FastQCFastQC Report
Sat 31 Dec 2022
SRR5849380.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849380.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63822
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4010.6283099871517659No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3150.4935602143461502No Hit
CAGCATGGGCAAGTACTGAAACTCTATACCCATTAAATAACAACTCACTATT1060.16608692927203786No Hit
ATGGCGGATGGCATAATTTGGAAATAATGCTTTCAACTTCCTTCTACTTCAT1060.16608692927203786No Hit
CACATAGGACGATAAAGTGATAATTTCTTCATCTCTCCATCTAAGGTTCTTT990.15511892450879006No Hit
AGGCCGGGAGAAAACTGGAGTAAGAAGTGCAATATCAGCCTAAAACAAGCAG980.15355206668546895No Hit
AAAACAGGGTGTGACAGTCAGAAAACACACCTGGAATACGATGCAGCCCACC970.15198520886214786No Hit
TCTGGTGGGACATGCTGGAAAAACAGTGCCGATTGTGAAAGGAGGGGAGGCA950.14885149321550564No Hit
GTTCCCGGCTACGAGGCCTAGACCAGCAGCTGGATGATCTACAGACCAAGTT950.14885149321550564No Hit
TAACCAGGGTTGAAGCAGCTGGCTGAGAAACGAGAGCACGAGAAAGAAGTGC950.14885149321550564No Hit
TAATCGCGGGAATTCCTAAGAGCCACTGACATCCAAAAAATTCATTACTTAC940.1472846353921845No Hit
AAATCCGGGATGCCCAGGGTTGTATTAAAGAATTTTTCTGCAAGACATTTTT920.1441509197455423No Hit
GAAAGCGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC920.1441509197455423No Hit
CATCCAGGGACTTTATGGCATATAAAAATACTAATTCAAGGTGAATCACAGA910.14258406192222117No Hit
TCGACCGGGATTTGCAGTTCCTGAAGACTTCAGAGTTTTATCCCAAAGAAAT900.14101720409890006No Hit
AGTCCAGGGCAGTCCATTTATGGAGACAAATTTGAAGATGAAAATTTTGATG900.14101720409890006No Hit
TTATGCGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC900.14101720409890006No Hit
CTGCCTGGGATTGTTTTACTGTAATTAGTGTGTACTTATATTTTATGTCCTT890.13945034627557895No Hit
TAATGTGGGATGACACCAGAGGTCTACAGGAAGGCGTGGAGCTGCCAAAGCA890.13945034627557895No Hit
ACTCAAGGGGACGGCCTCGCCTCGGTATCGCAGCGGGTCCTCTCTATCTAGC890.13945034627557895No Hit
GACAGCGGGTAAGAGGAATCCCTGAGAAAATGTAGATAAATTTAATCATATA880.13788348845225784No Hit
TGATCCGGGGAAAAAAATGAATGCCATTTTCAAGTTCCCAAATTACTTCTAT860.13474977280561562No Hit
AGTTTAGGGTAAAGCAGTTCGTAAGAAGGCAACAAGTTCTTCTCCTCTACAG860.13474977280561562No Hit
GTAGGTGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT850.1331829149822945No Hit
CTCTAGGGGCAACAGCAGTGATGAAGAGGAGGAGGAAGAGGAAGAAGAGGAA850.1331829149822945No Hit
AACCTTGGGCAAATTTCAGCTCTTAGGAGCTGCTCTACAATTCTGAATTTGC850.1331829149822945No Hit
GGCGCGGGGTGAAGGAATTATGGATGTTTTTATTTTAATTCTACTTTGCTGT840.1316160571589734No Hit
TTTAAAGGGGTACACATTTGTTTCAAGTGCAGATGGAGCATTTACCAAAATT840.1316160571589734No Hit
GCGCTCGGGTATGTTCTTTCACAGATGGGGAGAAAGTTGCAGCCAGTCTAAG830.1300491993356523No Hit
GAACTGCGGACTTTGGATCAGATGTGCAGTCGGAGAAGGGAAAATGACATTG830.1300491993356523No Hit
ATTAAAGGGAGCACCAATGGCAGCGGTGGCAGTGGCAGCAGTGGCCCAAAGG830.1300491993356523No Hit
TTGGATGGGGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAA830.1300491993356523No Hit
