FastQCFastQC Report
Sat 31 Dec 2022
SRR5849406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53390
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3440.6443154148716987No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3410.6386963850908409No Hit
AAAAGCGGGCGATCTAGGCATGGGAACTTTCAAAAGCTTCTGAGTGTCCAAT1030.1929200224761191No Hit
TACCACGGGGCAGTCTTGCCATTCTTAACTTATTGTGACAATAAAATTTAAT1010.1891740026222139No Hit
TGAAATGGGGCCGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAGG870.16295186364487732No Hit
GTAATAGGGGATAAGTGTTTGCTTTTACTATTATACATAAAGTTATTGTCAT870.16295186364487732No Hit
TGCTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC800.14984079415620902No Hit
GTGGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC790.14796778422925644No Hit
CACCTTGGGACATGAAGAATTACAGCAAGACATGCAAAAGTTTAAGAATGAG770.1442217643753512No Hit
GTCAATGGGCTCCTTCCCGGCGGCAGCTACCGAGAGTGCGGAGCGACCAGCG760.1423487544483986No Hit
CGCATTGGGGTTAAGAGACACATTTATAGCACTAAATGCCCACATCAAAAAA750.14047574452144596No Hit
ACTAGAGGGGAGCCTCCAAAGTGTCCCATGCTAGGGGAACTTCACGTGGGGC750.14047574452144596No Hit
GTCTTGGGGAAAGTTTGGAAATGTTTTTCGTTTTTGTCATAAAAGAAACATG740.13860273459449335No Hit
CTAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC740.13860273459449335No Hit
TGTTCAGGGCACCTTGATCAGGTAAAGAAAAAAAATCTAAATCCAAAATTTC740.13860273459449335No Hit
GACCAAGGGAACTGCTCAGTAAAACATTGCCATACATTAACTCTCCATTTCT740.13860273459449335No Hit
CAATTGGGAGCATTCAAATTTGACAGGAGGGTTGGACCTTACTACACTGAAT730.13672972466754074No Hit
TCAATAGGGCCAGATGGATTCTCTGATGAATTCTACTAAACATTTAAGGAAG730.13672972466754074No Hit
GTACATGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC720.13485671474058814No Hit
ACTCCAGGGAGATCCACATTCAAAAATTGCTTTGTATTTCTATATACTAGCA720.13485671474058814No Hit
GGCAAAGGGTAAGCTTTAAACTTCATTTTATGTCCTTTCACAAATAAATTAG720.13485671474058814No Hit
TTGTTAGGGGGCACTGGTCATTTTTTAAAAGCCCCCAGTTGATTCTAATGTG720.13485671474058814No Hit
TTAACCGGGGAGGCAAGTGTGGGAAATGTGTGAACCCACGCTCATCCAGTCT720.13485671474058814No Hit
TACGTTGGGAGCCAGGCAGAAGCCTTATTAATGAAAGCTGGAGTGCAGCCAG710.1329837048136355No Hit
TGGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA710.1329837048136355No Hit
ACATACGGGTGCATTAAAAGTCTTCATCTAAACTGTTTGCAAAAGGATAGTG710.1329837048136355No Hit
TTGATGGGGTACTGAATTCCTTTTTTAAATAATCTTTTGAAACTAAGAAAAT700.1311106948866829No Hit
CCTGCCTCAGCCTCCTGAATAGCTGGGATTACAGGCACCCGCCACCATGCCC700.1311106948866829No Hit
AACACAGGGGGGGTTTGAAGCCGCGCCGCGAGGGAGCGAGGTCGCAGTGACA700.1311106948866829No Hit
AGATAAGGGGCATCTGGCCTGTTTCTTCAGAATAGATTTCTAATAATTACAT700.1311106948866829No Hit
ATGCGGGCAGTGACACAAGTCTCTCAAAATGCATGTGGCAAATCTTTTTTTT700.1311106948866829No Hit
