FastQCFastQC Report
Sat 31 Dec 2022
SRR5849429.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849429.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57927
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4080.7043347661712155No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3050.5265247639270115No Hit
TATAAGGGGTTCATGGTTAGACTCCATATAATAGAGCTTCAGATGTCCAAGG840.1450100989176032No Hit
TGATTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA790.136378545410603No Hit
TCTGCAGGGGTCTCTTTAGGAAAACATGAGCAGTCCTGGAAGCCCTGGACAA770.13292592400780293No Hit
GGCCATGGGACAAGAAGGAATAGAAAACTGATACTGTTTTAAATAATCTGTA730.12602068120220278No Hit
AATCAGGGAAGTTGATGAAAGGGGACTGGTTTAGCACAAGCCATCCACATTA710.12256805979940269No Hit
CCTCGTGGGGACTTAAAAATCGAGTACAGGTGATGAAATCTTGGCAGTGTTA690.11911543839660262No Hit
AAAATCGGGCTGAGAAGAGGGACAGGATTGAGAAGGTTCTGAAATCGTCGCA690.11911543839660262No Hit
AATCCAGGGGCCCCCGCCTGTCCGGGCGCCGCCATGGAGCGCATCGAGGGGG660.1139365062924025No Hit
TGCTGCGGGAGCGCAGAGATTGAGCTTCTGCCTACCAACGACAAGAAGAAAT650.11221019559100247No Hit
TAGTAAGGGCAGGCGTAAATATTCTGATGGTTAATTGTAGACATCTACTTGA630.10875757418820238No Hit
TTCGTTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG610.10530495278540232No Hit
TTAGCGGGAGAGCAGTACATATTCAAATGTATTCAGAAAGTCAAAAGATTAC610.10530495278540232No Hit
GCTAAAGGGTGGCTTTGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTAC600.10357864208400226No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCCTGTCTCTTATAC600.10357864208400226No Hit
GTTAATGGGACACTAGTCAAATTGATTGGCATTTCCATTTATTTTCCATTGT590.10185233138260223No Hit
GACGCAGGGGCTTCTCCAGGGGCAGGGCTCCTACCCCCACTGTGGGGGGCGG590.10185233138260223No Hit
TTAAATGGGGAGAGAAAGAGGGAAAGAGAGAAAGACAAGAAAGGAAAAAAAG580.1001260206812022No Hit
AGTACTGGGATCTGGCGCTTTGCTGGCAATTTCGAGAGAACCGTGGGCACCG580.1001260206812022No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)