FastQCFastQC Report
Sat 31 Dec 2022
SRR5849383.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849383.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75670
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4680.6184749570503502No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3690.48764371613585306No Hit
GAGCACGGGCGACAACCCTCAGTCAGACAAGGAGGCCCTGCAGGAGGGCCGC1200.15858332232060263No Hit
TAGCTGGGGATAGCACCAAATGCCCATATCAAAAAGCTAGGAAGATGTCAAG970.12818818554248712No Hit
CACATGGGGAGAGAACATGGCTCATTCACTGGTCTCTGTTTGTTTTCTTCAA940.12422360248447205No Hit
TCCCACGGGTCAGCTCACTGCATCCTCTGCCTCCCGGGTTCAAGCAATTCTC900.11893749174045196No Hit
ATCTAGGGGGGCCATCTCTCAGGAAAACAGAGCTGTTGTATCATAAAGCTTT900.11893749174045196No Hit
TGCCAACGGGAAGTGCCCATTAGGGCAAGAGGGCGGCTCAGAGGAATCAGGT900.11893749174045196No Hit
TTTTCCGGGACATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGACAC880.11629443636844192No Hit
TGACCATGGGGTCTCCTGAGAAAGAAGAAAAAGTGATCACTGTGCAATATTA870.1149729086824369No Hit
CGATATGGGGTGCCTGAGACCAAATTTTCTTTTAGATAAATGTACAGGATTC860.11365138099643188No Hit
ACCGTCGGGACTGAGGAGCACAAAAAACTTGAGCAAATGAAAACCACATTTA860.11365138099643188No Hit
GGACCGGATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGA850.11232985331042686No Hit
GGGCCTGGGGAAGTGACAGGGAGGGCCGGAGTCAGCAGGTGCCAGACTTTCT850.11232985331042686No Hit
GTCAATGGGGTCTGACAGAGTTATTTTGGAGAAGCAGTCTTCAGGTTCTGAG840.11100832562442184No Hit
TGATTGGGGACAAAAATCCAGTTTCTCTTGATGAAGAAGAAGATGGTGGTGG840.11100832562442184No Hit
AATCTTGGGTTCTGAGTAGCTGGGACTACAGGCACCTGCCGCCACGCCTGGC830.1096867979384168No Hit
TGGCTAGGGGGCCGAGAGTCGGAGCCATTACTGCAGGAAAAGGTCCCGGAGA830.1096867979384168No Hit
CAGGTCGGGAACAAAAGGAGTAAATGGATTTAGAAGAATTTCCTAAATGAGA830.1096867979384168No Hit
CACCTGGCTACTTTTTGTATTTTTGTTAGAGATGGGATTTAGCTGTGTTGGC810.10704374256640677No Hit
TTAACGGGAAAGGCAAAAAACTAATGAAGAAACTCTGGATGTAAACTTGACA810.10704374256640677No Hit
GACCAAGGGTTCTCAAGTCTAAGAAGTCAAGCCAGGGATTAACCTTCAGCCT800.10572221488040175No Hit
TAACAGGGAATGTAGGAATGAGAGAGACAGGGAAGAAAAGACTGAAGGAGAA800.10572221488040175No Hit
TTTACACGGGCCCCTCCTCCTTTCCTCCCTCTACTCTGACACAGCACTTAGC800.10572221488040175No Hit
GTGTCCGGGCCATATTTGGTAAGATGTAAAAATCCACACATATAAGAAGCTC790.10440068719439673No Hit
GTCAGCGGGCAGTGTTTGAATCATATGCTGATAACTGTTTGCCTGTGACCCT790.10440068719439673No Hit
GCGCGACGGGCTCATGGTGATTCTTGATGAGCAGAAGTTGATAACTTTGGTG780.1030791595083917No Hit
TTCACCAGGGAACGAAAGTCCATCACTCGGCACGGCTCACTCTCCTCCCGCA780.1030791595083917No Hit
ATTACCGGGGAAGCGCTGATCCTGTAGTCACCTATTTTCGTACTGAAACATC780.1030791595083917No Hit
AAATGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC780.1030791595083917No Hit
TTGCCAGGGATTTCATTCTTTTTCGTGGTAATTGTGAATGGGATTGCATTCT770.10175763182238669No Hit
AACAATGGACTTTAAAGGAACTTTCATTTCAAGAGGGCTGAAAATCACTGCC770.10175763182238669No Hit
CGATTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.10043610413638165No Hit
AGGAACGGGGGGGGCTTCCCATGGGAATTGGGAGGTCCCAAGGCAGAGTCAG760.10043610413638165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)