FastQCFastQC Report
Sat 31 Dec 2022
SRR5849410.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849410.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58650
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC4070.6939471440750213No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3160.5387894288150042No Hit
CAGCCTGGGTGATACAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGAA1010.1722080136402387No Hit
CAGTGCGGGGCTGGAGTTCTCTATTTACCCGGCGCCCCAGGTTTCCACAGCT890.1517476555839727No Hit
CACACTGGGGCAGCTGGGATTACAGGCACACGCCACCACACCTGGCTAATTT830.1415174765558397No Hit
TGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA830.1415174765558397No Hit
AGTTACGGGGCTTCTAGGCGGACTATGACTTAGTTGCGTTACACCCTTTCTT800.13640238704177324No Hit
AAAGAAGGGGACCAGGGGATTTTCCAAGGAGAGGTTGCTATGGTGACAGACT790.13469735720375106No Hit
TAGCCGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGAC780.1329923273657289No Hit
CGGATTGGGAGAATAGTACAAGGGTTAGGTGGGGTAGATTTAATGTTTCAAA770.13128729752770674No Hit
TTGCGTGGGTCTGCTACAACTTCTGAGGCTAAGGGAACAAAGGAAAGAGGAG760.12958226768968456No Hit
GCAACCGGGGAATAATAAAGACTAAGGGGAAAAAACCCAACAACCTTTAGAT750.1278772378516624No Hit
TAAGCCGGGATACCAAATGCCTGCTGCTACCACCCTTTTCAATTGCTATCTT740.12617220801364024No Hit
TAAATAGGGCCACCTCAACCTCCCAAAGTGCTGGGATTACACGCATGAGCCA730.12446717817561807No Hit
TTTGTTGGGATGGTTTCCAAACTGTATCGTAGCCGTCCACAAAGATGTAATC720.1227621483375959No Hit
CCGTCGGGCGAGGGGCCCGTGGTGGGATGCAGGAGCAGCCGGCATCCCCACG710.12105711849957373No Hit
TCCACCGGGGTGCCCCAGCTTGGCTCCTTCCTCCACTTCCTCCCTTCTCTTT710.12105711849957373No Hit
AAACTGGGGTTGCCAGATATATATTGAGTGCTGATTTTATGTCAGACACTGT710.12105711849957373No Hit
TCATAGGGGAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTTT710.12105711849957373No Hit
GCAAATGGGATGGAATTTGGCCTTCTAGATGCCTTGAGTACATTTTGATAAA700.11935208866155159No Hit
ATCACCGGGACTACCACAATCCTTGACGTCTTTTGTTAGGCTTTTTCTTTTT700.11935208866155159No Hit
GTACTTGGGAGATCTAATCATTTACTTTTGCCCAACAGCAGATAATGAAAAG700.11935208866155159No Hit
AGAGTGGGATGCATAAAGAATCATCCCTCAAAGTGCTTACATTCTAATAGAG690.1176470588235294No Hit
TGTAAGGGGACATATTCCCTAATATACTATCTTGTTTTGTCTTACAAACACT690.1176470588235294No Hit
CACATTGGGTCTTCCGATCTATGACAGGGGCTAAACCTAGCCCCAAACCCAC680.11594202898550725No Hit
GATGCTGGCGAGGACGAGGAAGGTGCGGGCCAGGGGTTTCATGCGGCAGGTC680.11594202898550725No Hit
TGTCAAGGGTAGCTGTTTTGAGGAAACTCAAAGAAATTCAAGATAACACAGA680.11594202898550725No Hit
CACGACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC680.11594202898550725No Hit
GCTTGTGGGCGATCTGGACAGCGGGTCAATAAATATTTCATTAGTTTACATT680.11594202898550725No Hit
GAAATTGGGCATTAATGTGAACATGCTTATTAAAAGTTAACCTCTTTATAAA680.11594202898550725No Hit
GCGTTCGGGCATGTGTCAGATCTGCATGCATTGCTTGCATTTTTCTGGTATC670.11423699914748509No Hit
TGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG670.11423699914748509No Hit
ATCAGGGGACCTCTTAAAGAAAACCCACCCCGTAGATTCCCATCATACACTT670.11423699914748509No Hit
GCTGTTGGGTTAAATTTGCACTGTTACATTTTGGTTCAGTTTATTTCCATGT660.11253196930946291No Hit
CTTCCTGGGGAACTTGATGGAGCATGGGCAGATTTCAGTGATACGAGAAAGG660.11253196930946291No Hit
GTAAACGGGACTCGCGCAGACCTAGCGCGTCCAGGTGGGAGGTTAGTGTGGC660.11253196930946291No Hit
ACTGACGGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCA660.11253196930946291No Hit
TAAATGGGGCAATCCTAGTCTCTGATAAATCAGAGTTTAAACGAACAAAGAT650.11082693947144075No Hit
TACGCAGGGGACGTCAGGCAGGCTATGCCCTTCCGTGGTTAATTTCTTCCCA650.11082693947144075No Hit
GCAATGAGGGAGGGCCCCAGCAGATGGGGCTGGGTCAGGAACCATCTCTGTC650.11082693947144075No Hit
CAATATGGGAAGCATTCATTTTCTACCTGTGTGGAACTTTAATTAGTTTAAG650.11082693947144075No Hit
ACATGAGGGGAGTACGTGGACCATTGCTTGCATGTTAACTTTTTGTTTTGTT650.11082693947144075No Hit
CACTCGGGGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT650.11082693947144075No Hit
CTCGCTGGGTTCCCAGGAGAACTGCTGATGCTGAGATGCTTGGATCCTACAA650.11082693947144075No Hit
CATGTCGGGCAGTAGTTCTGAGGTTGAGAAATTCTACCTATAAGCAAATGGA650.11082693947144075No Hit
TTTGTGGGGGGGACACACCTTCAGCTAACTTTGCTTTTTCATGCTTGTTTTC650.11082693947144075No Hit
CTAGTAGGGGACTTCTCCAGCTGCTGGTATAAAAACAGTTGATCCTCTGCCT650.11082693947144075No Hit
TACCGCGGGAAATAAAGAAAAATGAAGAAGCTGAGTCTCCAAGGGGTAAAAT650.11082693947144075No Hit
TAATTGGGGGAAGAGAAGTGTTAATAAAGAGAACATCCGACACACAGAAGAA640.10912190963341858No Hit
TTTACAGGGTGCTGAAAGAAATCACAGATGAAACAAACAAATGGAAACACAT640.10912190963341858No Hit
TGAGGTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC640.10912190963341858No Hit
CGCAGGGGGAGGAAGGAAGAGACGCAGGCAGGCTGCGGTTACCCAAGCGGCC640.10912190963341858No Hit
ATAATCGGGAGCTTAATATAGAATTAGGTCATTCATAAATCTGTTGCAATTA640.10912190963341858No Hit
AGTAATGGGATCTACGCTGGGGAAAGGATAATTCTTATTTTTACCCTTACAG630.10741687979539641No Hit
GTTTATGGGTCCCTTCTCAGTCTATTCACTCATAGGCACATTTAACCCTTTA630.10741687979539641No Hit
ATTGCAGGGAATGAAGCAGCAACTTCGAAAGGCGATGTTGGAAAAAGACGGA630.10741687979539641No Hit
TTCTCAGGGATTGGAAGGCTGAGGCGGGTAGATCACCTGAGGTCAGGAGTTT630.10741687979539641No Hit
