FastQCFastQC Report
Sat 31 Dec 2022
SRR5849405.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849405.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54055
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3560.6585884747016927No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3160.5845897696790306No Hit
TACCACGGGGCAGTCTTGCCATTCTTAACTTATTGTGACAATAAAATTTAAT990.1831467949310887No Hit
CGCATTGGGGTTAAGAGACACATTTATAGCACTAAATGCCCACATCAAAAAA940.17389695680325595No Hit
CTAAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.16834705392655627No Hit
TGAAATGGGGCCGGCCAGTGGCGCTTCGTGGACGTGCTGGGGCTGGAAGAGG870.16094718342429007No Hit
ATTGGTGGGGATATCTAGGTAGCTTACTTTGTGTTTGTCATTCTTGTTTGTT860.15909721579872352No Hit
TCGGTCGGGAATGAAATTCCTGTTTGAGTCCCACAGTGAGTAATTCTTATAA860.15909721579872352No Hit
AGTAACGGGTGATTAGATAATCTGAAAACCTTTCTTCTTCAAATCACCTAGA850.15724724817315697No Hit
ATGCGGGCAGTGACACAAGTCTCTCAAAATGCATGTGGCAAATCTTTTTTTT830.15354731292202387No Hit
GTACATGGGACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC820.15169734529645731No Hit
ATTGCGGGGAAAGCAACCTGCATTGCCAGTGTGGGACCCTGGTCTCTGGTTC800.1479974100453242No Hit
AGTACAGGGCGGCAGTATTGTTTTCTTATATGTGCAATGAAGTGGAATGATA790.14614744241975766No Hit
CCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG790.14614744241975766No Hit
GTCTTGGGGAAAGTTTGGAAATGTTTTTCGTTTTTGTCATAAAAGAAACATG780.14429747479419108No Hit
TGTTCAGGGCACCTTGATCAGGTAAAGAAAAAAAATCTAAATCCAAAATTTC780.14429747479419108No Hit
AGATAAGGGGCATCTGGCCTGTTTCTTCAGAATAGATTTCTAATAATTACAT780.14429747479419108No Hit
GGTCCGGGGCTGTTCCCTGGATTAAAAGAAGCCTTTGTTGTTGAAGAATGGG770.14244750716862453No Hit
GTAATAGGGGATAAGTGTTTGCTTTTACTATTATACATAAAGTTATTGTCAT770.14244750716862453No Hit
CTTTCCGGGATGCCTAGAAAAATCTGCCTAGGCCTTTTACAAAAGCTGTCTT750.13874757191749146No Hit
CCTGCCTCAGCCTCCTGAATAGCTGGGATTACAGGCACCCGCCACCATGCCC740.13689760429192488No Hit
TCTAACGGGTATCTCAGGCAGATTTATCTCTTTAAAACAAGTGTGTAGTTCA730.13504763666635833No Hit
GGCATTTGGGTCAAGGTTTTATTAAAAGCTACATTTTATAACACTGGCACAC730.13504763666635833No Hit
AATAGTGGGATGAGAAGAGCAGAGGCCACTCTCCCAAGCTTCATCTTCTGCC730.13504763666635833No Hit
GTGGAAGGGATTTTACAGGTAAGTTTTTCCAGACTTTCTGGAAAGAGCAAAT720.13319766904079178No Hit
ATGACAGGGATGATTTGGCTCTGTGTTTTCCCTCAAGTCTCATGTTGAATTG720.13319766904079178No Hit
GCAGAAGGGCTGAGAAAATCAACAATTCCATAAAAGCAACAAGACCAAGCAA720.13319766904079178No Hit
