FastQCFastQC Report
Sat 31 Dec 2022
SRR5849409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57997
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3840.6621032122351156No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3190.5500284497474007No Hit
CAGCCTGGGTGATACAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGAA1010.17414693863475697No Hit
TGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1010.17414693863475697No Hit
TAAGCCGGGATACCAAATGCCTGCTGCTACCACCCTTTTCAATTGCTATCTT910.15690466748280082No Hit
TAGCCGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGAC800.13793816921564908No Hit
CATTGCGGGCGATCTACGTGCGGGAAGAAGAAAAAATTTTCTAACATTGTGA800.13793816921564908No Hit
GCGTTCGGGCATGTGTCAGATCTGCATGCATTGCTTGCATTTTTCTGGTATC760.13104126075486663No Hit
ACGCATGGGATCACCAGGAGGAATTCTTTCTTTATGGTAGAAAAGAACATCA760.13104126075486663No Hit
AAAGAAGGGGACCAGGGGATTTTCCAAGGAGAGGTTGCTATGGTGACAGACT750.129317033639671No Hit
TCATAGGGGAAGCTGAAATATATGCATGTAAAAACTTTGACATCTTTTTTTT740.1275928065244754No Hit
TTGCGTGGGTCTGCTACAACTTCTGAGGCTAAGGGAACAAAGGAAAGAGGAG740.1275928065244754No Hit
TGAGTGGGGTTATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCA730.12586857940927978No Hit
TAAATGGGGCAATCCTAGTCTCTGATAAATCAGAGTTTAAACGAACAAAGAT720.12414435229408417No Hit
CGACGGGGCACCCCGAGGTGTTCCTCAGATTGAAGTCACTTTTGACATTGAT710.12242012517888856No Hit
AACGGGGAGATGAAGAAGTTGATGAAATGATCAGGGAAGCAGATATTGATGG710.12242012517888856No Hit
CCGTCGGGCGAGGGGCCCGTGGTGGGATGCAGGAGCAGCCGGCATCCCCACG700.12069589806369295No Hit
GAAATTGGGCATTAATGTGAACATGCTTATTAAAAGTTAACCTCTTTATAAA700.12069589806369295No Hit
GCAACCGGGGAATAATAAAGACTAAGGGGAAAAAACCCAACAACCTTTAGAT690.11897167094849734No Hit
TAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG680.11724744383330171No Hit
CATGTCGGGCAGTAGTTCTGAGGTTGAGAAATTCTACCTATAAGCAAATGGA680.11724744383330171No Hit
TGTAAGGGGACATATTCCCTAATATACTATCTTGTTTTGTCTTACAAACACT680.11724744383330171No Hit
CAGTGCGGGGCTGGAGTTCTCTATTTACCCGGCGCCCCAGGTTTCCACAGCT680.11724744383330171No Hit
GTACTTGGGAGATCTAATCATTTACTTTTGCCCAACAGCAGATAATGAAAAG680.11724744383330171No Hit
TGCTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA670.1155232167181061No Hit
CCGAAAAGGGACGTATTGACTTATTTTCTTTTTCTCCTTAGGAACTGAGAGA670.1155232167181061No Hit
AGTTACGGGGCTTCTAGGCGGACTATGACTTAGTTGCGTTACACCCTTTCTT660.11379898960291049No Hit
TCCACCGGGGTGCCCCAGCTTGGCTCCTTCCTCCACTTCCTCCCTTCTCTTT660.11379898960291049No Hit
GTGCTAGGGGTGGACTTTTTCTCAGAAATTATTAAAGCCAGAAGAGAGTGGA660.11379898960291049No Hit
TGACGCGGGGTTTTCATTTTCTATAATAAAAAGTTAAAAAGGAAAAAGACAC660.11379898960291049No Hit