AATGAAGGGAGAGAGCTTACTTGAAGAAATAATGGTCAAAAATTTCCCAAGT820.12848234151233118No Hit
TAGTATGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAG820.12848234151233118No Hit
TATAACGGGAACCACATTTATTTAAGAATGTATTCCAATATCAAACAGCAAA820.12848234151233118No Hit
TCATGGAGGGCTATTATTGCCATGGAGATTTTAAGAGATGATGCTATATGTG810.12691548368901007No Hit
AGTAAAGGGAAGAAACTGTTCGAAAAAGAACAAAAACACTTCCCTCGTTATT810.12691548368901007No Hit
TTAACTCGGGGGCTGCGGTGGCACCAGCCAGGAGGCGGAGTGGAAGTGGCCG810.12691548368901007No Hit
CTCCTTGGGGAGATACGGACTTGATGGTGCTGTTTAATCAGTTTGCTTCCAA810.12691548368901007No Hit
TAAGTGGGGCATATCACATAAATAGACTCAAACAATATATGGCTTTTTTTTT810.12691548368901007No Hit
GTACAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG810.12691548368901007No Hit
CGTACGGGGCACAGTGGAAGGTCTGGATGGAGAGCGGCCACTGCAGACTGGG800.12534862586568896No Hit
GCATAAGGATTCCTACTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACG800.12534862586568896No Hit
AAACAAGGGAGGAGTTTTGGGCAGTGACATTTTTCACAGAATGTATATCTCA800.12534862586568896No Hit
AATGTTGGGGCTGTTTTTTATTCTGCCAAGGGCAGATTCATAATCACCGACA790.12378176804236783No Hit
CGCTTTGGGTATGGCGGGAACTGTCCTGTTGCTTACCAAATCCTGCAGGGCA790.12378176804236783No Hit
CCAAATGGGTTCATAGCGGGGATGGTGCTACATATATCTATGCAACTGAAAA790.12378176804236783No Hit
TACTCCACGTTTAAAGTATTAAATTACACTGAATAAACATTTAGGTAATGGG790.12378176804236783No Hit
TCACTTGGGGAGCATGCTGTTACTATTCTTGTTTTCTAGAAATTTTAATAAG790.12378176804236783No Hit
AATCACGGGGACTTGATTAAGAATTATTATGGAGAAAAAATTGGTATCTATT790.12378176804236783No Hit
TACCTAGGGGTTCTGATGTACTAAGTGACTATGGCAGATACCGATACTATGT790.12378176804236783No Hit
TAGTCCGGGATTGTCAATACAATTATTTTTGGAGTCTTTTAAACAGGAAAAT780.12221491021904674No Hit
TTCTCTGGGACCCGGAGGAGCACAGCAGATGGAGGGACAGATCCAGGACGAG780.12221491021904674No Hit
TAGCCAGGGTTACACCATTTGCCCACTTCTATGAATTTTATGTATAAAATTT780.12221491021904674No Hit
CTAGCAGGCTGTCAAATGTACCAACATTTTCGAAAGAATTCATTCAAATCTC780.12221491021904674No Hit
GCCACCGGGACTAAAAGACCAAGGGAGAGTCAAAGAAATGGAGGTGTAGAAG780.12221491021904674No Hit
AAAACCGGGGACCACGATGTTACATCCGCAAAGGATGGTGATTACTGGAGAT780.12221491021904674No Hit
GCTTAAGGGGGTTTTAAGGATCAAATCTATGAGATTTTCCAAAAACTAAACA770.12064805239572561No Hit
ATAACGGGGATCTAAAGCAGGGATATAAATCTGTAATAGATTTTAGAATTGA770.12064805239572561No Hit
CAGACTGGGGTCTGTTATGATCAAAGTGGATGGATCTTTTCCACAAGATAAC770.12064805239572561No Hit
TCTTAGGGGGAACTAAACTGCTATGATAAAAAATGGGGAATAGAGGAAATAA760.1190811945724045No Hit
AAACACTGGGAAAAGAATATGCAGAAGTTTTTGACTTGGGAAACAGGGTTAC760.1190811945724045No Hit
TCATAGGGGGCTCCCGGCGCGGCAGCTGTCTGGGCTGCTGCGCGCCGCCTAG750.11751433674908338No Hit
CAGTCAGGGAATGAGGATGTAAGCCCGTGGGCGATTATGAGAATGACTGCGC750.11751433674908338No Hit
GCATGTGGATAGCAATGTGTATGCACATTCTCTTTAGTTAAGGCACCAATTG750.11751433674908338No Hit