CACATAGGGACTCCTCTGTCCGCTCCCTGGCCTCTTTTAATGGCTACCCTGT710.13134770141522523No Hit
TGCTCAGGGGCCTTGCTTCCTGCTTCGCCTCCGCGCCTCGCGCTATGGGACA700.1294977337896587No Hit
GCATTTCCTTTCCTCCAGGAATACACAGTGAAACTAAGTAATATCCCTACAA700.1294977337896587No Hit
AAACAGGGATCCCACAATGCAGAGACTTACCCACAAAGCAAATGATTACCTC700.1294977337896587No Hit
GCACGAGGGACTCAAAAGCAGGAATTTTTAAAGGAATCTTTCATACAATTGG700.1294977337896587No Hit
ACAGCTGGGGAATTAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGT690.12764776616409212No Hit
TTAACCGGGGAGGCAAGTGTGGGAAATGTGTGAACCCACGCTCATCCAGTCT680.12579779853852557No Hit
TAGCCAGGGAACACTCAAGGTTAAATTGCATGTTGTTATTAGAATCAATGTT670.12394783091295902No Hit
CTAATGGGGTGAAGCCAGCAATTGTTTCGCAATTCGGCATTTTGAAAACAAA670.12394783091295902No Hit
GAAAAGGGATTCTAGCAGCATCCATTCTTGTTTAAGCCTTCCACTGTTAGAG670.12394783091295902No Hit
TTTGCTCGGGGTCATTAAATAGTAAATTTAAATTCCTTCCATTTAGTTTGGT670.12394783091295902No Hit
TAGTGGGATCACACAAGATAAATTTAGATTTCAGTCAGTACATTTTATTTCA670.12394783091295902No Hit
TATGCGGGGATTTTAAAACTGAAACAATAACCAAATAACATTGGATAATAAA660.12209786328739247No Hit
AACACAGGGGGGGTTTGAAGCCGCGCCGCGAGGGAGCGAGGTCGCAGTGACA660.12209786328739247No Hit
CACCAAGGGATTAAAAGAAGCAATTATGAATAATGGAAAGTAAACAAATCTA660.12209786328739247No Hit
AATACAGGGGTTTTCCCTCAGGGTCCAGTATGCCTTTGAGCTTTAGCTGTTA660.12209786328739247No Hit
GTTGCAGGGGGCCCACAGTTCCGACGAAAAATGGCGGGGTCTCCTGAGTTGG650.12024789566182591No Hit
ACTCCAGGGAGATCCACATTCAAAAATTGCTTTGTATTTCTATATACTAGCA650.12024789566182591No Hit
CACCCAGGCTGGAGTGCAGTGGCATGATCTCGGCTCACTGCAAGTTCCGCAT650.12024789566182591No Hit
GTAAGAGGGAAGAATTGGAATTTGGAATTACTTTACCCACATTTAAAATGAA650.12024789566182591No Hit
TGAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC640.11839792803625937No Hit
CAATCGGGGATGATCATGCAAAATTAACCTTGCCTATTTCTGGGATGAAAAA640.11839792803625937No Hit
GGCCTTGGGATCTCTCCTTTCTCATCATTTTCACTAAATCAGCGTAATGACT640.11839792803625937No Hit
GTGGCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC640.11839792803625937No Hit
TGCTTAGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC640.11839792803625937No Hit
ATTCAAGGGCCGCGCATTTCCTTGGCCTCATCTGAGATCTGGTGTCAGAGTT640.11839792803625937No Hit
GGGTTGGGAACAGCACTCTACCATCGATCCTATGTGGAATGTCCGCCACCTT640.11839792803625937No Hit
TAAAAGGGGGAAAAACACAAGACAGAATTGGAAAGCCTTAAGCATCAGCAGG640.11839792803625937No Hit
GTCTTTGGGACTGTTCCTCTATAAACTCAACTTGAATAATGGTTATTCCTTC630.11654796041069282No Hit