CGCATGGGAGCTCCATGAGAACAGAGACGATCCATGTCTGTCTGTATCTCTA690.1292376849597303No Hit
CTTTCCGGGATGCCTAGAAAAATCTGCCTAGGCCTTTTACAAAAGCTGTCTT690.1292376849597303No Hit
GTGACAGGGACATCATCTGCTTCCCTGTAATGAACTATGAGGGTTCTTTATC680.12736467503277768No Hit
ACACAAGGGCAGGATGTTTTGGGTCTCCCAAAGATCTATTACCTTGGGGAAA680.12736467503277768No Hit
AATGAAGGAGAAAGCTCAGCTTGAAAAAACAATAGAAACACTGAGAGAAAAT680.12736467503277768No Hit
ATTTACGGGGTTTTTTGAAATGATTGAGAACCATCCTCTCTCCACCAAGTTG680.12736467503277768No Hit
GTTGCAGGGGGCCCACAGTTCCGACGAAAAATGGCGGGGTCTCCTGAGTTGG670.12549166510582505No Hit
TCTAACGGGTATCTCAGGCAGATTTATCTCTTTAAAACAAGTGTGTAGTTCA670.12549166510582505No Hit
TTGCAGGGGAAGGAAAGAGTGAAAGATTGGGACTGCTTTGAGTGGAGATGGC670.12549166510582505No Hit
GCAGAAGGGCTGAGAAAATCAACAATTCCATAAAAGCAACAAGACCAAGCAA670.12549166510582505No Hit
TCGGTCGGGAATGAAATTCCTGTTTGAGTCCCACAGTGAGTAATTCTTATAA670.12549166510582505No Hit
CTAGTTTAGGGACAGAAATTAACTTGGAGAGCCTGAGAAATTCCCAGTGGCC670.12549166510582505No Hit
TCGTTGGGGTCGGGACCGTGGGCCTTCCCTCCATTTCCCACAAGCGGTATGC660.12361865517887245No Hit
TACGGTGGGAGGCAGAGAACTGAAGATACATGTATATCTCAAACTTGTGAGT660.12361865517887245No Hit
AATCGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC660.12361865517887245No Hit
CAGTCCGGGGACATTGTAAATTTGGAGGTTCTTTGAGACAGAATATACCCAG660.12361865517887245No Hit
TGGGCAGGGAACCTTGAGACGAATGAGATGCAGACAGGCAGCACTGGAGAAT660.12361865517887245No Hit
TTTGCTCGGGGTCATTAAATAGTAAATTTAAATTCCTTCCATTTAGTTTGGT660.12361865517887245No Hit
GTTTGTGGGCAGATGAAGTTCAGTGGTTTCAGGGGGAGGAAATAATTTTTAA660.12361865517887245No Hit
TCAAACGGGACACTATGAGAAAATGTTAAGATACATCAAGGATAAGAAAAGA650.12174564525191983No Hit
ATGACAGGGATGATTTGGCTCTGTGTTTTCCCTCAAGTCTCATGTTGAATTG650.12174564525191983No Hit
CTAATGGGGTGAAGCCAGCAATTGTTTCGCAATTCGGCATTTTGAAAACAAA650.12174564525191983No Hit
CCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG650.12174564525191983No Hit
TGAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC640.11987263532496721No Hit
GCTTTTGGGCATGTTTTACTCATATATAGCATCTGAGAACTAAAATGTGGTA640.11987263532496721No Hit
AATAGTGGGATGAGAAGAGCAGAGGCCACTCTCCCAAGCTTCATCTTCTGCC640.11987263532496721No Hit
ACTACTGGGGGCATTATTTCTAGCACATTACAACCAATCTTCATTCTCTATC640.11987263532496721No Hit
AAACGCGGGTACTTGAGGCCGGGAGTTTGAGACCGGCTTGGGCAATGTGGTG630.11799962539801462No Hit
ACAGCTGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGT630.11799962539801462No Hit
ATGGTGGGCACAAAAGCTTGATGCAGTTTTTTGACATAGCAGACTAAAGTGG630.11799962539801462No Hit
CGTTCTGGGAGGCATGTGTAGAAGGCAATTTATCAAACCTATTGCACTGCCA630.11799962539801462No Hit
CATAAGCGGGACAGTTTTACAAGGATCTCATGGCAGAACAGCGGAAGACTTG630.11799962539801462No Hit
GCGTAGGGGAAGGCCCATCAGCGACAGCTGACCCCGCCTGAGGGCTCCTCAA630.11799962539801462No Hit
CTAGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA630.11799962539801462No Hit
GGCAATGGGATGTCTTTCCGATGGTGTCTCCCAAGTGTTGGTGCTTTGGGTT620.116126615471062No Hit
TTACCTGGGCTGCAAGTTGATTCTCATTAATTGTCAGATTCCACTACACTTT620.116126615471062No Hit
AAGCCTGGGAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGC620.116126615471062No Hit
CGATAGGGGGTATAATTTGAAGTAACTCTCCATTAAGAATGAAATAGGCCCC620.116126615471062No Hit
GAAAAGGGATTCTAGCAGCATCCATTCTTGTTTAAGCCTTCCACTGTTAGAG620.116126615471062No Hit
CTCGAAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG620.116126615471062No Hit
GGGTTGGGAACAGCACTCTACCATCGATCCTATGTGGAATGTCCGCCACCTT620.116126615471062No Hit
TCAGCTGGGGGAGCAAGGGCTCAGAAATGGAAGCTCAATTTTAATTCAGGAT620.116126615471062No Hit
TAAAAGGGGGAAAAACACAAGACAGAATTGGAAAGCCTTAAGCATCAGCAGG620.116126615471062No Hit
CACCAAGGGATTAAAAGAAGCAATTATGAATAATGGAAAGTAAACAAATCTA620.116126615471062No Hit
CAATCGGGGATGATCATGCAAAATTAACCTTGCCTATTTCTGGGATGAAAAA610.11425360554410938No Hit
GCATCTGGGAACTGTAGAATTCTTCCTGTACGATTGGGGATATAATGGGCTT610.11425360554410938No Hit
CAGAGAGGGCGAGGAGTTAAAGGATTTGTCCGAGACCTTCAAGGTAACCCAA610.11425360554410938No Hit
CGGGCTGGGTTAAGTAAAACAAAGAAAAAACAGAAGTGGGAGATAGTTATTT610.11425360554410938No Hit
TAGCCAGGGAACACTCAAGGTTAAATTGCATGTTGTTATTAGAATCAATGTT610.11425360554410938No Hit
CACATAGGGACTCCTCTGTCCGCTCCCTGGCCTCTTTTAATGGCTACCCTGT610.11425360554410938No Hit
ATAAAGGGGCCCTTGGATTATGTGGTTTCACCTCAAATCAAAGAAGAACTGT610.11425360554410938No Hit
TGCGACGGGATAATAGCTCTGCTGCAGTTCTGTGACTTCCAAGCCAGGAATT610.11425360554410938No Hit
AACATCGGGGATTACATGTTTTTACAGTGGCCTGCTATTGAGGAAAGGTATT610.11425360554410938No Hit
AATACAGGGGTTTTCCCTCAGGGTCCAGTATGCCTTTGAGCTTTAGCTGTTA610.11425360554410938No Hit
TCAGGTGGGAGGTACCAGAGTCAAAGGATGGTACAATTTGATAAAATCATTT610.11425360554410938No Hit
ATCGTGGGGAAGGCTTTCCCACACTCAGTACATACATACTGTCTCTTTTCAG610.11425360554410938No Hit
ATTGCGGGGAAAGCAACCTGCATTGCCAGTGTGGGACCCTGGTCTCTGGTTC610.11425360554410938No Hit
GTAAGAGGGAAGAATTGGAATTTGGAATTACTTTACCCACATTTAAAATGAA610.11425360554410938No Hit
GTATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.11238059561715678No Hit
AAACAGGGATCCCACAATGCAGAGACTTACCCACAAAGCAAATGATTACCTC600.11238059561715678No Hit
GGTCCGGGGCTGTTCCCTGGATTAAAAGAAGCCTTTGTTGTTGAAGAATGGG600.11238059561715678No Hit
CTCGCAGGGTATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCC600.11238059561715678No Hit
GGCTATGGGATTCATTTATACACCATCAGCAGACATTGGCATCTTCAAAATT600.11238059561715678No Hit
TGTCAGGGGAACCCTCCCCACCTTGTTTTCCACTGGGAATGAGAATTTGATA600.11238059561715678No Hit
ATCCGCGGGATCATACGTCTTTTAGATCTTCTGGAAAAGAGACTTGAGAGAG590.11050758569020416No Hit
GCTATCGGGCCGAGATGCTGCCCGCTACCACTTCTTCCTCTACAAGCACACC590.11050758569020416No Hit
