FastQCFastQC Report
Sat 31 Dec 2022
SRR5849378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63012
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4010.6363867199898432No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3270.5189487716625405No Hit
CAGCATGGGCAAGTACTGAAACTCTATACCCATTAAATAACAACTCACTATT1050.16663492668063226No Hit
AAAACAGGGTGTGACAGTCAGAAAACACACCTGGAATACGATGCAGCCCACC1010.16028692947375103No Hit
GTTCCCGGCTACGAGGCCTAGACCAGCAGCTGGATGATCTACAGACCAAGTT1010.16028692947375103No Hit
CATCCAGGGACTTTATGGCATATAAAAATACTAATTCAAGGTGAATCACAGA1000.15869993017203074No Hit
CTCTAGGGGCAACAGCAGTGATGAAGAGGAGGAGGAAGAGGAAGAAGAGGAA980.1555259315685901No Hit
CACATAGGACGATAAAGTGATAATTTCTTCATCTCTCCATCTAAGGTTCTTT950.1507649336634292No Hit
TCGAACGGGCGATCTGGTAACGGGTGCACGAGGGAGTCAGTGTTGTCCAAGT950.1507649336634292No Hit
CCAAATGGGTTCATAGCGGGGATGGTGCTACATATATCTATGCAACTGAAAA930.14759093505998858No Hit
GTAGGTGGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGT930.14759093505998858No Hit
GAACTGCGGACTTTGGATCAGATGTGCAGTCGGAGAAGGGAAAATGACATTG930.14759093505998858No Hit
TCGACCGGGATTTGCAGTTCCTGAAGACTTCAGAGTTTTATCCCAAAGAAAT920.14600393575826826No Hit
TCAGGGGTTTATAAAAGTAGTAGTTCAGAAATTATAATTTAAAAAGTAACTA910.14441693645654796No Hit
TTAACTCGGGGGCTGCGGTGGCACCAGCCAGGAGGCGGAGTGGAAGTGGCCG910.14441693645654796No Hit
CTTAGCGGGCCGATCTCGGGTAGGGAGCATGAAGAAGAGGAAGATCTGGATC900.14282993715482767No Hit
CTGCACGGGCAGATATTGACATGGAAACAGTTAAGTCCCAAAACAGCTCTAT890.14124293785310735No Hit
TGATAGGGGGAAGTTTTATTCTTGTATATTTAGATTTGTATGCTTGTGAGTA890.14124293785310735No Hit
ACTCAAGGGGACGGCCTCGCCTCGGTATCGCAGCGGGTCCTCTCTATCTAGC880.13965593855138703No Hit
GTACAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG880.13965593855138703No Hit
CTAGCAGGCTGTCAAATGTACCAACATTTTCGAAAGAATTCATTCAAATCTC870.13806893924966673No Hit
AAGCTGGGGAGATTCAAATCAAACTAGATGTTAATCTACTTATAAGGAAAAA870.13806893924966673No Hit
TCGGAGCGGGCATGTGCTACTTGGAAGGATGGAGATAAAAGGTAGAGAAAAT860.1364819399479464No Hit
AGTTTAGGGTAAAGCAGTTCGTAAGAAGGCAACAAGTTCTTCTCCTCTACAG860.1364819399479464No Hit
GGCGCGGGGTGAAGGAATTATGGATGTTTTTATTTTAATTCTACTTTGCTGT850.13489494064622612No Hit
ATGGCAGGGAACCAGGAGGGAGACGCGGCCTACAGGAAGGCCTTGGACCTGG850.13489494064622612No Hit
TGCAGTGGGCCTACTTGATTAAAAAATATAAAGAACTGAGTGTTTATTTCCC850.13489494064622612No Hit
TAACCAGGGTTGAAGCAGCTGGCTGAGAAACGAGAGCACGAGAAAGAAGTGC850.13489494064622612No Hit