GCGTAGGGGAAGGCCCATCAGCGACAGCTGACCCCGCCTGAGGGCTCCTCAA630.11654796041069282No Hit
TACGTTGGGAGCCAGGCAGAAGCCTTATTAATGAAAGCTGGAGTGCAGCCAG620.11469799278512627No Hit
GTATTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC620.11469799278512627No Hit
TTCTGGGGGATGGATGCACAGGAAGCTTTAGCTAGAAGGTTGGAAAGGGCAA620.11469799278512627No Hit
TGCGACGGGATAATAGCTCTGCTGCAGTTCTGTGACTTCCAAGCCAGGAATT620.11469799278512627No Hit
AGGTGGGAGACATGGCACATGGATGTGATCTTCAAAAACTGTTACAGCCTCA620.11469799278512627No Hit
GATTAGGGGCCCGTATGTTCTGAGTCTTGCTGTTTTTACCTGGTAATATTTA620.11469799278512627No Hit
TCGTTGGGGTCGGGACCGTGGGCCTTCCCTCCATTTCCCACAAGCGGTATGC610.1128480251595597No Hit
TCGGTCGGGGGCTAACTTACATACAAATTAAACCTAGTATTATTCATCACTG610.1128480251595597No Hit
ATTCTTGGGCGGCGACAGAGGACAGCCAGAAGCCCAGCCAGCCCCAGACTCC610.1128480251595597No Hit
GTGACCGGGTTTCTTAGTATATGAAAGGACAGGGATTTTTGTTCTTGTTGTT610.1128480251595597No Hit
CGCATAGGGAACCAAGTAAATTTTATTACTGAAATGCAAGGATGGTTCAGCA610.1128480251595597No Hit
TTCCTGGGGAGAGACCGAGGGGAGAGAGGTCGATATAGAAGATAATGGGCTT610.1128480251595597No Hit
AAAAGCGGGCGATCTAGGCATGGGAACTTTCAAAAGCTTCTGAGTGTCCAAT610.1128480251595597No Hit
ACTACTGGGGGCATTATTTCTAGCACATTACAACCAATCTTCATTCTCTATC610.1128480251595597No Hit
GAACATGGGATGTTTATTTAATCCATAACAGAAATGAGTAAGGGTAGCACAA610.1128480251595597No Hit
CACCGCGGGACGGCGGCCGGGCGGGGACACGGTTCGGGGGCTCCGGGCTGGG610.1128480251595597No Hit
GCTTAAGGGATTTTAGTTTAGGGGTTAAAATTCAATTTGGGAACTGATAAGG600.11099805753399315No Hit
ATAAACGGGGGATTTTCTGGTTCAAAATTTTACTGGCTTTCAGAAATTCTAA600.11099805753399315No Hit
TATTGCGGGATGGAGTCACTGATTCATCACTTTAAGTTGTATACTGAGGGCT600.11099805753399315No Hit
TTGCTAGGGGTTGGAAATGTATTTGAAAAAATTCTCACTTCTGCAAGGCCAG600.11099805753399315No Hit
GTAGTCCAGGGGTGGGCATCATCTTATAATCAACTCCCTGTAAGTCTCAGAT600.11099805753399315No Hit
GCTATCGGGCCGAGATGCTGCCCGCTACCACTTCTTCCTCTACAAGCACACC600.11099805753399315No Hit
GTCATTGGGGATTTGTATTTTAATCTCTCACCTCTTTTTCTTGTATTTTCAT600.11099805753399315No Hit
TTGATGGGGTACTGAATTCCTTTTTTAAATAATCTTTTGAAACTAAGAAAAT590.10914808990842659No Hit
TTGTCAGGGACTTACTTTCGGTCCAGCATTATGCTTTACATACTTAAATTTC590.10914808990842659No Hit
AAACGCGGGTACTTGAGGCCGGGAGTTTGAGACCGGCTTGGGCAATGTGGTG590.10914808990842659No Hit
GTTCATGGGGAGCTTGCTGGCATGGTGGGCAGTATTCCAGGAGAGGCCATGT590.10914808990842659No Hit
GACTTCGGGCTTCCCAGGAATAGTACTTAATAGATTTTAATGTTAATTTATA590.10914808990842659No Hit
