FastQCFastQC Report
Sat 31 Dec 2022
SRR5849407.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849407.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53234
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3500.657474546342563No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3080.5785776007814555No Hit
TACCACGGGGCAGTCTTGCCATTCTTAACTTATTGTGACAATAAAATTTAAT960.18033587556824585No Hit
GTAATAGGGGATAAGTGTTTGCTTTTACTATTATACATAAAGTTATTGTCAT890.1671863846413946No Hit
GTCTTGGGGAAAGTTTGGAAATGTTTTTCGTTTTTGTCATAAAAGAAACATG870.1634293872337228No Hit
TAGCCAGGGAACACTCAAGGTTAAATTGCATGTTGTTATTAGAATCAATGTT830.15591539241837923No Hit
TGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA800.15027989630687155No Hit
CTCGAAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG800.15027989630687155No Hit
TTAACCGGGGAGGCAAGTGTGGGAAATGTGTGAACCCACGCTCATCCAGTCT800.15027989630687155No Hit
ATGACAGGGATGATTTGGCTCTGTGTTTTCCCTCAAGTCTCATGTTGAATTG790.14840139760303567No Hit
TGCGACGGGATAATAGCTCTGCTGCAGTTCTGTGACTTCCAAGCCAGGAATT780.14652289889919975No Hit
CCTGCCTCAGCCTCCTGAATAGCTGGGATTACAGGCACCCGCCACCATGCCC770.14464440019536387No Hit
GTACATGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC760.14276590149152796No Hit
ATTACCGGGGTGGACCCTGCTTCTTTCCTAAGTACTACATTGGGAAATGTTT760.14276590149152796No Hit
TCGTTGGGGTCGGGACCGTGGGCCTTCCCTCCATTTCCCACAAGCGGTATGC750.14088740278769207No Hit
CGCATAGGGAACCAAGTAAATTTTATTACTGAAATGCAAGGATGGTTCAGCA750.14088740278769207No Hit
CCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG750.14088740278769207No Hit
TGCTCAGGGGCCTTGCTTCCTGCTTCGCCTCCGCGCCTCGCGCTATGGGACA730.13713040538002028No Hit
CGCATTGGGGTTAAGAGACACATTTATAGCACTAAATGCCCACATCAAAAAA730.13713040538002028No Hit
CTTTCCGGGATGCCTAGAAAAATCTGCCTAGGCCTTTTACAAAAGCTGTCTT730.13713040538002028No Hit
TGAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC720.1352519066761844No Hit
ACAGCTGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGT710.1333734079723485No Hit
TGAAATGGGGCCGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAGG710.1333734079723485No Hit
AAACAGGGATCCCACAATGCAGAGACTTACCCACAAAGCAAATGATTACCTC710.1333734079723485No Hit
AACACAGGGGGGGTTTGAAGCCGCGCCGCGAGGGAGCGAGGTCGCAGTGACA710.1333734079723485No Hit
GTTGCAGGGGGCCCACAGTTCCGACGAAAAATGGCGGGGTCTCCTGAGTTGG700.1314949092685126No Hit
GCTCCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA700.1314949092685126No Hit
CTCGCAGGGTATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCC700.1314949092685126No Hit
CACCAAGGGATTAAAAGAAGCAATTATGAATAATGGAAAGTAAACAAATCTA700.1314949092685126No Hit
ACTCCAGGGAGATCCACATTCAAAAATTGCTTTGTATTTCTATATACTAGCA690.1296164105646767No Hit
ATTCTTGGGCGGCGACAGAGGACAGCCAGAAGCCCAGCCAGCCCCAGACTCC690.1296164105646767No Hit
GGCGCGGGCAACAAGAAGTGTTCAAAATATTTTAAATATTTGTGCATTTTAT690.1296164105646767No Hit
AGTACAGGGCGGCAGTATTGTTTTCTTATATGTGCAATGAAGTGGAATGATA690.1296164105646767No Hit
TCAGGTGGGAGGTACCAGAGTCAAAGGATGGTACAATTTGATAAAATCATTT690.1296164105646767No Hit
GTCTTTGGGACTGTTCCTCTATAAACTCAACTTGAATAATGGTTATTCCTTC680.12773791186084082No Hit
TCAGCTGGGGGAGCAAGGGCTCAGAAATGGAAGCTCAATTTTAATTCAGGAT680.12773791186084082No Hit
CACCGCGGGACGGCGGCCGGGCGGGGACACGGTTCGGGGGCTCCGGGCTGGG680.12773791186084082No Hit
GTGACAGGGACATCATCTGCTTCCCTGTAATGAACTATGAGGGTTCTTTATC670.1258594131570049No Hit
CTCCAGAATTCCCTAGATCCAAATTGCTCTTCTCCTTGGCCTTTTGTGCTTC670.1258594131570049No Hit
TATCTGGGGAGGAAGGTAGTGACGTCATAGGTAAACAGGCTCTGTATCCGTG670.1258594131570049No Hit
CGCACTGGGGTTCCTGTTTCTTCACATCCTCTCGAGCACCTGTTGTTTCCTG670.1258594131570049No Hit
CAATAGGGGGACCCTCGGCTCCCGAGGCCCCGCGGCGCCTCCTTCTGCGGAC660.12398091445316904No Hit
ATAAACGGGGGATTTTCTGGTTCAAAATTTTACTGGCTTTCAGAAATTCTAA660.12398091445316904No Hit
GTTCATGGGGAGCTTGCTGGCATGGTGGGCAGTATTCCAGGAGAGGCCATGT660.12398091445316904No Hit
TTGCTAGGGGTTGGAAATGTATTTGAAAAAATTCTCACTTCTGCAAGGCCAG660.12398091445316904No Hit
ACTGTTGGGGAGTGTAATCAAGAAGAGTATTCTGTACTTGACTCTTCTTATC660.12398091445316904No Hit
CAATTGGGAGCATTCAAATTTGACAGGAGGGTTGGACCTTACTACACTGAAT650.12210241574933313No Hit
TGTTCAGGGCACCTTGATCAGGTAAAGAAAAAAAATCTAAATCCAAAATTTC650.12210241574933313No Hit
ACGCACGGGGCTGGTCCCAAGTGGTTGCTGGATGTGACTTGGCAAGGAGTGG650.12210241574933313No Hit
GCAGTTGGGGGGGTAGCTTGGCCAGGTTGTGAGGAACCGCAGCGCGCCGCAG650.12210241574933313No Hit
TTCCTGGGGAGAGACCGAGGGGAGAGAGGTCGATATAGAAGATAATGGGCTT650.12210241574933313No Hit
GAAAAGGGATTCTAGCAGCATCCATTCTTGTTTAAGCCTTCCACTGTTAGAG650.12210241574933313No Hit
GGGGTGGGGAATTAAAGTTTGAAAATCAGAGTCTTTAAAGTATGTTTCTATT650.12210241574933313No Hit
AGATAAGGGGCATCTGGCCTGTTTCTTCAGAATAGATTTCTAATAATTACAT650.12210241574933313No Hit
GTGCCAAGGGTGTAAGAAACACCCTAACATTCTCTAACTTTTGAAAATGAAT640.12022391704549723No Hit
ATTGGTGGGGATATCTAGGTAGCTTACTTTGTGTTTGTCATTCTTGTTTGTT640.12022391704549723No Hit