ACAGAAGGGGAAACAAACCCATGCTTGAGACTCTCCTTGCTGGTCCAGAGCC650.11207476248771488No Hit
GTAGTTGGGATATAAGAAATGTATGCACTGTTTGAAATGTAAATTATTCTTA650.11207476248771488No Hit
AAAGCTGGGATTGTGGCAATTACTCTGCATAGCCTAGCAGTGCTGGTCTCTC650.11207476248771488No Hit
CAACCTGGGTCTCTCTGCCTGTGGTCCTTTTCCTCCATTGTATTGGCTCTGG640.11035053537251927No Hit
AAGCGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA640.11035053537251927No Hit
TTCTCAGGGATTGGAAGGCTGAGGCGGGTAGATCACCTGAGGTCAGGAGTTT640.11035053537251927No Hit
CGACAGGGGACGTCACTTTGTTGAAATAATAGGTAAGATGTAAACAGGAACT640.11035053537251927No Hit
GCATGTGGGAAGAAACTAGGAAACAGGAAACACAGTTTTGGCCACATAAAAT640.11035053537251927No Hit
TGCACAGGGTATTTACTGTTATATACTGTAAGGCCTTCTTTAGGCCTTTAGC630.10862630825732365No Hit
AAGCAAGGGGGCTGATAACCAAATTTTAACACATCAGCATTCACCACCAGGT630.10862630825732365No Hit
TAACACGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC630.10862630825732365No Hit
GTAGAGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC630.10862630825732365No Hit
ATGCTAGGGGTCACTGTGTCTATGTAGAAAGGGAAGACATAAGAGATTCCAT630.10862630825732365No Hit
CTTTTTGGGTCCCATTGCTGTGAATGACCATGTTGGCCATGCTGTGAATGCA630.10862630825732365No Hit
GATTACGGGCGATCTAGAGATGGGAGACTGTGTCAGTCAACTAAACATTCTA630.10862630825732365No Hit
GCTTGTGGGCGATCTGGACAGCGGGTCAATAAATATTTCATTAGTTTACATT630.10862630825732365No Hit
GATGCAGAGGAGGAAGCAGCTACCCGAATACCTGCTTAGTTCCTGAAAAGGG630.10862630825732365No Hit
TGCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG620.10690208114212804No Hit
GTTTATGGGTCCCTTCTCAGTCTATTCACTCATAGGCACATTTAACCCTTTA620.10690208114212804No Hit
CACTCGGGGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT620.10690208114212804No Hit
CTCGCTGGGTTCCCAGGAGAACTGCTGATGCTGAGATGCTTGGATCCTACAA620.10690208114212804No Hit
ATCACCGGGACTACCACAATCCTTGACGTCTTTTGTTAGGCTTTTTCTTTTT620.10690208114212804No Hit
GGCCATGGGGATCTGCTGCAGGGATATTTTGTAGATTATGGTTAAAGGGGGA620.10690208114212804No Hit
GCCTCCCGGGGTGCTGGGATTACAGGCGTGAGCCGCCACACCCAGTCCTTCC610.10517785402693243No Hit
CTCTCGGGGGGACTGCTTTGAGTGGAGATGGCACTGAACTCGTTGTAATAAC610.10517785402693243No Hit
TGTCAAGGGTAGCTGTTTTGAGGAAACTCAAAGAAATTCAAGATAACACAGA610.10517785402693243No Hit
TCGAGAGGGGGGCCGCCTTGGTTTTGTGGGTGCTCCTACGTCACTAGGCTGT610.10517785402693243No Hit
AGAGGGGATTTGCTGCCAGTAGCGTAACAGTCATATTTGCTTTTATTCACAT610.10517785402693243No Hit
TCTAGAGGGCAACCGACTAATCACCACCCAACAATGACTAATCAAACTAACC610.10517785402693243No Hit
TTTGTGGGGGGGACACACCTTCAGCTAACTTTGCTTTTTCATGCTTGTTTTC610.10517785402693243No Hit
CTAGTAGGGGACTTCTCCAGCTGCTGGTATAAAAACAGTTGATCCTCTGCCT610.10517785402693243No Hit
TTGCAGGGCAGATTTGGCAGAGAGTATAATGAAGAATCTTAGGCGGGTGCAC610.10517785402693243No Hit
