FastQCFastQC Report
Sat 31 Dec 2022
SRR5849431.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849431.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57689
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4240.7349754719270571No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3230.5598987675293383No Hit
TGATTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA820.14214148277834596No Hit
GTTAATGGGACACTAGTCAAATTGATTGGCATTTCCATTTATTTTCCATTGT710.12307372289344588No Hit
GGCTATGGGTTATATGCAGTAATTGTATCTTAGCCCAGTTTCTCCAAATCCT640.1109396938757822No Hit
ATATCCGGGACTCATTGAGTGTTTATTATGAAGCATGTTTTATAGTAGGTTT640.1109396938757822No Hit
GTGCTTGGAGCGTTGAACATTCCTAAACACGGAGGAAGTAAATAGCGGTTGC630.10920626115897311No Hit
TCTGCAGGGGTCTCTTTAGGAAAACATGAGCAGTCCTGGAAGCCCTGGACAA630.10920626115897311No Hit
TGTAGCGGGACACCTTTCAAAGAATAAATGAAAAATAGAGAGACATACCTGG620.10747282844216402No Hit
TGCGCAGGGGATCTTGTATAGGGAACTACTTAAAAAATATAGTCAATAGGTT610.10573939572535493No Hit
TCTAAAGGGACAGCTTAAAGTTTAGTTTAAAAGTTGTAGGTGATTAAAATAA610.10573939572535493No Hit
ATTATCGGGAGAAATTCAAAATAATGACCTTAAGGAAACTCAGTGAGATACA610.10573939572535493No Hit
TTGTGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC610.10573939572535493No Hit
GCTTTGGGGGTCAAATTGTTTACAGGTTATTTTTGTGCGTAATTGTGATAGC610.10573939572535493No Hit
GTGTGCGGGATTCAGTGAAGTGTGGGAAGTAAATCTCCTGAGTCCCTGATCT600.10400596300854582No Hit
ACCTCAGGGATCACTTGCAAACGTGAATATTGCTATTGCCTCAGGCTACCTC600.10400596300854582No Hit
CCTCGTGGGGACTTAAAAATCGAGTACAGGTGATGAAATCTTGGCAGTGTTA590.10227253029173673No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCCTGTCTCTTATAC590.10227253029173673No Hit
CTCGGTGGGGAAGAAGGAGAGAGGGGAGAAGAGGCAGGAGCTGGAAAGGAGA590.10227253029173673No Hit
GAACTAGGGCGATCTATTTCAGGGAAGTTATCACTCACGACAGTTACTATTT580.10053909757492761No Hit
CATATCCGGGACCTCCCTGGCAGTGCGTACATAATGAGGCAGCTTGCTCCAG580.10053909757492761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)