1410.11305958480671621No Hit
GTACTTGGGAGGGTGGTCCCAACCATGTGATGTCTGGAGCATAGGATGCATT1410.11305958480671621No Hit
CTCATAGGGATTCTCAAGGAAACACCATGTTGTCCCACAAAATTAGTCTCAA1400.11225774377971823No Hit
CTCTTTGGGGTTGGTGGGTGCATTTTAATTTTTTCATTCCCTGGACTATGGG1400.11225774377971823No Hit
TAGCAGGGGAGAATTTTAGGGACCAGAAGCAAACTGGTAATTCTCTGCATTT1400.11225774377971823No Hit
ATTTTGGGGATATTTTGTCCCTGAGACTGTGAAGTGAGTTGAATCTGTATAA1390.11145590275272026No Hit
CAGTATGGGAGGAGTTCAAGATGCAGAGATGGCAAGATTCCATTTCTACAAA1390.11145590275272026No Hit
ATCTACGGGACTCAAAATAATTTGATACTAAAAACTGAATTCCTGTAGCACA1390.11145590275272026No Hit
CTCACAGGGAAGCTAGGAGAGACTGAGAATTTTAATCAGTTTGGGCATATAG1390.11145590275272026No Hit
TTAACTGGGAACGTGTGACTGAACTAGAATTTCTTTTGTTGGAGGGGGATTG1390.11145590275272026No Hit
AAAATGGGGCTGCTGATTTTGCTTGTGGCCCTTAGCATCAGATGGCCTTGAG1380.11065406172572226No Hit
CGGACCGGGCGATCTTTGTAAGGGGTGAACAGTTTCTGAACAGAACACTTTG1380.11065406172572226No Hit
CAAGCCGGGGATCTTTGGTGGGGTCTGCATACGGGATCTCTTCCTAGATTAT1380.11065406172572226No Hit
AGATAAGGGGTCTCTTAGGTAACTTCCTGTAAACGATTTGGAAATAGGATGT1380.11065406172572226No Hit
GACACTGGGATGTTTGGTTGATGCGAGCAGCTGCACTGCTCATGCAGTTAGC1380.11065406172572226No Hit
TCTGCCGGGGAGTTTTTCTATGCAGGGAGTCATTCACACATCCAGCAAGGAA1370.10985222069872427No Hit
ACACATGGGGTTAACATGGTGGTAGACCATGGAGAATGGGTAGATGGAGCTT1360.10905037967172627No Hit
CTAAGCGGGTGCTTAAGGGTAGGCATAGTGGGTGGCTCTACATTTGAGAGTT1350.1082485386447283No Hit
GTAAGAGGGATATAAATTTGAGGAAGGCACGATTTCACTCATAACAATCTTA1350.1082485386447283No Hit
CTTCTAGGGAGGACTATCTGGTGATGAATCTATCCACTAGAAGCTGGTAAGT1350.1082485386447283No Hit
TTGTTTGGGGGAGATAAGGGATGCACTTGGCATCCTTTTCAAACTCTATTTC1350.1082485386447283No Hit
GAATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.1074466976177303No Hit
TCTAGTGGGAGTCTTTTAAAATTGAGAGTCCTAGGCCTACAATTGTCTTTCT1330.10664485659073232No Hit
TTGTCTGGGGTTAGAATTTGGGCAACATTTTGATTATAATGACAACTTCATT1330.10664485659073232No Hit
CTAACGGGGTCTTAGGAAGGCCCATAATACTTGTTTTTCTTACATGTGACTA1330.10664485659073232No Hit
GGATACGGAGTGATGAGAAGTGATTGATCCCTAGTTCCTGGTCATCAGCCTA1330.10664485659073232No Hit
GCAATCGGGGATGTTACACTAGAATCAGGTTGGAATTTGGTGTCTTATTGCT1320.10584301556373432No Hit
ATTAAGGGGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAA1320.10584301556373432No Hit
AGACTGGGGGTCTGATACCGTTGTTATAACAAAACAAATTTTTTTACTATAG1320.10584301556373432No Hit
ATTGCTGGGAGATCAAAGACTTAAGTCTAAGACCTGAAACATAAAGATTCTA1310.10504117453673635No Hit
ATTGCAGGGTATTGAGGGATTTTTTATTTTCAAATGTTGCAATTTTATATAA1310.10504117453673635No Hit