AATGCAGGGGGGCTACAACCTGAGCCCCCAGTTCACCCAGCTTCTGGTCTCC640.12022391704549723No Hit
GCGTAGGGGAAGGCCCATCAGCGACAGCTGACCCCGCCTGAGGGCTCCTCAA640.12022391704549723No Hit
GTGGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC640.12022391704549723No Hit
ACTAGAGGGGAGCCTCCAAAGTGTCCCATGCTAGGGGAACTTCACGTGGGGC640.12022391704549723No Hit
CAATCGGGGATGATCATGCAAAATTAACCTTGCCTATTTCTGGGATGAAAAA630.11834541834166135No Hit
CGCGCTGGGCTGGTGAGCTCTGAGTAAGGTTAGTGTCTTCTAACTGTCTTAC630.11834541834166135No Hit
TCAAACGGGACACTATGAGAAAATGTTAAGATACATCAAGGATAAGAAAAGA630.11834541834166135No Hit
CGCATGGGAGCTCCATGAGAACAGAGACGATCCATGTCTGTCTGTATCTCTA630.11834541834166135No Hit
GGTCCGGGGCTGTTCCCTGGATTAAAAGAAGCCTTTGTTGTTGAAGAATGGG630.11834541834166135No Hit
CACCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAAGTTCCGCAT630.11834541834166135No Hit
AGTAACGGGTGATTAGATAATCTGAAAACCTTTCTTCTTCAAATCACCTAGA630.11834541834166135No Hit
TCAAAGGGGGTACTATAAAGCTACAGTAACCAAAACAGCATGATACAGGTAG630.11834541834166135No Hit
GTTTTCGGGAGAGTGGGGTTATAGATTTTTAGGAAGAATACCAGAGGTGAAG620.11646691963782545No Hit
GTCAATGGGCTCCTTCCCGGCGGCAGCTACCGAGAGTGCGGAGCGACCAGCG620.11646691963782545No Hit
TTGTTAGGGGGCACTGGTCATTTTTTAAAAGCCCCCAGTTGATTCTAATGTG620.11646691963782545No Hit
CTCAGTGGGCTGTGGGGATGGGAGGCATTATTATGAGAACACTCACTTTTCT620.11646691963782545No Hit
TACGCTGGGAGCCGCCCCGGGAGGAGCGCGCCCAGCAGCTGCTGGACGCGGT620.11646691963782545No Hit
CTAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC610.11458842093398955No Hit
GGCAATGGGATGTCTTTCCGATGGTGTCTCCCAAGTGTTGGTGCTTTGGGTT610.11458842093398955No Hit
GATTAGGGGCCCGTATGTTCTGAGTCTTGCTGTTTTTACCTGGTAATATTTA610.11458842093398955No Hit
GGCTATGGGATTCATTTATACACCATCAGCAGACATTGGCATCTTCAAAATT610.11458842093398955No Hit
AATACAGGGGTTTTCCCTCAGGGTCCAGTATGCCTTTGAGCTTTAGCTGTTA610.11458842093398955No Hit
ACACAAGGGCAGGATGTTTTGGGTCTCCCAAAGATCTATTACCTTGGGGAAA600.11270992223015366No Hit
TCTGCAGGGCGATCTGTAAGCGGGAAATGATAGCAGAATGTGCTTCTCTCTG600.11270992223015366No Hit
CCGTCTGGGGTCCAGGATCTCTATTGCTTCTTCATGCCTTAATATTTGGAAA600.11270992223015366No Hit
ATATATGGGGTCCCCTCTAAGACTTTAATTTTGTGTTTACTCTCTTATTTTT590.11083142352631777No Hit
TATTGCGGGATGGAGTCACTGATTCATCACTTTAAGTTGTATACTGAGGGCT590.11083142352631777No Hit
TTACCTGGGCTGCAAGTTGATTCTCATTAATTGTCAGATTCCACTACACTTT590.11083142352631777No Hit
GGTACAGGGCATGAACGACTGAATTAGAAATGGAAATGCCTGGCACAGCCAG590.11083142352631777No Hit
