FastQCFastQC Report
Sat 31 Dec 2022
SRR5849373.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849373.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39767
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC2900.7292478688359696No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT2570.6462644906580833No Hit
AAGGCTGGGAACCAGACTCAAAAAGAAAGAAAAGAAATCAGAATGTTCAAGA660.16596675635577238No Hit
TTCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC600.1508788694143385No Hit
CGTGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC570.1433349259436216No Hit
TAGACAGGGGAGCACTGGAGAGTGTCATGGAGGCCTACGAGCAGGTCCAAAA560.1408202781200493No Hit
GTATTAGGGCGGGGGGCGAGAGCGGCTAGGAGGGCCCGGGCCCGGCGTGGGG550.13830563029647697No Hit
ATGTCAGGGGACTCGCGAGGGCGTAAAAGTTTGTCTCAGCTCGAGCATTCCT530.13327633464933236No Hit
AGTTTTGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC510.12824703900218773No Hit
ACGCTTGGGGGAGTGTTTGGGGACATGGCCCAGGACACGGGGATCCCTGCTG500.12573239117861543No Hit
GTAATTGGGGAAAAAACAAATATCCCAAAAAGTCAACCTAAATGTTTTGGAT500.12573239117861543No Hit
TTGCAAGGGATTATTTAATCTTGCAAAAATGGTTCTGTGCACTTGGATGTGA490.12321774335504314No Hit
ACGCCTGGGGCGTGGACTGCCGAGAATATTGAATGTCAAAGAATTGCTGGAG490.12321774335504314No Hit
GAGTCCGGGCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCA490.12321774335504314No Hit
GCTAGTGGGAGGACTACGTGGGGCCGCTGCCGGCTCCGGGTTGCTGGGCGGC490.12321774335504314No Hit
AACTGGGGGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGCCCTGGGCCGGGCC480.12070309553147082No Hit
AAAGTTGGGGGACCAAATTGCTTTACAGTTTTTTTCTTTGTGTAGTATCTTG470.1181884477078985No Hit
ATACTAGGGACAAATCATCATCTTACAAAGGACTAATATCCAGAATCTACAA470.1181884477078985No Hit
GCCGCAGGGAGTCTCATCCATGTTGCCACCAAGAATGACAGAATTTCATTAT470.1181884477078985No Hit
GCATGTGGGGGGACTAGATTCTAAAATTTATTTGGGACCATGGGAATGATAG470.1181884477078985No Hit
GCATCAAGGGAGACTTAAATGTAAAACCTGAAACTGTAAAACTACGAGAAAA470.1181884477078985No Hit
AAAGCAGGGGCTTCTGCTGAGGGGGCAGGCGGAGCTTGAGGAAACCGCAGAT460.11567379988432619No Hit
TAGTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC460.11567379988432619No Hit
TATTCGGGGAGCATACCCTGTAAAAAGTGTGTGGTGGTTGGTAATGGAGGAG460.11567379988432619No Hit
AGTAAGGGGAAGTAAAGATCAATGGGTTCATACATACAAATTCAAAAGTTAA460.11567379988432619No Hit
TAGCAGGGGCTCCATTCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTA460.11567379988432619No Hit
ACAAGCGGGTGATTACTAAATCAGAATTCATTTAGCTCACCACATCTCTTGA460.11567379988432619No Hit
GCGAACGGGAGCTCTGAGGGTCTTTGTGAGTTCCCACCAACTTTTAATTATT450.1131591520607539No Hit
TGTGATGGGACTGACCTCATAGGATAAGTTGGGAAGTGGTAACTTCCTCCTC450.1131591520607539No Hit
TACTGGGATAGCAGAGAGAACTATGTAAATTATCTTGCAGAACAAAATAGAA450.1131591520607539No Hit
TGGCACTGGGGGACTCGAATGGAATGGAATGGAATTCACTCGAAAGGAATTG450.1131591520607539No Hit
GACTAAGGGATTGCCTGCCTTCCTGGCAGCTGATCCTTAGCATGCCCTTTTC450.1131591520607539No Hit
TTACCGGGGGCTGCTGCAGGTGATCACACAGACACTGCAAGACCTACTCAAG450.1131591520607539No Hit
