FastQCFastQC Report
Sat 31 Dec 2022
SRR5849370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73249
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGCCGTCT4500.614342857922975No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCGGAGTTAATCTCGTATGC3610.49283949268932004No Hit
ACGCTCGGGATGAGATGTTAGCACTGGGATAGGCTGAGAGAAAGGTACACAG1090.14880749225245396No Hit
AAGCAAGGGGGGCGGAAGAGGTGGCCCCGGATGCTGCGGCGCCCGCTGGCCG980.13379022239211458No Hit
TGCTTAGGGATTACAGTTGCCTTTCACATACTCAGAAAGTTAAGTAGAGACA930.12696419063741485No Hit
GCATAGGGAGGAAAAACTCAATAAAACCAAGAGTTAGTTCTTCGAAAAAATA920.1255989842864749No Hit
TAGCTGTGGGTGGCTCGGGGGTCACCATTATCAATGCTACTGAGGAGAAAAG910.12423377793553496No Hit
AGAAAGGACTACAGAGCCCCGAATTAATACCAATAGAAGGGCAATGCTTTTA910.12423377793553496No Hit
ATCTAGGGGCTTCTTTTATGAAGCTCAGTTTGGCAGAATATAGAATTGTTGA880.12013815888271512No Hit
GGATAAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA870.11877295253177518No Hit
TAATTAGGGGATTCTAGCCACCTCTAGCCTAGCCGTTTACTCAATCCTCTGA850.11604253982989529No Hit
ATCCTGGGCAAGTGCTATGTAAATGCTAATTGTATCAATTAATATTACTGCC840.11467733347895535No Hit
ACTTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.11058171442613551No Hit
AAAGTTGGGAGACATGCAAATGGCCAACAGATATGTGAAAAACAAATGCTCA770.10512088902237572No Hit
GCACAGGGGAATTCTTCACAACTAAATATATCTAGTTAATTTTAACTTTAGG770.10512088902237572No Hit
CATGCTGGGCTGTCATCTGGAAGCTTTACACTATTTCCTAAGGCCATGATTC770.10512088902237572No Hit
TGACCAGGGAGGCAGGAAGCCATTTGATTTCTATTATTTTCCTGACTTTGAA770.10512088902237572No Hit
TGCTGGGGGACACCAGAGGTGGCCGAATCATCTCCACTTCTTCGCGTTCGGT760.10375568267143578No Hit
TCTGTTGGGAAGGAAAGTTCCCAAGACCTGGGAAAGAATAAAGCTGAAATGA750.10239047632049583No Hit
CTTTGAGGGACAGAAATGTTTGTAGCAGCTTTATTCATGATTGCAAGAACTA750.10239047632049583No Hit
TCTAAAGGGAGGTGCAGCTTTTCCATATCAGCTCAACCATGCCGCCAGTCCA740.1010252699695559No Hit
CCGCCCGGGGGCCCACGTGGCCTCCGCGGGCCCCGCCAGAGCCTGCGCCCGG740.1010252699695559No Hit
GCATTCGGGGAATTCCACAAGAAATTACGATGAGTCCTTCTTCGAGGACCAG740.1010252699695559No Hit
TATTGGAGGGAGCGGGAGAGAGAGAGGGAACGTGAACGGGATCGGGAAAGAG740.1010252699695559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)