AGTCCAGGGCAGTCCATTTATGGAGACAAATTTGAAGATGAAAATTTTGATG850.13489494064622612No Hit
TAGCCAGGGTTACACCATTTGCCCACTTCTATGAATTTTATGTATAAAATTT840.13330794134450583No Hit
CTCCTTGGGGAGATACGGACTTGATGGTGCTGTTTAATCAGTTTGCTTCCAA840.13330794134450583No Hit
TCATGGAGGGCTATTATTGCCATGGAGATTTTAAGAGATGATGCTATATGTG830.1317209420427855No Hit
GGATTTGGGTGTTATGTGTGTTGACTGGGAAGAATTCTGGGAGGAGATGTGA830.1317209420427855No Hit
TTATGCGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC830.1317209420427855No Hit
TGATCCGGGGAAAAAAATGAATGCCATTTTCAAGTTCCCAAATTACTTCTAT820.13013394274106518No Hit
AAAACCGGGGACCACGATGTTACATCCGCAAAGGATGGTGATTACTGGAGAT820.13013394274106518No Hit
TTCGGGGACTTTTATTGACATACTGTAATACTTATGGGTGAAATGATATATC810.1285469434393449No Hit
AATGAAGGGAGAGAGCTTACTTGAAGAAATAATGGTCAAAAATTTCCCAAGT800.12695994413762457No Hit
GACAGCGGGTAAGAGGAATCCCTGAGAAAATGTAGATAAATTTAATCATATA800.12695994413762457No Hit
TAGGTGGGAGAATATGAGAGTTACACCCAATACTACTATTATGATTATTAGT800.12695994413762457No Hit
ATTGTAGGGTCTGCTTTTTGTAAAGCGTAAAAACATCACAAAGTAGGTCATT800.12695994413762457No Hit
AGTAGGGGCAGAGCTGGCATCCATAATGATATTTTGTTAATTCTAATTGGTT790.12537294483590428No Hit
TTTAAAGGGGTACACATTTGTTTCAAGTGCAGATGGAGCATTTACCAAAATT790.12537294483590428No Hit
ATAACAGGGTATCACACCACGTCTTTTCAAAAACTAAAGAGAATTCAAAAAG790.12537294483590428No Hit
GTAATTGGGAGAATTGACTGCAGTTGAACAGACTAGAAGTATTTGTGGGAGG790.12537294483590428No Hit
TAATCGCGGGAATTCCTAAGAGCCACTGACATCCAAAAAATTCATTACTTAC790.12537294483590428No Hit
CAGACTAGGGATTCCTCTGTACTTTCCTTTCGCTATTCATCAATTTCCCATT780.12378594553418396No Hit
TCTTAGGGGGAACTAAACTGCTATGATAAAAAATGGGGAATAGAGGAAATAA780.12378594553418396No Hit
TCTGGTGGGACATGCTGGAAAAACAGTGCCGATTGTGAAAGGAGGGGAGGCA780.12378594553418396No Hit
AGAATGGCAAGGATGGTGAAAAAAACTAACATCATTGCATCCCTGTTTTTTT780.12378594553418396No Hit
AAACCAGGGGTCGCTTGGAAGAGAAGACTAATCAGATGGCTGCTACCATTAA780.12378594553418396No Hit
CCAGCCTGGGTAACAAGAGTGAAACTCTGTCTCAAAACAAGATAAAAAGAAA780.12378594553418396No Hit
CGTACGGGGCACAGTGGAAGGTCTGGATGGAGAGCGGCCACTGCAGACTGGG770.12219894623246366No Hit
ATAACAGGGATGGAGACCCTGAGATCTTCCCCACCCCCAATCATGATGTCTT770.12219894623246366No Hit
AGTAAAGGGAAGAAACTGTTCGAAAAAGAACAAAAACACTTCCCTCGTTATT770.12219894623246366No Hit
GCCACCGGGACTAAAAGACCAAGGGAGAGTCAAAGAAATGGAGGTGTAGAAG770.12219894623246366No Hit
TAGTATGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAG760.12061194693074334No Hit
GCTTAAGGGGGTTTTAAGGATCAAATCTATGAGATTTTCCAAAAACTAAACA760.12061194693074334No Hit