TGAACTGGGAGGTTAAAGAACTAACATTTTCTGAACCCTTCATTAACTTTTT610.10517785402693243No Hit
GTTATGGGGCTTTTGGTATTACAGGTATCTGAAACAAAGCACCTTCAATTCT600.10345362691173682No Hit
GCTGTTGGGTTAAATTTGCACTGTTACATTTTGGTTCAGTTTATTTCCATGT600.10345362691173682No Hit
GTCCAGTTTTCCAAAAATCCTTTTTCCCTGCTATCAGAAATGTGAAACCAAA600.10345362691173682No Hit
TTTACTGGGGTGTGGTAAGGGTGGCGCTTCCCGAAGGCCCCCTTCCCTTCGT600.10345362691173682No Hit
AACAAAGGGAGTGATGAACCATAAATAGAATTGACAGACTGACTTAATGAAA600.10345362691173682No Hit
GAATCTGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT600.10345362691173682No Hit
TACGCAGGGGACGTCAGGCAGGCTATGCCCTTCCGTGGTTAATTTCTTCCCA600.10345362691173682No Hit
GTTGTGGGTCATGAGAACAGCATGGCAGAAAGCGCCCCCATGATTCAATCAC600.10345362691173682No Hit
CACACTGGGGCAGCTGGGATTACAGGCACACGCCACCACACCTGGCTAATTT600.10345362691173682No Hit
TTAGCAGGGACTTGAAAATACAGTTGGTGCACTGGAGAATCTATTATTTAAA600.10345362691173682No Hit
TTAGCCGGGCGATCTGGGCTTTGTCATGGTGTTTTACGTTCGTTGTTGTTTG600.10345362691173682No Hit
GTCGCGGGTGATTCTTCTAGGTGTATGTATGGATCTATGACCCAGTTCACTT600.10345362691173682No Hit
TCAGTAGGGTAAAAAATTAGTTTAAATTTGCCAGCAGGGAAGTAAAATAATT600.10345362691173682No Hit
AGAGACGGGAGATATCGATGGTATTTTGGAAAGAGGTGCAAAGAAGACTGCA600.10345362691173682No Hit
GTCCCGGGGGAAACTAACACTTTGCTAGAAACTTAGAAAATGTTATTGCATT600.10345362691173682No Hit
CACGACTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCC590.10172939979654119No Hit
GACACTGGGGCTTTTCGAGGTAGGAGTCGACTCCTGTGAGGTATGGTGCTGG590.10172939979654119No Hit
TTGATAGGGATAGTCCACACCCTGAAAATGTATTTCTTCCAGAAAGTCTGGA580.10000517268134558No Hit
TCGTAAGGGGCCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGG580.10000517268134558No Hit
AGGGTAGGGACAGTTATTTTGTAGAACGTTTCTTAATTTGTCTTTGCCTGAA580.10000517268134558No Hit
GACACTGGGAATGATGCAATTAGAAAGTGGTCCGGATTTGGGTGGCAATGGA580.10000517268134558No Hit
GTCACAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC580.10000517268134558No Hit
TTGATAGGGAACCATTATGGAAAACAGTATGAAAGATTCTCAAAAAACTAAA580.10000517268134558No Hit
AAAACAGGGATGAAAACACTGTTTTTCCCCCTAATAAAGCATATTTTACTTT580.10000517268134558No Hit
AGCCAGGGGCATAATGAACATTCAAGTACAAGTCTTTGTGAGGACATCCTCT580.10000517268134558No Hit
TGCGCAGGGGAGGAATGATGACAAGAAAAGGCCGAATTGCAGTGTCTCCATC580.10000517268134558No Hit
GGTCTGGGGGGAAAATACCTGATATAAATTCCCTCATCCTTTAAGACTCTGA580.10000517268134558No Hit
ACTGACGGGCCTCCTATTTATTCTAGCCACCTCTAGCCTAGCCGTTTACTCA580.10000517268134558No Hit
GCGTCAGGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAG580.10000517268134558No Hit
TTGAGCTGGGATCCATCAGAATGTCTAACTCTTCCTCGGTGCTTCCTTTGCA580.10000517268134558No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)