FastQCFastQC Report
Sat 31 Dec 2022
SRR5849430.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849430.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57774
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4340.7512029632706754No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC2860.49503236750095203No Hit
TGATTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA740.1280852978848617No Hit
TGCGTAGGGGAGGGAAGTCAGGAAGGAAAGAAGGAAGAGAAGGCCGCAAAAC730.1263544154810122No Hit
TTAAATGGGGAGAGAAAGAGGGAAAGAGAGAAAGACAAGAAAGGAAAAAAAG700.12116176826946377No Hit
GGCCATGGGACAAGAAGGAATAGAAAACTGATACTGTTTTAAATAATCTGTA680.1177000034617648No Hit
CCTCGTGGGGACTTAAAAATCGAGTACAGGTGATGAAATCTTGGCAGTGTTA630.1090455914425174No Hit
AGTACTGGGATCTGGCGCTTTGCTGGCAATTTCGAGAGAACCGTGGGCACCG630.1090455914425174No Hit
TATAAGGGGTTCATGGTTAGACTCCATATAATAGAGCTTCAGATGTCCAAGG630.1090455914425174No Hit
TAGTAAGGGCAGGCGTAAATATTCTGATGGTTAATTGTAGACATCTACTTGA610.10558382663481843No Hit
TCGTCGGGGCAGCCAGTAAGTTAGGGGTTCAAACCCACAAGACCTTATGGCC610.10558382663481843No Hit
GGCTATGGGTTATATGCAGTAATTGTATCTTAGCCCAGTTTCTCCAAATCCT600.10385294423096895No Hit
GTTAATGGGACACTAGTCAAATTGATTGGCATTTCCATTTATTTTCCATTGT590.10212206182711947No Hit
ACTGATGGGACGAAAGTTCCCTCTCATGACCCCTTCTTAAGAAGGGACTTGC590.10212206182711947No Hit
ACTACAGGGGGCCTTTGCCGGCCGCCGCGAGGACGGGCAGGGCACGCACTGG580.10039117942326999No Hit
TTCGTTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG580.10039117942326999No Hit
TTAGCGGGAGAGCAGTACATATTCAAATGTATTCAGAAAGTCAAAAGATTAC580.10039117942326999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)