TCGAACGGGCGATCTGGTAACGGGTGCACGAGGGAGTCAGTGTTGTCCAAGT750.11751433674908338No Hit
CAACAGGGGTAAGTAGATTATTTTACAAAGAAATTTGAACAAATTTAATTGA750.11751433674908338No Hit
ACATAAGGGAGATCAAGGCATCAGCTTCCAAGTCTCTGCTTCCAAGGTAGTA740.11594747892576228No Hit
TTGCCTGGGCTCCTGCACCGACGACGTGTTTGCCTGCTTTGCCACTCCGGCC740.11594747892576228No Hit
TAACATAGGGACAATAAAAGATTGTAGGCTATCTCTGTAATCATGCAATAGC740.11594747892576228No Hit
GTCTCTGGGACTAGCTGTCAGCTTAAACCCTCTGTAATAGAACATGGAAACA740.11594747892576228No Hit
AATTGCGGGACCCGCGCCCGGGACGCGCATTTTCCTTCTCTCGCCACGAGTT740.11594747892576228No Hit
ATTGTAGGGTCTGCTTTTTGTAAAGCGTAAAAACATCACAAAGTAGGTCATT740.11594747892576228No Hit
CAAGTTGGGGCCTCTGTACATGAAATATGGGCTCCTGATGGACCTCATTCAA730.11438062110244117No Hit
GAACTGGGGTTGTCTTTCCAAAGCGCTGAGGCTGTGCACCTATTCTGTAGTT730.11438062110244117No Hit
CAATATGGGGGAGTTACAACTTGCATGTTCCTTACTCCTGTTGGCTTGATGG730.11438062110244117No Hit
GTTTGGGGGTCACTTGCTCTGAGACAGGTGCGGCAAGTCTACTGCGGGCTGG730.11438062110244117No Hit
GCACTCGGGAGAACATTTGAGTCGTTTATATATTGCTTATCAGTTTTTGCTG730.11438062110244117No Hit
CTATAAGGGGGCTGGGCGGAAGCTACCATGGCGGCCGAGAGAAAGACAAAGT720.11281376327912004No Hit
CATAGCGGGCTGCAGTAGGACACTGAAAACAGCAAGAACTTCAGGGTGAACA720.11281376327912004No Hit
GCGTAGCGGGCAAGGAGCTGGTTCTGCTGGTTGGACCAAACCTCGTGAGCCA720.11281376327912004No Hit
TAGACATGGGCTTACTGAGAGACTTTTAAGTAAGATCTATAAACTCTCTTTG720.11281376327912004No Hit
ATTGTCGGGTGGCTAAGGCAGACACACTGACACCTCCCGAAGTGGACCACAA720.11281376327912004No Hit
TAGAAAGGGAAATTTGCATGTGACCTCATCTAGCTATGAATTCTGGGAAGTC720.11281376327912004No Hit
GAAAACGGGCGCAGCGGGTGTAACAATCCCAAATCAAATCTGTCTCCAGGCT720.11281376327912004No Hit
CCTGAATAAGTCGTCCTACAAATATGAAGCAGACACGGTGGACCTGAATTGG720.11281376327912004No Hit
GTGGGGGCACTCTGATTTAAGAAATGATTTCTAGATCTTACTGGTGGCCAAT720.11281376327912004No Hit
ATGGCAGGGAACCAGGAGGGAGACGCGGCCTACAGGAAGGCCTTGGACCTGG710.11124690545579895No Hit
TGTAATGGGGGTCATTTCCGGGCGTCACGTAACGGAGTGGCCAACGGCCTGC710.11124690545579895No Hit
TGCGATGGGTCATTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATA710.11124690545579895No Hit
CTTGTTGAGGGACTGAGGGTAGTTATGTTAAAGTTTTGCGAACTTAAAGGGC710.11124690545579895No Hit
TATAACGGGTGCTACTCGGTTAATGAGCTTGGCAGGTTCTTTGTCTCACTGA710.11124690545579895No Hit
TCAAGCGGGGATCTCGGTGTGGGAATTCAATGATTTACAAAGTTGTGCAAAC710.11124690545579895No Hit
TATTGTGGGATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATAGTACC710.11124690545579895No Hit
CTCAAGGCGGAATGTTAATTTATTTAAAAATTGGCCTGAATAATATGTAAAA710.11124690545579895No Hit
ATAACAGGGATGGAGACCCTGAGATCTTCCCCACCCCCAATCATGATGTCTT700.10968004763247782No Hit
TTGTTTAGGGACTGGCTGTGGCTGGAATCTGCCTTTTATCACAGCTGTCACC700.10968004763247782No Hit
TTGGCTGGGAAGAAGACAGCTTTTCCGCTGAAGCTTAAACAGGCATTAACGC700.10968004763247782No Hit
GTAAGAGGGGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTG700.10968004763247782No Hit
