FastQCFastQC Report
Sat 31 Dec 2022
SRR5849371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73136
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4740.6481076350907898No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3560.4867643841610151No Hit
ACGCTCGGGATGAGATGTTAGCACTGGGATAGGCTGAGAGAAAGGTACACAG970.13262962152701815No Hit
AAACCAGGGAGTGGAGGCATTGATCGTGAATTTTGAACACAGAAAACCTCAG930.12716035878363596No Hit
ACTTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.12442572741194487No Hit
CAAGCTGGGGAAAGGGACAGTGGAATTTAGCCATACCAAGGACTATACTGGA900.12305841172609933No Hit
TGCTTAGGGATTACAGTTGCCTTTCACATACTCAGAAAGTTAAGTAGAGACA880.12032378035440822No Hit
GGATAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA850.11622183329687158No Hit
GCATTCGGGGAATTCCACAAGAAATTACGATGAGTCCTTCTTCGAGGACCAG850.11622183329687158No Hit
ACACTTGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA830.11348720192518048No Hit
ATCTAGGGGCTTCTTTTATGAAGCTCAGTTTGGCAGAATATAGAATTGTTGA830.11348720192518048No Hit
CTAAATGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG820.11211988623933493No Hit
AACTTTGGGCATCGTTGATAAAAATGCAAAGTCAAATAAAAGGTGTCTTGGT790.10801793918179829No Hit
AGCCTGGGGAGGACAGGAAAAAGAGAAATCTCGACATGGAAAAACCCTACAA790.10801793918179829No Hit
TAATTAGGGGATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGA790.10801793918179829No Hit
TCCTCAGGGAGGAGCTTTCCAATACTCATTTAATTAAGATTTAATCATTTGC790.10801793918179829No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCCTGTCTCTTATAC780.10665062349595274No Hit
TATTGGAGGGAGCGGGAGAGAGAGAGGGAACGTGAACGGGATCGGGAAAGAG770.1052833078101072No Hit
TCTCAGGGATGCAAAAATATGTGTTAGGTCAGTGAAGTTAGAAAAACCTTTC760.10391599212426164No Hit
ACATTTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC760.10391599212426164No Hit
CAATAGGGGAGAACCACTTTCATTACCTTCTAGCAGTATAGAGTTTGGGCAA750.10254867643841609No Hit
TTAGTGGGGAAGACAGTACAAAAACACTGGCACTTGAATGTTGAATGTCACC750.10254867643841609No Hit
AAAGTTGGGAGACATGCAAATGGCCAACAGATATGTGAAAAACAAATGCTCA740.10118136075257056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)