ATCCTTGGGACCAGCCAGAACGCCTGAACGCAGGCACATACTTCCTATTCTA760.12061194693074334No Hit
GATGGTGGGATGAAAGCACAAGAATAAGGCTGGAGATGGTCCCAGATCCAAG760.12061194693074334No Hit
ATGGGTGGGGTCTAAGGGGACTGGCTGGCCACATATTTGTTCCTTGCCCATA760.12061194693074334No Hit
TAATGTGGGATGACACCAGAGGTCTACAGGAAGGCGTGGAGCTGCCAAAGCA760.12061194693074334No Hit
AATCACGGGGACTTGATTAAGAATTATTATGGAGAAAAAATTGGTATCTATT750.11902494762902303No Hit
GTGCCGGGGATGGAATGGTAGTTGAGAAGAAAACTATTTGCACACAACAGAT750.11902494762902303No Hit
TTGTCAGGGAGCCAGATTGTGTCCTCCATCACTGCTTCCCTGAGATTTGATG750.11902494762902303No Hit
TCGCGCTGGGATGCATATTTACCGTGATGTCTGGACCGTACCTGTGCTCCTT750.11902494762902303No Hit
ACTTGTGGGCTCCTGCCCTGTCTTGGCACAACTCTGGATAGATTGCAGGTGT750.11902494762902303No Hit
ACAATGGGCACACAGGGGACCATAAAACTTTGAAAATTGAAGTACAGCATTT740.11743794832730274No Hit
GCAGTTGGGACTTGTTTTGTTATGAAATTTAAATATGTAAGTTGATAAAATG740.11743794832730274No Hit
CAGTCAGGGAATGAGGATGTAAGCCCGTGGGCGATTATGAGAATGACTGCGC740.11743794832730274No Hit
TTAGTAGGGGTGTATTGAAAATTTCAAAGTAAAGGATATTATTAGAATTCTG730.11585094902558243No Hit
TGGATAGGGATATGGAGGAGGCAGAGGCTGTCTGTGATCGAGTAGCTATCAT730.11585094902558243No Hit
AAATTTGGGACCTTTGGTTAATCCTGCCTTATTTCAGTAAAGAAAATTTGTA730.11585094902558243No Hit
CAGCCAGGGAAGATTTGATCTACCATTTAATTTAATAGGTACTGTAAATTAG720.11426394972386211No Hit
ACGCGAGGGAAGTGAAGGGAGAAAGGAAAAAGCTTCCTATTCTTACCCTTTC720.11426394972386211No Hit
GTGGGGGCACTCTGATTTAAGAAATGATTTCTAGATCTTACTGGTGGCCAAT720.11426394972386211No Hit
AGGCCGGGAGAAAACTGGAGTAAGAAGTGCAATATCAGCCTAAAACAAGCAG710.11267695042214182No Hit
GTAATAGGGGTATGTGTGTGTTTTGTAAAATCTGTAAATAGCACATGACCAA710.11267695042214182No Hit
AATCAAGGGTATTTAAAATTTGCCCTATTTAAGCTGAAGCCTGGATAAACTG710.11267695042214182No Hit
GAAAGCGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC710.11267695042214182No Hit
TTGGATGGGGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAA710.11267695042214182No Hit
CATCGCGGGCTGACCACTTGTGGCTGTCATTGGACTGATTTGCCCAGATGAC710.11267695042214182No Hit
CTGCCTGGGATTGTTTTACTGTAATTAGTGTGTACTTATATTTTATGTCCTT700.11108995112042151No Hit
TGTAATGGGGGTCATTTCCGGGCGTCACGTAACGGAGTGGCCAACGGCCTGC700.11108995112042151No Hit
CTATATCGGGGGGAGAGATTATAAACCACTGGGGTAAAAGGATAGGCTTCTT700.11108995112042151No Hit
AGCAATGGGATCAGGAATGCTGGAGGTGCATTTTAAGTTTTAATAATAAGTG700.11108995112042151No Hit
ACGTCCGGGGGTTGAACTGCCCTTAACGTCACCACTACTAACGTCTATGTTG700.11108995112042151No Hit
