FastQCFastQC Report
Sat 31 Dec 2022
SRR5849381.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849381.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75325
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT5120.679721208098241No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3540.46996349153667444No Hit
GAGCACGGGCGACAACCCTCAGTCAGACAAGGAGGCCCTGCAGGAGGGCCGC1270.16860272153999337No Hit
TTCACCAGGGAACGAAAGTCCATCACTCGGCACGGCTCACTCTCCTCCCGCA1030.13674078991038832No Hit
TTAACGGGAAAGGCAAAAAACTAATGAAGAAACTCTGGATGTAAACTTGACA930.12346498506471955No Hit
GTTAAGGGGAAGACAGCAAGAGGGGAAGAAAGAAACAAAGGAATGACAAAAC880.11682708264188517No Hit
TAGCTGGGGATAGCACCAAATGCCCATATCAAAAAGCTAGGAAGATGTCAAG880.11682708264188517No Hit
ATTAAGGGCTACCATGCATCATAATTATGCTAACCGCTAAAATGAAGCTTTG860.1141719216727514No Hit
GGGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.11284434118818452No Hit
TTACCTGGGAGAATCCCTTTGATTTTTTTCAAAGAAAGAATACTGAAAAGTG850.11284434118818452No Hit
ATCTAGGGGGGCCATCTCTCAGGAAAACAGAGCTGTTGTATCATAAAGCTTT840.11151676070361766No Hit
CAAACAGGGACTTGCCTCACACCCACCTCTACATCTTGATACTGTATTTTGC830.11018918021905079No Hit
GGCTAGGGAAGGTTTTACCCTTGATACGGAATTTACACAGGTAGGGAGTGTT830.11018918021905079No Hit
GGACCGGATGGATGAATGAACAGTGTAGCAGTATCATCACAGGAGGGGAAGA820.10886159973448391No Hit
CATCCGGGGATACTGATTTCACTTTCCAGAAATGGAGATATGAAAAGATTCT820.10886159973448391No Hit
GGGCCTGGGGAAGTGACAGGGAGGGCCGGAGTCAGCAGGTGCCAGACTTTCT810.10753401924991701No Hit
GGTCCTGGGAGGAGGTCAAAGTGGGCTTGGTGGCTAGACGCCAGGCACCAGC810.10753401924991701No Hit
TAAAGAGGGAAAGATAAAAATTCACAGTAACTTGACAACAAAACTTATATTT800.10620643876535014No Hit
CTTTGGCGGGAGGAAAATGTGACTGTAAGTAGAGTGACCTTTATATTATACA800.10620643876535014No Hit
AACTAAGGGCCCGGGGGCGGTGGAGCCTCATGATCTCTTATCTTTTGAGGCT790.10487885828078328No Hit
GCGCACGGGATCTTAGACAGGGAAGCAAGAGCTAGACACACTTCAGAATATA790.10487885828078328No Hit
AACAATGGACTTTAAAGGAACTTTCATTTCAAGAGGGCTGAAAATCACTGCC790.10487885828078328No Hit
GCTATGGGGGAAACTTTAATGTGAGATTAAATTTTCAAATTTCACAAATTAG790.10487885828078328No Hit
TAACAGGGAATGTAGGAATGAGAGAGACAGGGAAGAAAAGACTGAAGGAGAA790.10487885828078328No Hit
TTTGTTGGGATGCTGTAAAATGAATGAAGACACCAAAAAAGGGCTTTGAAAA780.1035512777962164No Hit
TGCCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAG780.1035512777962164No Hit
CAGGTCGGGAACAAAAGGAGTAAATGGATTTAGAAGAATTTCCTAAATGAGA780.1035512777962164No Hit
CGATATGGGGTGCCTGAGACCAAATTTTCTTTTAGATAAATGTACAGGATTC780.1035512777962164No Hit
CGAGCTGGGCCATCTAAGGTTCTTTGTAGATGCATGTGTAAGAAGCTATTTT770.10222369731164951No Hit
GTGTCCGGGCCATATTTGGTAAGATGTAAAAATCCACACATATAAGAAGCTC770.10222369731164951No Hit
AAATGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC770.10222369731164951No Hit
GTCAGCGGGCAGTGTTTGAATCATATGCTGATAACTGTTTGCCTGTGACCCT770.10222369731164951No Hit
TTGGTGGGATATGCACCTTTCAGAAATTTGTGTTCGAGTAAGTAAAGCATAT760.10089611682708263No Hit
TCGTACGGGGCAGATGTGGATCAGCAAGCAGGAGTATGACGAGTCCGGCCCC760.10089611682708263No Hit
AAACAAGGGATAATGTTTTATAATTAAAAATTTCTCTTTATTTTGTTCTATC760.10089611682708263No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)