CGCATAGGGAACCAAGTAAATTTTATTACTGAAATGCAAGGATGGTTCAGCA590.11050758569020416No Hit
GTCATTGGGGATTTGTATTTTAATCTCTCACCTCTTTTTCTTGTATTTTCAT590.11050758569020416No Hit
CTCAGTGGGCTGTGGGGATGGGAGGCATTATTATGAGAACACTCACTTTTCT590.11050758569020416No Hit
TAACTAGGGAGTCTGAATGAAATGGAATGGGTCTCTAGCCTCAGTCTTGTCA590.11050758569020416No Hit
TTATAAGGGAGAGCTGAGCACTTTTGAAAAGCTTGTCCATCACTCTAGTAGG590.11050758569020416No Hit
GCTACTGGGGAGTCCAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTTT590.11050758569020416No Hit
ATAAACGGGGGATTTTCTGGTTCAAAATTTTACTGGCTTTCAGAAATTCTAA580.10863457576325154No Hit
GTTCATGGGGAGCTTGCTGGCATGGTGGGCAGTATTCCAGGAGAGGCCATGT580.10863457576325154No Hit
ACGTATGGGACCTTTGATCCATATTTGGGTGGCAGGAACTTTGATGAGGCTT580.10863457576325154No Hit
GATTAAGGGACTCTATATCCCCAAGTCCTAGAGCATGACCTGCATGTTGGAG580.10863457576325154No Hit
ATAAGTGGGCTAGAAAAGACATGAAGCTATGTTGAGGCCTGATAAGACCTTG580.10863457576325154No Hit
AATGAGGGGCAGTGGAATGCTGGAATCCTGATACAAATACTTGGACTTCTCT580.10863457576325154No Hit
CACCGCGGGACGGCGGCCGGGCGGGGACACGGTTCGGGGGCTCCGGGCTGGG580.10863457576325154No Hit
TATTATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA570.10676156583629894No Hit
TATGGTGGGCAGGCCTTTTAAAATTCTGTTCAGAACCAGAAGATGATCAAGA570.10676156583629894No Hit
TTGGCGGGGAGACAGGATTTACTTAAATCCATTGTTTCAAGAATGAAAAAGG570.10676156583629894No Hit
TGCATGGGGAGGGTATCTGCTCCTCTCTGTTCTTCAGTTTTTGTATCCTTTT570.10676156583629894No Hit
CACGTAGGGGGGCTGCTGCGGCCCAGCCCCGAGCCTGCCTCCCTCTTGGCCT570.10676156583629894No Hit
GGCATTTGGGTCAAGGTTTTATTAAAAGCTACATTTTATAACACTGGCACAC570.10676156583629894No Hit
CTTTCTGGGAAGAAGAATGATGATGGCTGGTATGAAGGAGTCTGCAATCGAG570.10676156583629894No Hit
AGTAACGGGTGATTAGATAATCTGAAAACCTTTCTTCTTCAAATCACCTAGA570.10676156583629894No Hit
GAGCTGGGGAGGCGCTCCGGGAACAGTGCATGTGCATGCATACCACAAGACA570.10676156583629894No Hit
CTTTGCGGGAGAACTTAAAGTATGATAATACGAAAAACAAGATAGGTATATA570.10676156583629894No Hit
ATATATGGGGTCCCCTCTAAGACTTTAATTTTGTGTTTACTCTCTTATTTTT560.10488855590934631No Hit
CACTATGGGCTGTTCTCTAATTATAGTATTCAAAAGAGACATACAATTTTAA560.10488855590934631No Hit
ATTACCGGGGTGGACCCTGCTTCTTTCCTAAGTACTACATTGGGAAATGTTT560.10488855590934631No Hit
TGTTCAGGGTGATAGGCAACATTGACTTTGATTCCCCAATTAAGTAAACTAT560.10488855590934631No Hit
ACTGTTGGGGAGTGTAATCAAGAAGAGTATTCTGTACTTGACTCTTCTTATC560.10488855590934631No Hit
CTTGTTGGGACTGAAGATTTATTCCAATTCTAGAATTGTTCTTTTTTATTTT560.10488855590934631No Hit
GGCCTTGGGATCTCTCCTTTCTCATCATTTTCACTAAATCAGCGTAATGACT560.10488855590934631No Hit
TGAGAGGGGGCTCATTGCAACCTCCACCTCCTGGGTTCAAGTGATTCTCCTG560.10488855590934631No Hit
GCCTGAGGGTCAGACTGATAAACTGCAGAAGGTTAGATAACACCAAACACAT560.10488855590934631No Hit
TACTGCGGGGTCTTCAGAAAACAGGCAGTAATGATTCTGATGTCCTTGTTCT560.10488855590934631No Hit