GTCAATGGGCTCCTTCCCGGCGGCAGCTACCGAGAGTGCGGAGCGACCAGCG590.10914808990842659No Hit
CGTTCTGGGAGGCATGTGTAGAAGGCAATTTATCAAACCTATTGCACTGCCA590.10914808990842659No Hit
TAGAGGCGGGGAGAAGAATTTAGTTATTACCTAATATTTAAAACTTCTAAGC590.10914808990842659No Hit
ACATACGGGTGCATTAAAAGTCTTCATCTAAACTGTTTGCAAAAGGATAGTG590.10914808990842659No Hit
CAACATGGGGTGGAAAACACGGATGGGAATGGGGCTTATCCTGTACCAAGGT590.10914808990842659No Hit
AACATCGGGGATTACATGTTTTTACAGTGGCCTGCTATTGAGGAAAGGTATT590.10914808990842659No Hit
TGCCAAGGGGAGCCTGTCAGGGTGGTGGTCCTAGACTTCAGTGGTGTCACCT590.10914808990842659No Hit
TGAGAGGGGGCTCATTGCAACCTCCACCTCCTGGGTTCAAGTGATTCTCCTG590.10914808990842659No Hit
GTTAAGGGGATCCCATGCTTCCTCCAACAATTCCAGTGAGGAGTTGACATGG590.10914808990842659No Hit
TCAGGTGGGAGGTACCAGAGTCAAAGGATGGTACAATTTGATAAAATCATTT590.10914808990842659No Hit
TCAAAGGGGGTACTATAAAGCTACAGTAACCAAAACAGCATGATACAGGTAG590.10914808990842659No Hit
CTTTGCGGGAGAACTTAAAGTATGATAATACGAAAAACAAGATAGGTATATA590.10914808990842659No Hit
ATGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.10729812228286006No Hit
GGTACAGGGCATGAACGACTGAATTAGAAATGGAAATGCCTGGCACAGCCAG580.10729812228286006No Hit
CACGTAGGGGGGCTGCTGCGGCCCAGCCCCGAGCCTGCCTCCCTCTTGGCCT580.10729812228286006No Hit
CACCTTGGGACATGAAGAATTACAGCAAGACATGCAAAAGTTTAAGAATGAG580.10729812228286006No Hit
ATAAATGGGTCCTGTTAAGAGAAAACAGGTGTTTAAAATTGATTTTTAAAAT580.10729812228286006No Hit
CTCGCAGGGTATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCC580.10729812228286006No Hit
GGCAAAGGGTAAGGAGAACTAAACTACAACCCATGCTGGTATTCCCAAGAGA580.10729812228286006No Hit
ACTAGAGGGGAGCCTCCAAAGTGTCCCATGCTAGGGGAACTTCACGTGGGGC580.10729812228286006No Hit
TTGGCTGGGATTTAAATTTACTTTGAAATATATACATACATATGAATGAAAG570.1054481546572935No Hit
GAAGCAGGGTCAACACTGGAGAATTGAAAAATATGTATTTACAATCCCTAGA570.1054481546572935No Hit
TCAAACGGGACACTATGAGAAAATGTTAAGATACATCAAGGATAAGAAAAGA570.1054481546572935No Hit
TGCCAAGGGAGAAAATAGCAACAACAAAAATAATATGAGATGGTTTTCAAAA570.1054481546572935No Hit
AAGCCTGGGAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGC570.1054481546572935No Hit
TTGTTAGGGGGCACTGGTCATTTTTTAAAAGCCCCCAGTTGATTCTAATGTG570.1054481546572935No Hit
TGTCAGGGGAACCCTCCCCACCTTGTTTTCCACTGGGAATGAGAATTTGATA570.1054481546572935No Hit
AAGTGTGGGTGTCTCAAAACCCTCCTCATCATCTACTCCTTCGTCTTCTGGA570.1054481546572935No Hit
AGTGCTGGGATTACAGGCGTGAGCCACCACACTCAGCCTACGAACTTCTTTA560.10359818703172695No Hit