TCACTTGGGGAGCATGCTGTTACTATTCTTGTTTTCTAGAAATTTTAATAAG700.11108995112042151No Hit
TATTCTGGGAGAAATAAACATGCTAGCTTTTATTCCAGTTCTAACCAAAAAA700.11108995112042151No Hit
AGCTTAGGGAAGTACACAACTCAGTTAAGTATTCACATACACACAGAAAATA700.11108995112042151No Hit
CTCAAGGCGGAATGTTAATTTATTTAAAAATTGGCCTGAATAATATGTAAAA700.11108995112042151No Hit
GAACTGGGGTTGTCTTTCCAAAGCGCTGAGGCTGTGCACCTATTCTGTAGTT690.10950295181870119No Hit
TCTCTGAGGGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCTAAAAA690.10950295181870119No Hit
TATAACGGGAACCACATTTATTTAAGAATGTATTCCAATATCAAACAGCAAA690.10950295181870119No Hit
TTGGCTGGGAAGAAGACAGCTTTTCCGCTGAAGCTTAAACAGGCATTAACGC690.10950295181870119No Hit
TAGAAAGGGAAATTTGCATGTGACCTCATCTAGCTATGAATTCTGGGAAGTC690.10950295181870119No Hit
AAATCCGGGATGCCCAGGGTTGTATTAAAGAATTTTTCTGCAAGACATTTTT690.10950295181870119No Hit
TAAGTGGGGCATATCACATAAATAGACTCAAACAATATATGGCTTTTTTTTT690.10950295181870119No Hit
GGTGCTGGGCGATCTAGGCAGGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTT680.1079159525169809No Hit
TATTGAGGGCGATCTTAACACGGGATGACCCCAATACGCAAAACTAACCCCC680.1079159525169809No Hit
GCATAAGGATTCCTACTTGTACGGACTTTGAAGTGATTCAGTTTCCTCTACG680.1079159525169809No Hit
CTTCAAGGGCAAGTGATGCTTACAAAAAAGCCTGGGGCAATAATCAGGACGG680.1079159525169809No Hit
GCACTCGGGAGAACATTTGAGTCGTTTATATATTGCTTATCAGTTTTTGCTG680.1079159525169809No Hit
ATGGCGGATGGCATAATTTGGAAATAATGCTTTCAACTTCCTTCTACTTCAT680.1079159525169809No Hit
TCACTGGGGGCAAGAAGCTAATTCATATGCTTTAAACCAATGAAGGCTTGTC680.1079159525169809No Hit
TAGTTGGGGATCTGCGGCTGGGAAGAGGAGGAAGCGGGGACACAGTGGGGAC680.1079159525169809No Hit
AGGTGTGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC680.1079159525169809No Hit
CTGCGGGTTCAAATGTGATTTATGATGAACAGTCACCATTACCCCCATCTTC670.10632895321526059No Hit
ATACTGGGAACTCAAACAAATTTACAAGAAAAAAACAAACAACCCCATCAAA670.10632895321526059No Hit
TGTACCGGGATGGCTGGCCTGGTTTGTTCCTGGAGTCCGCACGGTGGCTGAT670.10632895321526059No Hit
CTAGTGGGGATGACCATAACATCTATTACTTGGGACCATCTTGCCAAGTGCT670.10632895321526059No Hit
TCGTTAGGGACACGATAAGTTTACTCCTCCATCTCAGTAATAAAAATTAAGC670.10632895321526059No Hit
ATAGTAGGGATTGAAACTGGTGAGTTCTGATTATTAAATATTCTCTGTAAAT670.10632895321526059No Hit
CATATCGGGAGATTATTTAAAGTACATGGGAAGATGTGTAAAGGTTGTATGT670.10632895321526059No Hit
GTTATGGGTCTACGAAAGGGCTTCGATGACGTGCAAGGTCATGACCTCATGG670.10632895321526059No Hit
ATGTCGGGGGGATACCATGCAGTGGTTTTAATCCCAGGAAAAAAACTATCAA670.10632895321526059No Hit
