FastQCFastQC Report
Sat 31 Dec 2022
SRR5849372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40016
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT2750.6872251099560176No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC2670.6672331067572971No Hit
AAGGCTGGGAACCAGACTCAAAAAGAAAGAAAAGAAATCAGAATGTTCAAGA720.1799280287884846No Hit
TTCATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC670.1674330267892843No Hit
AGGCAGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG610.1524390243902439No Hit
ATACTAGGGACAAATCATCATCTTACAAAGGACTAATATCCAGAATCTACAA610.1524390243902439No Hit
AAGCAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA580.14494202319072372No Hit
GTTCGGGAAATACAAGTAATGTCCCTGGGTTAGGTTTCACAAATACATATTT560.1399440223910436No Hit
ACAGCAGGGAGAGGGAGGCAGTGCCCAGGCATGTCCCTCCAGGGCCTGGCCT540.13494602159136346No Hit
ACAAGCGGGTGATTACTAAATCAGAATTCATTTAGCTCACCACATCTCTTGA540.13494602159136346No Hit
GAGTCCGGGCACAGGACTATTCCTAGCCATGCACTACTCACCAGACGCCTCA540.13494602159136346No Hit
TTGCAAGGGATTATTTAATCTTGCAAAAATGGTTCTGTGCACTTGGATGTGA530.1324470211915234No Hit
TGGCACTGGGGGACTCGAATGGAATGGAATGGAATTCACTCGAAAGGAATTG530.1324470211915234No Hit
AACTGGGGGGCGCCCGCTGGCCGGGCTGGCTGCGGCCGCCCTGGGCCGGGCC520.12994802079168333No Hit
GTATTAGGGCGGGGGGCGAGAGCGGCTAGGAGGGCCCGGGCCCGGCGTGGGG520.12994802079168333No Hit
CAGCGAGGGTATGCTTCGTTCAGTCCTTACTGAACATCAGAGACTTCATACT510.12744902039184325No Hit
TAGACAGGGGAGCACTGGAGAGTGTCATGGAGGCCTACGAGCAGGTCCAAAA500.12495001999200321No Hit
TATTCCGGGGGGACATGAACAGTCCTATAGATGAAAAGAGGTGCCTGTCTGA490.12245101959216315No Hit
AGTTTTGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGC490.12245101959216315No Hit
ATCTTGGGGGGAGTCCGAGGTAACAGAACACCTGTCCCCCTAGGCTTTTCCT490.12245101959216315No Hit
TAGTCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC480.11995201919232307No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCCTGTCTCTTATAC480.11995201919232307No Hit
TCTTACGGGACTATTTGAGTGCAGAAGCTCAACCACTTATAAATTTACCAAA470.117453018792483No Hit
GAACAAGGGAGTGCAAAAAGTAATGATAGTGAATGTGATACCATACTTAACT470.117453018792483No Hit
TAAGTGGGGATTCTTAAACTTCAGGAAGTTCTTGCCAATATCAGGAAATATA470.117453018792483No Hit
CTTAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC460.11495401839264295No Hit
CTTGGCGGGAGTGGTAACTCTTTCCAACCGTAGCAGGGTTGTTTTCTGTTAA460.11495401839264295No Hit
CACTGCGGGACTTTTCAAGTGTTTTGATCGTAATCAAGATGTTCATGATAGA460.11495401839264295No Hit
GCATTAGGGAGTTCTGGCTTGTGTAGAGCAGTAAGCAGTGCTTTGTGGGAGG460.11495401839264295No Hit
TTAGTCGGGGAAGGAAATCCCCTGATAATTACTTTGATTCTGAAAATGTTGA460.11495401839264295No Hit
TATGTGGGGAGAAGAATTAAATGAAACATCCATATTTAAATAAGTTGTTGGT460.11495401839264295No Hit
AAGCTCGGGAGCTACATGAGGACTCAGTTGTCTCACTAATATATGTGAAATA450.11245501799280289No Hit
GTCTTTATATTGTTGGAGAGAATAGCAAACATCCATAATACATTTTTTTTTT450.11245501799280289No Hit
GGAAAAGGGGAGAGATGTTTTAAAGGCTTCTGGGTTTGATTTCTTAGTCAGA440.10995601759296282No Hit
TATTCGGGGAGCATACCCTGTAAAAAGTGTGTGGTGGTTGGTAATGGAGGAG440.10995601759296282No Hit
GTAATTGGGGAAAAAACAAATATCCCAAAAAGTCAACCTAAATGTTTTGGAT440.10995601759296282No Hit
ATCACAGGCGCCCTGATCGGCGCACTGCGAGCAGTAGCCCAAACAATCTCAT440.10995601759296282No Hit
GTACCAGGGGACATTAAAACAAACAAATAAGCATCCTTAAGTATCAAATTGA440.10995601759296282No Hit
CCAACAGGGAAGATATCAATAGAGAAAAATCAAAGAAATCAACTAGTTCTCT440.10995601759296282No Hit
GCCGCAGGGAGTCTCATCCATGTTGCCACCAAGAATGACAGAATTTCATTAT440.10995601759296282No Hit
TCAGCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA440.10995601759296282No Hit
TAATCTGGGAACATAGAATCATCATATGATCCAGTGTTCCATACCTAGATAT440.10995601759296282No Hit
CTTATCGGGTATTTAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAAT430.10745701719312274No Hit
TTTAGCAGGGCGATCTAATAGCGGGAATACAACTAAATTTCTGTAATAGTAA430.10745701719312274No Hit
GCTTAAGGGATGGCTTATAAAAAATATGCTACATTAGGAATTCCTTCATGTA430.10745701719312274No Hit
CAAGGGGAGGTAACAAGGAGCAGGGCTAGTGTTCATTCCTTTATGCATTTCT430.10745701719312274No Hit
CGCGATGGGCTCTGTACAGCGCCCCCTTTCTGTTGTGCTATTGGTTGCAGCT430.10745701719312274No Hit
CCATCTGGGATACTTTATAGTTCAAGATTTTCGGTATATAAAATCTGTCCTT430.10745701719312274No Hit
TAGCATGGGGGTTACTTGGTGTATCTTCTGTTAAAGAGGATTGCACGAGTGT430.10745701719312274No Hit
GCATGTGGGGGGACTAGATTCTAAAATTTATTTGGGACCATGGGAATGATAG430.10745701719312274No Hit
GCCCATGGGATTGTCATTCATCTCACTCTGGGCACCTGAGGTCCATAAGCGT420.1049580167932827No Hit
CTTAACGGGAGCTTTGAAACTCCTTAGAGGCTTGTTCAGTGGCCTTTGAGTG420.1049580167932827No Hit
GCATTAGGGAACAAGTTCCAGAGCCACATCATCATCAGGTTTGACCTGAAGA420.1049580167932827No Hit
ACTGGCGGGATAGAATTTTTGTCTAGAATCCCAAACTGGCAACGATAGTCTC420.1049580167932827No Hit
GATAATGGGGGTAAAATAGTTGATGATTCTGTAAGCTTACTAAAACAAATAC420.1049580167932827No Hit
CTGTGGGGGGAAAAATTAATTAGAGAAATCACTCTTACTGTTGTTAAAGTTA420.1049580167932827No Hit
GCTCAAGGGTCTCTCTGAGTCCCGGGCTCGGAGCGCAGGCTCAGCTCCGCGC410.10245901639344263No Hit
GTAATAGGGCTGAGGAAGAGGCCCGTTTGAAGAAGAGTGCAGATACACTTTG410.10245901639344263No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)