TACGGTGGGAGGCAGAGAACTGAAGATACATGTATATCTCAAACTTGTGAGT560.10359818703172695No Hit
AATAGTGGGCATTTACTCTGGACTGGTGAAAATATTTTTTAAAGCCAGTGCT560.10359818703172695No Hit
AAGGAAGGGTGTGGCCTAATAGTGTCTCCCTCAGATGCCTCGTGGTTGGTTT560.10359818703172695No Hit
GCATGAGGGAATAAATGCTTAGATATTACATAATTCAGAGAAATGTACAGAG560.10359818703172695No Hit
GGGACCTTGCACCGGGACTGAATTAATGTATTCAATGGATCATTTGAACTTA560.10359818703172695No Hit
TGGCATGGGAGAAGCCTAGCTGTTATAGCAGCAAGCTTGCAACCAAAAAACT560.10359818703172695No Hit
ACTGTTGGGGAGTGTAATCAAGAAGAGTATTCTGTACTTGACTCTTCTTATC560.10359818703172695No Hit
TGAAACGGGCATGTGTCAATTTTTAGGTCAAGCTGTTTCTGTTACTTCACTT560.10359818703172695No Hit
AGCAGTGGGTAGGAGTGAGAGGGAGGAGGAAAGGATGTAGACACATAATTCA560.10359818703172695No Hit
ATGCTGGGGTATCAAAGGAGAATTTTTAAAGCCTTAACAATGCCTACTTTCC560.10359818703172695No Hit
CTCAGTGGGCTGTGGGGATGGGAGGCATTATTATGAGAACACTCACTTTTCT560.10359818703172695No Hit
CGTTACGGGGTTTCATGGGGTTAGGCTCTGCCAGCCTGTTGGCCAAAGTACT560.10359818703172695No Hit
GATCCTGGGCGATCTAACAATGGGGTTGCCCCAGAAAGGGATGTTATTATCC560.10359818703172695No Hit
ATAAGTGGGCTAGAAAAGACATGAAGCTATGTTGAGGCCTGATAAGACCTTG560.10359818703172695No Hit
ATATATGGGGTCCCCTCTAAGACTTTAATTTTGTGTTTACTCTCTTATTTTT550.1017482194061604No Hit
TTAAGTGGGTGGCAGCATATTGGACACTTTTTGTTTTTTGCTCCTTCTGTTC550.1017482194061604No Hit
GCTCTTGGGCATGCATGATTAATTTTAGAGATTTAGTCCTTAATGTTAAAGT550.1017482194061604No Hit
CATCATGGGGTGGGTAAAGGGCACAGAGTGAGACAAATGAGAATTATACCAA550.1017482194061604No Hit
ATAAAGGGGCCCTTGGATTATGTGGTTTCACCTCAAATCAAAGAAGAACTGT550.1017482194061604No Hit
ATGGTGGGCACAAAAGCTTGATGCAGTTTTTTGACATAGCAGACTAAAGTGG550.1017482194061604No Hit
ATAACAGGGATACCAATTGTTTATTATGTGCAAGTTACACTTCATCAAAGAA550.1017482194061604No Hit
GGTACTGGGATTTGTTAAAAGCCATTGTATGACTGAAACAATCTTAGTGCCG550.1017482194061604No Hit
CTCGAAGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG550.1017482194061604No Hit
GGCGCGGGCAACAAGAAGTGTTCAAAATATTTTAAATATTTGTGCATTTTAT550.1017482194061604No Hit
GGCTATGGGATTCATTTATACACCATCAGCAGACATTGGCATCTTCAAAATT550.1017482194061604No Hit
AGAAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC550.1017482194061604No Hit
TATCTGGGGAGGAAGGTAGTGACGTCATAGGTAAACAGGCTCTGTATCCGTG550.1017482194061604No Hit
GAGCTGGGGAGGCGCTCCGGGAACAGTGCATGTGCATGCATACCACAAGACA550.1017482194061604No Hit
CTAGTTTAGGGACAGAAATTAACTTGGAGAGCCTGAGAAATTCCCAGTGGCC550.1017482194061604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)