TAATTAGGGGGAGCTCCAGCCTGACATCAAAAAAGGCAGTTACCATTAAACC700.10968004763247782No Hit
TAGACAGGGGAGGTGACTGTGAAGGAATCAGGATGCAAGAAGAGTCAGTAAA690.10811318980915671No Hit
GTTAGTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG690.10811318980915671No Hit
CAGTTTCAGTCAGGGACATATTTTGTGCAATATTTCTGTGATTGTGCCTATG690.10811318980915671No Hit
GACTTTGGGGCCACAGAGCCGAAGCCCGAGCTGCCGCCGCAGCCACAGCCGA690.10811318980915671No Hit
AATCAAGGGTATTTAAAATTTGCCCTATTTAAGCTGAAGCCTGGATAAACTG690.10811318980915671No Hit
GTTGTTGGGGCAAATCCATAAGCCTATGAATCAACGAGTTAATCAAAATGGC690.10811318980915671No Hit
TTCGGTTGGGTTGGTCAGCTTCTCTTTCTTTATCTTTCTTTCTCCTTTTTTA690.10811318980915671No Hit
TTTGGTGGGAAATGAAAGTTGTCATGGATAAAGGTCACCACCCAGTTAGGAG690.10811318980915671No Hit
TAGGTGGGAGAATATGAGAGTTACACCCAATACTACTATTATGATTATTAGT690.10811318980915671No Hit
CGCTCAAGGGCAAAGTAAGCCATGTATCTCAGGAAGGTAACTTCATTTTGTC690.10811318980915671No Hit
CGTTTGGGGACAGCAGTGATTAACCTTTAGCAATAAACGAAAGTTTAACTAA680.1065463319858356No Hit
GTCACTGGGATGATTTCATGATTAGACAATGTGGAATTACATAACAGGCATT680.1065463319858356No Hit
GACAGTGGGAGGCTTTCCCAGGCAGCTGGCTAGTTCATTCCCTCCCCAGCCA680.1065463319858356No Hit
ATGGGTGGGGTCTAAGGGGACTGGCTGGCCACATATTTGTTCCTTGCCCATA680.1065463319858356No Hit
TAACAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG680.1065463319858356No Hit
CGTCTTGGGAGTGAACTTGACCAACACCCTATGGATAGAGTCTTGACACATT680.1065463319858356No Hit
GACCATGGGATGCATAAAAATTTGACTTTTGTGCAGTGGCATCAAAAATTTT680.1065463319858356No Hit
GTTTTTGGGCCGATCTAAAAGCGGGTAAGGCACACTGGTGAAGTTTACAACC680.1065463319858356No Hit
TATTCTGGGAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAA680.1065463319858356No Hit
CTTCCTGGGCACCGATCGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT680.1065463319858356No Hit
CCAGCCTGGGTAACAAGAGTGAAACTCTGTCTCAAAACAAGATAAAAAGAAA680.1065463319858356No Hit
GTCGATTGGGGATCAAAACATATCAACAGCCTCAAGATTTTTAAACTTGTCC680.1065463319858356No Hit
GGATTTGGGTGTTATGTGTGTTGACTGGGAAGAATTCTGGGAGGAGATGTGA680.1065463319858356No Hit
TTGAAGGGGGACCTATATCCCCTGCACACTCATTGTCGTCCTATCCTGGGTG670.10497947416251449No Hit
CAGACTAGGGATTCCTCTGTACTTTCCTTTCGCTATTCATCAATTTCCCATT670.10497947416251449No Hit
GGCCTCAGACTTGGCCTTGTGGATGGGCCCCTTACAGTATTTGCTGACTAGT670.10497947416251449No Hit
GTAATAGGGATGGCTAATATGCATAACAAGGAAAAATGATGTAGCTTATAGG670.10497947416251449No Hit
GGAACCGGGACAGCCTTAATTCACAATTGGCAAAGGCAGTTTACTCAAAGGA670.10497947416251449No Hit
ATGTGAGGGAGAACTGACCCAGATGTTCACAAACAGGGCACTCAGAGATACC670.10497947416251449No Hit
ATGTCTGGGGATACTGTGGTAGATTTCTAGAAATTCATTCACATTTAAGACT670.10497947416251449No Hit
TCAATGGGGAGCAGGATTAAACCTGAATAACTTTTGGCTGTTGTGCTAATAG670.10497947416251449No Hit
TATATCGGGACCCCGTGCCAGTGTCTTGGAAGTGTCAACGTGTTTTTGGATG670.10497947416251449No Hit
GTAATTGGGAGAATTGACTGCAGTTGAACAGACTAGAAGTATTTGTGGGAGG670.10497947416251449No Hit
TGCAGTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA670.10497947416251449No Hit
AGAATGGCAAGGATGGTGAAAAAAACTAACATCATTGCATCCCTGTTTTTTT670.10497947416251449No Hit
ATCGGCGGGAAGAGGCGAGATAACACAGTTATCAACTAAGTATGAGTAGGAA670.10497947416251449No Hit
TCCCTAGGGGAGGGTTTATCAGGTGGCTGCCACAGGATTGGCAGTGGAACAA670.10497947416251449No Hit
AGCCTAGGGTTAGTAATTTTGAGGCATAGGGTAGCTTACGTAGTGGAGGATG670.10497947416251449No Hit
CGTCGGGGACTTTTAATTTAAGAATTCTGTGTTTCTTGCTGCCAACACTTTG670.10497947416251449No Hit
AGACCTGGGAGAAAATCGAAGTTTACCATGTCTGTAATGTGTACATGAAGTG670.10497947416251449No Hit
CTGCGGGTTCAAATGTGATTTATGATGAACAGTCACCATTACCCCCATCTTC660.1034126163391934No Hit
CACGAAGGGGAACATTCCTAATATACTTATGTAAATGTCCTGTCTTAGTCTG660.1034126163391934No Hit
AGTAGGGGCAGAGCTGGCATCCATAATGATATTTTGTTAATTCTAATTGGTT660.1034126163391934No Hit
TGTTCAGGGGTTGCTTTCCATGTTGCATTATTGGCAGGTTATTCTAAATTAT660.1034126163391934No Hit
TCACACGGGATTGTGTGCCTCTCTCTATTGCCTTATTTTCCCTATTGTTCAT660.1034126163391934No Hit
ATAACAGGGTATCACACCACGTCTTTTCAAAAACTAAAGAGAATTCAAAAAG660.1034126163391934No Hit
TTAGTAGGGGTGTATTGAAAATTTCAAAGTAAAGGATATTATTAGAATTCTG660.1034126163391934No Hit
TGTCCAGGGTAAGTGGACAATAAATGGTGGGCTCGATCTCCTCAGGACTGTA660.1034126163391934No Hit
GCAGTGCGTGTGAGGGCACCTTCTAGGTGGGCCAGGGATGCAGCTATGGAGA660.1034126163391934No Hit
CTTCAAGGGCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGG660.1034126163391934No Hit
TAATCAGGGGGTGGCTGTTGCGGATGGGGCGTAGGTGGGCGGTGTGCCCACA660.1034126163391934No Hit
GGCGGTGGTAGGGATCACGCCACTGTACTCCAGGCTGGGAGACAGTGAGATA660.1034126163391934No Hit
CGTGACGGGGACGGAGGGCGGTGGCGGCGGGCTCCTGCGAGAAGCAAGCGGA650.10184575851587227No Hit
TATTGAGGGCGATCTTAACACGGGATGACCCCAATACGCAAAACTAACCCCC650.10184575851587227No Hit
ATCATCGGGGGCCATTGGCAATTGCGCAGTTTTAGGCTTTTCTGTTCTTCCC650.10184575851587227No Hit
AGTTCAGGGGTCTTCCTCGATCCCGTGGTGCTCCGCGGCGCGGCCTTGCTCT650.10184575851587227No Hit
TGGCTTGGGGACAATTAGGAAAAAAAGCTCAAATTATTGTACTTGTTAAAGG650.10184575851587227No Hit
CGCTAGGGAAGAATGAAATATCTTTTTAAGAAAATTATATGAGAATTGCTAA650.10184575851587227No Hit
TAAGAAGGGATCCTAGGCCTACCCGCCGCAGTACTGATCATTCTATTTCCCC640.10027890069255116No Hit
CTCTCGCAGGGGTCTCCATCTTATAGATGAGGAAACTGAAGACAAGACCTTG640.10027890069255116No Hit
TCAGGGGTTTATAAAAGTAGTAGTTCAGAAATTATAATTTAAAAAGTAACTA640.10027890069255116No Hit
CATTGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC640.10027890069255116No Hit
GTTGCTGGGGCTTCCAAGTCAGCTCCATCCATCCCCAAGGAGAACTTCAGTT640.10027890069255116No Hit
TTTGCAGGGAGGCAACCCAAACTTAATGCATTAAAAACAAACCAAAACAAAA640.10027890069255116No Hit
TCTTGTGGGAAGACAGAGGGACTGATAATAGTTACGGCTTGAGTCAACCAAA640.10027890069255116No Hit
TAGACCGGGGTAGAAAATTACTGTCTACCAGAAGCTATTTCATTTATTCAGA640.10027890069255116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)