GCATTTCCTTTCCTCCAGGAATACACAGTGAAACTAAGTAATATCCCTACAA590.11083142352631777No Hit
TCTCTGGGGAACTGGAACCATCTGATGAAGAATCCTCAGAAGAAGAGGAAGA590.11083142352631777No Hit
GTTTGTGGGCAGATGAAGTTCAGTGGTTTCAGGGGGAGGAAATAATTTTTAA590.11083142352631777No Hit
TAGCTGGGGATATCAAAACAGTCCTTTATACTGTTTTATAAATGAGGAAACG590.11083142352631777No Hit
GAACATGGGATGTTTATTTAATCCATAACAGAAATGAGTAAGGGTAGCACAA590.11083142352631777No Hit
CTAGTTTAGGGACAGAAATTAACTTGGAGAGCCTGAGAAATTCCCAGTGGCC590.11083142352631777No Hit
GGCAACGGGCATGCCCAGAGCAGCCAGCAGGTGGCACCATCGGTCCTGTGAG580.10895292482248187No Hit
GCCAACGGTAGACCTTGAATGAGTCCATGTTACCGTGTGTGTCTGTATATCT580.10895292482248187No Hit
ATAAAGGGGCCCTTGGATTATGTGGTTTCACCTCAAATCAAAGAAGAACTGT580.10895292482248187No Hit
AATCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC580.10895292482248187No Hit
ATCCGCGGGATCATACGTCTTTTAGATCTTCTGGAAAAGAGACTTGAGAGAG580.10895292482248187No Hit
TGGGCAGGGAACCTTGAGACGAATGAGATGCAGACAGGCAGCACTGGAGAAT580.10895292482248187No Hit
TCAATAGGGCCAGATGGATTCTCTGATGAATTCTACTAAACATTTAAGGAAG580.10895292482248187No Hit
CGATATGGGATCTTCACAAAGGATTCACCAGTCAAAGCTCTTACTTGTGATG580.10895292482248187No Hit
CTAGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.10895292482248187No Hit
CGCTTTGGGCTAGGAATCTTTTTCTTCTCATAGCGAAGTCAAAGCTCTCTCT580.10895292482248187No Hit
GCTACTGGGGAGTCCAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTT580.10895292482248187No Hit
CACTATGGGCTGTTCTCTAATTATAGTATTCAAAAGAGACATACAATTTTAA570.10707442611864598No Hit
AAACGCGGGTACTTGAGGCCGGGAGTTTGAGACCGGCTTGGGCAATGTGGTG570.10707442611864598No Hit
TTATTAGGGAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGTGAAGCT570.10707442611864598No Hit
TGCGGGGAGGGAAGGAGGAAGGGAGGGAAGCAACGGTGGAAAAGCTAATGAC570.10707442611864598No Hit
GCGAATGGGCACTCTGTCTCTACCGACATTTCTCTTCTAAGAGAAGTAGTAA570.10707442611864598No Hit
GTGACCGGGTTTCTTAGTATATGAAAGGACAGGGATTTTTGTTCTTGTTGTT570.10707442611864598No Hit
TCTAACGGGTATCTCAGGCAGATTTATCTCTTTAAAACAAGTGTGTAGTTCA570.10707442611864598No Hit
GCAGAAGGGCTGAGAAAATCAACAATTCCATAAAAGCAACAAGACCAAGCAA570.10707442611864598No Hit
ATACTGGGGTGGGGAGAGGAGGACATTTGGGCTCACCTGTCAAGGTGGCCCT570.10707442611864598No Hit
AAAAGCGGGCGATCTAGGCATGGGAACTTTCAAAAGCTTCTGAGTGTCCAAT570.10707442611864598No Hit
GCACGAGGGACTCAAAAGCAGGAATTTTTAAAGGAATCTTTCATACAATTGG570.10707442611864598No Hit
GCGAACGGGAACTCTCAAACATTGATGGTGGAAGTGGGAATTGGTACAACTT570.10707442611864598No Hit