TATTGCGGGATGGAGTCACTGATTCATCACTTTAAGTTGTATACTGAGGGCT550.1030155459823937No Hit
TATGCGGGGATTTTAAAACTGAAACAATAACCAAATAACATTGGATAATAAA550.1030155459823937No Hit
GGGACCTTGCACCGGGACTGAATTAATGTATTCAATGGATCATTTGAACTTA550.1030155459823937No Hit
TGACGAGGGGTATATGAACCAAACTGAAGGCACTTCTAATGAGTGTTTCTAC550.1030155459823937No Hit
TTTAGAGGGGATCTGCAGCGGGAGAACAGAATACTTGTATGAAAATTCCCGA550.1030155459823937No Hit
AATGCAGGGGGGCTACAACCTGAGCCCCCAGTTCACCCAGCTTCTGGTCTCC550.1030155459823937No Hit
GAGAAAGGGGGAGGGATAATATGGTCTCCGGCGATGGACGCCCCAAAAGCAG550.1030155459823937No Hit
TTCCTGGGGAGAGACCGAGGGGAGAGAGGTCGATATAGAAGATAATGGGCTT550.1030155459823937No Hit
CACCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAAGTTCCGCAT550.1030155459823937No Hit
CGAAGGGGATTCATGTGAAGGTTAAATGTGAACACTTTTAACTTGGTAAATT550.1030155459823937No Hit
GATTAGGGGCCCGTATGTTCTGAGTCTTGCTGTTTTTACCTGGTAATATTTA550.1030155459823937No Hit
CTTGTTCCGGGGGAAGATTCTTACTGTTAACCAAATTTTGAGCAAGGAGTCT550.1030155459823937No Hit
AGTACAGGGCGGCAGTATTGTTTTCTTATATGTGCAATGAAGTGGAATGATA550.1030155459823937No Hit
ATTCAAGGGCCGCGCATTTCCTTGGCCTCATCTGAGATCTGGTGTCAGAGTT550.1030155459823937No Hit
GCCGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC550.1030155459823937No Hit
ACTTGAGGGCATAATCACTTGTTCCTTAAATAGGGACCCGTATGAATGGCTC540.1011425360554411No Hit
CTTTCAGGGTGTTACAACTAGTATATATCACTTTTAAAATACATAAGCGTGT540.1011425360554411No Hit
TCACATGGGGTATTCCCAGATTCTTTCCTGAATAGATTTGAATTTAATGTCA540.1011425360554411No Hit
CGCGCTGGGCTGGTGAGCTCTGAGTAAGGTTAGTGTCTTCTAACTGTCTTAC540.1011425360554411No Hit
TTATTAGGGAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGTGAAGCT540.1011425360554411No Hit
TGCTCAGGGGCCTTGCTTCCTGCTTCGCCTCCGCGCCTCGCGCTATGGGACA540.1011425360554411No Hit
CTGCAAGGGAAGAAAGGAAGCAACAGATAGATGTTGCTCTTTCACCTGGGTG540.1011425360554411No Hit
CTCCAGAATTCCCTAGATCCAAATTGCTCTTCTCCTTGGCCTTTTGTGCTTC540.1011425360554411No Hit
ATGTTTGGGATTGTGCCTCTCACTGGAAATAAGATGTCCTTCCAGAAAGAGG540.1011425360554411No Hit
TCAGCTGGGATAAGATCCCTGACTATAAAGTAAGACTTATTATCTTGTATAG540.1011425360554411No Hit
CCACATGGGCGATCTGCACGGGGGATTTTTTAAAACTCTTATCAAAGTATGA540.1011425360554411No Hit
CAAGTCGGGCTGGCCCACAACTCCACAGCCACACTCTCCGTCATCAGGGACT540.1011425360554411No Hit
TTGAAAGGGTAATGACCTTTTATATAAAATGGGTTATTTTAGTCCTTGCTTC540.1011425360554411No Hit
GTATCAGGGCAGCACAAGAGAGATGAAGTAATTTGCATATGATGGCACTATT540.1011425360554411No Hit
ATACTGGGGTGGGGAGAGGAGGACATTTGGGCTCACCTGTCAAGGTGGCCCT540.1011425360554411No Hit
TCTTAAGGGGAGCAGCATTTTTGTAACAAAGAGACTCGCTGGAGCTATTTGG540.1011425360554411No Hit
GTTGCTGGGTAAAGCTCCCCTTCTGCTGACAGCTTTCTTCTTAGCCAAAGCC540.1011425360554411No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)