TTACCTGGGCAACCCCACCTCCTGGAGATGCTGATTCAGCTGCCGGCCAACT450.1131591520607539No Hit
TCGTCTGGGGATTAATTGTCAGATTCCACTACACTTTCTTCCTCATAGGTAG440.11064450423718158No Hit
ACCCAGGCTGGGCCCTGCAGGTGCTGGGCCTTCAGGCCCAGTGTGAGCCTGC440.11064450423718158No Hit
ACAATAGGGGAAGGGGGAACAGGGAGCTACTCTGGGCCCAGGAAATATGTCA440.11064450423718158No Hit
TCGACAGGGACAGTCAGGCCGAGACAGGTGGATCACCTGAGGTCAGGAGTTC440.11064450423718158No Hit
CGGATGGGAACCCAGGATTCACAGTGAGAATGGGAAGAAGGGAACTGAAGTA440.11064450423718158No Hit
GTCAGAGGGGACTTTTGATTGCTACATACCACAAAGAGTTTATGAACTGAGA440.11064450423718158No Hit
GTAATAGGGCTGAGGAAGAGGCCCGTTTGAAGAAGAGTGCAGATACACTTTG440.11064450423718158No Hit
ACAATGGGCAGGGAGCACAGTTTTTCAGTACATATTTTTTTTTCTGCAGAGC430.10812985641360927No Hit
AAGCTCGGGAGCTACATGAGGACTCAGTTGTCTCACTAATATATGTGAAATA430.10812985641360927No Hit
GGGCAAGGGTCCCCCAGCTTATGCTTGGTGGCCTATCTGCATTGTTTTTCAT430.10812985641360927No Hit
TAACATGGGCATGGTGTCATACTGGCCGACCAGGGCACCTACGGCTGCGAGA430.10812985641360927No Hit
TTAGACGGGAAGTGAATTGTTGGAGAATGTAAACCGCCTCCACGTGGCGGCA420.10561520859003695No Hit
GTCTTTATATTGTTGGAGAGAATAGCAAACATCCATAATACATTTTTTTTTT420.10561520859003695No Hit
ATAACCGGGCATTTCACACCTCTATTACTTCATTTGAAGTGGTTTTAATATA420.10561520859003695No Hit
GCACTCGGGTTATCCATAGATTTTGAAATATTTCAGTAGTGATGAAGAAAAG410.10310056076646466No Hit
TATCTTGGGGGGCCATCTAGCCCGCCGCTAATCATCCTCTTCCCTTGAATTC410.10310056076646466No Hit
GTTGACGGGAAGGTGCCTGTTGGGTGCAGGGTTCTGTTGGCTCCAGTGATAT410.10310056076646466No Hit
ACAGCAGGGAGAGGGAGGCAGTGCCCAGGCATGTCCCTCCAGGGCCTGGCCT410.10310056076646466No Hit
TCTTTCGGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTT410.10310056076646466No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT410.10310056076646466No Hit
ATAAACGGGACCCATTTGAGTGATATTGCACAACAAACACAGATACCTACAG410.10310056076646466No Hit
AATCAAGGGCAGCTCACTGCAAACTCTGCCTCTGGGGTTCAAGCAATTCTCG410.10310056076646466No Hit
CCAACAGGGAAGATATCAATAGAGAAAAATCAAAGAAATCAACTAGTTCTCT410.10310056076646466No Hit
CTGTGGGGGGAAAAATTAATTAGAGAAATCACTCTTACTGTTGTTAAAGTTA410.10310056076646466No Hit
TCCCTACCTCCCCATCCCCACCTTCTACTCAGAAAACAACCCCTCTCTGTAA410.10310056076646466No Hit
TCTCCAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG410.10310056076646466No Hit
TGTAGTGGGACTGTATATTGTTCAGGGCTATATATCTGTACAAACTTTAAGC410.10310056076646466No Hit
ATCTTGGGGGGAGTCCGAGGTAACAGAACACCTGTCCCCCTAGGCTTTTCCT410.10310056076646466No Hit
AGGCAGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG400.10058591294289235No Hit
AGCATTGGGGGAGCCTGAGTGGCGCCGGGCCCGACGTGGGGCTCCTGGGCCG400.10058591294289235No Hit
GCCTAAGGGCAGTTTTGTCATCCTGGGAGCCTGATATTGGCCGTGATGGAAA400.10058591294289235No Hit
CGCAATGGGACTATGGCACCTATTTGCTGATTTTTGGAGCATTTTTAATTTA400.10058591294289235No Hit
GTATTTGGGACAGGTTAAATATCTTCTTGTGGGGTTAAGGGGTAGAACCTAT400.10058591294289235No Hit
TGCTGCGGGCAGTACTCAGAGGAAAGTTCATAGCATTAAATGCTTACATCAA400.10058591294289235No Hit
TTCTATGGGAATGAAGTAGATGCTGATGGTAATGGCACAATTGACTTCCCTG400.10058591294289235No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)