FastQCFastQC Report
Sat 31 Dec 2022
SRR5849374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40037
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT3030.7567999600369658No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC2730.681869270924395No Hit
AGGCAGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG680.1698428953218273No Hit
TTCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC630.15735444713639882No Hit
TAGACAGGGGAGCACTGGAGAGTGTCATGGAGGCCTACGAGCAGGTCCAAAA560.13987061967679898No Hit
CGGATGGGAACCCAGGATTCACAGTGAGAATGGGAAGAAGGGAACTGAAGTA540.13487524040262758No Hit
TTGTACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA520.12987986112845618No Hit
ATACTAGGGACAAATCATCATCTTACAAAGGACTAATATCCAGAATCTACAA520.12987986112845618No Hit
GCTAGTGGGAGGACTACGTGGGGCCGCTGCCGGCTCCGGGTTGCTGGGCGGC520.12987986112845618No Hit
GCATGTGGGGGGACTAGATTCTAAAATTTATTTGGGACCATGGGAATGATAG520.12987986112845618No Hit
TGAAATGGGGAGGACAAGACTTTATACATTTGGAATTCTATATTTAATAGCT510.1273821714913705No Hit
ATCACAGGCGCCCTGATCGGCGCACTGCGAGCAGTAGCCCAAACAATCTCAT510.1273821714913705No Hit
TGTTCCGGGTATGGTATCTGCATTATAATTCTTTTTAAAGTACAAGTTATTT500.12488448185428479No Hit
CGTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC500.12488448185428479No Hit
AAGGCTGGGAACCAGACTCAAAAAGAAAGAAAAGAAATCAGAATGTTCAAGA500.12488448185428479No Hit
AACTGGGGGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGCCCTGGGCCGGGCC490.12238679221719909No Hit
TTCAAAGGGCAATACTTAATTTACCAGGTGCTGTTTATGTTGTAGGCACCTA490.12238679221719909No Hit
GAGTCCGGGCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCA490.12238679221719909No Hit
CGACAAGGGGATCTGTTTCAGGGGGAAGCGATGGAGCTCCTCACTGGACTCG480.11988910258011339No Hit
TATTCCGGGGGGACATGAACAGTCCTATAGATGAAAAGAGGTGCCTGTCTGA480.11988910258011339No Hit
AGTTTTGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC480.11988910258011339No Hit
TAACATGGGGACTTAAGTACACATAAGAATTGAGGAGAGGGTGGGTCTCAAA470.11739141294302768No Hit
GCTGCTGGGGTGGTGAGAGTGGAGGAGGAAGAGGAGGAGGAAGAGGACTTCT470.11739141294302768No Hit
CTGTGGGGGGAAAAATTAATTAGAGAAATCACTCTTACTGTTGTTAAAGTTA470.11739141294302768No Hit
TTTGAAGGGTCTGGATCCGGGATCCATTAGTTCTCATACCATGAAGAAATTA460.11489372330594201No Hit
CTTATCGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAAT450.11239603366885631No Hit
GCTTAAGGGATGGCTTATAAAAAATATGCTACATTAGGAATTCCTTCATGTA450.11239603366885631No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT450.11239603366885631No Hit
AAAAAGGGGACGCCATCCACTTCTGCAAGTCCATAGACACAGATGACTACGC450.11239603366885631No Hit
ACTGCCGGGTGGATTTGAAAGAATTCAACTTTGGGACCACACACTTTGAATT450.11239603366885631No Hit
ACTGGCGGGATAGAATTTTTGTCTAGAATCCCAAACTGGCAACGATAGTCTC450.11239603366885631No Hit
TTCTAGGGGAGGTCACAAACCTGAACAGCAGCATAGCCAAAATGGGAACGGA440.10989834403177061No Hit
TTGCAAGGGATTATTTAATCTTGCAAAAATGGTTCTGTGCACTTGGATGTGA440.10989834403177061No Hit
CTAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA440.10989834403177061No Hit
TACTCCGGGATCCCTAGCATGCATGGTGGCTCCAACCTCTGGGGTGATACCA440.10989834403177061No Hit
AATCAAGGGCAGCTCACTGCAAACTCTGCCTCTGGGGTTCAAGCAATTCTCG430.10740065439468491No Hit
TTTTGTGGGAGAATCTTTTTATTTGCTGATTTGTCTCTTGTGTATTTTCTTT430.10740065439468491No Hit
TTGCCTGGGCCCCGGGGGTGGAGGCTGCAGTGAGCCGAGATCTCACCATCAC430.10740065439468491No Hit
CCAACAGGGAAGATATCAATAGAGAAAAATCAAAGAAATCAACTAGTTCTCT430.10740065439468491No Hit
AAAGTTGGGGGACCAAATTGCTTTACAGTTTTTTTCTTTGTGTAGTATCTTG420.1049029647575992No Hit
GTAATTGGGGAAAAAACAAATATCCCAAAAAGTCAACCTAAATGTTTTGGAT420.1049029647575992No Hit
ACAAGCGGGTGATTACTAAATCAGAATTCATTTAGCTCACCACATCTCTTGA420.1049029647575992No Hit
TAGTACGGGGTCTTAACGTGAATTGAGTCTCCAAGGTCTCCATGTGAAACAA410.10240527512051353No Hit
AATCATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC410.10240527512051353No Hit
TTTCAGGGGATGTGAGGAAACTATCCAAAGTCTGGCAAAGAACCACAGGAAA410.10240527512051353No Hit
ACTTTTGGGAAGAGGGCTGGATAAATGGTGAATATTTTTTTAAAACATTTCC410.10240527512051353No Hit
GTAATAGGGCTGAGGAAGAGGCCCGTTTGAAGAAGAGTGCAGATACACTTTG410.10240527512051353No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)