GACTGTGGGACTCTGAGGTCCAAATACTGTCTGTCAATGTGTAACCTGATGT1310.10504117453673635No Hit
GCTGCGGGATATACAATCAATGCTATTTATTGTACCTCTGGGCCTACTCTTC1310.10504117453673635No Hit
TTCCGCGGGATTTGCTAGGCAGTGAAATGGTTTCTGTTTTACACAATGAGGA1300.10423933350973837No Hit
GTACCCGGGACCATTTGACCCAGCCATCCTATTGCTGGGTATATACCCAAAG1300.10423933350973837No Hit
TAGTGCGGGACGTGGACAGTTCTTATGCCTATCATGTCAAGACCTAGCTCCT1300.10423933350973837No Hit
TTATGCGGGATATTTTACTTTGTTGCAGATAGTCTTGCCGCATCTTGGCAAG1300.10423933350973837No Hit
TCTAGGAGGGAGAGATGATTTGTTTTGGAACATAGCACAAGTGTTACAGGTT1300.10423933350973837No Hit
ATCGCAGGAGTCTGCACCATCTTATAGCTGCCCAGCACACTCGACTGTTCAT1290.10343749248274037No Hit
CGTAGCGGGATACTTTGCTAATTTGATCATAGCACCTAGATGGATCCTTATG1290.10343749248274037No Hit
TGAAAAGGGATGGGTCAGTTGTCAAGAATGTTCTTTGGAACACATTGTATAA1290.10343749248274037No Hit
AGGCTAGGGATCGACAGACACTTTAAATATCTCAGAGTTAAAAGTTGCAAAT1290.10343749248274037No Hit
TAAGCCGGGACTGTTATTCTTTTCTGTACCAATTCCATTTTAACTTCTACAG1290.10343749248274037No Hit
GCCAAAGGGATGGTCATTTTAGATATTGATTTCTTCTTAAATATAATGAAAA1280.10263565145574238No Hit
GTTTACGGGGTGCTCAGATGGAAAGTCTGAGCTGAGGTTGGTCTCTTGCCAA1280.10263565145574238No Hit
TACGTAGGGGTATTTCATGAATATTTATGTATACTCCCTGTACTTTCACCCT1280.10263565145574238No Hit
GAATAGGGAATACATAATTCAAAAATTGTGAAAGTTTATTAAAATGTAATGC1270.10183381042874441No Hit
TGTAATGGGTCTCTTCTGGACTATATAAATTACATATGTAGTTAATGACAAA1270.10183381042874441No Hit
AAACTGGGGCGATCTAGGGCGGGATAAAGGTTTTAGGTATTTAGGGAGTTTG1270.10183381042874441No Hit
TTTTTAGGGATGGTACAGATATTTTGCTATTGTTTCATTAGGCTGTGAACTT1260.10103196940174641No Hit
CAAGCCGGGATAGAAGATAGTTTTGTTGAAAATGATTGTAGGTTTATACTTT1260.10103196940174641No Hit
AAGCCAGGGTGACTATCTTATGTCAGGACCTTCATGTGCCAAACGTCAGTGG1260.10103196940174641No Hit
TTTGCTGGGAAGATGGAGGAAGGCGGGAACCTAGGAGGCCTGATTAAGATGG1260.10103196940174641No Hit
GAAGTAGGGGATCTACGTTGGGGTCGGGCCATTGGATTTTTTCCATTATGTT1260.10103196940174641No Hit
TCGAGGGGGTTACACATAGATGTTCTTAAATATTACCTATATAAGAATGGTA1250.10023012837474841No Hit
AAAACGGGGCCACTATGAAGAACAGTTCGGAGGTTCCTCAAAAGGCTGAAAA1250.10023012837474841No Hit
AGCTAGGGGACAAGGGACATTTGACAGTATCTAGACACAAACAACTAGAGCA1250.10023012837474841No Hit
AAAGTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.10023012837474841No Hit
CCGAATGGGATAATCACAATTTTAAAACACACAGTCTTCTCTACACCTTCAC1250.10023012837474841No Hit
TAATCTGGGGTAGCACAGCACACAAAGGAATGGTTAGAAATGATCAATACCC1250.10023012837474841No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)