FastQCFastQC Report
Sat 31 Dec 2022
SRR5849382.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849382.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75358
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4710.6250165874890522No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3920.5201836566787866No Hit
GAGCACGGGCGACAACCCTCAGTCAGACAAGGAGGCCCTGCAGGAGGGCCGC1120.14862390190822475No Hit
TAGCTGGGGATAGCACCAAATGCCCATATCAAAAAGCTAGGAAGATGTCAAG1080.14331590541150244No Hit
GGGCCTGGGGAAGTGACAGGGAGGGCCGGAGTCAGCAGGTGCCAGACTTTCT1040.1380079089147801No Hit
CCTCGGGGGAAATGGGTGACCTGAAGTGACACTTTTTCCACAATTAACCCAA1030.13668090979059955No Hit
TTTGTTGGGATGCTGTAAAATGAATGAAGACACCAAAAAAGGGCTTTGAAAA900.11942992117625204No Hit
GTTAAGGGGAAGACAGCAAGAGGGGAAGAAAGAAACAAAGGAATGACAAAAC900.11942992117625204No Hit
GTCAACGGGAGACTAATTGTGTTTGTCCGTCCATGCCATGAATGAGTGGGCT890.11810292205207144No Hit
CAAACAGGGACTTGCCTCACACCCACCTCTACATCTTGATACTGTATTTTGC890.11810292205207144No Hit
TTAACGGGAAAGGCAAAAAACTAATGAAGAAACTCTGGATGTAAACTTGACA860.11412192467952971No Hit
GTCAGCGGGCAGTGTTTGAATCATATGCTGATAACTGTTTGCCTGTGACCCT860.11412192467952971No Hit
TTCGCTGGGATGAACGTGACTGGTACATTGGTTTGGTATCTGATGAAAAGTG840.11146792643116855No Hit
TCGTAGGGGAGTGTATATTTAATGTTGATTGTTGAATTTTAGTTACGAGAGG820.1088139281828074No Hit
ATTAAGGGCTACCATGCATCATAATTATGCTAACCGCTAAAATGAAGCTTTG820.1088139281828074No Hit
TAAATTGGGTATTATGGGCAATATGTAAATAAATAAATGTAATATTTCATCC820.1088139281828074No Hit
GCGATGGGAGTGACCAAGGGACTGACAAATGTCTGCTTTGAGGAGTAGCAGA820.1088139281828074No Hit
GTAATTGGGCTGGCGATGGGCGACGTTCTTCCGAGCTGATCTAGGGAAGGTT820.1088139281828074No Hit
TTTTCCGGGACATAAAAGCCTACTTGAGAAGAGCTCAGTGTTACATGGACAC820.1088139281828074No Hit
AAGATGGGGAGTCCAGGAAATTCGAGAGCATCTTAGGGCCAAGGAGAGTACA810.10748692905862682No Hit
GCGCACGGGATCTTAGACAGGGAAGCAAGAGCTAGACACACTTCAGAATATA810.10748692905862682No Hit
AATGAAGGGGGGAGTCACGGCTCGCGACTGGCCTAAGTCGCCGCAGAACTGC800.10615992993444626No Hit
AAGCTTGGGCGTGGGCGTTTGAAGTGAAGTGATGTTGCAAGGCGACTTGCAA790.10483293081026566No Hit
CTTTGGCGGGAGGAAAATGTGACTGTAAGTAGAGTGACCTTTATATTATACA780.10350593168608507No Hit
TAACAGGGAATGTAGGAATGAGAGAGACAGGGAAGAAAAGACTGAAGGAGAA780.10350593168608507No Hit
TGACCATGGGGTCTCCTGAGAAAGAAGAAAAAGTGATCACTGTGCAATATTA770.10217893256190451No Hit
AATAGTGGGACTTCGTCTTCACGGCGCGGACGCGGCTGGAGAGGCTTGTGGA770.10217893256190451No Hit
GGCTAGGGAAGGTTTTACCCTTGATACGGAATTTACACAGGTAGGGAGTGTT770.10217893256190451No Hit
CACATGGGGAGAGAACATGGCTCATTCACTGGTCTCTGTTTGTTTTCTTCAA770.10217893256190451No Hit
TATCAAGGTTAAGAATCATGGGCATCAAATATTGATTTAGAGAGGTTTTCCC760.10085193343772393No Hit
CAAAGTGGGCTGCTCTGTCAAAATCTGCCACTCTCTCTGGTTACTGTGATCC760.10085193343772393No Hit
CCGTCTGGGCGATCTACGCGTGGGATAGAGAGATGAGGTTCTCTTTTTATAC760.10085193343772393No Hit
AATGCAGGGGAGTTATTTGCTATTATCTTGCTTTCCTGCTGTCTTTGGCAAA760.10085193343772393No Hit
GTCTGCGGGGCGCAGTGGAAGCTGCTGGACGAGGACGAGAAGCGGCCCTTCG760.10085193343772393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)