FastQCFastQC Report
Sat 31 Dec 2022
SRR5849391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81311
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3540.43536544870927674No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3520.43290575690865934No Hit
TCAACTGGGAGAAAACAAGCCCTCTCTCTTGTGAAGTATCTTCAAAGGATTG1080.13282335723333866No Hit
TTGCTGGGGTATGCATCTCTGGGCTCTGAGGACAAAATGCCTTGAGAGTAAA920.1131458228283996No Hit
ACACTAGGGTACTGGGGAGGCTGAGGCAGAAAAATCTCTTGAACCCTGGAGG910.11191597692809091No Hit
AGCATAGGGACCGTGATCTTCGCCATCTTCTCCGCTATCATCTTCCTGTCAT910.11191597692809091No Hit
TAGCGTGGGGGCTCTAGGGTGAGCTCTGCCCGGCTGCAGGGATGGCGGGGAG840.10330705562593007No Hit
ATCGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC830.10207720972562138No Hit
ATGTGCGGGTCTATATTTTAGTAAATAGTTCTGCCATGCAGTCAGATGATCT830.10207720972562138No Hit
TATACAGGGGCCCTAATTTAGATTTGAGGAAACTGAAGCTGAGAGAGGGTTA830.10207720972562138No Hit
GGCCTGGGGAAGCTGATGCAAGAAAACCACCATTGTAGCCACAAAATCAAAA820.1008473638253127No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)