AAAGCTGGGATTGTGGCAATTACTCTGCATAGCCTAGCAGTGCTGGTCTCTC630.10741687979539641No Hit
TCTTGTGGGTTACAGTTAAAAATTATTTTCTACTATCACATTTGGGATTAAG630.10741687979539641No Hit
ACGCATGGGATCACCAGGAGGAATTCTTTCTTTATGGTAGAAAAGAACATCA630.10741687979539641No Hit
TTGCAGGGCAGATTTGGCAGAGAGTATAATGAAGAATCTTAGGCGGGTGCAC630.10741687979539641No Hit
ATTTGGGATGGATTGTTTAACCTAAGCAAAAAATATTGATTTCCTTTTCCCT620.10571184995737425No Hit
AACAAAGGGAGTGATGAACCATAAATAGAATTGACAGACTGACTTAATGAAA620.10571184995737425No Hit
TACATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC620.10571184995737425No Hit
TTATTTGGGTCCTCAGAAAAATCCTCAAGGGTATAAGCATCCAAAGCAGTTC620.10571184995737425No Hit
TGGACCGGGGTTTCAGGTATGTCCTTATTAGTAGCATGAGAACAGACGAATA620.10571184995737425No Hit
GTTCTGGGGCGATCTGCCCCGGGGATCTGTCTTTCTGGCTTGTTAGATCTTC620.10571184995737425No Hit
GCGTCAGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAG620.10571184995737425No Hit
TGCCATGGGTCTTTACTGGAGTGAGTGCGGGAGACAAACAGTTTACAACAAC610.1040068201193521No Hit
GACACTGGGAATGATGCAATTAGAAAGTGGTCCGGATTTGGGTGGCAATGGA610.1040068201193521No Hit
ACAATAGGGAAGTCCCTGATATTAAAAACAGCATATTTGCATATAATCTAGG610.1040068201193521No Hit
TCTTAGGGGCTTCCGATCTTCTTACCGCCGGGCAGTGACTAGTGAGAGAAAA610.1040068201193521No Hit
CATGCGGGGATTCATAAATAGGAGAAGCACTGCCCCCTGAACTTGCAGAACT610.1040068201193521No Hit
CGCGAAGGGGAGGGGAGGGTGGATAGAAATGGGAATTAGGAAATATTTGTTC600.10230179028132991No Hit
TAAATAGGGGGTTGGTTGGTTTTTTTCATTATAAAGCAATCCCTAATAAAGA600.10230179028132991No Hit
CACTGTGGGATACTCTTCAATCAGCCACATAGCCCTCGTAGTAACAGCCATT600.10230179028132991No Hit
GCATGTGGGAAGAAACTAGGAAACAGGAAACACAGTTTTGGCCACATAAAAT600.10230179028132991No Hit
TTTGCTGGGATTCCTCTCATTACTAGTATGATTAAGTCTGTGTTTAATGACC600.10230179028132991No Hit
GGCTATGGGGGCTTATGAAAAAGAAAACGCAATTGGATCTGCTGCTGCCATT590.10059676044330776No Hit
ATTGGAGGGCGATCTATGAAGGGGGACGCTCTTCCGATCTGTTTTTGGGTAC590.10059676044330776No Hit
CACATTGGGCGATCTGAAAGAGGGCAGAACAACGAGTCTCATTTTTATTTGC590.10059676044330776No Hit
CTTCGAGGGACCAAATGTGAATGCAGCAATATTAAAAAAGGTCATTCAGTGG590.10059676044330776No Hit
TACGATAGGGGTGGTGAACAGATTTCAACTGTGATGCAAATTCTTATTCATT590.10059676044330776No Hit
TCCGATGGGGGGTTTATCGTGTGAGCACACCATATATTTACAGTAGGAATAG590.10059676044330776No Hit
GGTCTGGGGGGAAAATACCTGATATAAATTCCCTCATCCTTTAAGACTCTGA590.10059676044330776No Hit
ACAGCTGGGGTACATTAGTGCTGGTGCCCTCATTTTATAGCTGAGGTTAGGT590.10059676044330776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)