CAGTAGCGGGCACATCTGTTGATTCTAAAGTATATTTATGTGTGTGTGTATG670.10632895321526059No Hit
GAGCCACTGGAGGAAGCTGTTTTGAGCCAGGGTTTTTTGGCCTAAAGCCAGC660.10474195391354027No Hit
ACAAAAGGGAAGAGGGTTTCTGGAGGATGGCATAGCAGTTGTTCTGAAGCAG660.10474195391354027No Hit
TGTCATGGGGTCCTAAATCACCCTGACTCTGACCAGTCTTTCAGTTCTCCAA660.10474195391354027No Hit
TTGAGAGGTTAAGGGGTTAATTATGTTTTAATGAAAGCACAAAATATTTATT660.10474195391354027No Hit
AGCCTAGGGTTAGTAATTTTGAGGCATAGGGTAGCTTACGTAGTGGAGGATG660.10474195391354027No Hit
TAGACCGGGGTAGAAAATTACTGTCTACCAGAAGCTATTTCATTTATTCAGA660.10474195391354027No Hit
CTGGATAGGGGAGTCCAGATCAAAAATGGCGGTAGTTGGTGTGTCCTCGGTT660.10474195391354027No Hit
TGACCGAGGGAGACCCACCCTGGTGTAGACTCTTTATGGGAAAAAAGGAAAA650.10315495461181998No Hit
TCATGAGGGACTTTTGTTCATTTTAAATGGCAACTTAAAGATATGGATGCAT650.10315495461181998No Hit
TGTTCAGGGGTTGCTTTCCATGTTGCATTATTGGCAGGTTATTCTAAATTAT650.10315495461181998No Hit
AGCGCAGGGATTGTCTAAATGCATTTAAATCTGTTTTATTCAAAGAAAAGCT650.10315495461181998No Hit
ACAGTTGGGCGCCCTCTATCCGTAGAGTAGTCATGACACTATACAGATGGTT650.10315495461181998No Hit
GTAAGAGGGGAAGTAGATGACTTTGAGGACTTCATCTTCAGCCACTTCTTTG650.10315495461181998No Hit
GAAAACGGGCGCAGCGGGTGTAACAATCCCAAATCAAATCTGTCTCCAGGCT650.10315495461181998No Hit
ATAACGGGGATCTAAAGCAGGGATATAAATCTGTAATAGATTTTAGAATTGA650.10315495461181998No Hit
AAACACTGGGAAAAGAATATGCAGAAGTTTTTGACTTGGGAAACAGGGTTAC650.10315495461181998No Hit
TACCTAGGGGTTCTGATGTACTAAGTGACTATGGCAGATACCGATACTATGT650.10315495461181998No Hit
CTATAAGGGGGCTGGGCGGAAGCTACCATGGCGGCCGAGAGAAAGACAAAGT640.10156795531009967No Hit
AACCTAGGGAACATCGGCATTATCCTCCTGCTTGCAACTATAGCAACAGCCT640.10156795531009967No Hit
GTTAATGGGAACTGCTATGACGGTTAAGAATGTCAGTATACAAGAAGGAATA640.10156795531009967No Hit
AGCTGGGTCTGCATAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTG640.10156795531009967No Hit
TTGTTTAGGGACTGGCTGTGGCTGGAATCTGCCTTTTATCACAGCTGTCACC640.10156795531009967No Hit
CTAGTTTGGGGATATCAAAAAACTTGGGGATAGAAAGAGGCTTTTTTTTCCC640.10156795531009967No Hit
GCTCAGGGGATTTTGCAGAAAGGTACTGTTAGTGACTGATAAGGATGCCTTT640.10156795531009967No Hit
GTTTGGGGGTCACTTGCTCTGAGACAGGTGCGGCAAGTCTACTGCGGGCTGG640.10156795531009967No Hit
CTCACTGGGGGGATCTTAGGCAGGGTAATTGGAGAGCTAAGTAAGTAAAGTT640.10156795531009967No Hit
TTCGCAGGGGGATGGGCTCTCTGACAAGGAGTGTGCCGTGCGCCGTGTGAAG640.10156795531009967No Hit
TAATCAGGGGGTGGCTGTTGCGGATGGGGCGTAGGTGGGCGGTGTGCCCACA640.10156795531009967No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)