TTGATGGGGTACTGAATTCCTTTTTTAAATAATCTTTTGAAACTAAGAAAAT560.10519592741481008No Hit
TTGTCAGGGACTTACTTTCGGTCCAGCATTATGCTTTACATACTTAAATTTC560.10519592741481008No Hit
GTTTAAGGGAAACTTGAATAGTCTCAATGTTGTAGAAGATAGGCATTGTATT560.10519592741481008No Hit
AATGAAGGAGAAAGCTCAGCTTGAAAAAACAATAGAAACACTGAGAGAAAAT560.10519592741481008No Hit
AGGTGGGAGACATGGCACATGGATGTGATCTTCAAAAACTGTTACAGCCTCA560.10519592741481008No Hit
ACATACGGGTGCATTAAAAGTCTTCATCTAAACTGTTTGCAAAAGGATAGTG560.10519592741481008No Hit
GGCCTTGGGATCTCTCCTTTCTCATCATTTTCACTAAATCAGCGTAATGACT560.10519592741481008No Hit
CGTGGCGGGAATTTCTATGTTTCAGAACCTATTTCTTACAGTTTTTCTATGC560.10519592741481008No Hit
TAGTGGGATCACACAAGATAAATTTAGATTTCAGTCAGTACATTTTATTTCA560.10519592741481008No Hit
TGTAAAGGGACTTGAAAAAAATGAATGTATTTTTTTCTCCAAAGAACAGCAT560.10519592741481008No Hit
TGAGATGGGATTGCAAAGTAGGCATGTTGACACGTGTGTTGGGTGACCTGTT550.1033174287109742No Hit
GCCTGTGGGGGACACTTAATATTTTCTATATGTCATCAACTTCAACTAGGCT550.1033174287109742No Hit
GGGTACGGGAAGGTTCCATGGTCTCTGAGCCTGTAAAGAATCAAGGTTTAGT550.1033174287109742No Hit
TATGCGTGGGGACTACCTGATGCAAAAGCTATAAAATAAACAGTGGGAAGGG550.1033174287109742No Hit
GTCATTGGGGATTTGTATTTTAATCTCTCACCTCTTTTTCTTGTATTTTCAT550.1033174287109742No Hit
TGCTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC550.1033174287109742No Hit
GGGCGCGGGGTGATGATACCACTCAATAGGGCATGCCACTACTTTTCTTAAG550.1033174287109742No Hit
GTGACTGGGGAACACAGGCCCAAAGTTTATTAAACCCCGATCATAACCTCCA550.1033174287109742No Hit
GGGCAGGGGGTCAGCCCTCTGATACACTTACATTAAATAATTAATGATTCAA540.1014389300071383No Hit
TTGCATGGGTTCATTGGCCTGGGATAACTTCCTTGCAGAGCATACTAGAATG540.1014389300071383No Hit
GTATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC540.1014389300071383No Hit
TGAGGCGGGGAAAAAAGTCATACTAATTTGCTTCTTTTTCAATGTAGTGCTT540.1014389300071383No Hit
ACAGTAGGGAACAAGCCTAACCATGAAGAGTTTTTGCTATGTTTCTGTAGTA540.1014389300071383No Hit
CGTTCTGGGAGGCATGTGTAGAAGGCAATTTATCAAACCTATTGCACTGCCA540.1014389300071383No Hit
CACCTTGGGACATGAAGAATTACAGCAAGACATGCAAAAGTTTAAGAATGAG540.1014389300071383No Hit
ATGCTGGGGTATCAAAGGAGAATTTTTAAAGCCTTAACAATGCCTACTTTCC540.1014389300071383No Hit
AATAGTGGGATGAGAAGAGCAGAGGCCACTCTCCCAAGCTTCATCTTCTGCC540.1014389300071383No Hit
TGCCAAGGGGAGCCTGTCAGGGTGGTGGTCCTAGACTTCAGTGGTGTCACCT540.1014389300071383No Hit
GTAAGAGGGAAGAATTGGAATTTGGAATTACTTTACCCACATTTAAAATGAA540.1014389300071383No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)